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长牡蛎碱性磷酸酶的分离纯化及部分性质研究 总被引:5,自引:1,他引:5
本文以长牡蛎为酶源提取材料,用正丁醇抽提,硫酸铵分级分离,DEAE-52和Sephadex G-100柱层析,得到一定纯度的碱性磷酸酶,提纯倍数为61.96,比活力达1.326U/mg.该酶的最适pH值9.6,最适温度38℃,初速度6.8μmol/(dm~3·min),米氏常数Km值为1.30mmol/dm~3.实验结果表明,Mg~(2+)对该酶有明显的激活作用;Zn~(2+)、Cu~(2+)、Hg~(2+)对该酶有明显的抑制作用. 相似文献
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The present paper deals with the ultrastructural changes of oocyte in the process of maturing in pen shell Atrinapectinata Linne. When it develops from the antecedent growth period to the accelerating growth period the annulate lamellae emerge, the RER better develop, the yolk granules with single and double layer membrane and are visible. In the well-developed mitochondria there is yolk stored. The Golgi vacuoles are brewing yolk granules. At the mature period the annulate lamellae and the Golgi bodies disappear, and there are only a few RER and mitochondria with poor cristae in the cytoplasm. All these might be well used for judging the maturity of the ootids in the process of the artificial propagation. 相似文献
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M. -S. Jiang F. Chai R. C. Dugdale F. P. Wilkerson T. -H. Peng R. T. Barber 《Deep Sea Research Part II: Topical Studies in Oceanography》2003,50(22-26):2971
A coupled physical–biological model was developed to simulate the low-silicate, high-nitrate, and low-chlorophyll (LSHNLC) conditions in the equatorial Pacific Ocean and used to compute a detailed budget in the Wyrtki box (5°N–5°S, 180–90°W) for the major sources and cycling of nitrogen and silicon in the equatorial Pacific. With the incorporation of biogenic silicon dissolution, NH4 regeneration from organic nitrogen and nitrification of ammonia in the model, we show that silicon recycling in the upper ocean is less efficient than nitrogen. As the major source of nutrients to the equatorial Pacific, the Equatorial Undercurrent provides slightly less Si(OH)4 than NO3 to the upwelling zone, which is defined as 2.5°N–2.5°S. As a result, the equatorial upwelling supplies less Si(OH)4 than NO3 into the euphotic zone in the Wyrtki box, having a Si/N supply ratio of about 0.85 (2.5 vs. 2.96 mmolm−2 day−1). More Si(OH)4 than NO3 is taken up with a Si/N ratio of 1.17 (2.72 vs. 2.33 mmolm−2 day−1) within the euphotic zone. The difference between upwelling supply and biological uptake is balanced by nutrient regeneration and horizontal advection. Excluding regeneration, the net silicate and nitrate uptakes are nearly equal (1.76 vs. 1.84 mmolm−2 day−1). However, biogenic silica export production is slightly higher than organic nitrogen (1.74 vs. 1.59 mmolm−2 day−1) following a 1.1 Si/N ratio. In the central equatorial Pacific, low silicate concentrations limit diatom growth; therefore non-diatom new production accounts for most of the new production. Higher silicate supply in the east maintains elevated diatom growth rates and new production associated with diatoms dominate upwelling zone. In contrast, the new production associated with small phytoplankton is nearly constant or decreases eastward along the equator. The total new production has a higher rate in the east than in the west, following the pattern of surface silicate. This suggests that silicate regulates the diatom production, total new production, and thereby carbon cycle in this area. The modeled mean primary production is 48.4 mmolCm−2 day−1, representing the lower end of direct field measurements, while new production is 15.0 mmolCm−2 day−1, which compares well with previous estimates. 相似文献
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通过分析1980年以后的海冰资料和卫星云图照片,对黄、渤海沿岸11个海冰测点的历史变迁进行了评述,浅析了测点的优劣及使用资料时应注意的事项。 相似文献
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根据1992年12月(冬季)和1993年7月(夏季)对大鹏澳海域24h定点连续观测和夏季大面调查,统计了该海域各种形态磷的变化范围和平均含量;计算了各种形态磷分别占总磷(TP)和总溶解磷(TDP)的百分比;讨论了各种形态磷之间的相互关系;分析了影响TP和TDP分布的环境因素。 相似文献
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The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy. 相似文献