几种鲆鲽鱼类精子超微结构及生理特性的比较研究

借助透射电镜分别对石鲽(Kareius bicoloratus)、大菱鲆(Scophthalmus maximus L.)和牙鲆(Paralich- thys olivaceus)成熟精子的超微结构进行了比较研究,进一步测定了石鲽和牙鲆精子的基本生理特性,并研究了17℃和4℃保存精子的活力变化。结果显示3种鱼类精子均为有鞭毛精子,无顶体,尾部轴丝9+2型。不同之处包括:成熟精子的核致密度不同,以石鲽最高,大菱鲆和牙鲆则较低;在大菱鲆和牙鲆精子头部,核前端侧面都有一缺刻状结构,石鲽精子未发现此结构;头部核膜与质膜之间的细胞质含量以大菱鲆的最多;精子中段的线粒体数量不同,大菱鲆较多(10个左右),石鲽次之(8个左右),牙鲆最少,仅为5个左右;中段植入窝深度不同,以大菱鲆陷入核中最深,其他2种比较接近。石鲽和牙鲆精液的pH值比较接近。精子密度以牙鲆较高,为10.18×10~9个/mL±3.37×10~9个/mL。牙鲆和石鲽精子活力的保存时间在17℃下分别为48 h和36 h,4℃下分别为7 d和8 d。精子活力完全失去之前,随着保存时间的延长,精子的激活率和寿命逐渐下降,而涡动时间则相差不大。     

福建东山岛附近海域造礁石珊瑚共生藻的分子系统分类和遗传多样性研究

本研究利用核糖体大亚基5'端序列PCR-RFLP以及转录单元内间隔区(Internal TranlsclqIbed Sppacer,rrS)序列分析相结合的方法,首次对福建东山岛附近海域3种优势种类造礁石珊瑚共生藻进行了分子系统分类和遗传多样性研究.PCR-RFLP分析发现东山岛附近海域3种优势种类造礁石珊瑚共生藻都属于C系群共生藻,而ITS序列的序列分析结果表明东山岛附近海域3种优势种类造礁石珊瑚共生藻都属于C1亚系群.研究结果表明ITS序列进化速度快,适合于造礁石珊瑚共生藻属亚系群水平的鉴定.而东山岛附近海域造礁石珊瑚共生藻的多样性低,暗示东山岛附近海域造礁石珊瑚共生藻共生系统面对外界环境压力的适应能力较低.     

Summer fast ice evolution off Zhongshan Station,Antarctica

Based on the field data acquired in the program of fast ice observation off Zhongshan Station, Prydz Bay, East Antarctica during the austral summer 2005/ 2006, physical properties evolution of fast ice during the ice ablation season is analyzed in detail. Results show that the annual maximum ice thickness in 2005 occurred in later November, and then ice started to reek, and the ablation duration was 62 days; sea water under the ice became warmer synchronously; corresponding to the warming sea ice temperature, a ＂relative cold mid-layer＂ appeared in sea ice; the fast ice marginal line recoiled back to the shore observably, and the recoil distance was 20.9 km from 18 December 2005 through 14 January 2006. In addition, based on the data of sea ice thickness survey along the investigation course of MV Xuelong on December 18 of 2005, the ice thickness distribution paten in the marginal ice zone have been described ： sea ice thickness increased, but the diversity of floe ice thick-ness decreased from open water to fast ice zone distinctly.     

Numerical simulation research on multi-electrodes resistivity imaging survey array

Multi-electrodes Resistivity Imaging Survey （MRIS） is an array method of electrical survey. In practice how to choose a reasonable array is the key to get reliable survey results. Based on four methods of MRIS such as Wenner, Schlumberger, Pole-pole and Dipole-dipole the authors established the model, by studying the result of the forward numerical simulation modeling and inverse modeling, and analyzed the differences among the different forms of detection devices.     

测绘生产计划信息一体化管理的解决方案

分析了测绘生产计划制定与管理中所涉及的各类信息的特点及相互关系,提出了一种简单实用的空间数据与属性数据一体化管理的解决方案.通过建立空间数据表和编制空间数据引擎模块,解决了测绘计划存储、下达、上报和管理过程中相关数据的安全性问题;在SQL Anywhere关系数据库中实现了空间数据与属性数据一体化管理.该方案成功应用于测绘生产计划管理系统中.     

数字化测绘生产管理有关问题研究

数字化测绘生产的特点,带来了生产管理的复杂性和特殊性。数据生产的管理控制,需要把握好顾客需求、技术设计、生产计划、调度控制、质量管理等关键的环节,处理好生产过程中产生的相关问题。     

基于旅游开发影响的五台山植被景观特征分析

以往旅游对植被影响的评价成果较多,但是旅游影响下植被景观与地理因子之间的关系报道较少。本文利用多种数量生态学方法探讨了五台山旅游开发与植被景观之间的生态关系。结果表明：① 旅游开发影响下五台山植被景观可分为单优乔木区、草本区、差级乔草区、中级乔草区、中级灌草区、良级灌草区、良级乔灌草区、优级乔灌草区、以及寺庙区、商业区或居民区9种类型。② 在除趋势对应分析中,第一轴与所有因子的相关性都较大,第二轴与旅游干扰度和海拔呈显著的负相关性。各植被景观类型的空间分布在一定程度上取决于关键性指标的空间格局。③ 除趋势典范对应分析方法能够大大提高植被景观特征与地理因子间的相关性,并将其直观地表达于排序图中。在植被景观特征与地理因子的关系上,第一轴上的相关系数是0.858_DCCA>0.828_DCA,第二轴上的相关系数是0.583_DCCA>0.501_DCA,这说明除趋势典范对应分析方法更优。     

Offspring of aged mothers of rotifer Brachionus calyciflorus shows lower sexual propensity than their elder siblings under crowded conditions

Maternal effects are one of the most interesting topics in evolutionary ecology as they can affect the rate of evolution and population dynamics by phenotypic manipulation of offspring related to fitness.At present,studies examining the interaction between maternal environment effects and maternal age(birth order)effects are scarce.We designed an experiment to reveal whether environmentally induced maternal manipulation works equally on offspring across birth orders in rotifer Brachionus calyciflorus.In the experiment,newborn amictic mothers(FO)were cultured parallelly in low(LD)and high(HD)population density.Offspring(F_1)of young(YF_0)and old(OF_0)mothers in both LD and HD groups were cultured under a crowded condition and the mixis ratios of both F_1 and F_2 were examined.Results show that F_1 from HD-YF_0 had a significantly higher mixis ratio and those amictic ones produced daughters(F_2)with higher mixis ratio in response to crowding than those from HD-OF_0.In contrast,no such differences among F_1 siblings were found in the LD-F_0 group.Therefore,the effect of maternal age on the sexual propensity of the offspring can be affected independently by the maternal population density.     

氨氮急性攻毒对水产经济动物棘胸蛙(Paa spinosa)蝌蚪死亡率、排氨率、耗氧率及窒息点的影响

以体长(1.932±0.204)cm、体质量(1.386±0.055)g的棘胸蛙蝌蚪为实验动物,在水温(24±0.2)℃、DO(7.30±0.01)mg/L、pH 7.30±0.01条件下,采用静水停食法开展了氨氮对蝌蚪的急性攻毒实验,并以此为基础,测定了不同氨氮质量浓度胁迫下棘胸蛙蝌蚪的排氨率、耗氧率、氧氮比及窒息点。结果表明:(1)蝌蚪对氨氮急性攻毒具明显的运动避毒行为,濒死个体的背部皮肤和肝脏均具明显的色变症状;(2)氨氮对蝌蚪的急性致死率具明显的剂量—时间效应,24h、48h、72h、96h LC50依次为177.5、151.7、148.6和146.8mg/L,毒性时段蓄积程度系数呈持续下降趋势,24—48h、48—72h、72—96h的时段MAC值分别为84.04%、10.1%和5.86%;(3)蝌蚪夜均、昼均、日均及时段排氨率随氨氮质量浓度增加均呈阶梯式下降趋势,其中时段排氨率与对照组均无显著差异的为9.80mg/L实验组,夜均、昼均、日均及时段排氨率与对照组均无显著差异的仅为2.45mg/L实验组(P0.05);(4)氨氮对蝌蚪呼吸耗氧具低毒兴奋效应,其夜均、昼均和日均耗氧率随氨氮质量浓度增加均呈先升后降趋势,峰值氨氮质量浓度范围均为4.90—7.35mg/L(P0.05),与对照组均无显著差异的仅为14.70mg/L实验组(P0.05),窒息点含氧量随氨氮质量浓度增加呈先降后升趋势,谷底出现于4.90mg/L实验组(P0.05),与对照组无显著差异的仅为9.80mg/L实验组(P0.05);(5)实验期间,蝌蚪氧氮比(O:N)波动于18.87—25.34之间,昼均和日均氧氮比(O:N)随氨氮质量浓度增加均依次呈"上升—稳定—下降—再稳定"之趋势,两者的峰值氨氮质量浓度范围分别为4.90—14.70mg/L和4.90—9.80mg/L,夜均氧氮比(O:N)呈先升后降趋势,峰值出现于7.35mg/L实验组(P0.05)。