An experimental investigation on diffusion of water in haplogranitic melts

  The diffusivity of water has been investigated for a haplogranitic melt of anhydrous composition Qz₂₈Ab₃₈Or₃₄ (in wt %) at temperatures of 800–1200°C and at pressures of 0.5–5.0 kbar using the diffusion couple technique. Water contents of the starting glass pairs varied between 0 and 9 wt %. Concentration-distance profiles for the different water species (molecular water and hydroxyl groups) were determined by near-infrared microspectroscopy. Because the water speciation of the melt is not quenchable (Nowak 1995; Nowak and Behrens 1995; Shen and Keppler 1995), the diffusivities of the individual species can not be evaluated directly from these profiles. Therefore, apparent chemical diffusion coefficients of water (D _water) were determined from the total water profiles using a modified Boltzmann-Matano analysis. The diffusivity of water increases linearly with water content <3 wt % but exponentially at higher water contents. The activation energy decreases from 64 ± 10 kJ/mole for 0.5 wt % water to 46 ± 5 kJ/mole for 4 wt % water but remains constant at higher water contents. A small but systematic decrease of D _water with pressure indicates an average activation volume of about 9 cm³/mole. The diffusivity (in cm²/s) can be calculated for given water content (in wt %), T (in K) and P (in kbar) by

Toxinogenicity and cytotoxicity of <Emphasis Type="Italic">Alternaria,Aspergillus</Emphasis> and <Emphasis Type="Italic">Penicillium</Emphasis> moulds isolated from working environments

There is currently limited research available on the secondary metabolites of moulds in workplaces. The aim of this study was to determine the mould contamination in museums (N = 4), composting plants (N = 4) and tanneries (N = 4) and the secondary metabolite profiles of Alternaria, Aspergillus and Penicillium isolates from these workplaces. Alternaria, Aspergillus and Penicillium species were identified using the ITS1/2 sequence of the rDNA region. Mould metabolites were quantitatively analysed on standard laboratory medium and mineral medium containing materials specific to each workplace using liquid chromatography-mass spectrometry. We also examined the cytotoxicity of the moulds using MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) assays. Air microbiological contamination analyses showed a number of microorganisms, ranging from 2.4 × 10³ CFU m^?3 (composting plants) to 6.8 × 10⁴ CFU m^?3 (tanneries). We identified high percentages of Alternaria, Aspergillus and Penicillium moulds (air 57–59%, surfaces 10–65%) in all workplaces. The following moulds were the most cytotoxic (>90%): Alternaria alternata, A. limoniasperae, Aspergillus flavus, Penicillium biourgeianum, P. commune and P. spinulosum. The same mould species isolated from different working environments exhibited varying toxigenic and cytotoxic properties. Modifying the culture medium to simulate environmental conditions most often resulted in the inhibition of secondary metabolite production. Moulds isolated from the working environments produced the following mycotoxins (ng g^?1): chanoclavines (0.28–204), cyclopiazonic acid (27.1–1045), fumigaclavines (0.33–10,640,000), meleagrin (0.57–13,393), roquefortins (0.01–16,660), rugulovasines (112–220), viridicatin (0.12–957), viridicatol (4.23–2753) and quinocitrinines (0.07–1104), which may have a negative impact on human health.     

Chemical changes in fluid composition due to CO2 injection in the Altmark gas field: preliminary results from batch experiments

Dissolution?Cprecipitation phenomena induced by CO₂ injection to Altmark Permian sandstone were observed through laboratory experiments carried out under simulated reservoir conditions (125?°C and 50 bars of pressure). The rock sample was collected from the Altmark gas reservoir, which is being considered for enhanced gas recovery. Two sets of experiments were performed with pulverized rock samples in a closed batch reactor with either pure water (run 1) or 3?M aqueous NaCl solution (run 2) and reacted with injected CO₂ for 3, 5, and 9?days. The liquid samples were analyzed by inductively coupled plasma optical emission spectroscopy and total reflection X-ray fluorescence, where the latter proved to be a feasible alternative to conventional analytical techniques, especially since only small sample volumes (about 10???l) are needed. Chemical analysis for both fluids (water and NaCl brine) indicated a significant dissolution of calcite and anhydrite in the solution, which might be a crucial process during CO₂ injection. The brine solution enhanced the dissolution of calcite and anhydrite compared to pure water at the beginning of the reaction. Moreover, the progressive higher Si⁴⁺/Al³⁺ molar ratios (in average by a factor of 3) in the brine experiments indicated quartz dissolution. Thermodynamic calculations of mineral saturation indices highlighted the dissolution of the Ca-bearing minerals, which was in agreement with experimental results. Modeling enabled an evaluation of the dissolution processes of minerals in a low-salinity region, yet hindrances to model more saline conditions emphasize the need for further laboratory studies in order to parameterize models for deep aquifer conditions.     

Sonication as an aid in cleaning cladoceran remains extracted from sediment cores

Ultrasonic treatment (sonication) of the Szczecin Lagoon sediment samples processed for examination of cladoceran remains is described. As opposed to cladoceran remains from non-sonicated sediment, those extracted from the ultrasound-treated samples were clean, easily stained, clearly visible in microscope slides and identifiable. Sonication is therefore recommended as an aid in rendering cladoceran remains free of adhered particles that obscure the specimens and complicate their identification in some sediments.     

Changes in The Dynamics And Thermal Regime of The Permafrost and Active Layer of The High Arctic Coastal Area in North‐West Spitsbergen,Svalbard

In this paper changes in the active layer of the high arctic coastal area in north‐west Spitsbergen, Svalbard are described. Analysis includes both the ground thawing depth and its near‐surface thermal structure. The study was conducted on the Kaffiøyra Plain (Svalbard) at several fixed sites, which represent places typical of the region: a sandy beach, a tundra plain and a moraine ridge. The results show that in recent years, at two measurement points a significantly deeper thawing was observed. In 1996–2012 on the beach and on the moraine the trend of active layer thickness change was +1.3 cm yr^?1 and +2.5 cm yr^?1, respectively. Generally, in the years 2008–2012 the mean thickness of the active layer in the Kaffiøyra region increased by 3% to 6%. Even at the spatially close sites, within similar environments, there are significant differences in the thickness of the active layer. Measurements show that significant changes also occurred in the thawed ground temperature. Mean values of the observed near‐surface temperature in recent years (2007–2011) were higher by more than 1.0°C, in comparison to the mean in the late 1970s.     

Low‐Ir IAB irons from Morasko and other locations in central Europe: One fall,possibly distinct from IAB‐MG

Differences in texture and discovery location prompted us to analyze 16 irons from Morasko; one from Seeläsgen, known to have a similar composition; and a new mass found at Jankowo Dolne. These were analyzed in duplicate by instrumental neutron‐activation analysis (INAA). The results show that all 18 samples have very similar compositions, distinct from all other IAB irons except Burgavli; we conclude that they are all from a single shower. Eight of the samples were from regions with large amounts of cohenite (but were largely free of inclusions) and six were from samples with very little cohenite; we could find no resolvable difference in composition between these sets, a fact that suggests that the C contents of the metal phases were similar in the two areas. Although Morasko has been classified into the IAB main group (IAB‐MG), its Ir plots well outside the main group field on an Ir‐Au diagram. We considered the possibility that the low Ir reflected contamination by a melt from a IAB region that ponded and experienced fractional crystallization; however, because Morasko has Pt, W, and Ga values that are the same as the highest values in IAB‐MG, we rejected this model. We therefore conclude that Morasko formed from a different melt than the IAB‐MG irons; the Morasko melt was produced by impact heating, but one or more of the main Ir carriers did not melt, leaving much of the Ir in the unmelted residue. Copper is the only element that shows resolvable differences among Morasko samples. Most (13 of 18) samples have 149 ± 4 μg g^?1 Cu, but three have 213 ± 10 μg g^?1; we interpret this to mean that the low‐Cu samples have equilibrated with a Cu‐rich phase, whereas there was none of the latter phase within a few diffusion lengths of the samples with high Cu contents.     

Drainage in heterogeneous sand columns with different geometric structures

This paper discusses multi-step drainage experiments in two heterogeneously packed sand columns (10 × 10 × 20 cm³). Different packing structures were generated using two different sand types. One purpose of the study was to test the influence of packing structures on the movement of water. The second purpose was to assess the quality of predictions for the outflow curves in both columns made with an upscaled model. The heterogeneous structures of the columns can be considered as two opposing extremes. The first column was packed with a random arrangement of two sand types that is not stochastically homogeneous and where a cluster running through the column exists for both materials. The second column was packed with a periodic pattern of coarse-sand inclusions in a fine-sand background and has a clearly defined unit cell. The depth-averaged (2D) spatial distribution of the water content in the columns was monitored during the whole multi-step outflow experiment using neutron radiography. The 3D water content was measured at the steady states by neutron tomography. The experimental results are compared with the model predictions of an upscaled model derived with the homogenization theory. The parameters for the upscaled model are calculated from the hydraulic parameters of the two sand types. These hydraulic parameters were first identified in independent measurements on samples of the two individual sand types, separately. Additionally, the hydraulic parameters of both sands were identified by fitting a numerical model to the measured outflow curves. The different column structures showed a significant effect on water retention and the effective retention function, as water was trapped in the coarse-sand inclusions of the periodic structure. We included this trapping effect in the effective retention function of the upscaled model with an apparent air entry pressure. Contrary to the retention, the different packing structures had no large effect on the dynamic behavior of the outflow. The effective conductivity of the columns is therefore not significantly influenced by the structure. The upscaled models predicted the movement of the averaged water content in the two columns well. This confirms the applicability of upscaled models even if the underlying requirements are not strictly met.