Heterogeneity in mycorrhizal inoculum potential of flood-deposited sediments

Riparian areas are diverse systems where flooding creates new sites for establishment of vegetation. Symbioses with soil microorganisms, such as mycorrhizal fungi, affect vascular plant growth and community composition. It is unknown, however, how mycorrhizal fungi are dispersed along rivers and what potential they have to inoculate roots of plants establishing on recently deposited sedimentary surfaces of flood plains. We measured AMF inocula in sediment deposited by an average spring flood along an expansive riverine flood plain in Montana, USA, to determine whether AMF inocula were present in sediments and what types of propagules (spores, hyphae, or colonized root fragments) contribute to AMF infectivity. Flood-deposited sediments contained sufficient inocula for AMF to colonize host plants (Sorghum sudanense) grown in a greenhouse, and both AMF hyphal lengths and spore densities were correlated with infectivity. Availability of mycorrhizal inocula, which is patchily distributed in this system, may lead to microsites that differ in ability to support establishment and growth of early-successional plants.     

Sand dune movement in the Victoria Valley, Antarctica

We use vertical aerial photographs and LiDAR topographic survey data to estimate dune migration rates in the Victoria Valley dunefield, Antarctica, between 1961 and 2001. Results confirm that the dunes migrated an average of 1.5 m/year. These values are consistent with other estimates of dune migration from cold climate deserts and are significantly lower than estimates from warm deserts. Dune migration rates are retarded by the presence of entrained ice, soil moisture and a reversing wind regime. Dune absorption, merging and limb extension are apparent from the time-series images and account for significant changes in dune form and the field-scale dune pattern. Dune-field pattern analysis shows an overall increase in dune-field organization with an increase in mean dune spacing and a reduction in total crest length and defect density. These data suggest that dunes in other cold desert environments on Earth, Mars or Titan, that may also have inter-bedded frozen laminae, still have the potential to migrate and organize, albeit at lower rates than dunes in warm deserts.     

Post-glacial sediment dynamics in the Irish Sea and sediment wave morphology: Data–model comparisons

The irregular seafloor of the narrow Irish Sea on the NW European Shelf has been documented over several decades. From recently collected swath bathymetry data, very large trochoidal, nearly symmetrical sediment waves are observed in many parts of the Irish Sea and appear similar to those described from other continental shelf seas in North America that were covered by glacigenic sediments during the Last Glacial Maximum. Swath multibeam and single beam bathymetry data, backscatter intensity, shallow seismic imagery, video footage and sediment cores from the Irish Sea high sediment waves have been integrated to identify their genesis with reference to present and past hydrodynamic variability. From cross-sectional profiles over asymmetrical sediment waves in the Irish Sea the direction of asymmetry is used to map residual bed stress directions and associated bedload transport paths. Irish Sea peak bed stress vectors were generated using a two-dimensional palaeo-tidal model for the NW European shelf seas and compare well with the observations. Tidally induced bed stresses are modelled to have increased between 7–10 ka BP, to be nearly symmetrical in magnitude and to have reversed in dominant direction on a millennial scale. These environmental conditions during the post-glacial marine transgression are suggested here to help comprehend the construction of the very large sediment waves, with local variations due to differences in sediment grain size, sediment supply, water depth and intensified currents due to seafloor slopes. Model parameterisation using an open ocean boundary with time-dependent tidal changes and the implementation of high-resolution bathymetric information will improve future models of small-scale bed shear stress patterns and improve the predictive value of such modelling efforts.     

Microbial load from animal feces at a recreational beach

The goal of this study was to quantify the microbial load (enterococci) contributed by the different animals that frequent a beach site. The highest enterococci concentrations were observed in dog feces with average levels of 3.9 × 10⁷ CFU/g; the next highest enterococci levels were observed in birds averaging 3.3 × 10⁵ CFU/g. The lowest measured levels of enterococci were observed in material collected from shrimp fecal mounds (2.0 CFU/g). A comparison of the microbial loads showed that 1 dog fecal event was equivalent to 6940 bird fecal events or 3.2 × 10⁸ shrimp fecal mounds. Comparing animal contributions to previously published numbers for human bather shedding indicates that one adult human swimmer contributes approximately the same microbial load as one bird fecal event. Given the abundance of animals observed on the beach, this study suggests that dogs are the largest contributing animal source of enterococci to the beach site.     

Collection of intact sediment cores with overlying water to study nitrogen- and oxygen-dynamics in regions with seasonal hypoxia

Settled particles of fresh, labile organic matter may be a significant source of oxygen demand and nutrient regeneration in seasonally-hypoxic regions caused by nutrient inputs into stratified coastal zones. Studying the dynamics of this material requires sediment sampling methods that include flocculent organic materials and overlying water (OLW) at or above the sediment–water interface (SWI). A new coring device (“HYPOX” corer) was evaluated for examining nitrogen- (N) and oxygen-dynamics at the SWI and OLW in the northern Gulf of Mexico (NGOMEX). The HYPOX corer consists of a “Coring Head” with a check-valve, a weighted “Drive Unit,” and a “Lander,” constructed from inexpensive components. The corer collected undisturbed sediment cores and OLW from sediments at NGOMEX sampling sites with underlying substrates ranging from sand to dense clay. The HYPOX corer could be deployed in weather conditions similar to those needed for a multi-bottle rosette water-sampling system with 20 L bottles. As an example of corer applicability to NGOMEX issues, NH₄⁺ cycling rates were examined at hypoxic and control sites by isotope dilution experiments. The objective was to determine if N-dynamics in OLW were different from those in the water column. “Ammonium demand,” as reflected by potential NH₄⁺ uptake rates, was higher in OLW than in waters collected from a meter or more above the bottom at both sites, but the pattern was more pronounced at the hypoxia site. By contrast, NH₄⁺ regeneration rates were low in all samples. These preliminary results suggest that heterotrophic activity and oxygen consumption in OLW in the hypoxic region may be regulated by the availability of NH₄⁺, or other reduced N compounds, rather than by the lack of sufficient labile organic carbon.     

Correlations between microbial indicators, pathogens, and environmental factors in a subtropical Estuary

The objective of this study was to evaluate whether indicator microbes and physical–chemical parameters were correlated with pathogens within a tidally influenced Estuary. Measurements included the analysis of physical–chemical parameters (pH, salinity, temperature, and turbidity), measurements of bacterial indicators (enterococci, fecal coliform, Escherichia coli, and total coliform), viral indicators (somatic and MS2 coliphage), viral pathogens (enterovirus by culture), and protozoan pathogens (Cryptosporidium and Giardia). All pathogen results were negative with the exception of one sample which tested positive for culturable reovirus (8.5 MPN/100 L). Notable physical–chemical parameters for this sample included low salinity (<1 ppt) and high water temperature (31 °C). Indicator bacteria and indicator virus levels for this sample were within average values typically measured within the study site and were low in comparison with levels observed in other freshwater environments. Overall results suggest that high levels of bacterial and viral indicators were associated with low salinity sites.     

Wetland buffers: numerical modeling of wave dissipation by vegetation

The resiliency of coastal communities is imperative because these areas experience risk of damage from coastal storms as well as increasing population pressures and development. The severity of this hazard is compounded by sea level rise and a potential increase in storm intensities due to climate change. The ability of coastal communities to plan for, resist, and quickly and completely recover from severe coastal storm events and flooding is of critical importance. There is a growing interest in applying complementary and redundant approaches to reduce the flood risk of these vulnerable communities, such as incorporating natural and nature‐based features into the project planning process. However, accounting for the benefits of these nature‐based features in coastal design is still challenging. One of the natural features generally acknowledged to offer coastal protection benefits is wetlands. Using laboratory experiments of artificial vegetation as a foundation, the bounds of wave dissipation by vegetation are explored analytically and the effectiveness of wave dissipation by vegetation over large scales is investigated using the spectral wave model STWAVE. Wave heights modeled using a vegetation dissipation formulation are compared to those modeled with the current practice of representing vegetation using bottom friction, particularly the Manning formulation. The vegetation dissipation formulation reduced more wave energy than the Manning bottom friction formulation for submerged wetlands. Because the Manning formulation does not integrate vegetation properties, to achieve consistent results would require varying the Manning n coefficient to account for the spatial and temporal variation in form drag induced by the plants due to changes in plant density, diameter, and degree of plant submergence. Thus, a re‐evaluation of existing methods for assessing wave dissipation by vegetation is recommended for wider application of vegetation dissipation formulations in numerical models. Such models are critical for evaluating coastal resiliency of communities protected by wetland features. Published 2016. This article is a U.S. Government work and is in the public domain in the USA.     

Changes in atmospheric circulation and the Arctic Oscillation preserved within a millennial length reconstruction of summer cloud cover from northern Fennoscandia

Cloud cover currently represents the single greatest source of uncertainty in General Circulation Models. Stable carbon isotope ratios (δ¹³C) from tree-rings, in areas of low moisture stress, are likely to be primarily controlled by photosynthetically active radiation (PAR), and therefore should provide a proxy record for cloud cover or sunshine; indeed this association has previously been demonstrated experimentally for Scots pine in Fennoscandia, with sunlight explaining ca 90% of the variance in photosynthesis and temperature only ca 4%. We present a statistically verifiable 1011-year reconstruction of cloud cover from a well replicated, annually-resolved δ¹³C record from Forfjord in coastal northwestern Norway. This reconstruction exhibits considerable variability in cloud cover over the past millennium, including extended sunny periods during the cool seventeenth and eighteenth centuries and warm cloudy periods during the eleventh, early fifteenth and twentieth centuries. We find that while a generally positive relationship persists between sunshine and temperature at high-frequency, at lower (multi-decadal) frequencies the relationship is more often a negative one, with cool periods being sunny (most notably the Little Ice Age period from 1600 to 1750 CE) and warm periods more cloudy (e.g. the mediaeval and the twentieth century). We conclude that these long-term changes may be caused by changes in the dominant circulation mode, likely to be associated with the Arctic Oscillation. There is also strong circumstantial evidence that prolonged periods of high summer cloud cover, with low PAR and probably high precipitation, may be in part responsible for major European famines caused by crop failures.     

Chemical Separation of Tungsten and Other Trace Elements for TIMS Isotope Ratio Measurements Using Organic Acids

One requirement for isotope ratio measurement results with small measurement uncertainties is that the element of interest is effectively separated from the sample matrix. Efficient chemical separation of W from matrix components, especially Ti, can be challenging, particularly for large test portion masses (> 1 g). We present a new W separation procedure that takes advantage of the distinct complexation behaviour of Ti and W with citrate ligand in a moderately low pH, oxidising solution. This preparation procedure can reduce the Ti/W ratio of large (4–10 g) basaltic (i.e., high‐matrix) test portions by a factor of 10⁵, relative to their original compositions, in a two‐step separation procedure. The procedure additionally provides a separate, well‐purified Mo fraction. We show that optimal separation requires precise selection of reagent concentrations and sample load. The procedure was employed to determine the μ¹⁸²W composition of BHVO‐2 as ?6.7 ± 4.2 (2 standard deviation, 2s). The principles derived from this method may prove useful for chemical separation of other elements used for geochemical and cosmochemical applications given an appropriate selection of organic acid. Future successful applications of this method may reveal that the use of organic acids as procedural reagents is a currently under‐utilised tool for efficient chemical separation protocols.