基于高通量测序方法分析荣成月湖潮间带趋磁细菌多样性

荣成月湖是一个典型天然潟湖,潮间带沉积物中存在多种形态趋磁细菌。通过Roche454高通量测序平台,对沉积物样品(B_S)和磁收样品(B_M)进行16SrRNA基因高通量测序,认识荣成潮间带沉积物的细菌群落结构,并了解趋磁细菌多样性及系统进化地位。研究结果表明沉积物样品中主要的细菌类群为δ-变形菌纲,占总细菌数的26.4%,其次是γ-变形菌纲和α-变形菌纲;而磁收样品中细菌多样性和种类明显降低,以α-变形菌纲占绝对优势,相对比例达72.6%。在磁收样品和沉积物样品分别发现了1 612条和186条reads与趋磁细菌相关,分别占细菌总数的5.76%和0.85%,磁收样品中趋磁细菌数是沉积物样品的6.8倍。对两个样品中获得的趋磁细菌序列进行系统进化分析,发现这些序列多数属于变形菌门的α-变形菌纲,以趋磁球菌占绝对优势,少数属于δ-变形菌纲,与多细胞趋磁原核生物亲缘关系最近。海洋趋磁螺菌属仅在B_M样品中检出,趋磁弧菌属在B_M样品优势度高于B_S样品,而多细胞趋磁原核生物和趋磁螺菌属在B_S样品中优势度更高。通过分析样品间的差异OTUs,认为荣成潮间带沉积物中可能存在大量未知的趋磁细菌新类群。研究结果为下一步培养和开发趋磁细菌这一功能菌群,发现趋磁细菌新类群及趋磁细菌生态功能提供了基础资料。     

地理省情监测成果地图集编制探讨

本文通过对《四川省地理省情监测成果地图集》在图集设计思路、地图编制方法等方面的探索,总结出了地理省情监测成果制图表达的基本流程,并以地图形式直观地展示地理省情监测成果。探索了地理国情监测成果图集的设计思路及方法,尤其是动态信息的制图表达方案,为开展地理国情、省情监测等综合地图图集的设计、编制提供借鉴。     

海洋微藻间竞争研究进展

微藻是海洋生态系统中最主要的初级生产者,也是引发赤潮的主要生物[1-2].微藻之间的竞争作用是海洋生态系统的重要组成部分,对竞争机理的探讨,可进一步解释群落结构演替和赤潮发生原因,为赤潮治理提供新的思路,对海洋生态系统的修复和保护具有重要意义,竞争机理在微藻开发利用上的应用,将取得重大的经济效益和社会效益[3].     

Effects of Hypoxia on Dopamine Concentration and the Immune Response of White Shrimp （Litopenaeus vannamei）

Effects of hypoxia on the dopamine concentration and the immune response of White Shrimp Litopenaeus vannamei were studied. The results showed that hypoxia had significant effects on the concentration of dopamine (DA) in the haemolymph, haemocyte count, phenoloxidase activity, phagocytic activity of haemocytes and bacteriolytic and antibacterial activity in the haemolvmph (P<0.05). The concentration of the dopamine in haemolymph reached its maximum in the 3.0 and 1.5mgL-1 DO groups at 12h and 6h, and then returned to normal after 24h and 12h, respectively. All immune parameters decreased with the reduction of dissolved oxygen. Total haemocyte count (THC), the hyaline cells and semi-granular cells in the 3.0mgL-1 DO group became stable after 12h,while granular cells did so after 24h, The THC and different haemocyte count (DHC) in the 1.5mgL-1 DO group became stable alter 24h. Phenoloxidase activity and bacteriolytic activity in the 3.0 and 1.5mgL-1 DO groups reached their stable levels after 24 h and 12 h respectively, while phagocytic activity and antibacterial activity became stable after 24 and 12, and 36 and 24 It, respec-tively. It was also indicated that the changes of dopamine concentrations in haemolymph, haemocyte count and phenoloxidase activ-ity were obviously related to the exposure time under hypoxic conditions.     

基于聚二甲基硅氧烷胶与Zn-Al型水滑石复合膜为离子载体的溶解二氧化碳电极及其性能标定

深海探测溶解CO2气体异常.可以为寻找热液口提供一个很好的证据.本文设计的基于自制的聚二甲基硅氧烷胶与Zn-Al型水滑石复合膜为离子载体的溶解CO2探测电极属于电势型CO2电极,适用于对深海中溶解形式的CO2的测量,具有体积微小、便于与pH、盐度、温度、氧化还原电位等多种传感器集成在同一探头腔内,实施在线探测等优点.实验室条件下.将该溶解CO2探测电极与银,氯化银参比电极分别接在Keithley 6517A高阻抗电位计输入端进行测试,测试结果表明:该电极对不同浓度的碳酸氢钠溶液具有良好的响应信号,响应时间大约在30s-1 min.检测限在10-1-10-4mol·L-1.     

港域波浪数学模型的改进与验证

通过物理模型对改进的港内波浪传播变形数学模型进行验证。该数学模型以推广的时变缓坡方程为控制方程,采用含松弛因子的ADI法求解,并对波浪反射和透射边界模拟方法进行改进。先通过物理模型试验确定斜向浪入射条件下抛石防波堤前的波浪反射系数,作为数学模型中部分反射边界模拟的依据。然后进行了一个典型港口内波浪折射、绕射和反射的模型试验,测量港内波浪分布。对比模型试验和数学模型计算的结果表明,数学模型可较好地模拟港内复杂地形和边界条件下规则波和不规则波的传播变形。     

Sediment texture and grain-size implications: the Changjiang subaqueous delta

Sediment cores were collected from the subaqueous delta of the Changjiang Estuary. Sediment grain-size profiles and their fractal dimensions were analyzed, to elucidate responses to long-term sedimentary processes. In addition, the environmental sensitive populations of grain size have been extracted. The sediment cores can be divided into two parts, according to the sedimentary structures present. The upper part (0-12 cm) is interpreted as being the active layer, which is influenced frequently by changes i...     

The black water around the Changjiang (Yangtze) Estuary in the spring of 2003

The Changjiang (Yangtze) Estuary is located in the East China Sea shelf with shallow water. Affected by the tide mixing and the runoff of the Changjiang River and the Qiantang River, the turbidity is very high. Generally, the water-leaving radiance is high in the turbid water because of the large particle scattering. Based on the in-situ data and ocean color remote sensing data of SeaWiFS, it was found that there was a black water region with the normalized water-leaving radiances less than 0.5 mW/(cm2·μm2·...     

COMCOT数值模式的介绍和应用

介绍了一个成熟的海啸数值模式-COMCOT模式.COMCOT模式是一个能够模拟海啸产生、传播和增水全过程的基于浅水波方程的有限差分模型.模型采用多层网格嵌套,根据海啸在不同区域的传播特点和要求,分别选用不同的分辨率和计算设置,从而兼顾了模式的精度和计算效率.利用此模式模拟了2006年12月26日台湾南部7.2级地震产生海啸波的传播情况,结果与两个潮位站的海啸波监测数据相吻合.     

海底浅表层不同土类静力触探参数与物理力学性质对比研究

海底土的物理、力学特性是海洋地质学、海洋土工学、海洋地质灾害等调查研究及海洋资源开发环境条件调查和评价等方面非常重要的资料,海底静力触探试验(CPT)是获取这方面资料的一种快速高效、成本低、测试精度高的原位测试方法。它可同时获取海底土锥尖阻力、侧摩阻力等方面的连续原位测试数据,这些数据与海底土取样样品测试分析资料及物探资料结合,可对海底土的工程特性等作比较全面的分析和评价。本文对采集的海底土样的静力触探参数与海底土样的物理力学指标进行相关分析,分粘性土和砂类土二类,建立了海底浅表层土的静力触探参数与物理力学指标之间的相关方程。利用相关方程可以通过静力触探的数据来推算土的物理力学指标,进而对海底土的工程情况作较全面的评价。