Differences in the acute toxicities of tributyltin between the Caprellidea and the Gammaridea (Crustacea: Amphipoda)

Tests for the acute toxicity of tributyltin (TBT) were conducted on amphipod crustaceans collected from Otsuchi Bay, Japan. Five species of caprellids and three species of gammarids, which belong to a closely related ecological niche, were used for the exposure experiments at seven test concentrations (0, 0.001, 0.01, 0.1, 1, 10 and 100 microg TBTCl/l) for 48 h at 20 degrees C. The 48-h LC50 values of the caprellids were 1.2-6.6 microg TBTCl/l, and these were significantly lower than those of the gammarids (17.8-23.1 microg TBTCl/l). This suggests that caprellids are more sensitive to TBT than gammarids. Furthermore, the proportions of TBT and its derivatives, dibutyltin (DBT) and monobutyltin (MBT), were measured in the amphipods collected from Otsuchi Bay. In the caprellids, TBT was the predominant compound, accounting for 72% of the total butyltin which reflected the butyltin ratio in seawater, while in the gammarids, TBT's breakdown products (DBT and MBT) predominated, accounting for 75% of the total butyltin. This difference suggests that caprellids may have lower metabolic capacity to degrade TBT than gammarids. Therefore, the difference in sensitivity to TBT among the amphipods is thought to be related to the species-specific capacity to metabolize TBT.     

Assessing the suitability of data from Sentinel-1A and 2A for crop classification

Sentinel-1A C-SAR and Sentinel-2A MultiSpectral Instrument (MSI) provide data applicable to the remote identification of crop type. In this study, six crop types (beans, beetroot, grass, maize, potato, and winter wheat) were identified using five C-SAR images and one MSI image acquired during the 2016 growing season. To assess the potential for accurate crop classification with existing supervised learning models, the four different approaches namely kernel-based extreme learning machine (KELM), multilayer feedforward neural networks, random forests, and support vector machine were compared. Algorithm hyperparameters were tuned using Bayesian optimization. Overall, KELM yielded the highest performance, achieving an overall classification accuracy of 96.8%. Evaluation of the sensitivity of classification models and relative importance of data types using data-based sensitivity analysis showed that the set of VV polarization data acquired on 24 July (Sentinel-1A) and band 4 data (Sentinel-2A) had the greatest potential for use in crop classification.     

cDNA cloning of an aryl hydrocarbon receptor from Baikal seals (Phoca sibirica)

Species differences in sensitivity to related planar halogenated aromatic hydrocarbons (PHAH) add significant uncertainty in assessing the ecological risk to aquatic mammals. To investigate mechanisms of PHAH sensitivity in aquatic mammals, we cloned and sequenced the cDNA of Baikal seal aryl hydrocarbon receptor (AHR), an intracellular protein that initiates PHAH-mediated effects. The Baikal seal AHR cDNA has an open reading frame of 843 amino acid residues with a predicted molecular mass of 94.6 kDa. Comparison of AHR amino acid sequences indicated a high degree of sequence conservation (98%) between Baikal and harbor seals. The high conservation of AHRs between Baikal and harbor seals indicates that these seals express AHR proteins closely related structurally. In our previous report (Kim & Hahn, 2002), the dioxin-binding affinity of the harbor seal AHR was at least as high as that of the AHR from a dioxin-sensitive strain of mice, suggesting that this seal species may be sensitive to PHAH effects. This implies that Baikal seal may also be sensitive to dioxin effects.     

Three-year investigations into sperm whale-fall ecosystems in Japan

We report the first study of sperm whale‐fall ecosystems, based on mass sinking of whale carcasses at shelf depths in the northwest Pacific. We conducted three observations over a 2‐year period on replicate sperm‐whale carcasses implanted at depths of 219–254 m off the southern part of Japan from July 2003 to August 2005. The study was made possible by a mass stranding of sperm whales in January 2002, and the subsequent sinking of 12 carcasses in the waters off Cape Nomamisaki. Dense aggregations of unique chemosynthesis‐based fauna had formed around the whale carcasses after 18 months (July 2003). The mytilid mussel Adipicola pacifica was the most abundant macrofaunal species and covered most of the exposed bone surfaces. The general composition of the fauna was similar to that of deep‐water reducing habitats, but none of the species appearing in this study has been found at hydrothermal vents, cold seeps or deep‐water whale falls. A new species of lancelet, which was the first record of the subphylum Cephalochordata from reducing environments, a new species of Osedax; a rarely encountered benthic ctenophore, and a rare gastropod species were discovered at this sperm whale‐fall site. Benthic communities were similar across all the carcasses studied, although the body sizes of the whales were very different. The succession of epifaunal communities was relatively rapid and the sulphophilic stage was considerably shorter than that of other known whale falls.     

Primary productivity in the offshore Oyashio in the spring and summer 1990

Primary productivity was measured byin situ method using¹³C in the offshore Oyashio region in the spring (May) and summer (September) of 1990. Most of the values were within the range of 0.1 to 4 gC 1^–1 h^–1 although a very large value, 7.96 gC l^–1 h^–1, was observed in summer. Most daily primary production fell within the range of 372 to 633 mgC m^–2 d^–1 although a very large value, 2,109 mgC m^–2 d^–1, was observed around the frontal area in summer. Chlorophylla (Chl.a) exceeded 1 g l^–1 in many cases, and the maximum was 4.61 g l^–1 in spring and 7.53 g l^–1 in summer. Most primary productivity per unit Chl.a (photosynthetic assimilation ratio) was within the range of 0.1 to 3 gC gChl.a ^–1 h^–1 although higher values, 3–6 gC gChl.a ^–1 h^–1, were observed where small-size phytoplanktons (<2 m) were dominant. These results were compared with results obtained until now in the Oyashio region. The values beyond the range obtained so far in the offshore region were also observed in this study. Furthermore, it was pointed out that the size composition of phytoplankton community has significant influence on the results of Chl.a and photosynthetic assimilation ratio in the Oyashio region.     

Nitrogen circulation in the water column in the Oyashio region with reference to its high productivity

In order to determine quantitatively the reason for the high productivity in the Oyashio Region, which is the southwest part of the Pacific Subarctic Region, the annual-mean vertical circulation of nitrogen in the region was estimated from the vertical profiles of nitrate, dissolved oxygen and salinity, and sediment-trap data by adapting them to the balance equations. Estimates of the upwelling velocity (1.7×10⁻⁵cm sec⁻¹) and the vertical diffusivity (2.1 cm² sec⁻¹) in the abyssal zone and the primary and secondary productivities (44 and 4 mgN m⁻²day⁻¹, respectively) in the euphotic zone were close to those of previous works. The estimated vertical circulation of nitrogen strongly suggested that, since the divergence (5 mgN m⁻²day⁻¹) is caused by the abyssal convergence (6 mgN m⁻²day⁻¹) and the positive precipitation, the local new production (22 mgN m⁻²day⁻¹) necessarily exceeds not only the sinking flux (10 mgN m⁻²day⁻¹) itself but also the sum of the sinking flux and the downward diffusion of dissolved and particulate organic matter (7 mgN m⁻²day⁻¹) produced probably in the euphotic zone. The important roles of the abyssal circulation, the winter convection, and the metabolic activity in the bathyal zone to support the high productivity in the euphotic zone were clarified quantitatively.     

Butyltin concentrations along the Japanese coast from 1997 to 1999 monitored by Caprella spp. (Crustacea: Amphipoda)

The concentrations of butyltins along the Japanese coastline were investigated from 1997 to 1999, 7 to 9 years after implementation of legislation limiting the use of tributyltin (TBT) in Japan. Seawater was sampled at 0.5 m depth, and Caprella spp. were collected from Sargassum spp. and aquaculture facilities from 18 areas within four broad areas along the coastline of Japan, i.e., the Pacific coast of northern Japan, the coast along the Sea of Japan, Tokyo Bay and adjacent areas, and western Japan. Butyltins (MBT, DBT and TBT) were detected in 32 of the 63 seawater samples with average concentrations of 4.6 ng MBT/l, 4.5 ng DBT/l and 6.8 ng TBT/l, respectively. Butyltin concentrations in seawater from western Japan indicate "hot spots" even in unpopulated areas. Butyltins (MBT, DBT and TBT) were detected in all samples of Caprella spp., varying from 2.3 ng BTs /g wet wt in C. penantis R-type from Tobishima Island in the Sea of Japan to 464 ng BTs /g wet wt in C. decipiens Mayer from Amakusa, western Kyushu. The BT concentrations in Caprella spp. form western Japan were significantly higher than those from other areas, including Tokyo Bay and adjacent areas, where large scale industry and international ports are located. These results indicate that butyltin contamination still remains even in unpopulated areas after the regulation on TBT usage, and that the regulation governing TBT usage since 1990 has not been effective enough to concede recovery of shallow water ecosystems around Japan.     

Validation of ADEOS-II GLI ocean color products using in-situ observations

The Global Imager (GLI) aboard the Advanced Earth Observing Satellite-II (ADEOS-II) made global observations from 2 April 2003 to 24 October 2003. In cooperation with several institutes and scientists, we obtained quality controlled match-ups between GLI products and in-situ data, 116 for chlorophyll-a concentration (CHLA), 249 for normalized water-leaving radiance (nLw) at 443 nm, and 201 for aerosol optical thickness at 865 nm (Tau_865) and Angstrom exponent between 520 and 865 nm (Angstrom). We evaluated the GLI ocean color products and investigated the causes of errors using the match-ups. The median absolute percentage differences (MedPD) between GLI and in-situ data were 14.1–35.7% for nLws at 380–565 nm, 52.5–74.8% nLws at 625–680 nm, 47.6% for Tau_865, 46.2% for Angstrom, and 46.6% for CHLA, values that are comparable to the ocean-color products of other sensors. We found that some errors in GLI products are correlated with observational conditions; nLw values were underestimated when nLw at 680 nm was high, CHLA was underestimated in absorptive aerosol conditions, and Tau_865 was overestimated in sunglint regions. The error correlations indicate that we need to improve the retrievals of the optical properties of absorptive aerosols and seawater and sea surface reflection for further applications, including coastal monitoring and the combined use of products from multiple sensors.     

Formation Process of North Pacific Intermediate Water Revealed by Profiling Floats Set to Drift on 26.7 σθ Isopycnal Surface

Six newly developed floats, which were set to drift on the 26.7 σ_θ isopycnal surface and to profile temperature, salinity and pressure above 1000 dbar once a week, were deployed in the Oyashio and Kuroshio Extension (KE) in order to examine the circulation, formation site and time scale of newly formed North Pacific Intermediate Water (NPIW). The floats were deployed in February or May 2001, and the data from their deployments to December 2002 are analyzed here. Four of the six floats were deployed near the KE axis at around the first meander crest, and they moved eastward to 157°E–176°W at latitudes of 30°N–45°N. The other two floats deployed in the Oyashio water with low-potential vorticity near the south coast of Hokkaido moved southward to reach the KE front and then moved eastward to the same region as the first four floats. The temperature and salinity at 26.7 σ_θ measured by the profiling floats indicate that the source waters of NPIW, Oyashio and Kuroshio waters are drastically mixed and modified in the mixed water region west of 160°E. The floats were separated into the three paths east of 160°E between the Kuroshio Extension front and the north of Water-Mass front (nearly subarctic front). New NPIW is judged to be formed along these three paths since the vertical profiles of temperature and salinity are quite smooth, having a salinity minimum at about 26.7σ_θ along each path. Kuroshio-Oyashio isopycnal mixing ratios of the new NPIW are 7:3, 6:4 and 5:5 at 26.7σ_θ along the southern, middle and northern paths, respectively. Potential vorticity converges to about 14–15 × 10⁻¹¹ m⁻¹s⁻¹ along these paths. The time scale of new NPIW formation is estimated to be 1–1.5 years from the merger of Oyashio and Kuroshio waters to the formation of the new NPIW. This revised version was published online in July 2006 with corrections to the Cover Date.