Establishment and characterization of a testicular Sertoli cell line from olive flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>

The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.     

Impact factors identification of spatial heterogeneity of herbaceous plant diversity on five southern islands of Miaodao Archipelago in North China

Herbaceous plants are widely distributed on islands and where they exhibit spatial heterogeneity. Accurately identifying the impact factors that drive spatial heterogeneity can reveal typical island biodiversity patterns. Five southern islands in the Miaodao Archipelago, North China were studied herein. The spatial distribution of herbaceous plant diversity on these islands was analyzed, and the impact factors and their degree of impact on spatial heterogeneity were identified using CCA ordination and ANOVA. The results reveal 114 herbaceous plant species, belonging to 94 genera from 34 families in the 50 plots sampled. The total species numbers on different islands were significantly positively correlated with island area, and the average α diversity was correlated with human activities, while the β diversity among islands was more affected by island area than mutual distances. Spatial heterogeneity within islands indicated that the diversities were generally high in areas with higher altitude, slope, total nitrogen, total carbon, and canopy density, and lower moisture content, pH, total phosphorus, total potassium, and aspect. Among the environmental factors, pH, canopy density, total K, total P, moisture content, altitude, and slope had significant gross effects, but only canopy density exhibited a significant net effect. Terrain affected diversity by restricting plantation, plantation in turn influenced soil properties and the two together affected diversity. Therefore, plantation was ultimately the fundamental driving factor for spatial heterogeneity in herbaceous plant diversity on the five islands.     

华南埃迪卡拉(震旦)系顶部地层划分及与寒武系界线FAD分子的选择

"雪球事件"以来,早期生命呈现多层次、大规模的加速演化,到寒武纪前夕,生命大爆发达到高潮;在生命剧烈演替的同时,地球环境标志也发生着响应,碳同位素剧烈漂移,并且与各个生命演化节点相对应。但是在划分最重要的地质界线,埃迪卡拉(震旦)系——寒武系界线及界线层型和点位("金钉子",GSSP)确立的问题上迄今仍存在着较大的分歧,以遗迹化石Treptichnus pedum作为该GSSP的标志化石,因其跨系、跨阶产出,生物延限长,因而在全球的生物地层划分对比中(特别在西伯利亚和中国华南地区)一直质疑声不断,而且以遗迹化石作为标志化石本身也具有一定的缺陷。中国华南地区两类古老的宏体化石——矿化管状的Cloudina和条带状的Shaanxilithes在全球范围内可以很好的对比,并且这两种实体化石的出现都对应着碳同位素的明显正漂移。本文通过研究华南震旦系顶部化石带的地层分布和地理分布,综合分析了其时空分布特点和系统演化意义,深入探讨了Cloudina和Shaanxilithes作为寒武系底界标志化石所具备的潜力,对埃迪卡拉(震旦)纪—寒武纪地层界线划分和层型选择提出了更为理想的标准。     

Tectono–Thermal Evolution, Hydrocarbon Filling and Accumulation Phases of the Hari Sag, in the Yingen–Ejinaqi Basin, Inner Mongolia, Northern China

This work restored the erosion thickness of the top surface of each Cretaceous formations penetrated by the typical well in the Hari sag, and simulated the subsidence burial history of this well with software BasinMod. It is firstly pointed out that the tectonic subsidence evolution of the Hari sag since the Cretaceous can be divided into four phases: initial subsidence phase, rapid subsidence phase,uplift and erosion phase, and stable slow subsidence phase. A detailed reconstruction of the tectonothermal evolution and hydrocarbon generation histories of typical well was undertaken using the EASY R_0% model, which is constrained by vitrinite reflectance(R_0) and homogenization temperatures of fluid inclusions. In the rapid subsidence phase, the peak period of hydrocarbon generation was reached at c.a.105.59 Ma with the increasing thermal evolution degree. A concomitant rapid increase in paleotemperatures occurred and reached a maximum geothermal gradient of about 43-45℃/km. The main hydrocarbon generation period ensued around 105.59-80.00 Ma and the greatest buried depth of the Hari sag was reached at c.a. 80.00 Ma, when the maximum paleo-temperature was over 180℃.Subsequently, the sag entered an uplift and erosion phase followed by a stable slow subsidence phase during which the temperature gradient, thermal evolution, and hydrocarbon generation decreased gradually. The hydrocarbon accumulation period was discussed based on homogenization temperatures of inclusions and it is believed that two periods of rapid hydrocarbon accumulation events occurred during the Cretaceous rapid subsidence phase. The first accumulation period observed in the Bayingebi Formation(K_1 b) occurred primarily around 105.59-103.50 Ma with temperatures of 125-150℃. The second accumulation period observed in the Suhongtu Formation(K_1 s) occurred primarily around84.00-80.00 Ma with temperatures of 120-130℃. The second is the major accumulation period, and the accumulation mainly occurred in the Late Cretaceous. The hydrocarbon accumulation process was comprehensively controlled by tectono-thermal evolution and hydrocarbon generation history. During the rapid subsidence phase, the paleo temperature and geothermal gradient increased rapidly and resulted in increasing thermal evolution extending into the peak period of hydrocarbon generation,which is the key reason for hydrocarbon filling and accumulation.     

First Report of Zircon U‐Pb Ages from Lubei Cu‐Ni Sulfide Deposit in East Tianshan of Central Asian Orogenic Belt,NW China

正Objective The East Tianshan mafic-ultramafic rocks belt mainly produced in the eastern Jueluotage belt is an important part of the Central Asia Orogenic Belt(CAOB).The wellknown deposits including Huangshan,Huangshandong,Tulaergen,Hulu,Xiangshan were have been consecutively discovered in this belt(Duan Xingxing et al.,2016).The new discovery of the Lubei Cu-Ni sulfide deposit in recent     

川南地区海相页岩气中发现微量硫化氢

正1研究目的(Objective)自2011年在四川南部地区下古生界海相地层中获得页岩气工业性突破以来,在四川盆地进行的海相页岩气开发得到多方的重视和迅速发展,随之而来的是人们对页岩气勘探开发过程中对环境影响的关注。页岩气,它以吸附或游离状态为主要方式赋存于富有机质泥页岩及其夹层中,是一种非常规天然气。与常规天然气相比,页岩气气质优良,甲烷含量更高。页岩气化学成分主要为甲烷(CH4),一般含量在85%以上,最高达到99.8%,另外还含有少量的乙烷(C2H6)、丙烷(C3H8)和丁烷(C4H10)。一般认为我国页岩气中可以存在少量氮     

“老脚印”对自升式钻井平台插桩滑移的影响研究

渤海海域自升式钻井平台作业频率高,在同一区块多次插桩作业情况多,为自升式平台插桩作业带来严重的滑移风险。通过建立常用的两种类型桩靴的有限元分析模型,采用模拟计算和对比分析的研究方法,对在“老脚印”不同距离位置插桩时,桩基土体的塑性破坏特性、运移规律及桩靴所受侧向滑移力进行了模拟研究,得出了在不同土质条件下,桩基土和桩靴的受力特性及规律,分析了不同位置的“老脚印“对自升式平台插桩滑移风险的影响。     

四川江油马角坝刺林包剖面二叠-三叠系间粘土岩研究

二叠-三叠纪之交华南地区普遍发育火山成因的粘土岩,其对于了解二叠纪末大规模火山活动及扬子板块碎屑物质来源具有重要意义。本文对扬子西缘马角坝刺林包剖面飞仙关组底部粘土岩进行了碎屑锆石U-Pb定年及原位Hf同位素测试、X衍射和全岩地球化学分析,以揭示粘土岩的岩石成分及物源信息。镜下特征、X衍射及主量元素特征表明岩石中粘土矿物主要为伊利石,含少量方解石、石英。碎屑锆石年龄显示,特征峰值年龄主要集中在2 615~2 383、1 868~1 328、1 186~778和430~246 Ma,并出现~250 Ma特征高峰,结合原位Hf同位素特征表明锆石与秦岭造山带、华南典型PTB剖面粘土岩及峨眉山大火成岩省具有较高的相似性。同时,通过微量、稀土元素分析发现粘土岩中Zr、Hf、Th、Cr、Co、Ti相对富集,与华南典型PTB粘土岩和峨眉山玄武岩相近,并结合构造背景及锆石特征,综合认为刺林包剖面PTB粘土岩来自于秦岭造山带及龙门山岛链局部剥蚀区、二叠纪末火山活动和峨眉山大火成岩省剥蚀岩体。     

川东长兴组台缘礁滩相储层纵向非均质性特征及形成机制——以川东宣汉盘龙洞长兴组剖面为例

近几年,随着四川盆地海相碳酸盐岩储层勘探的不断加强,碳酸盐岩储层强的非均质性已成为制约礁滩气藏勘探开发的现实问题。以川东宣汉盘龙洞长兴组典型台缘礁滩剖面为研究对象,通过对典型台缘礁滩野外剖面的精细观察,结合镜下薄片及岩矿资料分析,重点探讨了盘龙洞礁滩储层纵向非均质性特征及成因。剖面上礁滩体垂向上存在很强的旋回性和储层非均质性,其储集岩分布、储层厚度、白云岩化程度、溶蚀作用等受海平面周期性的升降变化影响而呈现周期性变化。礁滩储层非均质性受高能相带、海平面升降变化和建设性成岩作用的共同控制,高能相带控制储层原生孔隙的发育,决定了储层的沉积非均质性,白云岩化和溶蚀作用有利于礁滩体储层的形成,大气淡水淋滤改造对于优化储层具有关键作用,海平面升降变化决定了礁滩体旋回性发育,而建设性成岩作用的选择性改造深化了储层非均质性。