Genetic diversity and metal tolerance of two marine species: a comparison between populations from contaminated and reference sites

Long-term contamination of the marine environment surrounding a lead smelter offered a unique opportunity to examine how pollutants might have acted to alter genetic characteristics of populations of organisms in the receiving system. This study used random amplified polymorphic DNA analysis to compare the genetic diversity of populations of the prawn, Leander intermedius, and the isopod, Platynympha longicaudata from the smelter discharge site with reference populations. The genetic diversity of the prawn population from the smelter discharge site (Port Pirie) was lower than that found in one reference population, and not significantly different from the other two reference populations. Genetic diversity of the Port Pirie population of isopods was found to be significantly lower than that of all reference populations. Prawns and isopods were also exposed to metal mixtures in the laboratory in a similar ratio to that found in seston near the smelter effluent discharge site. Both pre-exposed and reference populations of prawns tolerated elevated levels of metals and exhibited no significant difference in response. This contrasted with the isopods, with the pre-exposed isopod population showing greater tolerance to elevated metal levels compared with the reference population. These results highlight the need to include a number of reference populations for comparative purposes in genetic diversity studies, and the need to assess the influence of pollution on the genetic diversity of more than one species if genetic diversity analyses are to be used to gauge remediation success.     

Characteristics of alkenone distributions in suspended and sinking particles in the northwestern North Pacific

We investigated alkenones recorded in suspended particles and a settling particle time series collected at three stations, 40N (40°N, 165°E), KNOT (44°N, 155°E), and 50N (50°N, 165°E), in the northwestern North Pacific from December 1997 to May 1999. Emiliania huxleyi, the most abundant alkenone producer in this area, is present in surface to subsurface (to ∼50 m depth) waters. The alkenone concentrations recorded in the suspended particles indicated that the seasonal alkenone particle distribution differed significantly interannually. Alkenone export fluxes at the three sediment-trap stations ranged from 0.16 to 49.3 μg m⁻² day⁻¹, and the maximum export flux, which occurred in summer to fall (July-November), was associated with a high organic carbon export flux. The amount of alkenone produced during the maximum export season accounted for 60-80% of the total annual amount of alkenone, and the alkenones accumulated in the sediment below the traps had characteristics corresponding to subsurface waters during the summer-autumn season. Alkenone-derived temperatures recorded in suspended particles corresponded to the in situ temperature within ∼2 °C. Although alkenone-derived temperatures corresponded approximately to the temperatures observed in the stratified subsurface waters at the three trap stations during the high-export season, large differences were observed during the low-export (winter-spring) period. For example, the alkenone-derived temperatures observed at stations KNOT and 50N were much higher than the in situ subsurface temperatures reported in the World Ocean Atlas 2001. Relatively large differences between alkenone-derived temperatures and in situ temperatures in the subarctic might be due to (1) a low-light limitation or (2) contributions of allochthonous alkenones in particulate material transported from subtropical areas within a warm-core ring.     

Thresholds of Adverse Effects of Macroalgal Abundance and Sediment Organic Matter on Benthic Habitat Quality in Estuarine Intertidal Flats

Confidence in the use of macroalgae as an indicator of estuarine eutrophication is limited by the lack of quantitative data on the thresholds of its adverse effects on benthic habitat quality. In the present study, we utilized sediment profile imagery (SPI) to identify thresholds of adverse effects of macroalgal biomass, sediment organic carbon (% OC) and sediment nitrogen (% N) concentrations on the apparent Redox Potential Discontinuity (aRPD), the depth that marks the boundary between oxic near-surface sediment and the underlying suboxic or anoxic sediment. At 16 sites in eight California estuaries, SPI, macroalgal biomass, sediment percent fines, % OC, and % N were analyzed at 20 locations along an intertidal transect. Classification and Regression Tree (CART) analysis was used to identify step thresholds associated with a transition from "reference" or natural background levels of macroalgae, defined as that range in which no effect on aRPD was detected. Ranges of 3–15 g dw macroalgae m^?2, 0.4–0.7 % OC and 0.05–0.07 % N were identified as transition zones from reference conditions across these estuaries. Piecewise regression analysis was used to identify exhaustion thresholds, defined as a region along the stress–response curve where severe adverse effects occur; levels of 175 g dw macroalgae m^?2, 1.1 % OC and 0.1 % N were identified as thresholds associated with a shallowing of aRPD to near zero depths. As an indicator of ecosystem condition, shallow aRPD has been related to reduced volume and quality for benthic infauna and alteration in community structure. These effects have been linked to reduced availability of forage for fish, birds and other invertebrates, as well as to undesirable changes in biogeochemical cycling.     

Overestimation of short-term coastal cliff retreat rates in the eastern Mediterranean resolved with a sediment budget approach

Our understanding of sea-cliff erosion processes and their response to recent and/or projected environmental changes such as sea-level rise, climate change and anthropogenic development hinges on our ability to quantify sea-cliff retreat rates and their variability through time. Here, we focus on Israel's Mediterranean ‘Sharon’ sea-cliff as a case study for examining the significance of recent short-term (i.e. annual to decadal) cliff-top retreat rates that appear to exceed longer-term rates of ‘background’ (i.e. centennial to millennial) retreat by 1–2 orders of magnitude. We demonstrate that an inherent sampling bias in rate estimates inferred from observation intervals shorter than process episodicity can also explain such a pattern. This potential ambiguity leads to a striking paradox where despite highly accurate and robust documentation of recent cliff-top retreat, such as that obtained from aerial photographs and/or instrumental surveys, the short-term retreat rates of episodically retreating sea cliffs remain poorly constrained. To address this key data gap along the Sharon sea cliff we employed a sediment budget approach that focuses on quantifying the continuous wave scouring of cliff-collapsed material from the shore platform as a rate-limiting process for episodic retreat of the cliff above. We used four high-resolution (0.5 m/pixel) airborne LiDAR data sets acquired between 2006 and 2015 to determine short-term maximum retreat rates of up to ~0.08 m/yr during this nine-year period. These modern retreat rates compare to the cliff's background retreat rate of 0.03 to 0.09 m/yr since the mid-Holocene, as determined herein from multiple geologic and archeological observations. Our results demonstrate that previously reported twentieth century cliff-top retreat rates for this sea cliff, which range up to values of several meters per year, are biased and that sea-cliff erosion rates have not yet been significantly impacted by recent environmental changes in the eastern Mediterranean basin, such as the restriction of sediment supply following emplacement of the Nile's Aswan dam system. © 2018 John Wiley & Sons, Ltd.