Variation in Water Masses on Shelf of Northern Bering Sea in September 2016-2018

Abstract

Based on hydrological data obtained during the 7th to 9th Chinese National Arctic Research Expeditions in the summers of 2016–2018, the main water structure on the shelf of the northern Bering Sea and the volume and heat fluxes of the Bering Strait throughflow were analyzed. Distinct variability was identified in the three Pacific water masses feeding the strait - Anadyr Water (AW), Bering Shelf Water (BSW) and Alaskan coastal water (ACW). Overall, the temperature and salinity of the entire section increased each year, with 2018 showing significant anomalies, i.e., a temperature anomaly of up to 1?°C and a maximum salinity anomaly of 2. From 2016 to 2018, the extent of the ACW gradually narrowed in the eastern part of this section, while the AW expanded eastward each year. The net volume transport through each of the three sections increased poleward from 1.65?Sv to 2.76?Sv, with the AW increasing from 0?Sv to 1.03?Sv, the BSW varying between 0.52–1.65?Sv, and the ACW gradually decreasing from 1.04?Sv to disappearing completely. The net heat fluxes were also poleward, varying between 25.77 TW and 61.50 TW, and showing a significant increase. Significant variations in magnitude and extent were observed in each water mass of the Bering Strait throughflow, which could produce widespread effects in the Arctic Ocean and the global ocean beyond.     

东太平洋赤道海域茎柔鱼小型群体资源分布及其渔场环境特征

茎柔鱼(Dosidicus gigas)是我国远洋渔业的重要捕捞对象。当前针对茎柔鱼渔场分布及其与环境关系的研究多集中于秘鲁海域,针对赤道海域茎柔鱼特定种群小型群体资源分布及其渔场环境特征研究较少。根据2019年12月至2020年2月茎柔鱼生物学数据,2019年12月至2020年4月生产和环境数据,运用胴长-体重关系拟合、地统计插值、广义可加模型(GAM)探究其资源分布及渔场环境状况。结果表明：东太平洋赤道海域茎柔鱼胴长范围为136~407 mm,体重范围为117~1557 g；2019年12月至2020年4月各月渔获量呈先增加后减小趋势,2月渔获量最高；CPUE曲线除2月增加外,总体呈下降趋势；渔场集中分布于0°~3°S、105°W~114°W海域,不同月份渔场重心经向变化明显；渔场最适SST范围是24.5~25.5 °C,最适Chl-a范围是0.16~0.20 mg/m3,月份是影响茎柔鱼CPUE的主要因子。研究表明：该海域茎柔鱼渔获主要为小型群体；小型群体生长发育期(2–3月)对渔场分布有重要影响,生长发育期前茎柔鱼集群度高,生长发育期后逐渐分散活动；单一影响因子与茎柔鱼CPUE相关性不显著,综合考虑其他环境因素及其交互影响是今后的研究方向。     

Development of a real-time PCR method for Thalassiosira rotula rapid detection

Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.     

Seasonal and annual variations of marine sinking particulate flux during 1993~1996 in the central South China Sea

A total of 67 samples from the upper and lower sediment traps in the central South China Sea were analyzed, which were collected during 1993~1996. It is indicated that the distribution of stable isotope values, surface primary productivity, fluxes of total particulate matter, carbonate, biogenic opal, organic carbon, planktonic foraminiferal species and their total amount exhibit obviously seasonal and annual fluctuations. High values of the fluxes occurred in the prevailing periods of the northeastern and southwestern monsoons, and the low values occurred during the periods between the two monsoons. The fluxes of some planktonic foraminiferal species (Globigerinoides sacculifer, G. ruber, Globigerinita glutinata, Neogloboquadrina dutertrei) and their percentages also exhibit two prominent peaks during the prevailing periods of the northeastern and southwestern monsoons respectively, while those of Globigerina bulloides, Globorotalia menardii and Pulleniatina obliquiloculata only exhibit one peak in the prevailing periods of the northeastern monsoon. In addition, fluxes and percentages of Globigerinoides sacculifer and Globorotalia menardii as well as the fluxes of carbonate and total amount of planktonic foraminifera decrease gradually from 1993 to 1996, and those of Globigerina bulloides, Globigerinita glutinata and biogenic opal increase gradually from 1993 to 1996. The fluxes of carbonate and organic carbon in the upper trap are higher than those in the lower one. The study indicates that the seasonal and annual variations of the sediment fluxes and planktonic foraminiferal species are mainly controlled by the changes of surface primary productivity and hydrological conditions related to the East Asian monsoon. The lower carbonate and organic carbon fluxes in the lower trap are related to the dissolution.     

Resources calculation of cobalt-rich crusts with the grid subdivision and integral method

On the basis of three geological models and several orebody boundaries,a method of grid subdivision and integral has been proposed to calculate and evaluate the resources of cobalt-rich crusts on the seamounts in the central Pacific Ocean.The formulas of this method are deduced and the interface of program module is designed.The method is carried out in the software "Auto mapping system of submarine topography and geomorphology MBChart".This method and program will possibly become a potential tool to calculate the resources of seamounts and determine the target diggings for China's next Five-year Plan.     

南大洋普里兹湾基于234Th/238U不平衡法的POC输出通量研究

中国第22次南极科学考察(2005年11月至2006年3月)期间,测定了南极普里兹湾海域5个站位的从表层至150 m水深的不同层位水样中溶解态和颗粒态234Th,238U的放射性比活度以及颗粒有机碳.利用234Th/238U在上层水体中的不平衡,计算了南极普里兹湾上层水体中234Th的平均停留时间和输出通量.结果显示,随着纬度的增加,上层水体中颗粒态和溶解态234Th的平均停留时间总体趋向减小,并在中纬度站位出现了最低值,分别为1~8和29~48 d,而颗粒态和溶解态234Th的输出通量则在中纬度站位出现了最大值,分别为21~38和26~39 dpm/(m3·d).运用箱型清除模式,利用两种不同的方法估算了各水柱中从真光层底部输出的POC通量,平均值分别达到104.7 mmol/(m2·d)(E法)和120.6 mmol/(m2·d)(B法),表明南极普里兹湾夏季存在很高的新生产力,它将会对该海域碳的生物泵过程产生重要作用.     

乳糖诱导重组别藻蓝蛋白(rAPC)在大肠杆菌(Escherichia coli)中的表达

采用廉价的乳糖替代IPTG诱导重组别藻蓝蛋白(rAPC)在大肠杆菌(Escherichiacoli)JM109中表达,对诱导产物表达的培养基、培养条件、诱导剂的浓度和诱导时机进行了优化,将优化条件用于5L自控发酵罐进行高密度培养。结果表明,对rAPC表达的条件进行优化后,乳糖诱导rAPC的表达率可达26.8%,与IPTG的诱导结果接近;在高密度培养中,OD600最大达21.8。研究结果可为乳糖替代IPTG大规模生产rAPC奠定基础。     

Study on copper complexing ligand concentrations in several China’s coastal waters

Copper complexing ligand concentrations in the Daya Bay, Qingdao coast, Jiaozhou Bay, South China Sea and Huanghe Estuary waters were determined by the anodic stripping voltammetry technique. The distribution regularity and the relationship with other parameters were discussed. The results were as follows: Copper complexing ligand concentrations of the South China Sea were a little higher than those of other sea areas, and they were apparently higher than those of the ocean. Compared with the subsurface layer (SSL) in the sea surface microlayer copper complexing ligand concentrations showed an enrichment phenomenon, of which the mechanism is similar to dissolved organic matter. The metal complexing ligand concentration profiles of the South China Sea showed that the value in the sea surface was the highest, then it decreased with depth accruing, and a higher value appeared at the bottom. Copper complexing ligand concentrations were higher than those of cadmium and lead. Ligands in each sea area exhibited a complicated property. In short, the distribution regularity of copper complexing ligand concentrations in China' s coastal waters was consistent with that of other regions in the world. Meanwhile, the positive relationship between the copper complexing ligand concentrations and biological oxygen demand, chemical oxygen demand, dissolved organic carbon, and viscosity were found clearly.     

假单胞菌(Pseudomonas sp.cn4902)磷酸甘油磷酸酯酶基因克隆与鉴定

采用随机克隆、功能筛选、逐次排除和同源比较的基因克隆新策略进行克隆假单胞菌(Pseudomonas sp．cn4902)磷酸甘油磷酸酯酶基因的研究。结果表明，该结构基因长819bp，与铜绿假单胞菌的磷酸甘油磷酸酯酶基因的核苷酸一致性达61．5％，氨基酸同源性为56．2％。该基因已输入GenBank数据库，收录号AF348165。将该基因转化大肠杆菌，受体菌在含NaCl 1．0mol／L的培养基中甘油含量升高2．9倍，最终菌浓度提高3．6倍。可见这是一个与生物耐盐性相关的主基因，以其转化、培育耐盐农作物的前景十分光明。     

胶州湾围隔浮游生态系统氮、磷营养盐迁移-转化模型研究

通过对目前生态动力学模型的总结和综合,以生态系统中氮、磷营养盐循环为主线,建立了适用于海洋围隔浮游生态系统的多变量的营养盐迁移-转化动力学模型.该模型包括浮游植物、浮游动物、溶解无机态营养盐、溶解有机态营养盐和生物碎屑5个模块,涉及溶解无机氮、磷酸盐、溶解有机氮、溶解有机磷、浮游植物、浮游动物和生物碎屑7个状态变量.分别利用1999年秋季和2000年夏季胶州湾围隔生态实验数据进行了模型和验证工作,成功地模拟了富加营养盐条件下围隔浮游生态系统中氮、磷营养盐生物化学迁移-转化过程,并确定了20余个参数的量值.