Methoxychlor bioremediation by defined consortium of environmental Streptomyces strains

Methoxychlor is an organochlorine pesticide used worldwide against several insect pests, resulting in human exposure. This pesticide mimics endocrine hormone functions, interfering with normal endocrine activity in humans and wildlife. For this reason, it is imperative to develop methods to remove this pesticide from the environment, and though, bioremediation using microorganisms results as an excellent strategy. Five Streptomyces spp. strains previously isolated from organochlorine-polluted sites and capable to grow and remove methoxychlor were combined as different mixed cultures to increase methoxychlor removal. From the 39 consortia tested, one consortium (Streptomyces spp. A6, A12, A14, M7) was selected because of its high pesticide removal and specific dechlorinase activity to be assayed on slurry and soil systems. This consortium showed higher biomass values (8.3 × 10⁶ ± 5.7 × 10⁵ CFU mL^?1) and methoxychlor removal (56.2 ± 2.3 %) on enriched slurry than in non-enriched slurry (7.3 × 10⁵ ± 1.2 × 10⁵ CFU mL^?1 and 45.6 ± 7.4 % of pesticide removal). In soil systems, Streptomyces consortium showed higher growth (1.0 × 10¹¹ ± 5.0 × 10¹⁰ CFU g^?1) than in enriched slurry, although differences in methoxychlor removal between both culture conditions were not statistically significant. Therefore, the selected Streptomyces consortium may be suitable for the development of in situ (soil) and ex situ (slurry bioreactor) bioremediation methods because of their potential to remove methoxychlor from different systems.     

Out‐of‐plane seismic behaviour of rocking masonry walls

The evaluation of the out‐of‐plane behaviour of unreinforced walls is one of the most debated topics in the seismic assessment of existing masonry buildings. The discontinuous nature of masonry and its interaction with the remainder of the building make the dynamic modelling of out‐of‐plane response troublesome. In this paper, the results of a shaking table laboratory campaign on a tuff masonry, natural scale, U‐shaped assemblage (façade adjacent to transverse walls) are presented. The tests, excited by scaled natural accelerograms, replicate the behaviour of external walls in existing masonry buildings, from the beginning of rocking motion to overturning. Two approaches have been developed for modelling the out‐of‐plane seismic behaviour: the discrete element method and an SDOF analytic model. Both approaches are shown to be capable of reproducing the experimental behaviour in terms of maximum rotation and time history dynamic response. Finally, test results and numerical time history simulations have been compared with the Italian seismic code assessment procedures. Copyright © 2011 John Wiley & Sons, Ltd.     

Sponge spicules indicate Holocene environmental changes on the Nabileque River floodplain, southern Pantanal, Brazil

Sponge spicules are siliceous microfossils that are especially useful for analysis of sandy fluvio-lacustrine sediments. Sponge spicules in a long sediment core (~550 cm below surface), consisting of fine sand, sandy silt, and organic-rich mud, recovered from the floodplain of the Nabileque River, southern Pantanal, Brazil (S20°16′38.3″/W57°33′00.0″), form the basis of a novel paleoenvironmental interpretation for this region. Optically stimulated luminescence dates constrain the timing of deposition to the middle-late Holocene and all spicules identified are typical of the Brazilian cerrado biome. The base of the section is dominated by Oncosclera navicella Carter 1881, Metania spinata Carter 1881, and Corvospongilla seckti Bonetto and Ezcurra de Drago 1966, which indicate a lotic to semi-lotic environment strongly influenced by an actively meandering river channel at ~6.7–5.7 ka BP. The appearance of Heterorotula fistula Volkmer-Ribeiro and Motta 1995, Dosilia pydanieli Volkmer-Ribeiro 1992 and Radiospongilla amazonensis Volkmer-Ribeiro and Maciel 1983 at ~340 cm downcore suggests a reduction in flowing water and a more stable lentic environment, consistent with deposition in an oxbow lake. This oxbow lake environment existed during an interval of regional aridity between ~4.5 and 3.9 ka BP. Spicules, as well as phytoliths and diatoms, are highly variable moving up-section, with species from both lotic and lentic ecosystems present. Above ~193 cm, the total abundance of spicules declines, consistent with wetter climate conditions and development of an underfit river similar to the modern floodplain. Results support hypotheses related to migration of the Paraguay River inferred from geomorphological studies and add a key southern-region dataset to the emerging Holocene database of paleoenvironmental records from the Pantanal wetlands.     

Thermal expansion and dehydroxylation of phengite micas

Phengite samples (2M ₁ and 3T politypes) and a synthetic end-member muscovite specimen were studied by in situ high-temperature synchrotron radiation X-ray diffraction. The measured volume thermal expansion of 2M ₁ phengite (<α _V> ≈ 36.6 × 10⁻⁶ K⁻¹) was systematically greater than <α _V> of the 3T polytype (≈33.3 × 10⁻⁶ K⁻¹). A positive linear correlation between the average thermal expansion on (001) plane and the mean tetrahedral rotation angle at ambient condition is proposed on the ground of new measurements and literature data. Dehydroxylation processes were observed in 2M ₁, starting at 1,000 K in 3T at 800 and 945 K in synthetic muscovite. Rietveld refinements allowed a determination of structural variations upon heating of phengite samples and their dehydroxylate phases. The phengite structure expands by regularizing the tetrahedral sheet and by reducing the bond length differences between the outer and inner coordination shell of the interlayer site. The dehydroxylate phase derived from 2M ₁ is characterized by fivefold polyhedra in the low temperature form as a consequence of two OH groups reacting to form H₂O + O (residual). The dehydroxylate exhibits an increase of the cation–cation distances along the M–Or–M bonds with respect to low-temperature phengite structures. For the 3T phase, we were unable to achieve completion of dehydroxylation. The refined structural model of the dehydroxylate phase shows two hydroxyl sites, but at a short distance from one another. This result suggests that the dehydroxylation reaction did not proceed to completion. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users.     

A model for the formation of vesiculated tuff by the coalescence of accretionary lapilli

Observations on phreatomagmatic ash deposits of Phlegraean Fields and Vesuvius supply evidence for the origin of vesiculated tuff in a cool environment. Early deposition by fallout of a matrix-free bed of damp accretionary lapilli is followed by deposition of cohesive mud or a mud rain. The lapilli bed becomes partly or completely transformed into a vesiculated tuff by mud percolation and eventual coalescence of accretionary lapilli with consequent trapping of air originally contained in the interstices. The proposed mechanism accounts for vesiculated tuff formation in distal deposits beyond limits commonly attained by pyroclastic surges. This same mechanism may, nevertheless, also operate in proximal tuff-ring and cone deposits during fallout of phreatomagmatic ash separating bed sets in surge-dominated successions. The sequence of events in the proposed model fits well with the evolution of a cooling phreatomagmatic ash cloud in which early ash aggregation (accretionary lapilli fallout) is followed closely by steam condensation (mud or muddy rainfall). This new model invoking a cool-temperature origin is intended to be complementary to previously proposed theories. Although difficult to assess because of the often complete obliteration of original lapilli, the process is believed to be relatively common in the generasion of vesiculated tuffs within phreatomagmatic deposits.     

Uniform fragility spectra for the performance‐based seismic design of structures considering variabilities in structural properties

This paper presents a new methodology based on structural performance to determine uniform fragility design spectra, i.e., spectra with the same probability of exceedance of a performance level for a given seismic intensity. The design spectra calculated with this methodology provide directly the lateral strength, in terms of yield‐ pseudo‐accelerations, associated with the rate of exceedance of a specific ductility characterizing the performance level for which the structures will be designed. This procedure involves the assessment of the seismic hazard using a large enough number of seismic records of several magnitudes; these records are simulated with an improved empirical Green function method. The statistics of the performance of a single degree of freedom system are obtained using Monte Carlo simulation considering the seismic demand, the fundamental period, and the strength of the structure as uncertain variables. With these results, the conditional probability that a structure exceeds a specific performance level is obtained. The authors consider that the proposed procedure is a significant improvement to others considered in the literature and a useful research tool for the further development of uniform fragility spectra that can be used for the performance‐based seismic design and retrofit of structures.     

Detritus Rolling Down a Vertical Cliff of the Ligurian Sea (Italy): The Écological Role in Hard Bottom Communities

Abstract. The detritus rolling down a vertical cliff was studied at Portofino Promontory (Ligurian Sea, Italy) at 16 m depth (January 1988- January 1989). The large amount of collected sediment is mainly due to the position of the traps; they collected not only the sedimenting material, but also the detritus rolling down along the cliff. The amount of sediment matter is 7 times greater in winter than in summer, showing a good correlation with rough seas and rain that cause a "mechanical cleaning" of animal and plant debris (Sérpulid tubes, shells, exuvias. leaves) along the cliff.
The Total Suspended Matter (TSM) in the water column shows maximum values in March and September. The organic fraction has an average concentration of 0.81 mg 1^-l. The phytoplanktonic biomass, measured as Chi a , shows an annual cycle with a peak in spring (1–2.7μg·l^-1). while in other periods of the year the value is about 0.3-0.5 μg·1^-1. A significant relationship was found between organic detritus in the collected sédiments and the Chi a in the water column. The great influence of the rocky wall detritus on the normal trend of the TSM in the coastal water column is pointed out.