海带雌性原生质体的分离与培养

于1993年1月 - 1993年4月，为了制备遗传性纯一的基因工程受体，用酶法从海带雌配子体单性生殖系分离雌性单倍体原生质体。从皱纹盘鲍消化腺及消化器官提取出鲍酶，在25°C下、1％（W／V）的鲍酶与2％（W／V）的纤维素酶（Onozuka R－10）合用能有效解离单细胞和原生质体。海带雌性原生质体经2d培养再生了细胞壁。     

Preliminary report on radionuclide concentrations in the bottom waters at the entrance of Wakasa Bay with special reference to the Japan Sea Proper Water

Marked fluctuation of concentrations of⁹⁰Sr and¹³⁷Cs was observed in the bottom waters at the entrance of Wakasa Bay during 1987–1992, and the cause was investigated. The concentrations of⁹⁰Sr and¹³⁷Cs in the bottom waters were significantly low when the upper level of the Japan Sea Proper Water (JSPW) was high and covered the sampling depth, but high when the upper level of the JSPW was low. The cause of the fluctuation observed in the bottom waters is, therefore, suggested to be the vertical fluctuation of the upper level of the JSPW on the shelf slope, which has been little described before.     

Carbon Isotope Geochemistry of the Orinoco Basin

Stable carbon isotope ratios have been used to study the sources of particulate organic carbon (POC) and total dissolved inorganic carbon in the Orinoco Basin. The isotopic composition of total dissolved inorganic carbon shows a range of from -8·1 to -23·0 ppt, an indication of dominance of biological processes. The isotopic composition of POC exhibits a range of from -24·1 to -34·6 ppt with little seasonal variation. The isotopic evidence indicates that the POC is predominantly of terrestrial origin rather than a result of in situ planktonic production. The similarity of isotopic composition of POC and coastal sediments suggests that riverine organic detritus has been transported 30-50 km offshore in a direction parallel to the Orinoco river channel.     

Estrogenic responses to xenobiotics in channel catfish (Ictalwus punctatus)

Several environmentally relevant contaminants are characterized as xenoestrogens by virtue of their ability to induce responses similar to 17β-estradiol (E2). There is concern that exposure to these xenoestrogens may result in endocrine and, thus, reproductive disruption. The objective of the present research was to determine whether xenobiotics known to elicit estrogenic responses in mammals were estrogenic in channel catfish, and if these compounds were capable of altering normal physiological response to E2. Sexually immature catfish were exposed by intraperitoneal injection to E2, suspected xenoestrogens or combination doses of E2 and xenoestrogen. Appearance of vitellogenin (Vg) in serum was used as the bioindicator of estrogenicity; Vg was measured by ELISA 7 days after injection. The ED₅₀ of E2 for the appearance of Vg in blood, 0.6 mg/kg, was used as the positive control. Synthetic estrogens, ethinylestradiol, mestranol and DES were effective in inducing Vg; the antiestrogen tamoxifen inhibited response to E2 when given in a combination dose. Treatment by both methoxychlor and p-nonylphenol resulted in Vg appearance in serum; the doses required were 300 times that of E2, and the vitellogenic response was less when compared to the E2-induced response. In addition, a combination dose of methoxychlor with E2 decreased the magnitude of the response to E2. Other mammalian xenoestrogens, o,p′-DDT, chlordecone, lindane and β-HCH, were not able to produce a vitellogenic response in this study.     

Three-dimensional visualization of mission planning and control forthe NPS autonomous underwater vehicle

The Naval Postgraduate School (NPS) is constructing a small autonomous underwater vehicle (AUV) with an onboard mission control computer. The mission controller software for this vehicle is a knowledge-based artificial intelligence (AI) system requiring thorough analysis and testing before the AUV is operational. The manner in which rapid prototyping of this software has been demonstrated by developing a controller code on a LISP machine and using an Ethernet link with a graphics workstation to simulate the controller's environment is discussed. The development of a testing simulator using a knowledge engineering environment (KEE) expert system shell that examines AUV controller subsystems and vehicle models before integrating them with the full AUV for its test environment missions is discussed. This AUV simulator utilizes an interactive mission planning control console and is fully autonomous once initial parameters are selected     

Properties of structure and function of cadmium-binding proteins/peptides from Euglena gracilis

Euglena gracilis makes two small Cd-binding proteins/peptides (Cd-BP I and Cd-BP II) in response to exposure to Cd²⁺. These proteins migrate slower than mammalian Cd-metallothionein (Cd-Mt) through Sephadex G-75, but like Cd-Mt also contain Zn and are separated into two species when chromatographed over a DEAE column. Both BPs are much less stable than Cd-Mt under acidic conditions. One source of this instability is that at least Cd-BP II contains acid-labile sulfide co-ordinated to the metals. It has been quantitated in Cd-BP II and shown to have a value of 1·25 ± 0·10 S²⁻/Cd. The reactivity of the Cd-binding sites in ligand substitution and thioldisulfide exchange reactions has been assessed. The reactions of Cd-BP I and II with pyridylazoresorcinol (PAR) were slow and displayed complex kinetic behavior. Similarly, both Cd-BPs react slowly and with complicated kinetics with 5,5′-dithiobis-(2-nitrobenzoate).     

Trace metal concentrations in sediments of the Eastern Mississippi Bight

Sediments collected from 3 onshore-offshore transects (12 stations total) in the Eastern Mississippi Bight at 5 different times during 1987–1988 have been analyzed for total Ag, As, Ba, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Sb, Se, Sn, Th and Zn. The data show considerable variation both spatially and temporally, largely as a result of natural variability in grain size and mineralogy. Clay-rich samples from deep water were always more metal-rich than sandy samples from shallow areas. Many samples from near the Mississippi River Delta appeared to be enriched in Ba by about a factor of two, probably as a result of contamination from oil well drilling mud, but there were few other indications of pollution influences. Many samples from throughout the area gave Mn/Fe and Cd/Fe ratios a factor of two or more lower than their Mississippi River source material. This shows that biochemical activity in the sediment is capable of solubilizing reducible and adsorbed metals.     

The depletion of oil

The trend of previous estimates of the world's ultimate recovery of oil suggests a figure of 1650 BBO, of which 1438 BBO have been discovered and 978 BBO remain to be produced. Some large reported reserve figures are questioned. Importance is attached to the mid-point of depletion when rising production tends to give way to decline. The share of the world's supply coming from six Middle East countries is set to continue to rise from its 1986 low of 16% to as much as 50% by 2010, with the likely consequence of higher oil prices and serious political implications. The world is rapidly approaching a turning point when it has to face the consequences of an irreversible decline in oil production.