Metal donation and apo-metalloenzyme activation by stable isotopically labeled metallothionein

Coupled HPLC-ICP-MS has been used to quantitatively study the effects of GSSG and GSH on the ability of metallothionein (MTII) to donate essential and non-essential metals to apo-carbonic anhydrase. Stable isotopically labeled (67)Zn(3)Cd(4) MTII was used to enable Zn donated from MTII to be differentiated from extraneous sources of Zn. Transfer of both (67)Zn and Cd from MTII to apo-carbonic anhydrase was noted in the absence of either GSSG or GSH. GSSG increased the initial transfer of both Zn and Cd. Thereafter, a gradual increase in the (67)Zn content at the expense of Cd was noted over 24-h indicating continued interaction and exchange between MTII and the enzyme commensurate with the relative preferences shown by the proteins for these two metals. Although GSH also increased transfer of (67)Zn from MT it reduced the simultaneous transfer of Cd to the enzyme thereby conferring protection against Cd induced activation.     

Monsoon-induced upwelling off the Vietnamese coast

During the southwest monsoon from July 8 to 28, 2003, an interdisciplinary cruise took place in the central area of Vietnamese upwelling with “MV Nghien Cuu Bien” in the South China Sea. Physical observations in the upwelling area are analyzed with respect to local/regional wind forcing and far field forcing. Nutrients and phytoplankton measurements are discussed with respect to exchange processes between different water masses. The wind-induced coastal upwelling by local wind forcing is much weaker than in the previous years due to weaker-than-normal winds. This can be attributed to the far field forcing of the 2002/2003 El Niño event which modulates the upwelling intensity. The atmospheric conditions reflect the typical situation after an El Niño event which weakens the wind-induced coastal upwelling, reduces the latent heat flux, and results in higher-than-normal sea-surface temperatures. The general circulation pattern during SW monsoon is driven by the spatial asymmetry in the monsoon forcing. The flow pattern is characterized by an upwelling-induced northward undercurrent and a recently detected southward countercurrent. The resulting stretching deformation of this flow pattern forms an offshore jet between ～12°N and 12.5°N and causes a local enhancement of the upwelling intensity. The upwelling due to stretching deformation is a peculiarity, which makes the Vietnamese upwelling area different to other upwelling areas. A budget of the upwelling components is presented: the strongest contribution in 2003 to the Vietnamese upwelling is the dynamical upwelling due to the clockwise rotation of the northward undercurrent. The internal radius of deformation separates the upwelling area from the offshore area as well as different water masses. Mekong River and the Gulf of Thailand waters which are offshore show nutrient depletion. Therefore, high chlorophyll maxima cannot be explained by nutrient supply from river runoff. The dynamical upwelling brings in nutrient-rich Maximum Salinity Water into the euphotic zone. This causes a subsurface chlorophyll maximum between 20 and 40 m water depth along the northward undercurrent. Deflection from the Redfield ratio in the C:N ratio and negative excess nitrogen identifies the region as nitrogen-limited which may favor cyanobacteria blooms. The consequence is a unique feature in new production: in the upwelling area, new production is based on upwelled nitrate, whereas offshore in the nutrient-depleted Mekong and Gulf of Thailand water, new production is based in addition on nitrogen fixation.     

Estimating fluvial wood discharge using time‐lapse photography with varying sampling intervals

Monitoring large wood (LW: width > 10 cm, length > 1 m) in transport within rivers is a necessary next step in the development and refinement of wood budgets and is essential to a better understanding of basin‐wide controls and patterns of LW flux and loads. Monitoring LW transport with coarse interval (≥ 1 min) time‐lapse photography enables the deployment of monitoring cameras at large spatial and long temporal scales. Although less precise than continuous sampling with video, it allows investigators to answer broad questions about basin connectivity, compare drainages and years,and identify transport relationships and thresholds. This paper describes methods to: (i) construct fluvial wood flux curves; (ii) analyze the effects of sample interval lengths on transport estimates; and (iii) estimate total wood loads within a specified time period using coarse‐interval time‐lapse photography. Applying these methods to the Slave River, a large‐volume (10³ m³ s^‐1), low‐gradient (10^{? 2} m km^{? 1}) river in the subarctic (60^° N), yielded the following results. A threshold relationship for wood mobility was located around 4500 m³ s^‐1. More wood is transported on the rising limb of the hydrograph because wood flux declines rapidly on the falling limb. Five‐ and ten‐minute sampling intervals provided unbiased equal variance estimates of 1 min sampling, whereas 15 min intervals were biased towards underestimation by 5–6%, possibly due to periodicity in wood flux. Total LW loads estimated from the 1 min dataset and adjusted for a 15% misdetection rate from 13 July to 13 August are: 1600 ± 200 # pieces, 600 ± 200 m³ and of the order of 1.3 × 10⁵ kg carbon. The total wood load for the entire summer season is probably at least double this estimate because only the second half of the summer was monitored and a large early summer peak freshet was missed. Copyright © 2014 John Wiley & Sons, Ltd.     

Effects of forest stand age on the characteristics of logjams in mountainous forest streams

We measured longitudinal spacing and wood volume of channel‐spanning logjams along 30 1‐km reaches of forest streams in the Colorado Front Range, USA. Study streams flow through old‐growth (> 200 year stand age) or younger subalpine conifer forest. Evaluating correlations between the volume and longitudinal spacing of logjams in relation to channel and forest characteristics, we find that old‐growth forest streams have greater in‐stream wood loads and more jams per kilometer than streams in younger forest. Old‐growth forests have a larger basal area close to the stream and correlate with larger piece diameters of in‐stream wood. Jam volume correlates inversely with the downstream spacing for ramp and bridge pieces that can act as key pieces in jams. Most importantly, old‐growth streams have shorter downstream spacing for ramp and bridge pieces (< 20 m). Our results suggest that management of in‐stream wood and associated stream characteristics can be focused most effectively at the reach scale, with an emphasis on preserving old‐growth riparian stands along lower gradient stream reaches or mimicking the effects of old growth by manipulating the spacing of ramp and bridge pieces. Our finding that average downstream spacing between jams declines as wood load increases suggests that the most effective way to create and retain jams is to ensure abundant sources of wood recruitment, with a particular emphasis on larger pieces that are less mobile because they have at least one anchor point outside the active channel. Copyright © 2014 John Wiley & Sons, Ltd.     

Intercomparison of soil pore water extraction methods for stable isotope analysis

Measurements of δ²H and δ¹⁸O composition of pore waters in saturated and unsaturated soil samples are routinely performed in hydrological studies. A variety of in‐situ and lab‐based pore water extraction methods for the analysis of the stable isotopes of water now exist. While some have been used for decades (e.g. cryogenic vacuum extraction) others are relatively new, such as direct vapour equilibration or the microwave extraction technique. Despite their broad range of application, a formal and comprehensive intercomparison of soil water extraction methods for stable isotope analysis is lacking and long overdue. Here we present an intercomparison among five commonly used lab‐based pore water extraction techniques (high pressure mechanical squeezing, centrifugation, direct vapour equilibration, microwave extraction, and cryogenic extraction). We applied these extraction methods to two physicochemically different soil types that were dried and rewetted with water of known isotopic composition at three different water contents. Our results showed that the extraction approach can have a significant effect on pore water isotopic composition as all methods exhibited significant deviations from the spiked reference water, depending secondarily on the soil type and soil water content. Most pronounced, cryogenic water extraction showed large deviations from the spiked reference water, whereas mechanical squeezing and centrifugation provided results closest to the spiked water for both soil types. We also compared results for each extraction method – where liquid water was obtained – on both an OA‐ICOS and IRMS. Differences between these two analytical instruments were negligible for these organic compound‐free waters. We suggest that users of soil water extraction approaches carefully choose an extraction technique that is suitable for the specific research question, adapted to the dominant soil type and water content of the study. Copyright © 2016 John Wiley & Sons, Ltd.     

Solar Irradiance Models and Measurements: A Comparison in the 220–240?nm wavelength band

Solar irradiance models that assume solar irradiance variations to be due to changes in the solar surface magnetic flux have been successfully used to reconstruct total solar irradiance on rotational as well as cyclical and secular time scales. Modelling spectral solar irradiance is not yet as advanced, and also suffers from a lack of comparison data, in particular on solar cycle time scales. Here, we compare solar irradiance in the 220–240?nm band as modelled with SATIRE-S and measured by different instruments on the UARS and SORCE satellites. We find good agreement between the model and measurements on rotational time scales. The long-term trends, however, show significant differences. Both SORCE instruments, in particular, show a much steeper gradient over the decaying part of cycle 23 than the modelled irradiance or that measured by UARS/SUSIM.     

Field observations of an evolving rip current on a meso-macrotidal well-developed inner bar and rip morphology

The Aquitanian Coast (France) is a high-energy meso-macrotidal environment exhibiting a highly variable double sandbar system. The inner and the outer bar generally exhibit a bar and rip morphology and persistent crescentic patterns, respectively. In June 2007, an intense five-day field experiment was carried out at Biscarrosse Beach. A large array of sensors was deployed on a well-developed southward-oriented bar and rip morphology. Daily topographic surveys were carried out together with video imaging to investigate beach morphodynamic evolution. During the experiment, offshore significant wave height ranged from 0.5 to 3 m, with a persistent shore-normal angle. This paper identifies two types of behavior of an observed rip current: (1) for low-energy waves, the rip current is active only between low and mid tide with maximum mean rip current velocity reaching 0.8 m/s for an offshore significant wave height (H_s) lower than 1 m; (2) for high-energy waves (H_s≈ 2.5–3 m), the rip current was active over the whole tide cycle with the presence of persistent intense offshore-directed flows between mid and high tide. For both low and high-energy waves, very low-frequency pulsations (15–30 min) of the mean currents are observed on both feeder and rip channels.A persistent slow shoreward migration of the sandbar was observed during the experiment while no significant alongshore migration of the system was measured. Onshore migration during the high-energy waves can be explained by different sediment transport processes such as flow velocity skewness, wave asymmetry or bed ventilation. High-frequency local measurements of the bed evolution show the presence of significant (in the order of 10 cm) fluctuations (in the order of 1 h). These fluctuations, observed for both low- and high-energy waves, are thought to be ripples and megaripples, respectively and may play an important but still poorly understood role in the larger scale morphodynamics. The present dataset improves the knowledge of rip dynamics as well as the morphological response of strongly alongshore non-uniform meso-macrotidal beaches.     

Helium isotopes in early Iceland plume picrites: Constraints on the composition of high 3He/4He mantle

A detailed study of the geochemistry of a new suite of early Iceland plume picrites shows that extremely high ³He/⁴He ratios (up to 50 R_a) are found in picrites from Baffin Island and West Greenland. High ³He/⁴He picrites display a wide range in ⁸⁷Sr/⁸⁶Sr (0.70288–0.70403), ¹⁴³Nd/¹⁴⁴Nd (0.51288–0.51308) and incompatible trace element ratios (e.g. La/Sm_n = 0.5–1.6). These overlap the complete range of compositions of mid-ocean ridge basalts and most northern hemisphere ocean island basalts, including Iceland. Crustal contamination modelling in which high-grade Proterozoic crustal basement rocks for the region are mixed with a depleted parent cannot account for the trend displayed by the Baffin Island and West Greenland picrites. This rules out the possibility that the incompatible trace element, Sr and Nd isotope range of the high ³He/⁴He picrites is due to crustal contamination. The compositional range at high ³He/⁴He is also inconsistent with derivation from a primordial-He-rich reservoir that is a residue of ancient mantle depletion. This implies that the composition of the high ³He/⁴He mantle cannot be determined simply by extrapolating ocean island basalt He–Sr–Nd–Pb–Os isotope data.The apparent decoupling of He from trace element and lithophile radiogenic isotope tracers is difficult to attain by simple mixing of a high-[He], high ³He/⁴He reservoir with various depleted and enriched He-poor mantle reservoirs. The possibility that primordial He has diffused into a reservoir with a composition typical of convecting upper mantle cannot be ruled out. If so, the process must have occurred after the development of existing mantle heterogeneity, and requires the existence of a deep, primordial He-rich reservoir.