Effects of cadmium, zinc and nitrogen status on non-protein thiols in the macroalgae Enteromorpha spp. from the Scheldt Estuary (SW Netherlands, Belgium) and Thermaikos Gulf (N Aegean Sea, Greece)

Enteromorpha prolifera (Scheldt Estuary) and E. linza (Thermaikos Gulf) were incubated at three salinities with 100 and 200microgL(-1)Cd and Zn. The objective was to measure effects of Cd, Zn and nitrogen (N) status on the pools of metal-binding non-protein thiols: glutathione and phytochelatins, (gamma-glutamyl-cysteinyl)(n)-glycine (PC). In E. linza, ammonium pools were higher, but amino acid pools, total N and protein contents were lower than in E. prolifera. Reduced glutathione (GSH) pools were positively correlated with free glutamate and protein contents. In E. linza GSH pools increased and the ratio of reduced to oxidized glutathione (GSH:(GSH+0.5GSSG)), an indicator of oxidative stress, decreased with Cd contents, indicating Cd-induced glutathione oxidation. Total glutathione pools (reduced plus oxidized) ranged from 16nmolSgdwt(-1) in controls (at 0.5micromolCdgdwt(-1)) to 179nmolSgdwt(-1) (at 1.9micromolCdgdwt(-1)) at the highest cadmium dosage. Cadmium stimulated PC synthesis in E. prolifera which suggests that in N-rich algae, glutathione pools were high enough for PC synthesis. In both species GSH and protein increased with Zn contents, whereas GSH:(GSH+0.5GSSG) decreased, which would indicate Zn-induced oxidative stress; in E. linza, at the highest salinity the glutathione redox ratio decreased from 0.61 (at 2.9micromolZngdwt(-1)) to 0.26 (at 4.9nmolSgdwt(-1)) (at 0.5molCdgdwt(-1)). PCs were not synthesized in response to Zn, which may have resulted in Zn-induced GSH oxidation. The presence of both oxidative effects (Cd, Zn) and detoxification (Cd) could be identified by observing the responses of glutathione and PC pools to metal stress.     

Effect of Matching Algorithm and Profile Shape on Pulsar Pulse Time of Arrival Uncertainties

For high-precision pulsar timing analysis and low-frequency gravitational wave detection, it is essential to accurately determine pulsar pulse times of arrival(ToAs) and associated uncertainties. To measure the ToAs and their uncertainties, various cross-correlation-based techniques can be employed. We develop methodologies to investigate the impact of the template-matching method, profile shape, signal-to-noise ratio of both template and observation on ToA uncertainties. These methodologies are...     

Effect of Dietary Phosphorus on the Growth and Body Components of Juvenile Synechogobius hasta

The effect of dietary phosphorus on the growth and body components of juvenile Synechogobius hasta was determined. Different percentages of dietary phosphorus (0.63, 0.77, 0.93, 1.06, 1.22 and 1.36) were tested by feeding the fish (body weight, 15.81 g ± 0.32 g; 20 individuals each group; 3 groups each percentage) at a surplus of 5%–10% above satiation for 35 d. Dietary phosphorus did not significantly affect the specific growth rate, feed intake, feed conversion ratio and protein efficiency rate. Nitrogen ...     

Detection of the end point temperature of thermal denatured protein in fish and chicken meat through SDS-PAGE electrophoresis

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied in the detection of the end point temperature (EPT) of thermal denatured protein in fish and meat in this study. It was also used in studying the thermal denatured temperature range of proteins in salmon and chicken meat. The results show that the temperature ranges of denatured proteins were from 65 ℃ to 75 ℃ , and these temperature ranges were influenced by the processing methods. Through SDS-PAGE, the features of repeated he...     

The Effect of Temperature on Growth and Energy Budget of the Polychaete,Neanthes japonica Izuka

Growth and energy budget of the polychaete, Neanthesjaponica, at various temperatures (17, 20, 23, 26 and 29 ℃) were investigated in this study. The growth, as indicated by final dry weight and specific growth rate (SGR), increased with increasing temperature, with the maximum level at 26℃, and then decreased significantly at 29℃. A similar trend was observed in feeding rate, food conversion efficiency (FCE) and apparent digestive rate (ADR). However, no significant differences were detected in ADR among all the temperature treatments. In the pattern of energy allocation, faeces energy was only a small component of energy budget and had little influence on the proportion of food energy allocated to growth. The metabolic energy accounted for a large portion of energy intake for each temperature treatment. The nitrogen excretion was appreciable with changing temperature. The two expendi-ture terms (respiration energy and excretion energy) in energy budget were the major factors influencing the proportion of food en-ergy allocated to growth. These results revealed that temperature affected the growth of N. japonica mainly by influencing feeding rate and FCE. In addition, regression equations describing the relationship between feeding rate, faecal production, SGR, FCE and temperature were obtained. The optimum temperatures for feeding rate, FCE and SGR were estimated at 25.01 ℃, 24.24℃ and 24.73 ℃, respectively, from the regression equations.     

Habitat continuity effects on gradients of fish biomass across marine protected area boundaries

Marine protected areas (MPAs) could be useful as fisheries management tools for the exportation of pelagic eggs, larvae and adult fish. A decreasing gradient of fish biomass across MPAs boundary may indicate export. We determine whether gradients of decreasing biomass of fish assemblage occurred in Tabarca Marine Reserve over two habitats with different continuity across the boundaries, to test if the patchy nature of the marine environment might act as a barrier for the fish export. In general, significant decreasing gradients in total fish biomass and biomass of some species were observed on P. oceanica and rocky substrates, independently of their different continuity through the reserve boundaries. Changes in the multivariate structure of the fish assemblage were correlated with the distance from integral reserve. All of these results support the hypothesis that the exportation of adult fish from Tabarca Marine Reserve occurs, and this process may influence the surrounding fished areas.     

New observations and transit solutions of the inflated exoplanets HAT-P-40b and HAT-P-51b

We present high-precision photometric observations of the transiting exoplanets HAT-P-40 b and HAT-P-51 b by the Rozhen 2-m telescope.The newly-observed transit of HAT-P-40 b is the first one with a complete curve.The orbital periods of the two targets were improved.We modeled the observed transits and found bigger stellar radii than those derived from the stellar models.The planet radii of HATP-40 b and HAT-P-51 b obtained from our transit solutions are bigger than the values calculated by the empirical relations for Jupiter-mass and Saturn-mass planets respectively.Their values reveal the highlyinflated nature of the two targets,especially that of HAT-P-51 b.We established that the best transit solutions correspond to a quadratic limb-darkening law.The fitted limb-darkening coefficients of HAT-P-40 are close to the theoretical ones while those of HAT-P-51 are a little different.The precise astrometric Gaia distances of the two targets are smaller by 6%–7%than the calculated values from the stellar models.We propose the Gaia distances to be used for improvement of the stellar models as well as for more reliable calculation of the parameters of the known exoplanets.     

硫化物饱和的和硫化物未饱合的科马提岩熔融体的化学和热力模拟

科马提岩熔融体的化学的和热力的模拟试验进行了我们对卡姆巴尔达型镍矿床成因的认识。化学模拟的价值明显地受工程使用资料的质量的限制。现有的模式是以Rajamani和Naldrett(1978)提出的镍分配系数的实验测定为根据的。这些系数是在1200℃和1300℃间温度的玄武岩熔融体在未知S及O逸度条件下测定的。如果把这些资料用于模拟科马提熔融体的分馏,就可外推更多超镁铁质成分和超过1500℃温度条件下的结果。     

爱琴地区新构造正断层带的构造非均匀性

一、引言 本文是对爱琴张性构造区新构造正断层带为期五年的野外调查结果所作的综台与分析(图1)．着重介绍爱琴地区的三个地带．(1)希腊大陆的科林斯地区(图1a)．(2)西土耳其的伊密兹尔地区(图1b)．(3)柯雷特的中部及西部(图1c)．在这三个地带正断层使中生代碳酸岩同第四纪沉积物直接相接，