Optimization of novel hyperthermostable β amylase production by Bacillus subtilis DJ5 using solid agroresidual substrates

Eight different low cost starchy agroresidues namely Barley (B), Wheat bran (WB), Sattu (S), Rice powder (RP), Corn flour (CF), Rice husk (RH), Yellow peas split (YPS) and arrowroot (A) were used for solid culturing of Bacillus subtilis DJ5 for production of novel hyperthermostable β amylase. Various process parameters like initial moisture content, inoculum load, medium pH and incubation temperature affecting enzyme production were optimized to ensure maximum enzyme yield. Only 10 % inoculums load and medium pH of 6.9 was found sufficient to achieve maximum enzyme production in all substrates in a decreasing order, B > WB > S > RP > CF > RH > YPS > A. Optimum β amylase production was highly dependent on initial moisture content of substrate as observed from varying requirement of moisture for different substrates. Only 50 % moisture was sufficient for maximum enzyme production of 84.29 U/gdm in CF. For B, RH, YPS, and A 60 % initial moisture resulted in higher production of 120.34, 35.19, 26.59, and 21.58 U/gdm, respectively, at 37 °C. However, for S and RP higher (70 %) moisture content allowed 113.4 and 85.56 U/gdm enzyme production, respectively. Under optimized conditions, maximum β amylase production was observed after 25 h for A, YPS, RH, RP; 41 h for B, WB, CF, and 45 h for S.     

Temperature dependence of CO2 solubility in high pressure quenched glasses of diopside composition

The temperature dependence of carbon dioxide solubility in glasses of diopside composition, quenched from 20 kbar, has been investigated using a combination of high-temperature mass spectrometry and Raman spectroscopy.CO₂-charged diopside glasses were synthesized in a piston-cylinder apparatus. Because of diffusion of hydrogen through the platinum capsules, significant amounts of H₂O, CH₄ and CO were detected along with CO: in the diopside glasses. All three carbon species show a bimodal release pattern in the mass pyrograms. The CO₂ solubility shows a linear and negative temperature dependence. We do not observe any maxima in the solubility curve as was reported previously (Mysen and virgo, 1980a).None of the additional bands observed in Raman spectra of CO₂-charged diopside glasses compared to those in the spectrum of diopside glass can be assigned to molecular CO₂. These bands are caused by CO^?2₃ ions and indicate that the physical solubility of molecular carbon dioxide is negligible. The bimodal release pattern observed for CO₂ in the mass pyrograms, is consistent with the Raman data which strongly suggests that CO^?2₃ ions are present in at least two distinct sites in the glass.     

Tectonic segmentation of the Burmese-Indonesian arc

Several lines of evidences, which include change in trend and offset in arcs, bathymetry and sedimentation, faulting in the region, change in composition and trend of the line of volcanoes, spatial distribution of earthquakes and change in dip of the Benioff zone, suggest a tectonic segmentation of the Burmese-Indonesian Arc. Two transverse boundary zones, viz., the North Andaman Boundary Zone and the Sunda Boundary Zone separating segments of the arc are identified. Larger than usual thickness of the oceanic crust beneath the Ninetyeast Ridge reduces the average density of the lithosphere carrying the ridge. The buoyancy effect caused by the reduced average density inhibits the subduction of an approximately triangular shaped lithosphere between the Burmese-Indonesian Arc and the Ninetyeast Ridge, which in turn causes tectonic segmentation along the arc. The model predicts and some available focal mechanism solutions confirm left lateral northeast faulting within the Sunda Boundary Zone.     

2.5D Simulation of basin-edge effects on the ground motion characteristics

The effects of basin-edge and soil velocity on the ground motion characteristics have been simulated using 2.5D modeling. One of the most significant advantages of the 2.5D simulation is that 3D radiation pattern can be generated in a 2D numerical grid using double-couple shear dislocation source. Further, 2.5D numerical modeling avoids the extensive computational cost of 3D modeling. The responses of basin-edge model using different soil velocities revealed that surface waves were generated near the edge of the basin and propagated normal to the edge, towards the basin. Further, the results depict increase of amplification, duration and surface wave generation with the decrease in soil velocity.     

Distribution of dissolved carbohydrates and uronic acids in a tropical estuary,India

Carbohydrates including uronic acids are among the active components of dissolved organic carbon, and play an important role in biogeochemical cycling of organic carbon in marine environments. In order to understand their distribution, concentrations of total dissolved carbohydrate (TCHO), dissolved polysaccharide (PCHO), dissolved monosaccharide (MCHO), and dissolved uronic acid (URA) were measured in the Mandovi estuary, west coast of India during the monsoon and premonsoon seasons. The estuary experienced nearly fresh water condition during the monsoon season and marine condition during the pre-monsoon season. Concentrations of TCHO, MCHO and URA ranged from 17.7 to 67.3 μM C, 4.1 to 15.5 μM C and 2.3 to 10.8 μM C, and their contribution to dissolved organic carbon (DOC) varied from ∼11 to 60%, 2.5 to 9.7%, and 1.8 to 5.3%, respectively. PCHO accounted for ∼52 to 92% of the TCHO. Generally, concentrations and yields of TCHO species were greater during the monsoon season. Phytoplankton abundance and bacterial cell numbers influenced the distribution of TCHO in the pre-monsoon season but not during the monsoon season. Transport of TCHO rich (11 to 60%) dissolved organic matter from the Mandovi estuary to the coastal waters during the monsoon season may affect ecosystem function by fueling biological activity of heterotrophic micro-organisms.     

Photocatalytic degradation of azo dye Orange II in aqueous solutions using copper-impregnated titania

During dyeing process, industries consume large quantity of water and subsequently produce large volume of wastewater. This wastewater is rich in color and contains different dyes. Orange II is one of them. In this article, metal-impregnated TiO₂ P-25 catalyst was used to enhance the photocatalytic degradation of Orange II dye. Photodegradation percentage was followed spectrophotometrically by the measurements of absorbance at λ _max = 483 nm. The effect of copper-impregnated TiO₂ P-25 photocatalyst for the degradation of Orange II has been investigated in terms of percentage removal of color, chemical oxygen demand (COD) and total organic carbon (TOC). As such 98 % color removal efficiency, 97 % percentage removal of COD and 89 % percentage removal of TOC was achieved with TiO₂ P-25/Cu catalysts under typical conditions. Copper-impregnated TiO₂ P-25 photocatalyst showed comparatively higher activity than UV/H₂O₂ homogeneous photodegradation. The relative electrical energy consumption for photocatalytic degradation was considerably lower with TiO₂ P-25/Cu photocatalyst than that with homogeneous photodegradation. Transmission electron microscopic analysis was used for catalyst characterization.     

Daily variations ofE andF region drifts over Tiruchirapalli

Daily variations of the E-W and N-S components of ionospheric drifts, measured using closely spaced receiver method over Tiruchirapalli near magnetic equator, are described for the periods 1973–75 forE region and 1974–75 forF region. N-S component is not observed except for a few occasions during summer months for theE region. E-W component is generally eastward during night hours and westward during day hours. There is a large day-to-day variability in the magnitude as well as in the evening reversal time. Daily variations primarily consist of the diurnal component with average amplitude of about 90 m/sec forF region and of about 80 m/sec forE region. The drift speeds are found to decrease with magnetic activity. It is concluded that the abnormal electrojet region extends upto north of Tiruchirapalli in the Indian zone and drifts here can be used as a monitor of electric field.     

The COVID-19 pandemic and global environmental change: Emerging research needs

The outbreak of COVID-19 raised numerous questions on the interactions between the occurrence of new infections, the environment, climate and health. The European Union requested the H2020 HERA project which aims at setting priorities in research on environment, climate and health, to identify relevant research needs regarding Covid-19. The emergence and spread of SARS-CoV-2 appears to be related to urbanization, habitat destruction, live animal trade, intensive livestock farming and global travel. The contribution of climate and air pollution requires additional studies. Importantly, the severity of COVID-19 depends on the interactions between the viral infection, ageing and chronic diseases such as metabolic, respiratory and cardiovascular diseases and obesity which are themselves influenced by environmental stressors. The mechanisms of these interactions deserve additional scrutiny. Both the pandemic and the social response to the disease have elicited an array of behavioural and societal changes that may remain long after the pandemic and that may have long term health effects including on mental health. Recovery plans are currently being discussed or implemented and the environmental and health impacts of those plans are not clearly foreseen. Clearly, COVID-19 will have a long-lasting impact on the environmental health field and will open new research perspectives and policy needs.     

Societal application of geophysics as an aid to a rescue operation at Jaipur

Geophysical techniques are normally employed to explore the subsurface and determine the anomalies but they are always much larger in dimension. However, a very precise measurement was needed to pin point a misaligned horizontal tunnel made at 47 m depth to intercept an old bore well and rescue a four year child fallen accidently on 9^th November at Jagatpura village in Jaipur, India. Due to the greater depth and presence of iron casing, electric power, etc., no equipment could work to determine the orientation of the tunnel that has lost its direction. The dimension of the site and many other constrains didn’t allow conventional geophysical methods to be used. An innovative method was then designed and successfully applied, which determined 1.92 m deviation of the tunnel end point to the east from the old bore well. The digging was redirected accordingly and the bore well was intercepted. This paper describes the theoretical background, forward simulations and the field experiment of the newly designed geophysical method.     

Investigating spatial contribution of land use types and land slope classes on soil erosion distribution under tropical environment

Natural Hazards - Increasing land occupancy for farming without conservation principles, particularly in the Indonesian island of Java, is resulting in a severe erosion problem. This study...