Nitrate assimilation by zooxanthellae maintained in laboratory culture

The ability of cultured zooxanthellae to use ammonium and nitrate was tested. The zooxanthellae were initially isolated from the anemone Aiptasia pulchella. The zooxanthellae were able to grow on media supplemented with either form of dissolved inorganic nitrogen (DIN) although ammonium was inhibitory above 300 μM. In accord with the intact symbiosis and freshly isolated algae, the cultured zooxanthellae took up ammonium. In contrast, the cultured algae demonstrated nitrate uptake and utilization. This was enhanced with DIN deprivation. Nitrate reductase was found in the algae and appeared to be inducible. Within the animal host, excretory ammonium may repress this induction.     

The dissolution of opaline silica of diatom tests in sea water

Two different series of measurements have been made of the solubilities and rates of solution in sea water of acid-washed siliceous tests of the diatomsThalassiosira decipiens andRhizosolenia hebetata. The kinetic approach was carried out on the assumption that the tests behave as identical solid spheres. If the tests were present in considerable excess over that required for saturation of the water with respect to silica, the dissolution obeyed first order reaction kinetics and its rate was proportional to the surface area of the exposed tests. When the tests were not in excess the kinetics of dissolution appeared to be more complex. This was considered to be due to a decrease in specific surface area as dissolution proceeded. An expression developed to allow for the change of surface area as a sphere dissolves offers a partial explanation of the kinetics when the tests are not present in excess.     

Primary production and plankton stocks in the Pacific Ocean and their seasonal variation according to remote sensing and field observations

Data collected from 20 years of observations by the P. P. Shirshov Institute of Oceanology (about 250 comprehensive ecosystem stations) are summarized to evaluate the primary production and biomass of the principal groups of plankton in the Pacific Ocean. The stations were classified into geographical regions according to satellite determined criteria. The areas of these regions were evaluated according to CZCS data from 1978 to 1986. The total value of primary production was evaluated as 26·9 Gt C year⁻¹. Taking the “bottle effect” correction into account, this value may be as high as 45.6 Gt C year⁻¹. Total biomass values of phytoplankton (134 Mt C), bacteria (86 Mt C), protozoa (26 Mt C) and mesoplankton (184 Mt C) were also calculated. Seasonal variabilities of all these values were remarkably insignificant.     

Reversing Water Exchange Patterns at the Entrance to a Semiarid Coastal Lagoon

Water velocity and density profiles were obtained in late-spring and late-winter to document reversing mean circulation patterns at the entrance to a semiarid coastal lagoon, the Bay of Guaymas, in the Gulf of California, Mexico. The lagoon is shallow but the bathymetry at its entrance is similar to that of temperate estuaries with an asymmetrically positioned channel flanked by shoals. In late-spring the mean circulation at the entrance to the lagoon was driven by horizontal density gradients that arose from excess evaporation over precipitation in the area as evidenced by water density profiles. The lagoon exported relatively warm (25·8 °C) and salty (36·2) water to the Gulf of California through the channel. This export was consistent with inverse estuarine circulation influenced by bathymetry. In late-winter, the circulation at the entrance of the lagoon was mostly driven by wind stress that blew from the northwest, roughly along the main axis of the lagoon. Relatively cool (16·0) °C) and less salty (35·1) water from the Gulf of California was driven into the lagoon within the channel. Density gradients inside the lagoon seem to have played a secondary role in driving the circulation. The late-winter circulation was then estuarine-like, with outflow in the direction of the wind over the shallow areas and a compensatory inflow appearing in the channel as expected from theory of wind-driven flow over bathymetry. This estuarine-like circulation developed despite the lack of measurable freshwater input to the lagoon and was the opposite to that observed in late-spring. These observations then document a reversal in water exchange patterns from season to season in a semiarid coastal lagoon. The observations also constitute one of the few reported examples of flow over shoals driven in the same direction as the wind stress with a compensatory flow in the channel.     

Microbiological and chitinoclastic activities associated withPenaeus setiferus

Analysis of microbiological and chitinase activities relative to the white shrimpPenaeus setiferus, and its chitinoclastic endosymbionts, have demonstrated the relevance of total chitinolytic processes in penaeid biology. Microorganisms may serve as a direct source of nutrients for the animal as well as in the elaboration of extracellularin situ chitinase enzymes. The enzyme produced by the predominant gut bacteria,Beneckea neptuna, is a moderately actively inducible chitinase while the shrimp has an indigenous constitutive chitinase and chitobiase system. Factors of temperature, pH, ion inhibition and reducing sugar ratios have been compared for the bacterial and animal enzymes. This dual enzyme system suggests that metabolic chitin transformation may play a vital role in crustacean metabolism.     

Prolonged Magmatic and Tectonic Development of the Caribbean Igneous Province Revealed by a Diving Submersible Survey

Using the diving submersible survey NAUTICA we investigated the central part of the Caribbean large igneous province (CLIP) to observe and sample internal portions of this proposed oceanic plateau. Most of the samples are gabbroic and doleritic rocks; basalts are scarce. Radiometric dating by ⁴⁰Ar/³⁹Ar incremental heating experiments indicate that the intrusive rocks are Campanian in age (81–75 Ma). In some places these intrusive rocks underlie older Santonian (85–83 Ma) extrusive basaltic rocks, suggesting that the Campanian rocks represent a sill injection and an underplating episode. Results of the diving program supplemented by information from ODP and DSDP drilling sites document a 20 m.y. period (94–75 Ma) of igneous activity in the submerged portion of the Caribbean large igneous province (CLIP). In the northern part of the Beata Ridge late Campanian and/or post Campanian uplift is documented by prominent Maastrichtian (71–65 Ma) erosion and the establishment of a Paleocene-middle Eocene (65–49 Ma) carbonate platform. During and after the uplift an extensional period is indicated by seismic images and the subsidence (3 km depth) of the carbonate platform. Paleocene ages (55–56 Ma) determined on some volcanic samples are attributed to localised decompression mantle melting that accompanied the extension. We document a prolonged period of magmatic and tectonic events that do not fit with the current models of short-lived plateau formation during mantle plume initiation but shares many similarities with the constructional histories of other oceanic large igneous provinces.     

Formation of pockmarks by pore-water escape

During the course of a North Sea rig site investigation, a number of seabed depressions were observed on side-scan sonar records, some of which may be identified as pockmarks. Others are described as pits. A pockmark evolutionary series is proposed on the basis of all these features with a suggested mechanism which would favor release of pore water, but does not exclude gas escape. Acoustic voids are considered to be a related phenomenon.     

Surface heat fluxes and subsurface heat content at a site over the southeastern Bering Sea shelf, May–July 1996

Observations from a surface mooring, in a weak-flow regime over the southeastern Bering Sea shelf, were used to derive surface heat fluxes for the period May–July 1996. Changes in heat content of the water column also were determined from subsurface temperature measurements. Agreement of net surface heat flux and change in heat content was within 2%. This result provides additional evidence that heat advection and diffusion are small in this region.     

The dynamic method of calculating currents and analysis of the vertical kinematic structure

The basic relations of the classical dyamic method have been complemented with the condition allowing for the non-monotonicity of the vertical kinematic structure. The elements of the multi-layered system of geostrophic currents have been found to correlate over depth with seawater density and hydrostatic pressure distribution. An example of the analysis of the vertical structure of currents, involving specific hydrological data, is given. Translated by Vladimir A. Puchkin.