外源CaCl2调控浒苔(Ulva prolifera)高温逆境的比较转录组研究

钙(Ca~(2+))作为一种必需的信号分子,在植物的生长发育和非生物胁迫调节中起着至关重要的作用。本实验采用BGISEQ平台进行高通量测序,获得浒苔外源CaCl_2添加(UpCa)和高温对照(UpHT)处理下相关的转录组数据,分析了在高温(35℃)下外源CaCl_2的添加转录组的变化。结果显示,根据UpHT和UpCa处理分析共获得36625条基因;与UpHT相比,在UpCa下鉴定出7513个差异表达的基因,包括6002个上调基因, 1151个下调基因,对差异基因进行了GO富集和KEGG富集分析。对膜转运、植物信号转导和环境适应性相关的差异基因进行KEGG富集分析,主要富集在内吞(Endocytosis)、植物病原菌互作(Plant-pathogeninteraction)、丝裂原激活的蛋白激酶信号通路(MAPK signaling pathway-plant)、植物激素的信号传递(Plant hormone signal transduction)、ABC转运(ABCtransporters)和磷脂酰肌醇信号系统(Phosphatidylinositolsignalingsystem)等代谢通路上。植物激素信号转导途径中,细胞分裂素、脱落酸、油菜素内酯和乙烯信号转导途径增强。抗氧化酶中FeSOD、谷胱甘肽过氧化物酶、谷胱甘肽S转移酶和抗坏血酸过氧化物酶等基因表达上调,下调的基因主要是热激蛋白。Ca~(2+)信号组分基因钙结合蛋白、钙调素、钙/钙调素依赖的蛋白激酶以及钙/钙调蛋白依赖性3′,5′环核苷酸磷酸二酯酶的基因表达上调,丝裂原活化蛋白激酶激酶激酶和磷脂酰肌醇信号相关的基因也差异性上调。本研究系统阐述了植物信号转导、抗氧化酶、MAPK信号系统以及Ca~(2+)信号组分基因在外源CaCl_2对浒苔高温压力的调节中的重要作用,可为进一步阐明Ca~(2+)信号对高温的调节适应机制提供理论基础。     

黄河三角洲潮间带大型底栖无脊椎动物群落结构与分布特征

为了解黄河三角洲潮间带大型底栖无脊椎动物现状,于2019年5月分别对建国后黄河入海口4个流路区域20个潮间带站位进行了采样调查.调查共采集到大型底栖无脊椎动物5纲16目37科60种,主要优势种有托氏（虫昌）螺(Umbonium costatum)、泥螺(Bullacta exarata)、光滑河篮蛤(Potamocor...     

A New Retracking Technique for Brown Peaky Altimetric Waveforms

A new Brown-Peaky (BP) retracker has been developed for peaky waveforms that usually appear within ～10 km to the coastline. The main feature of the BP is that it fits peaky waveforms using the Brown model without introducing a peak function. The retracking strategy first detects the peak location and width of a waveform using an adaptive peak detection method, and then estimates retracking parameters using a weighted least squares (WLS) estimator. The WLS assigns a downsized weight to corrupted waveform gates, but an equal weight to other normal waveform gates. The BP retracker has been applied to 4-year Jason-1 waveform (2002–2006) in two Australian coastal zones. The results retracked by BP, MLE4 and ALES retrackers have been validated against tide-gauge observations located at Burnie, Lorne and Broome. The comparison results show that three retrackers have similar performance over open oceans with the correlation coefficient (～0.7) and RMSE (～13 cm) between altimetric and tide-gauge sea levels for distance >7 km offshore. The main improvement of BP retracker occurs for distance ≤7 km to the coastline, where validation results indicate that data retracked by BP are more accurate (15–21 cm) than those by ALES (16–24 cm) and MLE4 (19–37 cm).     

南海西沙永乐龙洞沉积物组成、来源及其沉积作用

南海永乐龙洞发育于永乐珊瑚礁台地,龙洞深度达300m,为世界之最。沉积物堆积在龙洞的洞壁斜坡、龙洞中部的转折平台以及洞底等部位。使用激光粒度仪、X射线粉晶衍射仪、X射线荧光光谱仪等对采自不同深度的沉积物进行了粒级、矿物物相、元素含量的研究。研究结果表明:龙洞沉积物绝大部分为钙质生物碎屑,以砂粒级碎屑为主,含砾石碎屑、粉砂碎屑,分选和磨圆差;沉积物矿物组成以文石、高镁方解石为主,含少量低镁方解石,其平均含量分别为69%、28%、3%;化学组成以Ca、Mg、Sr为主,平均含量分别为35.5%、0.9%、0.5%,含少量Si、Al、Ti、P、S等元素。该区沉积物来源包括礁坪生物碎屑和东亚季风风尘陆源物质两个方面,以礁坪来源的生物碎屑为主;龙洞沉积作用包括机械捕获作用和垂直沉降作用两种方式,而以机械捕获作用为主。     

面向海上目标搜索任务的多无人机协同航路优化

提出了一种面向多无人机(unmanned aerial vehicle,UAV)协同搜索海上目标任务的航路优化方法。首先,分析多无人机协同航路优化问题的基本要素模型。然后,在对各UAV独立维护的目标概率图信息进行探测更新的基础上,采用状态预测一致性算法实现目标概率图信息的快速融合。最后,同时考虑局部搜索收益与未来搜索收益,采用分布式模型预测控制(distributed model predictive control,DMPC)方法优化各UAV的搜索航路。仿真结果表明,本研究提出的方法具有较高的搜索效率,可有效应用于海上目标的快速搜索任务,具有重要的应用价值。     

印度尼西亚北苏拉威西蓝碧海峡浮游动物种类组成及分布

Based on oceanographic survey data in June 2012 in the Lembeh Strait, the zooplankton ecological characteristics such as species composition, individual abundance, dominant species and distribution were analyzed. The results showed that 183 species(including 4 sp.) had been recognized, most of them belonged to copepoda.Cnidaria followed with 43 species(including 1 sp.) were identified. The average abundance of zooplankton was(150.47±58.91) ind./m~3. As to the horizontal distribution, the abundance of the zooplankton was higher in the southern waters than in the northern waters. The dominant species in the study area were Lensia subtiloides,Sagitta enflata, Lucifer intermedius, Oikopleura rufescens, Diphyes chamissoni, Creseis acicula, Subeucalanus subcrassus, Temora discaudata, Aglaura hemistoma, Doliolum denticulatum, Canthocalanus pauper, Oikopleura longicauda and Nanomia bijuga. Zooplankton biodiversity indexes were higher in study area than previous study in the other regions. The findings from this study provide important baseline information for future research and monitoring programs.     

鲫鱼(Carassius auratus)半胱亚磺酸脱酸酶CSAD基因克隆及其表达研究

本研究以鲫鱼(Carassius auratus)为研究对象,采用巢式PCR和RACE技术克隆鲫鱼半胱亚磺酸脱酸酶CSAD基因c DNA序列,并且利用实时荧光定量PCR检测了CSAD m RNA在鲫鱼不同组织和昼夜节律中的相对表达水平,同时还研究了牛磺酸对鲫鱼肠道CSAD m RNA表达的影响。结果表明:(1)鲫鱼CSAD基因c DNA序列包含186bp的5?UTR序列,675bp的3?UTR序列,1503bp开放阅读框,编码500个氨基酸。同源性分析表明,鲫鱼和鲤鱼的同源性为97.2%。系统发育分析表明,鲫鱼与鲤鱼之间的亲缘关系最接近,置信度为100。经预测,其编码的蛋白质的分子量和等电点分别为56.82k Da和5.77;(2)Ca CSAD m RNA在肌肉、心脏、肠道及肝脏中的表达水平较高,在脑和鳃组织中的相对表达量较低;(3)鲫鱼肠道CSAD m RNA的相对表达量在6:00am点时最高,9:00pm点时相对表达量最低;(4)鲫鱼CSAD的相对表达丰度随着牛磺酸添加量的增加而逐渐下降。本研究结果不仅有助于理解鲫鱼CSAD基因的分子特征,同时将为进一步研究鱼类CSAD营养调控功能提供理论依据。     

海洋文化产业与旅游产业融合探析

文章分析海洋文化产业与旅游产业的融合过程,主要包括融合动因、融合产业、融合障碍和融合对策。以舟山市为例,对海洋文化产业与旅游产业融合的意义、适宜度和障碍进行问卷调查,结果表明:受众认为海洋文化产业与旅游产业融合的意义主要是带动海洋经济增长、增加就业机会和促进沿海地区发展;海洋农业文化业、海洋公共文艺业、海洋商业演艺业和海洋传媒业与旅游产业的融合适宜度较高;市场是产业融合的主要障碍,保护和发展的矛盾也是重要障碍。基于调查和研究结论,提出促进海洋文化产业与旅游产业融合的建议,即发展海洋文化旅游产业链、政府和市场联动以及加强海洋生态文明建设。     

Hydrological Processes in the Huaihe River Basin,China: Seasonal Variations,Causes and Implications

Understanding streamflow changes in terms of trends and periodicities and relevant causes is the first step into scientific management of water resources in a changing environment. In this study, monthly streamflow variations were analyzed using Modified Mann-Kendall(MM-K) trend test and Continuous Wavelet Transform(CWT) methods at 9 hydrological stations in the Huaihe River Basin. It was found that: 1) streamflow mainly occurs during May to September, accounting for 70.4% of the annual total streamflowamount with Cv values between 0.16–0.85 and extremum ratio values between 1.70–23.90; 2) decreased streamflow can be observed in the Huaihe River Basin and significant decreased streamflow can be detected during April and May, which should be the results of precipitation change and increased irrigation demand; 3) significant periods of 2–4 yr were detected during the 1960 s, the 1980 s and the 2000 s. Different periods were found at stations concentrated within certain regions implying periods of streamflow were caused by different influencing factors for specific regions; 4) Pacific Decadal Oscillation(PDO) has the most significant impacts on monthly streamflow mainly during June. Besides, Southern Oscillation Index(SOI), North Atlantic Oscillation(NAO) and the Ni?o3.4 Sea Surface Temperature(Ni?o3.4) have impacts on monthly streamflow with three months lags, and was less significant in time lag of six months. Identification of critical climatic factors having impacts on streamflow changes can help to predict monthly streamflow changes using climatic factors as explanatory variables. These findings were well corroborated by results concerning impacts of El Nino-Southern Oscillation(ENSO) regimes on precipitation events across the Huaihe River Basin. The results of this study can provide theoretical background for basin-scale management of water resources and agricultural irrigation.     

顾及字符特征的中文地名词典查询方法

地名词典查询是地名校正、地名匹配等地名服务应用的重要基础,但是地名数量的快速增长使得词典查询性能面临严峻挑战。针对大规模数据环境中传统词典查询方法准确率不高且效率较低等问题,提出了一种顾及字符特征的中文地名词典查询方法（CGQM）。首先,查询具有相同字符特征的地名形成候选地名集合,同时构建单字索引提升查询效率;其次,依据字符数量特征比较查询地名与候选地名的差异,进一步过滤候选地名集合;最后,基于字符位置特征优化查询结果排序策略,使得结果排序更为合理。实验以全国地名词典为例,构建5组测试集进行CGQM方法与Lucene检索方法的对比分析。研究结果表明,CGQM方法对于增强地名词典查询功能、提升查询效率具有实际意义。