不同投喂方式对中国明对虾幼体生长发育及抗病力的研究

研究β-1,3/1,6-D-glucan及不同投喂方式对中国明对虾(Fenneropenaeus chinensis)幼体的存活、生长、发育、非特异性免疫及抗副溶血弧菌能力的影响.将β-glucan以0.25%的剂量添加到微黏合饲料中,以不同的投喂方式投喂对虾幼体,即D0(一直投喂基础饲料);D1(一直投喂含0.25%β-葡聚糖饲料);3D1-3D0(3 d投喂含0.25%β-葡聚糖的饲料-3 d投喂基础饲料);1D1-3D0(1 d投喂含0.25%β-葡聚糖的饲料-3 d投喂基础饲料),共4个处理,每个处理随机设置3个重复,对虾幼体养殖于可控温的半开放系统中,每个养殖桶中盛有50 L盐度为30～32消毒海水,养殖密度为100尾/L.实验结果表明,在孵化后14 d,投喂D1饲料组在总存活率,变态指数,体长,特定生长率方面均高于其它各组,虽然有时差异不显著.在免疫指标方面,在9和14 dph分别进行了酚氧化酶(PO)、碱性磷酸酶(ALP)、超氧化物歧化酶(SOD)、酸性磷酸酶(ACP)活力的测定,结果表明,投喂D1饲料组的4个免疫指标均高于其它3组,虽然有时差异不显著.在14 dph的攻毒实验结果表明,对照组的累积死亡率显著高于D1组(P<0.05),而1D1-3D0和3D1-3D0的累积死亡率则与其它各组均不显著.实验结果表明,间隔投喂方式并不适合于对虾幼体,在中国明对虾幼体饲料中使用β-葡聚糖时,建议添加量为0.25%,并采用一直投喂的方式.     

西北太平洋上层热含量的时空变化

基于全球月平均海温资料、137°E断面海温观测资料、同化水位资料和太平洋850 hPa纬向风资料,利用EOF、功率谱和最大熵谱等分析方法,分析了西北太平洋上层热含量的时空变化,并讨论了热含量变化与水位和赤道太平洋纬向风异常的关系.结果表明,西北太平洋上层热含量具有明显的年际和年代际变化;热含量的年际变化与热带太平洋大尺度海气系统异常相联系,即在El Ni(n)o期间,热含量减少,而在La Ni(n)a期间热含量增多;热含量在20世纪70年代末发生了一次气候跃变,在跃变前热含量偏多,而在跃变后则偏少;热含量与水位间存在着非常一致的同位相年际变化,而这种变化与赤道西、中太平洋的纬向风异常有关.     

三维旋转扫描测量平台设计与实现

本设计主要用于360°旋转的三维扫描,实现角度数据的实时获取。设计中采用单片机控制交流伺服电机进行自定的角度、速度与方向的旋转并实时上传时间、角度数据至上位机进行处理,实现了对平台旋转精度的控制与检测,着重研究了该传感器误差源的产生,对实际应用中的误差进行了定量分析,利用偏心改正、4分频等方法减少了误差,提高了参数精度。角度范围为360°,角度分辨率为0.01°。     

Variations in the concentration of mesospheric ozone during the total solar eclipse of March 29, 2006, from microwave radiometric data

We present the results of microwave observations of the ozone content variability in the upper stratosphere and lower mesosphere during a total solar eclipse of March 29, 2006 at the Kislovodsk high-altitude scientific station. An increase in the concentration of mesospheric ozone was recorded during the eclipse. At a height of 60 km, the ozone concentration increased by 40%, which is close to the value of diurnal ozone variations.     

Influence of the sea surface temperature conditions in the Pacific subarctic frontal zone on the pink salmon’s winter survival ability

Similar to other high-latitude ocean basins, the subarctic Pacific exhibits strong seasonal and inter-annual variations in the abiotic factors of the environment, which, in turn, strongly influence biological objects. One of the principal factors of this kind is the temperature regime. For our research, we chose pink salmon because more than 90% of its natural mortality occurs precisely during the wintering period. The lifetime of pink salmon is only one year, and the conditions of their populations reflect the thermal regime of the given year. The main wintering area of Asian pink salmon is the part of the subarctic frontal zone located south of the Aleutian Islands (43°–46°N). This region features sufficiently high wintertime concentrations of chlorophyll a and temperature conditions favorable for pink salmon wintering. The interannual temperature variability in the frontal zone is close to zero, and the width of the frontal zone may significantly change depending on the winter severity. In “milder” winters, the area of wintering extends, while, in “severe” winters, it is rather narrow, the forage base for fish decreases, they become more accessible for predators, and their survival rates sharply drop.     

The study on a new technology of waterway information system

It is a lot limitations to survey and design waterway by the CAD technique,due to timely variation of hydrology and ecology in the complicated estuary area,such as Changjing River estuary.In this paper,the advantages of GIS technique is applied to manage and process the multi kind,multi scale and multi time hydrographic parameters,including water depth,current velocity,tidal,waves,wind speed,temperature and salinity etc.,also to deal with different projection,different scale charts.Meanwhile,in the paper,combination techniques of GIS and CAD has been developed for unification of the charts for efficient management,two-way automatic carts conversion between GIS and CAD,automatic cutting and assemble of a number of Charts,flight path of the projection of spatial data and coordinate transformation.This new technique has been applied to survey and design waterway of Changing River estuary.The result shows that the combination technology of GIS and CAD has application potentiality in the waterway survey and design.     

海洋毒素大田软海绵酸完全抗原的制备与分析

为了建立大田软海绵酸(Okadaic acid,0A)的免疫学检测方法,利用活泼酯法将OA分子中的羧基与栽体蛋白上的氨基偶联,人工制备OA的免疫抗原(OA-IgG)和检测抗原(OA-BSA),经电泳、动物实验及ELISA法进行了鉴定,完全抗原的偶联成功,为利用杂交瘤技术制备OA的单克隆抗体和海产品OA免疫学检测方法奠定了良好的基础.     

Elastic-plastic analysis for pile-anchor supporting system of deep foundation pit

By using numerical analysis methods to simulate the deep excavation, a lot of analyses are established on the basis of two-dimensional plane strain, ignoring the fact that foundation pit possesses three dimensions. For soil constitutive relation, people always take linear and nonlinear model, without considering the plastic behavior of soil. Using plastic-elastic hardening model to simulate constitutive relation of soil characteristics, the authors carried out mechanical analysis for pit excavation and support. The results show that the analysis for the stress state of pile anchor system is an effective way which provides theoretical basis for calculation of soil displacement.