超临界CO2流体萃取土壤中污染物的应用研究进展

CO2在超临界状态下具有许多独特的物理化学性质,作为一种“绿色”溶剂,它具有无毒、无污染、易获取等优点。近年来,有关超临界CO2流体热力学相行为和溶解度性质的研究引起人们广泛的关注,与之密切相关的超临界流体萃取技术已在许多领域得到重视和发展。根据近年国内外运用超临界CO2流体对土壤中多环芳烃、多氯联苯、重金属等污染物的萃取应用,分析了温度、压力、共溶剂等因素对萃取效果的影响,通过与其他土壤修复技术的比较,展望了超临界流体萃取技术应用于土壤修复领域的可行性和研究方向。     

FIO-ESM v2.0 CORE2-forced experiment for the CMIP6 Ocean Model Intercomparison Project

We introduced the Coupled Model Intercomparison Project Phase 6 (CMIP6) Ocean Model Intercomparison Project CORE2-forced (OMIP-1) experiment by using the First Institute of Oceanography Earth System Model version 2.0 (FIO-ESM v2.0), and comprehensively evaluated the simulation results. Unlike other OMIP models, FIO-ESM v2.0 includes a coupled ocean surface wave component model that takes into account non-breaking surface wave-induced vertical mixing in the ocean and effect of surface wave Stokes drift on air-sea momentum and heat fluxes in the climate system. A sub-layer sea surface temperature (SST) diurnal cycle parameterization was also employed to take into account effect of SST diurnal cycle on air-sea heat ?uxes to improve simulations of air-sea interactions. Evaluations show that mean values and long-term trends of significant wave height were adequately reproduced in the FIO-ESM v2.0 OMIP-1 simulations, and there is a reasonable fit between the SST diurnal cycle obtained from in situ observations and that parameterized by FIO-ESM v2.0. Evaluations of model drift, temperature, salinity, mixed layer depth, and the Atlantic Meridional Overturning Circulation show that the model performs well in the FIO-ESM v2.0 OMIP-1 simulation. However, the summer sea ice extent of the Arctic and Antarctic is underestimated.     

香港牡蛎(Crassostrea hongkongensis)新型凝集素ChPerlucin的基因克隆与功能研究

C-型凝集素(C-type lectin)是一类能识别并结合多糖的免疫蛋白质超家族, 本文首次克隆了一种新型香港牡蛎C-型凝集素基因——甘露聚糖结合凝集素, 并获得了该基因的全长序列, 命名为ChPerlucin。结果显示, ChPerlucin基因cDNA全长577bp, 包括21bp的5′末端非翻译区(untranslated region, UTR)、73bp的 3′UTR, 以及483bp的开放阅读框(open reading frame, ORF), 编码160个氨基酸, ChPerlucin蛋白理论分子量为18kD, 等电点为5.95。ChPerlucin蛋白含有保守的C-型凝集素样结构域(C-type lectin or carbohydrate-recognition domain, CLECT); 邻接法(Neighbor-Joining, NJ)进化树分析表明, ChPerlucin与其他贝类的Perlucin聚为一支, 说明该基因是软体动物Perlucin家族的新成员。qRT-PCR结果显示, ChPerlucin在香港牡蛎所检测组织和各个胚胎发育时期中均有表达; 病原菌刺激后, ChPerlucin表达量显著升高; 利用毕赤酵母真核成功表达了ChPerlucin重组蛋白, 发现ChPerlucin重组蛋白具有抑菌效果。以上结果表明ChPerlucin在香港牡蛎的先天性免疫过程中发挥了重要的作用。     

海洋重力仪稳定性测试与零点漂移问题

稳定性指标是海洋重力仪最重要的技术特性之一。针对国内对海洋重力仪稳定性测试与评估重视不够、数据处理过程欠规范、技术指标要求欠细化等现实问题,研究探讨了海洋重力仪稳定性测评的技术流程和数据处理方法,重点分析了环境因素和重力固体潮效应对测试结果的影响,提出了重力仪零点趋势性漂移、有色观测噪声与随机误差的分离方法,建立了比较完善的海洋重力仪稳定性评估指标体系,分析论证并进一步明确了重力仪零点漂移非线性变化的限定指标要求,为修订现行海洋重力测量作业标准提供了可靠的理论依据。     

《海道测量规范》修订的若干问题

根据15年来GB12327-1998《海道测量规范》的使用情况和海洋测绘新技术的发展,针对《海道测量规范》的指导思想、框架结构、技术方法、技术指标等方面进行了探讨,提出了相应的修订建议,以进一步增强规范的先进性、科学性、权威性。     

机载激光测深系统研制中的关键技术

根据机载激光测深系统的工作机理,分析梳理了研制过程中涉及的主要关键技术,包括:高重复频率激光器研制、大动态范围微弱光信号提取、海底回波识别、海面波浪改正、浅水与深水分离测量、高速多通道数字采集、海陆分界识别、定位测姿、水位改正和测深数据质量控制等技术。只有成功突破上述关键技术,才能实现《海道测量规范》要求的测深精度指标。     

龙须菜RSAP分析及其SCAR标记的转化

初步探讨限制性位点扩增多态性(Restriction site amplified polymorphism,RSAP)分子标记技术在龙须菜遗传多样性检测和种质鉴定上的应用。利用所优化的适合于龙须菜RSAP分析的PCR反应体系,对青岛湛山湾野生群体和栽培品系981、07-2进行遗传多样性分析和比较。试验采用15对引物组合在3个群体14个龙须菜个体中共扩增出669个位点,其中多态位点数为146个,多态性比例22%,平均每对引物产生10条多态性条带,片段大小在100~1 000 bp之间。湛山湾野生群体的Na(1.007 5)、Ne(1.007 1)、H(0.003 6)、I(0.005 1)与栽培品系981的Na(1.003 0)、Ne(1.002 1)、H(0.001 2)、I(0.001 8),以及栽培品系07-2的Na(1.009 0)、Ne(1.006 3)、H(0.003 7)、I(0.005 4)相比较可知3个群体的遗传多样性是有差异的。种群间的遗传多样性分析表明,在所有检测的样品中,遗传多样性多来自不同群体间的多样性。群体遗传结构分析表明,2个栽培品系981、07-2间遗传距离不大,但栽培品系与湛山湾野生群体间的遗传距离相对较大,而UPGMA聚类分析也明显的将湛山湾野生群体与栽培品系981、07-2区分开来,表明野生群体与栽培品系间已产生一定的遗传隔离。通过分析湛山湾野生群体及栽培品系981、07-2的RSAP指纹图谱,从中筛选栽培品系981的特异条带,并将其转化为稳定性好、特异性高的序列特征化扩增区(Sequence characterized amplified regions,SCAR)分子标记。     

Effects of meridional sea surface temperature changes on stratospheric temperature and circulation

Using a state-of-the-art chemistry-climate model,we analyzed the atmospheric responses to increases in sea surface temperature （SST）.The results showed that increases in SST and the SST meridional gradient could intensify the subtropical westerly jets and significantly weaken the northern polar vortex.In the model runs,global uniform SST increases produced a more significant impact on the southern stratosphere than the northern stratosphere,while SST gradient increases produced a more significant impact on the northern stratosphere.The asymmetric responses of the northern and southern polar stratosphere to SST meridional gradient changes were found to be mainly due to different wave properties and transmissions in the northern and southern atmosphere.Although SST increases may give rise to stronger waves,the results showed that the effect of SST increases on the vertical propagation of tropospheric waves into the stratosphere will vary with height and latitude and be sensitive to SST meridional gradient changes.Both uniform and non-uniform SST increases accelerated the large-scale Brewer-Dobson circulation （BDC）,but the gradient increases of SST between 60°S and 60°N resulted in younger mean age-of-air in the stratosphere and a larger increase in tropical upwelling,with a much higher tropopause than from a global uniform 1.0 K SST increase.     

A multi-scale urban atmospheric dispersion model for emergency management

To assist emergency management planning and prevention in case of hazardous chemical release into the atmosphere,especially in densely built-up regions with large populations,a multi-scale urban atmospheric dispersion model was established.Three numerical dispersion experiments,at horizontal resolutions of 10 m,50 m and 3000 m,were performed to estimate the adverse effects of toxic chemical release in densely built-up areas.The multi-scale atmospheric dispersion model is composed of the Weather Forecasting and Research （WRF） model,the Open Source Field Operation and Manipulation software package,and a Lagrangian dispersion model.Quantification of the adverse health effects of these chemical release events are given by referring to the U.S.Environmental Protection Agency＇s Acute Exposure Guideline Levels.The wind fields of the urban-scale case,with 3 km horizontal resolution,were simulated by the Beijing Rapid Update Cycle system,which were utilized by the WRF model.The sub-domain-scale cases took advantage of the computational fluid dynamics method to explicitly consider the effects of buildings.It was found that the multi-scale atmospheric dispersion model is capable of simulating the flow pattern and concentration distribution on different scales,ranging from several meters to kilometers,and can therefore be used to improve the planning of prevention and response programs.     

高分七号卫星激光测高仪无场几何定标法

无场几何定标是未来多波束激光测高卫星面临的一个关键问题。本文针对高分七号（GF-7）线性体制全波形激光测高仪,提出了一种基于地形和波形匹配的无场分步定标方法。在深入分析高分七号卫星激光测高仪特点的基础上,构建了严密几何定位模型,采用公开版的地形参考数据和某地区1∶2000高精度的DOM和LiDAR-DSM基础地理信息成果,开展了在轨无场几何定标试验,显著提高了高分七号卫星激光测高数据精度。在2020年上半年受新冠肺炎影响未进行外场定标期间,有效解决了激光测高数据处理无定标参数的实际困难。本文对无场定标结果与高分七号实际外场定标结果进行对比验证,结果表明,无场定标结果与实际落点位置的平面误差为11.597±3.693 m,最小值为7.115 m,平坦地区高程精度优于0.3 m,虽然略低于外场定标结果,但能满足1∶10 000高程控制点测量需求。