Effects of the inhibitor ellipticine on cytochrome P450-reductase and cytochrome P450 (1A) function in hepatic microsomes of flounder (Platichthys flesus)

The effects of the mammalian inhibitor ellipticine (5,11-dimethyl-[6H]-pyrido[4,3b] carbazole) were examined in a mechanistic study of the cytochrome P450 monooxygenase system of control and β-naphthoflavone (βNF)-induced hepatic microsomes of Platichthys flesus. Ellipticine was indicated to bind to the haem moiety of cytochrome P450s (gave type II binding spectra) and to inhibit the transfer of electrons from both the hydrophobic binding site of cytochrome P450 reductase (P450R) to P450 (inhibited P450R reductase activity) and the hydrophilic binding site of P450R to soluble electron acceptors (inhibited NAD(P)H-cytochrome c reductase activity). No effect was seen on cytochrome b₅ reductase activity. Ellipticine inhibition indicated the involvement of (i) P450R (possibly also P450s) in NADPH- but not NADH- dependent hydroxyl radical production, and (ii) electron transfer and P450/P450R interaction in NADPH-dependent cytochrome P450 1A-catalysed monooxygenation (7-ethoxyresorufin O-deethylase activity and benzo(a)pyrene (BaP) metabolism). Differential effects of ellipticine on cumene hydroperoxide (CHP)-dependent BaP metabolism (P450 peroxidase activity) with CHP concentration indicated the existence of at least two forms of P450 with different substrate affinities for CHP, and different mechanisms of formation for protein adducts and free metabolites. Overall, the studies indicate the primary site of action of ellipticine in P. flesus is binding between Fe³⁺-P450 and P450R.     

Accretion,structure and hydrology of intermediate spreading-rate oceanic crust from drillhole experiments and seafloor observations

Downhole measurements recorded in the context of the Ocean Drilling Program in Hole 504B, the deepest hole drilled yet into the oceanic crust, are analyzed in terms of accretion processes of the upper oceanic crust at intermediate spreading-rate. The upper part of the crust is found to support the non steady-state models of crustal accretion developed from seafloor observations (Kappel and Ryan, 1986; Gente, 1987). The continuous and vertical nature of borehole measurements provides stratigraphic and structural data that cannot be obtained solely from seafloor studies and, in turn, these models define a framework to analyze the structural, hydrological, and mineralogical observations made in the hole over the past decade.Due to the observed zonation with depth of alteration processes, and its relation to lava morphologies, the 650-m-thick effusive section penetrated in Hole 504B is postulated to be emplaced as the result of two main volcanic sequences. Massive lava flows are interpreted as corresponding to the onset of these sequences emplaced on the floor of the axial graben. The underlying lava made of structures with large porosity values and numerous cm-scale fractures is thus necessarily accreted at the end of the previous volcanic episode. On top of such high heterogeneous and porous intervals, the thick lava flows constitute crustal permeability barriers, thereby constraining the circulation of hydrothermal fluids.Accreted in the near vicinity of the magma chamber, the lower section is that exposed to the most intense hydrothermal circulation (such as black smokers activity). Once capped by a massive flow at the onset of the second volcanic phase, the lower interval is hydrologically separated from ocean-waters. A reducing environment develops then below it resulting, for example, in the precipitation of sulfides. Today, whereas the interval corresponding to the first volcanic episode is sealed by alteration minerals, the second-one is still open to fluid circulation in its upper section. Thus, upper part of the volcanic edifice is potentially never exposed to fluids reaching deep into the crust, while the lower one is near the ridge axis.Considering that most of the extrusives are emplaced within a narrow volcanic zone, the first unit extruded for a given vertical cross-section is necessarily emplaced at the ridge-axis. In Hole 504B, the 250-m-thickTransition Zone from dikes to extrusives is interpreted as the relict massive unit flooding the axial graben at the onset of the first volcanic sequence, and later ruptured by numerous dikes. Further from the axis, the same massive unit constitutes a potential permeability cap for vertical crustal sections accreted earlier. Also, the upper 50 meters of the basement might be considered as the far-end expression of massive outpours extruded near the ridge-axis.     

The Role of Laser Ranging for Calibrating Jason-1: The Corsica Tracking Campaign

The French Transportable Laser Ranging System (FTLRS), a highly transportable Satellite Laser Ranging (SLR) instrument, was set up in Corsica (from January to September 2002) for participating to the JASON-1 altimeter verification phase. In addition to the tracking of oceanographic satellite missions and in order to perform an accurate positioning, the FTLRS also acquired laser ranging data on geodetic satellites, STARLETTE and STELLA essentially.

The paper describes the analysis strategy mainly based on the use of a short-arc orbit technique to compute accurate 1 cm local orbits, and then the geocentric positioning (2-3 mm relative to GPS). Finally, we established the JASON-1 absolute calibration value, based on 9 SLR short-arcs (between cycles 1 and 26), at 108.2 ± 8.7 mm; the 10-day repeatability is of 26.1 mm showing that a great accuracy has been reached.     

Lower cretaceous basalt and sediments from the Kerguelen Plateau

Geological samples from the southern Kerguelen Plateau include Lower Cretaceous basalt and lava breccia, probable Lower Cretaceous conglomerate and shelf limestone, Upper Cretaceous chert with dolomite, Upper Cretaceous-Eocene ooze, and Tertiary conglomerate. Neogene sediments are only a few hundred m thick, and include foraminiferal and diatomaceous ooze, and ice-rafted debris. In conjunction with seismic reflection profiles, the samples indicate Early Cretaceous near-shore volcanism, followed by erosion, sedimentation, and subsidence through Cretaceous; arching of the plateau at the end of Cretaceous; subsidence through Paleogene; widespread emergence in mid-Tertiary; and slow subsidence through Neogene.     

Mantle Bouguer Anomaly Along an Ultra Slow-Spreading Ridge: Implications for Accretionary Processes and Comparison with Results from Central Mid-Atlantic Ridge

A three-dimensional analysis of gravity andbathymetry data has been achieved along the Southwest Indian Ridge (SWIR)between the Rodriguez Triple Junction (RTJ) and the Atlantis II transform,in order to define the morphological and geophysical expression ofsecond-order segmentation along an ultra slow-spreading ridge(spreading rate of 8 mm/yr), and to compare it with awell-studied section along a slow-spreading ridge (spreadingrate of 12.5 mm/yr): the Mid-Atlantic Ridge (MAR) between 28°and 31°30 N.Between the Atlantis II transform and theRTJ, the SWIR axis exhibits a deep axial valley with an 30°oblique trend relative to the north–south spreading direction. Onlythree transform faults offset the axis, so the obliquity has to beaccommodated by the second-order segmentation. Alongslow-spreading ridges such as the MAR, second-order segmentshave been defined as linear features perpendicular to the spreadingdirection, with a shallow axial valley floor at the segment midpoint,deepening to the segment ends, and are associated with Mantle BouguerAnomaly (MBA) lows. Along the SWIR, our gravity study reveals the presenceof circular MBA lows, but they are spaced further apart than expected. Thesegravity lows are systematically centred over narrow bathymetric highs, andinterpreted as the centres of spreading cells. However, along some obliquesections of the axis, the valley floor displays small topographicundulations, which can be interpreted as small accretionary segments frommorphological analysis, but as large discontinuity domains from thegeophysical data. Therefore, both bathymetry and MBA variations have to beused to define the second-order segmentation of an ultraslow-spreading ridge. This segmentation appears to be characterisedby short segments and large oblique discontinuity domains. Analysis of alongaxis bathymetric and gravimetric profiles exhibits three different sectionsthat can be related to the thermal structure of the lithosphere beneath theSWIR axis.The comparison between characteristics of segmentationalong the SWIR and the MAR reveals two major differences: first, the poorcorrelation between MBA and bathymetry variations and second, the largerspacing and amplitude of MBA lows along the SWIR compared to the MAR. Theseobservations seem to be correlated with the spreading rate and the thermalstructure of the ridge. Therefore, the gravity signature of the segmentationand thus the accretionary processes appear to be very different: there areno distinct MBA lows on fast-spreading ridges, adjacent ones on slowspreading ridges and finally separate ones on ultra slow-spreadingridges. The main result of this study is to point out that 2nd ordersegmentation of an ultra slow-spreading ridge is characterised bywide discontinuity domains with very short accretionary segments, suggestingvery focused mantle upwelling, with a limited magma supply through a cold,thick lithosphere. We also emphasise the stronger influence of themechanical lithosphere on accretionary processes along an ultra slow-spreading ridge.     

Predicting the impact of land use on the major element and nutrient fluxes in coastal Mediterranean rivers: The case of the Têt River (Southern France)

This study presents a detailed discrimination between the natural and anthropogenic sources of dissolved major elements in the Têt River, a typical small coastal river in the south of France. The main objectives were to quantify the materials that were released by human activities in the basin, and to determine the specific element inputs for the major land use forms. The dissolved material fluxes were estimated by weekly monitoring over a hydrological year (2000–2001) along the major water gauging stations, and the flux relationships were examined in the context of anthropogenic and natural basin characteristics as determined by a Geographical Information System (GIS). Intensive agricultural land use in the form of fruit tree cultures and vineyards has a strong control on the dissolved element fluxes in the river. Area specific element releases for these cultures are greatest for SO₄, with an estimated average of about 430 ± 18 keq km⁻² a⁻¹. This is ?11 times the natural SO₄ release by rock weathering. Also for K, NO₃, PO₄ and Mg, the specific releases were ?6 times the natural weathering rates (respectively about 44, 60, 4 and 265 keq km⁻² a⁻¹). Waste-waters are the other major source of anthropogenic elements in the river. They have an important role for the fluxes of inorganic P and N, but they are also a considerable source of Cl and Na to the river. For example, the average annual release of Cl is around 150 moles/inhabitant in the rural basin parts. Further downstream, however, where population density strongly increases, industrial effluents can enhance this value (>300 moles/inhabitant). The waste-waters contribute more than 70% of the dissolved inorganic N export to the sea, although their contribution to the average DOC export is almost negligible (3%).     

Palaeozoic oolitic ironstone of the French Armorican Massif: a chemical and structural trap for orogenic base metal–As–Sb–Au mineralisation during Hercynian strike-slip deformation

In the Saint-Aubin-des-Chateaux quarry (Armorican Hercynian belt, western France), an epigenetic hydrothermal alteration affects an oolitic ironstone layer intercalated within the Lower Ordovician Grès armoricain Formation. The hydrothermal overprint produced pervasive and massive sulphidation with stratoid pyritised lenticular bodies within the oolitic ironstone layer. These sulphide lenses are spatially associated with strike-slip faults and extend laterally from them. After the massive sulphidation stage (Fe–As, stage 1), subsequent fracturing allowed the deposition of base metals (stage 2) and Pb–Sb–Au (stage 3) parageneses in veins. The dominant brittle structures are vertical extension veins, conjugate shear veins and strike-slip faults of various orders. All these structures are filled with the same paragenetic sequence. Deformation analysis allows the identification of structures that developed incrementally via right-lateral simple shear compatible with bulk strain affecting the Central Armorican Domain. Each increment corresponds to a fracture set filled with specific parageneses. Successive hydrothermal pulses reflect clockwise rotation of the horizontal shortening direction. Geothermometry on chlorite and arsenopyrite shows an input of hot hydrothermal fluids (maximum of 390–350°C) during the main sulphide stage 1. The subsequent stages present a marked temperature drop (300–275°C). Lead isotopes suggest that the lead source is similar for all hydrothermal stages and corresponds to the underlying Neo-Proterozoic basement. Lead isotope data, relative ages of deformation and comparison with neighbouring deposits suggest that large-scale fluid pulses occurred during the whole Hercynian orogeny rather than pulses restricted to the late Hercynian period. The vicinity of the Hercynian internal domain appears as a key control for deformation and fluid flow in the oolitic ironstones, which acted as a chemical and structural trap for the hydrothermal fluids. The epigenetic mineralisation of Saint-Aubin-des-Chateaux appears to be very similar to epigenetic sulphidation described in banded iron formation-hosted gold deposits.