Can light intensity cause shifts in natural product and bacterial profiles of the sponge Aplysina aerophoba?

The potential role of microbial consortia on sponge chemistry is well known. However, how environmental factors affect microbial and chemical profiles and how these shifts affect the sponge holobiont are far from being understood. This study experimentally investigated the effect of light on both the concentration of secondary metabolites and the bacterial assemblages of the sponge Aplysina aerophoba. We quantified major brominated alkaloids (BAs) using a high‐performance liquid chromatography system coupled with a UV‐detector and analysed sponge‐associated bacteria using denaturing gradient gel electrophoresis of 16S rRNA gene amplicons. We identified distinct chemical and bacterial profiles between the ectosome and the choanosome of A. aerophoba. The abundance of most secondary metabolites increased regardless of the illumination regime. We found that the probability of occurrence of three microbial phylotypes (operational taxonomic units 84, 86 and 87) was strongly associated with increasing concentrations of three brominated compounds (aerophobin‐1, aplysinamisin‐1 and isofistularin‐3). Although the role of these bacteria remains uninvestigated, these associations between natural products and specific microbial phylotypes outline further hypotheses that will improve our understanding of the organization and functioning of these complex host–symbiont interactions.     

Lithological control of land subsidence induced by groundwater withdrawal in new urban AREAS (Granada Basin,SE Spain). Multiband DInSAR monitoring

Ground subsidence in the southeastern border of the Granada Basin (SE Spain) has been studied using remote sensing techniques. Over the last decades, the region has experienced a huge urban expansion, which has caused a substantial increase in water supply requirements. Water needs are exclusively met by groundwater by means of numerous pumping wells, which exploit a confined detrital aquifer of alluvial fan deposits with a heterogeneous facies distribution. A general piezometric level decline (up to 50 m) has been recorded in the aquifer during the past 30 years that has induced the generation of a subsiding area with oval shape oriented WNW‐ESE just where the new urban areas and pumping wells are located. Subsidence has been monitored by exploiting synthetic aperture radar (SAR) images from ENVISAT (2003–2009) and Cosmo‐SkyMed (2011–2014). A new approach, which combines A‐DInSAR and small‐area persistent scatterer interferometry (PSI) analysis, has been applied obtaining a good accuracy regarding temporal and spatial dimension of the subsidence. ENVISAT data (2003–2009) reveal subsidence rates up to 10–15 mm/year, and Cosmo‐SkyMed (2011–2014) values slightly lower; up to 10 mm/year. Temporal variations in the subsidence velocity are in accordance with the rainfall pattern and piezometric fluctuations in the aquifer. The sector with highest rates of subsidence does not correspond to the area with more intense groundwater exploitation but to the area with greater presence of clays in the confining layer of the aquifer. There is a clear lithological control in the spatial distribution of the ground subsidence. This work integrates detailed geological and hydrogeological data with differential SAR interferometry monitoring with the aim to better understand subsidence processes in detrital aquifers with small‐scale heterogeneity. Copyright © 2016 John Wiley & Sons, Ltd.     

Syntectonic unconformities of the Alto Cardener,Spanish Pyrenees: a genetic interpretation

The age of three syntectonic unconformities belonging to the Upper Eocene—Middle Oligocene is discussed. The structural characteristics of the conglomerates in the Catalonian south Pyrenees are described with special attention to mechanisms of tectonics and sedimentation and to the lateral relationships between conformity, progressive unconformity (or cumulative wedge system) and angular unconformity. The older unconformities have been tectonically deformed. A new genetic model is proposed to explain offlaponlap mechanisms of each folding phase and the development of angular and progressive unconformities.     

Influence of water mixing on the inhibitory effect of UV radiation on primary and bacterial production in Mediterranean coastal water

The scaling of the solar ultraviolet radiation (UVR, 280–400 nm) effect on phyto- and bacterio-plankton at the ecosystem level is difficult since its estimate is often based on short-time incubation experiments performed at fixed depths, neglecting the previous days’ radiation history and the variable radiation caused by vertical mixing. To examine this issue, we measured primary (PP) and bacterial (BP) production in samples from coastal water in the Northwest Mediterranean Sea incubated at fixed depths or moving vertically within the water column (0–8 m) with a periodicity of 22 min, exposed to full sun, PAR or maintained in dark. Three experiments were carried out on consecutive days to measure day-to-day variations in planktonic response. In surface waters, PP was inhibited by ~32 to 42% by UVR, and BP was inhibited by ~50 to 70% by solar radiation (UVR + PAR). We observed a general decrease in the integrated inhibition of PP due to UVR for both fixed and moving incubations over the 3 days from ~27% of inhibition to non-significant inhibition. In contrast, large discrepancies were observed in the integrated inhibition of BP due to solar radiation (UVR + PAR) between fixed and moving incubations. Whereas both type of incubations gave similar estimation of solar radiation inhibition on day 1 (~25%), inhibition became much higher for fixed incubation compared to moving incubation on days 2 and 3. Differences in responses between days suggest that light history, spectral quality, photoadaptation or acclimation may be important factors in daily observed responses. Our results also underline, for the first time, the importance of the vertical mixing in the BP inhibition by solar radiation.     

Impact of chemical and meteorological boundary and initial conditions on air quality modeling: WRF-Chem sensitivity evaluation for a European domain

This study evaluates the impact of different chemical and meteorological boundary and initial conditions on the state-of-the-art Weather Research and Forecasting (WRF) model with its chemistry extension (WRF-Chem). The evaluation is done for July 2005 with 50 km horizontal resolution. The effect of monthly mean chemical boundary conditions derived from the chemical transport model LMDZ-INCA on WRF-Chem is evaluated against the effect of the preset idealized profiles. Likewise, the impact of different meteorological initial and boundary conditions (GFS and Reanalysis II) on the model is evaluated. Pearson correlation coefficient between these different runs range from 0.96 to 1.00. Exceptions exists for chemical boundary conditions on ozone and for meteorological boundary conditions on PM₁₀, where coefficients of 0.90 were obtained. Best results were achieved with boundary and initial conditions from LMDZ-INCA and GFS. Overall, the European simulations show encouraging results for observed air pollutant, with ozone being the most and PM₁₀ being the least satisfying.     

Integrated geophysical and morphostratigraphic approach to investigate a coseismic (?) translational slide responsible for the destruction of the Montclús village (Spanish Pyrenees)

A 12 million of m³ translational rockslide developed on a dip slope underlain by limestone with interlayered marls, and responsible for the destruction of the Montclús village in the fourteenth century, has been investigated by means of geomorphological and geophysical surveys. The combination of historical-geoarcheological, geomorphological, seismic refraction and electrical resistivity imaging datasets allowed the (1) reconstruction of the late Quaternary episodic evolution of the landslide, (2) characterization of the geometry and internal structure of the slid mass and (3) identification of preferential groundwater flow paths that favoured slope instability. The development of the landslide involved at least two different displacement episodes controlled by sliding surfaces at successively deeper stratigraphic positions. The first landsliding event, recorded by highly weathered landslide deposits situated above a perched failure plane, occurred approximately during the global Last Glacial Maximum (23–19 ka BP). The most recent event, which destroyed the Montclús village built on already slid rocks, is placed in the fourteenth century. Most probably, this reactivation event was triggered by the 1373 Ribagorza earthquake, with an estimated moment magnitude of M _w 6.2. This work illustrates the benefits of combining geomorphological data with complementary geophysical technics in landslide investigation.     

GPR survey to confirm the location of ancient structures under the Valencian Cathedral (Spain)

This paper describes the ground-penetrating radar (GPR) survey performed inside the Cathedral of Valencia, Spain. It is part of historical studies performed in the Cathedral in order to add information to old maps and documents in the Cathedral Archives and also to analyze the extent and importance of potentially destructive moisture areas that were appearing on the floor. The construction of the Cathedral of Valencia occurred in three stages, all of which are well-documented in the Cathedral Archives with detailed drawings, maps, and charts. The radar data were successful in locating crypts, ossuaries, sepulchers, and graves, and the location of ancient walls that existed before the final Cathedral expansion. Three cultural layers corresponding to the three periods of construction were also identified corresponding to the Roman, Arabian and Middle Age Epochs. Measurements of relative sub-floor moisture were obtained by comparing dielectric permittivity changes and radar velocity differences between materials in humid and non-humid areas.     

Application of Multiple Point Geostatistics to Non-stationary Images

Simulation of flow and solute transport through aquifers or oil reservoirs requires a precise representation of subsurface heterogeneity that can be achieved by stochastic simulation approaches. Traditional geostatistical methods based on variograms, such as truncated Gaussian simulation or sequential indicator simulation, may fail to generate the complex, curvilinear, continuous and interconnected facies distributions that are often encountered in real geological media, due to their reliance on two-point statistics. Multiple Point Geostatistics (MPG) overcomes this constraint by using more complex point configurations whose statistics are retrieved from training images. Obtaining representative statistics requires stationary training images, but geological understanding often suggests a priori facies variability patterns. This research aims at extending MPG to non-stationary facies distributions. The proposed method subdivides the training images into different areas. The statistics for each area are stored in separate frequency search trees. Several training images are used to ensure that the obtained statistics are representative. The facies probability distribution for each cell during simulation is calculated by weighting the probabilities from the frequency trees. The method is tested on two different object-based training image sets. Results show that non-stationary training images can be used to generate suitable non-stationary facies distributions.     

Alkenones and coccoliths in ice‐rafted debris during the Last Glacial Maximum in the North Atlantic: implications for the use of UK37′ as a sea surface temperature proxy

The U^K₃₇′ index has proven to be a robust proxy to estimate past sea surface temperatures (SSTs) over a range of time scales, but like any other proxy, it has uncertainties. For instance, in reconstructions of the Last Glacial Maximum (LGM) in the northern North Atlantic, U^K₃₇′ indicates higher temperatures than those derived from foraminiferal proxies. Here we evaluate whether such warm glacial estimates are caused by the advection of reworked alkenones in ice‐rafted debris (IRD) to deep‐sea sediments. We have quantified both coccolith assemblages and alkenones in sediments from glaciogenic debris flows in the continental margins of the northern North Atlantic, and from a deep‐sea core from the Reykjanes Ridge. Certain debris flow deposits in the North Atlantic were generated by the presence of massive ice‐sheets in the past, and their associated ice streams. Such deposits are composed of the same materials that were present in the IRD at the time they were generated. We conclude that ice rafting from some locations was a transport pathway to the deep sea floor of reworked alkenones and pre‐Quaternary coccolith species during glacial stages, but that not all of the IRD contained alkenones, even when reworked coccoliths were present. We speculate that the ratio of reworked coccoliths to alkenone concentration might be useful to infer whether significant reworked alkenone inputs from IRD did occur at a particular site in the glacial North Atlantic. We also observe that alkenones in some of the debris flows contain a colder signal than estimated for LGM sediments in the northern North Atlantic. This is also clear in the deep‐sea core studied where the warmest intervals do not correspond to the intervals with large inputs of reworked coccoliths or IRD. We conclude that any possible bias to U^K₃₇′ estimates associated with reworked alkenones is not necessarily towards higher values, and that the high SST anomalies for the LGM are unlikely to be the result of a bias caused by IRD inputs. Copyright © 2011 John Wiley & Sons, Ltd.