利用RAPD和AFLP标记初步构建太平洋牡蛎的遗传连锁图谱

利用RAPD和AFLP标记 ,以一回交家系 [(Miyagi×Hiroshima)×Miyagi]为作图群体 ,构建了太平洋牡蛎的遗传连锁图谱。用经过筛选的 3 3个RAPD引物和 1 1个AFLP引物组合 ,对父母本和 80个子代个体进行了遗传分析。共得到母本分离标记 1 93个 ,其中 1 44个符合1∶1孟德尔分离规律。父本分离标记 1 5 6个 ,其中 1 1 1个符合 1∶1孟德尔分离规律。雌性框架图包括 99个遗传标记 ,定位在 1 2个连锁群中 ,覆盖 985 2cM ,标记间平均间隔 1 1 3cM。另外有 3个三联体 ,7个连锁对 ,图谱共覆盖 1 1 6 5 7cM。雄性框架图包括 72个遗传标记 ,分布在 8个连锁群 ,覆盖 81 1cM ,标记间平均间隔 1 2 7cM。另外有 4个三联体 ,3个连锁对 ,图谱共覆盖93 1 8cM。     

Diagnosis of iridovirus in large yellow croaker by PCR

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10^-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.     

Nature and composition of shore-zone sediments between Jeddah and Yanbu, eastern Red Sea

The shore-zone sediments between Jeddah and Yanbu, west coast of Saudi Arabia, are composed mostly of skeletal carbonate sands. The nearshore sediments containing benthic foraminifera, algal fragments and molluscs are multimodal, the mean grain size varying between 0.76 and 2.35 ø. The beach sediments, except samples dominated by cerithid gastropods in some localities, are relatively finer than the nearshore sediments. Although the beaches to a great extent comprise sand-sized material, fine lime muds and coarse clastic gravels occur in certain areas. The dune sediments comprising mostly algal grains and ooids are very fine with 50 percent of the material in the 0.25–0.18 mm size grade. Except a general northward decrease in mean grain size, regional trends in the textural parameters of the sediments between Jeddah and Yanbu are not quite apparent. Lateral variations in the textural characteristics suggest a landward migration of the sediments in the shore zone under the influence of northerly and northwesterly winds. The carbon and oxygen (δ ¹³C + 4.80 to 4.84‰ PDB) (δ ¹³O − 0.04 to + 0.53‰ PDB) isotopic ratios of the lime muds occurring in certain shallow margins in the shore-zone, which are much higher than those of the green algae, indicate that the fine carbonates are at least in part inorganic in origin.

Like the shallow-water carbonates in tropical seas, aragonite and high Mg-calcite are the dominant carbonate minerals in the shore-zone sediments. There is a landward increase in aragonite contents caused by the landward migration of fine material from the nearshore. The dominant clay mineral in the nearshore sediments is kaolinite with subordinate swelling chlorite and little illite. Kaolinite is contributed by the coastal regions under the sub-tropical humid climate. Swelling chlorite is considered to have been formed in the nearshore by mechanical mixture of chlorite and montmorillonite derived from the metamorphic and igneous terrains of the Tertiary mountains bordering the coastal plain.     

In-Situ Observation of Fluid Mud in the North Passage of Yangtze Estuary, China

Observations of fluid mud were made in the lower North Passage of the Yangtze Estuary in February 2000, on 10 -11 August 2000, on 30 - 31 August 2000 (after two strong typhoons), on 21 - 24 August 2000 (neap tide) and on 3 -6 September 2000 (mean tide) respectively. In situ data show that the fluid mud in this area consists of fine cohesive sediment (median size 7.23 μm). The formation and movement of fluid mud varied during the neap-spring and flood-ebb tidal cycle. Observations suggest that fluid mud phenomena in this area may be categorised in a three-fold manner as slack water, storm and saltwedge features. The thickness of the fluid mud layer of slack water during the neap tide ranged from 0.2 to 0.96 m, whereas during the mean tide, the thickness ranged from 0.17 to 0.73 m, and the thickness of the fluid mud layer was larger during slack water than at the flood peak. Shoals cover an area of 800 km^2 with a water depth smaller than 5 m. Erosion of these extensive intertidal mudflats due to storm action provides an abundant sediment source. This is particularly significant in this estuary when the tidal level is lower than 5 m. The lower North Passage is a typical zone of saltwater wedging, so the saltwedge fluid mud has the most extensive spatial range in the estuary.     

Model estimates for the mean gas exchange between the ocean and the atmosphere under the conditions of the present-day climate and its changes expected in the 21st century

Annual mean fluxes of CO₂ and oxygen across the sea surface are estimated with the use of numerical modeling for several regions located in the Gulf Stream and Kuroshio zones. The present-day climatic conditions and the climatic conditions expected in the middle and at the end of the 21st century are considered. Specific features of gas exchange under a strong wind that are associated with gas exchange by bubbles and with changes in the air-water difference of the gas concentrations were taken into account in the calculations. The estimates obtained differ substantially from the results based on the traditional approach, which disregards the above features. A considerable increase in the absorption of CO₂ by the ocean, which is mainly caused by the continuing increase in the CO₂ concentration in the air during its small changes in the ocean, is expected in the 21st century. At the same time, no trends are revealed in the annual mean fluxes of oxygen across the ocean surface. The conclusion is made that, in calculations of CO₂ absorption by the world ocean, it is necessary to take into account both specific features of gas transfer under a strong wind and an increase in the atmospheric concentration of CO₂.     

Basement structure and sedimentary cover seismostratigraphy in the northern part of the Japan Basin in the region of the Tarasov Rise (Sea of Japan)

The results of continuous seismic profiling thermodynamics performed in the northern part of the Japan Basin in the region of the Tarasov Rise and the data of a micropaleontological examination of the diatom remains encountered in the sediment samples from the rise and continental slope are presented. In the area studied, the topography of the acoustic basement features a vast rise (plateau) buried under the sedimentary cover outlined by the depth contour 5.8 s. The plateau has a relatively smooth top surface crossed by a series of rises of the acoustic basement. The two largest rises are represented by the ridges of the Tarasov Rise. The plateau is separated from the continental slope by a depression in the acoustic basement with a depth up to 6.8 s. From the end of the Middle Miocene up to the beginning of the Paleocene, the region of the plateau represented an area of active volcanism; it coincided in time with the stage of subsidence of the floor of the acoustic basement depression. At the end of the Late Miocene, the ridges of the Tarasov Rise started to sink. In the Pliocene, this process accelerated, and, at the beginning of the Pleistocene, it stopped. In the Middle Miocene-Early Pleistocene time, the portion of the continental slope adjacent to the plateau remained stable and suffered no significant vertical movements.     

Major variability modes and wind patterns over the Sea of Japan and adjacent areas

The expansion of wind fields observed at fixed times (four times daily) in complex empirical orthogonal functions is performed for the Japan Sea area (34°–53° N, 127°–143° E). The wind fields are taken from the 1998–2004 NCEP/NCAR Reanalysis data with better spatial resolution (1° × 1°) than the standard product, which are publicly available on the Internet. Major modes of wind variability in the Japan Sea area are identified. The modes determine a general direction of air-mass transport throughout a year, zonal and meridional modulation, and a cyclonic and an anticyclonic eddy component. Objective classification of wind fields with respect to the prevailing flow direction is performed, and wind stress and wind-curl patterns are obtained for major events in the cold and warm periods of the year. The pattern obtained can be used in hydrodynamic numerical models of the general circulation of the Japan Sea.     

Sediment transport pattern at the Barra Nova inlet, south Portugal: a conceptual model

The Barra Nova inlet, in south Portugal, is known to migrate progressively southeastwards under wave action. The morphodynamics of this system during a representative year suggests that this long-term evolution is dependent on a seasonal behavior of the tidal inlet which can be described through a three-stage model of post-storm, transition and extended fair-weather conditions. Processes involved in this evolution indicate that the historical migration of the Barra Nova is not dependent on the longshore drift forcing constricting the channel on the updrift coast, but rather on the adjustment of the system to a major erosion of the downdrift coast during short storm events.     

耳鲍(Haliotis asinina)核糖体小亚基(18S rRNA)编码基因的克隆与序列分析

采用分子生物学的方法,对南海不同海区的两个地理群体耳鲍(Haliotis asinina)18S rRNA基因全长进行了克隆和序列分析,并将耳鲍18S rRNA基因的序列与NCBI数据库中已收录鲍的18SrRNA基因进行了比较。结果发现,南海耳鲍核糖体18S rRNA基因与耳鲍H.asinina isolate H11核糖体18S rRNA基因序列的相同率高达98%;同一地理群体内耳鲍核糖体18S rRNA基因序列完全一致;不同地理群体间耳鲍核糖体18S rRNA基因在碱基组成上的相似率为99%,仅在某些位点处发生了碱基替换,即腺嘌呤(T)被鸟嘌呤(G)替换;同时,将这两个不同群体中耳鲍的18S rRNA基因与泰国耳鲍18S rRNA基因序列进行比较分析发现,它们之间也只是发生了碱基替换。     

棉粕蛋白酶解物对异育银鲫(Carassius auratus gibelio)消化、生长和胰蛋白酶mRNA表达量的影响

以棉粕蛋白为酶解底物,用枯草杆菌蛋白酶对其进行酶解,以酶解产物1.5%和3.0%两个梯度等量替代鱼饲料配方中棉粕,在室内流水养殖系统中喂养异育银鲫鱼种[体重为(30±2)g]65天。测定鱼的生长、营养物质表观消化率、消化蛋白酶活性及肝胰脏中胰蛋白酶mRNA表达水平等指标。结果表明,添加1.5%和3.0%棉粕酶解产物的鱼在饲养35天后的特定增长率(SGR)分别比对照组高32.5%和56.7%,且差异显著(P<0.05);在饲养65天后,两组特定增长率分别比对照组高8.0%和21.0%,且差异极显著(P<0.01),肝胰脏中胰蛋白酶mRNA表达水平也随棉粕酶解产物添加梯度提高而相应提高,表明鱼的生长与消化蛋白酶mRNA表达水平相关。同时棉粕蛋白酶解物对肠道蛋白酶的活性和营养物质表观消化率都有促进作用,而棉粕蛋白酶解物对鱼肌肉成分并没有改变,这也表明棉粕蛋白酶解物在促进鱼生长、内源酶活性同时并未降低鱼的品质。