Cloning and sequence analysis of a full-length cDNA of <Emphasis Type="Italic">SmPP1cb</Emphasis> encoding turbot protein phosphatase 1 beta catalytic subunit

Reversible protein phosphorylation, catalyzed by protein kinases and phosphatases, is an important and versatile mechanism by which eukaryotic cells regulate almost all the signaling processes. Protein phosphatase 1 （PP1） is the first and well-characterized member of the protein serine/threonine phosphatase family. In the present study, a full-length cDNA encoding the beta isoform of the catalytic subunit of protein phosphatase l（PPlcb）, was for the first time isolated and sequenced from the skin tissue of flatfish turbot Scophthalmus maximus, designated SmPPlcb, by the rapid amplification of cDNA ends （RACE） technique. The cDNA sequence of SmPPlcb we obtained contains a 984 bp open reading frame （ORF）, flanked by a complete 39 bp 5＇ untranslated region and 462 bp 3＇ untranslated region. The ORF encodes a putative 327 amino acid protein, and the N-terminal section of this protein is highly acidic, Met-Ala-Glu-Gly-Glu-Leu-Asp-Val-Asp, a common feature for PP1 catalytic subunit but absent in protein phosphatase 2B （PP2B）. And its calculated molecular mass is 37 193 Da and pI 5.8. Sequence analysis indicated that, SmPPlcb is extremely conserved in both amino acid and nucleotide acid levels compared with the PPlcb of other vertebrates and invertebrates, and its Kozak motif contained in the 5＇UTR around ATG start codon is GXXAXXGXXATGG, which is different from mammalian in two positions A6 and G3, indicating the possibility of different initiation of translation in turbot, and also the 3＇UTR of SmPPlcb is highly diverse in the sequence similarity and length compared with other animals, especially zebraf＇lsh. The cloning and sequencing of SmPPlcb gene lays a good foundation for the future work on the biological functions of PP1 in the flatfish turbot.     

盐度、pH胁迫对茂名海域文昌鱼磷酸酶活性的影响

实验测定了茂名海域文昌鱼在 96 h 时间间隔内耐受盐度的极限值为:最低 17.24,最高46.24;耐受 pH 的极限值为最低 5.0,最高10.0.通过实验前后文昌鱼体重及酸碱磷酸酶活性的变化对比,找出茂名海域文昌鱼较适合生长的盐度及 pH 范围分别为:28.0～32.0及7.5～8.5.     

茂名地区海雾的微物理结构特征

2007年3月16日至4月29日期间,在广东茂名海洋气象科学实验基地对海雾进行了连续观测,共取得51个雾滴谱样本,在此基础上分析了茂名地区海雾的微物理结构特征。观测分析表明:茂名地区海雾的雾滴谱分布符合Junge分布,平均数密度为57.1个/cm3,平均含水量为0.018 3 g/m3,算术平均直径为4.7μm,算术峰值直径为2.9μm。文中还比较了雾和轻雾情况下雾滴谱分布的不同特征,分析了能见度与含水量之间的关系。     

Estimation and prediction of plastic waste annual input into the sea from China

Marine plastic debris has been a pervasive issue since the last century, and research on its sources and fates plays a vital role in the establishment of mitigation measures. However, data on the quantity of plastic waste that enters the sea on a certain timescale remain largely unavailable in China. Here, we established a model using material flow analysis method based on life cycle assessment to follow plastic product from primary plastic to plastic waste with statistical data and monitoring data from accurate sources. This model can be used to estimate and forecast the annual input of plastic waste into the sea from China until 2020. In 2011, 0.547 3–0.751 5 million tons of plastic waste entered the seas in China, with a growth rate of 4.55% per year until 2017. And the amount will decrease to0.257 1 to 0.353 1 million tons in 2020 under the influence of governmental management. The amount of plastic waste discharged from coastal areas calculated in this study was much larger than that from river, thus it is suggested to strengthen the governance and control of plastic waste in coastal fishery activities in China in order to reduce the amount of marine plastic waste input.     

The use of automated coal petrography in determining maceral group composition and the reflectance of vitrinite

Using photometric and image analysis methods, a reasonable technique and procedure have been developed for the determination of maceral group composition and the reflectance of vitrinite. This procedure is introduced by an optimum method for seeking the interval values of each maceral group, i.e., from the “fingerprint” reflectance histogram, it seeks reasonable interval values and distribution values for vitrinite and other maceral groups. After checking and correction, these values can be put into a program and computerized. This method had advantages such as increased speed of analysis and reproducibility of the results.     

南极树粉孢属真菌胞外多糖的分离纯化及结构表征

研究南极树粉孢属(Oidiodendron truncatum)真菌所产胞外多糖的理化性质和结构特征。采用阴离子交换柱层析和凝胶柱层析对南极树粉孢属(Oidiodendron truncatum)真菌胞外多糖进行了系统的分离纯化,从中得到4个均一的组分(AFW1,AFW2,AFS1和AFS2),并结合红外、气质及核磁等技术对它们的结构进行了解析。结果表明:AFW1和AFW2为中性多糖,AFS1和AFS2中含有少量的蛋白;AFW1和AFS1是由甘露糖、葡萄糖和半乳糖3种单糖组成的结构复杂的杂多糖;AFW2和AFS2则仅由葡萄糖组成,其中AFW2属线性(-1,6-葡聚糖,而AFS2属于C-3位有分支的α-1,6-葡聚糖。     

基于神经网络的滑移隔震结构智能半主动控制

考虑上部结构的刚度和阻尼,使用神经网络控制算法计算基底摩擦力的大小,研究了滑移隔震结构的半主动控制。对计算实例的分析表明,通过半主动控制的滑移隔震结构不但具有较好的隔震效果,且能有效地减小基底的最大滑移量及残余位移。为对比各种控制方法的控制效果,文中还利用Bang-Bang控制和瞬时最优控制算法对滑移隔震结构进行了半主动控制。对比分析表明,基于神经网络控制算法的控制效果优于其它控制算法,具有反馈量少,稳健性强等特点。     

个旧老卡岩体接触-凹陷带构造控矿特征研究

个旧老卡岩体接触-凹陷带是个旧矿集区最重要的锡铜多金属成矿构造带,带内锡铜矿体的成生与凹陷带构造密切相关,受构造、岩性和岩浆活动联合控制,凹陷带的形态、产状、规模的变化直接影响矿体的形态、产状、规模.凹陷带构造是一个有利成矿的构造部位,并配之有其他控矿因素,最终在燕山晚期含矿岩浆气液作用下形成富厚矿体.     

冻土纵、横波宽带超声换能器的研制

根据冻土弹性波测试的要求,研制了一种冻土测试用的５００ＫＨz纵横波复合宽带超声换 能哭喊 。其性能具有灵敏度高、频带宽、声阻撤低的特点,适用于冻土力学特笥的测试。文章介绍了它的结构及相应系统特性,并以１０ｐｓ的延时时间精度,在零度以下气温条件下,实测了不同含水量及负温冻结兰州黄土试块的纵、横波速,获得了好的结果。     

土的边界面本构模型研究进展

简述了土的边界面本构模型国内外研究状况，阐述了其基本思想。对边界面模型作了简要评述，提出了边界面模型研究的发展趋向。