Glutathione-mediated chlorothalonil detoxification in channel catfish gills

Maintenance of the glutathione (GSH) pathway is critical in guarding against the toxic effects of a variety of pollutants. However, despite their importance as an initial line of defense against waterborne toxicants, little is known regarding the role of fish gills in GSH-mediated xenobiotic metabolism. In these studies, the role of channel catfish gills in the detoxification of 2,4,5,6-tetrachloroisophthalonitrile (chlorothalonil), an electrophilic fungicide, was examined. Chlorothalonil was metabolized in vitro by gill cytosolic and microsomal glutathione S-transferases (GSTs). Chlorothalonil metabolism by cytosolic fructions was reduced markedly when GSH was omitted from reaction mixtures. Microsomal metabolism of chlorothalonil did not occur in the absence of GSH and in the presence of an NADPH-regenerating system lacking GSH. Channel catfish exposed in vivo to chlorothalonil had increased gill GSH and cysteine concentrations after 72 h exposure, and increased gill gamma-glutamylcysteine synthetase (GCS) activity at 144 h of exposure. Our results show that the gills play an important role in the metabolism and detoxification of chlorothalonil in the channel catfish. Catfish can maintain elevated gill GSH concentrations in the presence of chlorothalonil by increasing gill cysteine concentrations.     

GSTA is a major glutathione S-transferase gene responsible for 4-hydroxynonenal conjugation in largemouth bass liver

We have previously shown that largemouth bass (Micropterus salmoides) has a remarkable ability to conjugate 4-hydroxy-2-nonenal (4HNE), a mutagenic and cytotoxic alpha,beta-unsaturated aldehyde produced during the peroxidation of lipids. In addition, we have isolated a glutathione S-transferase cDNA (bass GSTA) that encodes a recombinant protein which is highly active in 4HNE conjugation and structurally similar to plaice (Pleuronectes platessa) GSTA. In the present study, HPLC-GST subunit analysis revealed the presence of at least two major GST isoforms in bass liver, with one peak constituting 80% of the total bass liver GST protein. Liquid chromatography mass spectrometry (LC-MS) and electrospray ionization analysis of the major bass GST subunit yielded a molecular weight of 26,396 kDa. Endo-proteinase Lys-C digestion and Edman degradation protein sequencing of this GST peak demonstrated that this protein was encoded by bass GSTA. Analysis of genomic DNA fragments isolated by nested PCR indicated the presence of a GST gene cluster in bass liver that contained GSTA, and was similar to a GST gene cluster characterized by Leaver et al., in plaice. Collectively, our data indicates the presence of a major GST in bass liver involved in the protection against oxidative stress. This GST is part of a gene cluster that may be conserved in certain freshwater and marine fish.     

Biological disturbance and camouflage of sedimentary features on the Northeast United States slope and rise

Evidence from over 200 sediment cores, numerous submersible dives, and bottom photographs prove that bioturbation and bioerosion are ongoing processes affecting northeastern U.S. continental slope and rise sedimentation. Evidence of biological activity was found in greater than 95% of the cores examined. Submersible dive observations reveal that the results of biological activity often dominate sea-floor microtopography. Bioturbation can disturb sediments several centimeters deep in a matter of seconds and is in some areas the primary sediment transport mechanism. Many cores with sandy intervals were profoundly disturbed by bioturbation. Biologically camouflaged sand-rich intervals can easily be missed by visual observation.     

Multiple P–T–d–t paths reveal the evolution of the final Nuna assembly in northeast Australia

The final assembly of the Mesoproterozoic supercontinent Nuna was marked by the collision of Laurentia and Australia at 1.60 Ga, which is recorded in the Georgetown Inlier of NE Australia. Here, we decipher the metamorphic evolution of this final Nuna collisional event using petrostructural analysis, major and trace element compositions of key minerals, thermodynamic modelling, and multi-method geochronology. The Georgetown Inlier is characterised by deformed and metamorphosed 1.70–1.62 Ga sedimentary and mafic rocks, which were intruded by c. 1.56 Ga old S-type granites. Garnet Lu–Hf and monazite U–Pb isotopic analyses distinguish two major metamorphic events (M1 at c. 1.60 Ga and M2 at c. 1.55 Ga), which allows at least two composite fabrics to be identified at the regional scale—c. 1.60 Ga S1 (consisting in fabrics S1a and S1b) and c. 1.55 Ga S2 (including fabrics S2a and S2b). Also, three tectono-metamorphic domains are distinguished: (a) the western domain, with S1 defined by low-P (LP) greenschist facies assemblages; (b) the central domain, where S1 fabric is preserved as medium-P (MP) amphibolite facies relicts, and locally as inclusion trails in garnet wrapped by the regionally dominant low-P amphibolite facies S2 fabric; and (c) the eastern domain dominated by upper amphibolite to granulite facies S2 foliation. In the central domain, 1.60 Ga MP–medium-T (MT) metamorphism (M1) developed within the staurolite–garnet stability field, with conditions ranging from 530–550°C at 6–7 kbar (garnet cores) to 620–650°C at 8–9 kbar (garnet rims), and it is associated with S1 fabric. The onset of 1.55 Ga LP–high-T (HT) metamorphism (M2) is marked by replacement of staurolite by andalusite (M2a/D2a), which was subsequently pseudomorphed by sillimanite (M2b/D2b) where granite and migmatite are abundant. P–T conditions ranged from 600 to 680°C and 4–6 kbar for the M2b sillimanite stage. 1.60 Ga garnet relicts within the S2 foliation highlight the progressive obliteration of the S1 fabric by regional S2 in the central zone during peak M2 metamorphism. In the eastern migmatitic complex, partial melting of paragneiss and amphibolite occurred syn- to post- S2, at 730–770°C and 6–8 kbar, and at 750–790°C and 6 kbar, respectively. The pressure–temperature–deformation–time paths reconstructed for the Georgetown Inlier suggest a c. 1.60 Ga M1/D1 event recorded under greenschist facies conditions in the western domain and under medium-P and medium-T conditions in the central domain. This event was followed by the regional 1.56–1.54 Ga low-P and high-T phase (M2/D2), extensively recorded in the central and eastern domains. Decompression between these two metamorphic events is ascribed to an episode of exhumation. The two-stage evolution supports the previous hypothesis that the Georgetown Inlier preserves continental collisional and subsequent thermal perturbation associated with granite emplacement.     

Science-based and stakeholder-driven marine protected area network planning: A successful case study from north central California

The planning process for California's Marine Life Protection Act in north central California represents a case study in the design of a regional component of a statewide network of marine protected areas (MPAs) for improved ecosystem protection. We describe enabling factors, such as a legislative mandate, political will, and adequate capacity and funding that fostered a successful planning process. We identify strategic principles that guided the design of a transparent public planning process that delivered regional MPA network proposals, which both met science guidelines and achieved a high level of support among stakeholders. We also describe key decision support elements (spatial data, planning tools, and scientific evaluation) that were essential for designing, evaluating, and refining alternative MPA network proposals and for informing decision-makers.     

Three-dimensional bounding surface architecture and lateral facies heterogeneity of a wet aeolian system: Entrada Sandstone,Utah

Aeolian deposits form noteworthy reservoirs (for example, Norphlet Formation and Rotliegend Group) in hydrocarbon extraction and carbon capture and storage contexts, but stratigraphic architecture imparts significant heterogeneity. Bounding surfaces result from autogenic and allogenic controls and can represent important changes in dune-field dynamics. To further evaluate the impacts of facies heterogeneity and flow-inhibiting bounding surfaces on reservoir performance and reconstruct ancient erg evolution, the stratigraphic architecture of aeolian systems must continue to be studied at multiple scales. This study pairs traditional methods (for example, measured stratigraphic sections) with advanced technologies (for example, drone-derived outcrop models) to precisely resolve the metre to kilometre-scale three-dimensional stratigraphic architecture of wet aeolian Middle Jurassic Entrada Sandstone outcrops located at Rone Bailey Mesa near Moab, Utah, USA. Five facies are identified, primarily based on sedimentary fabrics, and are grouped into three associations named dune, sabkha and sand sheet. Statistical analyses of gamma-ray spectrometer and automated mineralogy data indicate a distinct mineralogical difference between dune (quartz-rich) and sabkha (more feldspathic) packages, suggesting that gamma-ray logs may be used to better predict facies distribution in the subsurface. Seven modelled super bounding surfaces are planar to undulatory, with no perceived spatial trends. Five modelled interdune migration surfaces are undulatory but exhibit an average 0.09° angle of climb roughly parallel to the palaeocurrent direction. Two modelled superposition surfaces are linear to sinuous in plan-view. Laterally discontinuous sabkha packages observed are interpreted to be remnants of closed, damp, interdune flats located between ca 8.5 to 17.0 m tall, sinuous, transverse bedforms or patches of such bedforms. Based on stratigraphic architecture interpretations, the Entrada Sandstone preserves signals of allogenic forcing and localized autogenic bedform cannibalization of the substrate. The findings of this study, some of which are not commonly recognized in wet aeolian facies models, enhance the understanding of erg evolution and can parameterize static models of aeolian reservoirs.     

Power spectrum for the small-scale Universe

The first objects to arise in a cold dark matter (CDM) universe present a daunting challenge for models of structure formation. In the ultra small-scale limit, CDM structures form nearly simultaneously across a wide range of scales. Hierarchical clustering no longer provides a guiding principle for theoretical analyses and the computation time required to carry out credible simulations becomes prohibitively high. To gain insight into this problem, we perform high-resolution  ( N = 720³–1584³)  simulations of an Einstein–de Sitter cosmology where the initial power spectrum is   P ( k ) ∝ k ⁿ ,  with  −2.5 ≤ n ≤− 1  . Self-similar scaling is established for   n =−1  and −2 more convincingly than in previous, lower resolution simulations and for the first time, self-similar scaling is established for an   n =−2.25  simulation. However, finite box-size effects induce departures from self-similar scaling in our   n =−2.5  simulation. We compare our results with the predictions for the power spectrum from (one-loop) perturbation theory and demonstrate that the renormalization group approach suggested by McDonald improves perturbation theory's ability to predict the power spectrum in the quasi-linear regime. In the non-linear regime, our power spectra differ significantly from the widely used fitting formulae of Peacock & Dodds and Smith et al. and a new fitting formula is presented. Implications of our results for the stable clustering hypothesis versus halo model debate are discussed. Our power spectra are inconsistent with predictions of the stable clustering hypothesis in the high- k limit and lend credence to the halo model. Nevertheless, the fitting formula advocated in this paper is purely empirical and not derived from a specific formulation of the halo model.     

The Canary Eddy Corridor: A major pathway for long-lived eddies in the subtropical North Atlantic

We report, from remote sensing and in situ observations, a new type of permanent structure in the eastern subtropical Atlantic Ocean, that we call the “Canary Eddy Corridor”. The phenomenon, is a zonal long-lived (>3 months) mesoscale eddy corridor, whose source is the flow perturbation of the Canary Current and the Trade Winds at the Canary Islands. The latitudinal range of the corridor spans 22°N–29°N and extends from the Canaries to at least 32ºW, near the mid-Atlantic. This is the main region of long-lived westward-propagating eddies in the subtropical northeast Atlantic. From a age-distribution study we observe that at least 10% of mesoscale eddies in this region are long-lived, with a dominance of anticyclones over cyclones. Another four westward-propagating eddy corridors were also detected: two small corridors north and south of the Azores Front; a small zonal corridor located near 31ºN, south of the island of Madeira; and a small corridor located near the Cape Blanc giant filament. The existence of these corridors may change, at least for the northeastern subtropical Atlantic, the general idea that mesoscale eddies are disorganized, ubiquitous structures in the ocean. The Canary Eddy Corridor constitutes a direct zonal pathway that conveys water mass- and biogeochemical properties offshore from the Canary Island/Northwest Africa upwelling system, and may be seen as a recurrent offshore pump of organic matter and carbon to the oligotrophic ocean interior. Estimates of volume and mass transport indicate that Canary Eddy Corridor westward transport is more than one-fourth of the southward transport of the Canary Current. The westward transport of kinetic energy by the eddies of the Canary Corridor is as important as the southward transport by the Canary Current. The total primary production related to the Corridor may be as high as the total primary production of the northwest Africa upwelling system for the same latitude range.     

Komatiites and nickel sulfide ores of the Black Swan area,Yilgarn Craton,Western Australia. 3: Komatiite geochemistry,and implications for ore forming processes

The Black Swan komatiite sequence is a package of dominantly olivine-rich cumulates with lesser volumes of spinifex textured rocks, interpreted as a section through an extensive komatiite lava flow field. The sequence hosts a number of nickel sulfide orebodies, including the Silver Swan massive shoot and the Cygnet and Black Swan disseminated orebodies. A large body of whole rock analyses on komatiitic rocks from the Black Swan area has been filtered for metasomatic effects. With the exception of mobile elements such as Ca and alkalis, most samples retain residual igneous geochemistry, and can be modelled predominantly by fractionation and accumulation of olivine. Whole rock MgO–FeO relationships imply a relatively restricted range of olivine compositions, more primitive than the olivine which would have been in equilibrium with the transporting komatiite lavas, and together with textural data indicate that much of the cumulus olivine in the sequence was transported. Flow top compositions show evidence for chromite saturation, but the cumulates are deficient in accumulated chromite. Chromite compositions are typical of those found in compound flow-facies komatiites, and are distinct from those in komatiitic dunite bodies. Incompatible trace element abundances show three superimposed influences: control by the relative proportion of olivine to liquid; a signature of crustal contamination and an overprint of metasomatic introduction of LREE, Zr and Th. This overprint is most evident in cumulates, and relatively insignificant in the spinifex rocks. Platinum and palladium behaved as incompatible elements and are negatively correlated with MgO. They show no evidence for wholesale depletion due to sulfide extraction, which was evidently restricted to specific lava tubes or pathways. The lack of correspondence between PGE depletion and contamination by siliceous material implies that contamination alone is insufficient to generate S-saturation and ore formation in the absence of sulfide in the assimilant. Contamination signatures in spinifex-textured rocks may be a guide to Ni-sulfide mineralisation, but are not entirely reliable in the absence of other evidence. The widespread vesicularity of the sequence may be attributable to assimilated water rather than to primary mantle-derived volatiles, and cannot be taken as evidence for primary volatile-rich magmas. The characteristic signature of the Black Swan Succession is the presence of highly localised disseminated sulfide within a sequence showing more widespread evidence for crustal contamination and interaction with its immediate substrate. This has important implications for the applicability of trace element geochemistry in exploration for komatiite-hosted nickel deposits.Electronic Supplementary Material Supplementary material is available in the online version of this article at Editorial handling: Peter Lightfoot