Effect of dietary docosahexaenoic acid on lipogenesis and lipolysis in black sea bream, Acanthopagrus schlegeli

1 IntroductionIt is well known that varying dietary fatty acidprofile affects the tissue fatty acid composition and e-ven the growth performance in fish ( Bell et al.,2002; Figueiredo -Silva et al., 2005; Harel andPlace, 2003; Schulz et al., 2005; Tocher et al.,2003). Docosahexaenoic acid (DHA), an importantessential fatty acid for marine species, has the effectnot only on the fatty acid profile of fish body tissue,but also on biological and physiological conditions(Ishizaki et al., 2000; …     

Distribution of cytochrome P4501A (CYP1A) in the tissues of Baltic ringed and grey seals

Information about the expression of CYP1A in wildlife species is essential for understanding the impact of organochlorine exposure on the health status of an exposed population. Therefore, we aimed at characterising a putative CYP1A enzyme expression in both hepatic and extrahepatic tissues of ringed and grey seals from the Baltic Sea and from less polluted waters. The cellular localisation of CYP1A was identified using a monoclonal antibody against scup P4501A1 (MAb 1-12-3). Immunohistochemical staining showed the highest level of CYP1A expression in liver hepatocytes, and the second highest level in the endothelial cells of capillaries and larger blood vessels in the liver and other organs. The most frequent and strongest staining was found in Baltic ringed seals. Although CYP1A-positive staining was observed in only a few tissues in the other seal populations, it was more intense in Baltic grey seals than in Canadian grey seals. The CYP1A enzyme activity, expressed as ethoxyresorufin O-deethylation (EROD), followed a similar tissue distribution and geographical pattern as the immunohistochemistry with clearly elevated EROD activities in most tissues of both Baltic seal populations. Immunochemical characterisation by immunoblotting confirmed the presence and elevation pattern of a putative CYP1A protein in ringed and grey seals, supporting our findings using other methods. The evenly distributed elevation of CYP1A expression among most of the tissues examined indicates that Baltic seals are exposed to CYP1A inducing agents affecting the whole body. This may result in an increased or decreased toxic potential of foreign substances, which may ultimately determine the biological effects of the contaminants.     

敌百虫对两种赤潮微藻生长的毒性效应研究

敌百虫是使用最为广泛的有机磷农药之一,在水产养殖中主要用于防治甲壳类寄生虫。本文研究了72 h的急性暴露以及25 d的亚慢性暴露下,敌百虫对两种赤潮微藻海洋卡盾藻和锥状斯氏藻生长的影响,以了解敌百虫对海洋微藻的毒性及海洋初级生产力的影响。在急性暴露下,高质量浓度敌百虫能明显抑制两种藻细胞的生长,而低质量浓度则对藻细胞的生长影响不明显,敌百虫对海洋卡盾藻和锥状斯氏藻生长的72 h EC50分别为32.1 mg/L和22.1 mg/L。在亚慢性暴露下,两种藻细胞对敌百虫的敏感性相近,高质量浓度组(25 mg/L)的藻细胞在暴露的第7~9天就全部死亡;1 mg/L的低质量浓度组,藻细胞的生长状况与对照组相近;5 mg/L和10 mg/L质量浓度组在暴露初期对藻细胞生长具有抑制作用,但在暴露后期则明显刺激了藻细胞的生长。本研究说明水环境背景质量浓度下的敌百虫不会抑制浮游植物的生长,但施药一段时间后,可刺激藻细胞的生长,从而增加了引发赤潮的风险。     

西藏尼雄地区水系沉积物地球化学特征及异常分布规律

西藏尼雄地区位于拉达克—冈底斯拉萨—腾冲陆块,区内已发现尼雄特大型富磁铁矿、日阿中型铜多金属矿等矿床.文章以1：5万水系沉积物地球化学调查为基础,总结水系沉积物地球化学特征及分布规律.研究发现区内成矿作用与亲铜元素和铁族元素有关,二叠系昂杰组、拉噶组是区内金矿的有利地层,敌布错组是寻找银、锌、铅、镉矿产的最佳地层,所圈化探异常与已有矿（化）点区域套合较好.