In‐Situ Sonication for Enhanced Recovery of Aquifer Microbial Communities

Sampling methods for characterization of microbial communities in aquifers should target both suspended and attached microorganisms (biofilms). We investigated the effectiveness and reproducibility of low‐frequency (200 Hz) sonication pulses on improving extraction efficiency and quality of microorganisms from a petroleum‐contaminated aquifer in Studen (Switzerland). Sonication pulses at different power levels (0.65, 0.9, and 1.1 kW) were applied to three different groundwater monitoring wells. Groundwater samples extracted after each pulse were compared with background groundwater samples for cell and adenosine tri‐phosphate concentration. Turbidity values were obtained to assess the release of sediment fines and associated microorganisms. The bacterial community in extracted groundwater samples was analyzed by terminal‐restriction‐fragment‐length polymorphism and compared with communities obtained from background groundwater samples and from sediment cores. Sonication enhanced the extraction efficiency up to 13‐fold, with most of the biomass being associated with the sediment fines extracted with groundwater. Consecutive pulses at constant power were decreasingly effective, while pulses with higher power yielded the best results both in terms of extraction efficiency and quality. Our results indicate that low‐frequency sonication may be a viable and cost‐effective tool to improve the extraction of microorganisms from aquifers, taking advantage of existing groundwater monitoring wells.     

SporadicE ionization associated with meteor events

Two meteor events which were sighted in the Gujarat skies of India, were accompained by the visibility of sporadicE ionization on the ionograms recorded at Ahmedabad (Geog. Lat. 23·2°N, long. 72·30°E). The first event was the Dhajala fireball which flashed into the geoatmosphere along an E-N to W-S trail at about 20·40 h IST on 28 January 1976; the closest distance of the ground projection of meteor trail from Ahmedabad was 50km. The other event was a possible meteor group sighted over Ahmedabad on 28 May 1978, at about 21·10 h IST. This work describes the nature of the sporadicE ionization observed on Ahmedabad ionograms during the two events. Features of theEs echo during the Dhajala event which indicate that it could be of meteoric origin are discussed. Meteor theory is used to relate the observed ionization with the physical dimensions of the Dhajala meteorite as obtained by other workers.     

Buchbesprechungen

Ohne Zusammenfassung     

An unexploited population of oysters,Ostrea lutaria Hutton,from foveaux strait

Settlement of Ostrea lutaria on sand‐cement‐plastered asbestos plates held in steel frames on the bottom of Foveaux Strait was studied from March 1965 to April 1967. Spat mortality over the period of plate exposure (about one month) was very low (maximum of 3 percent) and was greatest shortly after settlement. There was no evidence that mortality was due to predation. Essentially the same seasonal pattern of settlement was observed each year. Eighty to 90 percent of spat settled between mid December and mid February in both summers; settlement occurred as early as October; some was observed as late as July.

Results of field experiments suggest some very tentative conclusions on the settlement behaviour of O. lutaria: (1) The larvae are benthopelagic and remain on the bottom in strong current conditions. (2) Light stimulates swimming of larvae in still water. (3) In still water and low light intensities of the area larvae tend to swim upward toward the light. (4) In still water and dim light of the area larvae settle predominantly on under surfaces. (5) In still water in the absence of light larvae settle predominantly on upper surfaces. (6) Under the conditions of current and light in the multiplate frames used to sample spatfall here, larvae settle mainly on upper surfaces. (7) Larvae tend to crawl up inclined surfaces before settling.

The results indicate that larval behaviour may be changed by small alterations of light intensity and current. The contradictory nature of surface angle preferences found by other workers for various species of Ostrea and Crassostrea may have been due to such small variations caused by the experimental techniques. It is possible that the settlement behaviour of all species of these genera is similar.     

A method to reduce the influence of ice-rafted debris on a grain size record from northern Fram Strait, Arctic Ocean

The granulometric composition of terrigenous deep-sea sediments provides information on current speed if certain frame conditions are fulfilled. These include that current transport is the only transport process. At high latitudes this type of investigation is impaired due to the influence of ice-rafted debris (IRD) which contaminates the current-sorted grain size fractions. This study presents a new method that addresses this problem by setting the ice-rafted sand in relation to the silt of both current- and ice-transported origin. Deviations from the resulting regression function are then used to determine the behaviour of the silt mean grain size as a function of current speed largely independent from IRD bias. The study is based on sediments from the Yermak Plateau, Arctic Ocean, a region influenced by IRD brought with the south-headed Transpolar Drift and by north-directed bottom currents. The IRD correction results in displaying changes of current speed at much higher clarity; climate forcing of the currents becomes more evident. For example, the 8200 year cold event shows up as a major event in the corrected record whereas it is hardly visible in the original record.     

Klüfte und Talrichtungen im Turon des südöstlichen Münsterlandes

Zusammenfassung Am Südostrand des Münsterländer Kreidebeckens fließen alle größeren Wasserläufe im Bereich der Turonkalke nach NNE oder NE, obwohl das allgemeine Gelände- und Schichtengefälle nach NNW gerichtet ist. Die Ursachen für diese Abweichung sind im Mechanismus der Felserosion zu suchen, der im wesentlichen durch die Eigentümlichkeiten der Karstgerinne und durch die Gesteinszerklüftung bestimmt wird. Bei den Detailuntersuchungen geben sich komplexe Beziehungen zwischen Fließrichtung und Kluftrichtungen zu erkennen.Besonders bedeutend für die Entstehung der NNE gerichteten Täler sind die 170°- und 30°-streichenden Klüfte.

---

On the south-east border of the Cretaceous basin of Munster, Westfalia, the current directions of streams in the area of Turon-limestone go to NNE or NE, although the main dipping of land surface as well as bedding planes points towards NNW. This divergence is caused by the mechanism of rock erosion, which is determined by peculiarities of subterranean stream channels and joints in limestone. The investigations show complex relations between flow direction and direction of joints.The 170°- and 30°-striking joints are significant of the origin of the NNE direction of the valleys.

---

Résumé Dans le Turonien au bord sud-est du bassin Crétacé de Münster (Westphalie) toutes les rivières coulent en direction nord-nord-est ou nord-est, quoique la surface morphologique générale et les couches soient penchées en direction nord-nord-ouest. Cette divergence est expliquée par le mécanisme de l'érosion qui dépend principalement des fentes et du caractère des ruisseaux karstiques. Pendant ces études on a pu observer des relations complexes entre la direction des courants et la direction des fentes.Les fentes en direction nord-nord-est (30°) et sud-sud-est (170°) ont considérablement influencé l'origine des vallées.

---

- - , -. . . , NNE, , 170° 30°.

---

---

Herrn Professor Dr.Roland Brinkmann zum 70. Geburtstag gewidmet.     

The bonding of ferrous iron to sulfur and oxygen in tetrahedral coordination: A comparative study using SCF Xα scattered wave molecular orbital calculations

Molecular quantum mechanical calculations have been performed on high-spin ferrous iron tetrahedrally coordinated to sulfur and oxygen, respectively. The molecular orbital energies obtained from the calculations are compared with experimental optical and X-ray emission spectra. Good agreement was found between calculated and experimental spectral transition energies for the optical absorption spectra of Fe²⁺ in sphalerite, of Fe²⁺ in FeAl₂O₄, staurolite and (Zn, Fe)O, and for the FeKβ X-ray emission spectra of FeCr₂O₄. This both clarified interpretation of the spectra and established the validity of the calculations. Distinct differences occur in the molecular orbital structures of the sulfide and oxide clusters. In the sulfide, the crystal field type (mainly Fe 3d) molecular orbitals lie within the nonbonding (mainly S 3p) orbitais in energy, whereas in the oxide, they lie well above the 02p nonbonding orbitals. This also results in a wider valence band in the oxide than in the sulfide. The crystal field type (Fe 3d) molecular orbitais have more ligand character in the sulfide than the oxide and the chalcophilic properties of iron are partly attributed to this observation.