Viruses as potential regulators of regional brown tide blooms caused by the alga,Aureococcus anophagefferens

Blooms of the brown tide organismAureccoccus anophagefferens have recurred in the coastal bays in New Jersey since 1995 and in the coastal bays of Long Island since 1985. Intracellular viral-like particles (VLPs) were documented during 1999–2000 brown tide blooms in Little Egg Harbor, New Jersey, but it was not determined whether cells were infected during the termination of the bloom. The objective of this study was to determine if VLPs infected and lysed natural populations ofA. anophagefferens in coastal bays of New Jersey and New York in 2002 with the same frequency as in 1999–2000 and especially at the termination of the bloom. Our results confirmed that the highest percentage (37.5%) of VLP-infected cells occurred at the termination of the brown tide bloom in New Jersey in 2002. Intracellular VLPs were present throughout the bloom event. The percentage of visibly infected cells was higher at the beginning of the bloom than during the peak of the bloom. The intracellular VLPs in natural populations ofA. anophagefferens were consistent in size and shape (approximately 140 nm in diameter) and comparable to those in previous studies. Concentrated viral isolates, prepared from waters during brown tide blooms in New York and New Jersey in 2002, infected healthy laboratoryA. anophagefferens cultures in vitro. The viral isolates associated with the highest laboratory viral activity (lysis positive) were concentrated from water samples having the highest viral and bacteria concentrations. The intracellular viruses in these virally infected laboratory cultures ofA. anophagefferens were similar in size and shape to those found in natural populations. The successful isolation of a virus specific toA. anophagefferens from a brown tide bloom in the field, the similarity of ultrastructure of VLPs infecting both natural populations and laboratory infected cultures, and the pattern of VLP infection during bloom activity in combination with the observed high percentage of VLP-infected cells during bloom termination, supports, the hypothesis that viruses may be a major source of mortality for brown tide blooms in regional coastal bays of New Jersey and New York.     

A Comparison of Public Lands and Farmlands for Grassland Bird Conservation

Midwestern states have invested extensively in grasslands for wildlife conservation, yet these public lands make up a minority of grassland habitat. How effective are public grasslands, relative to private lands, for conserving native songbird populations? I compare private and public lands in southern Minnesota using bird survey data from Conservation Reserve Program (CRP) fields and public lands and assessing fragmentation in a GIS. Bird abundance and diversity were greater on CRP lands. Vegetation composition, field isolation, and field size appear to explain differences in bird counts. Land cover data show that grassland habitat on public lands is scarce and widely scattered. The CRP provides more, and here better, habitat for grassland birds. Funding partly explains this disparity. Trends in farm set‐aside program rules and distribution, which can be vary greatly over time, will strongly influence the success or failure of biodiversity conservation in this region.     

Land tenure type as an underrated legal constraint on the conservation management of coastal dunes: examples from Ireland

Legal factors play an underrated but significant role as constraints on conservation management. In this paper, based on observations in both Northern Ireland and the Republic of Ireland, mode of land tenure is assessed to determine its influence on the conservation management of coastal dunes. The tenure types considered are: private ownership, corporate private ownership, tenancy, secured tenancy, leasing, common (joint) ownership, uncertain ownership, public ownership and its subset military ownership. It is suggested that multiple private ownership is least likely to favour effective conservation management. Public ownership by a statutory conservation authority and corporate private ownership by a conservation-orientated NGO are regarded as the optimum tenure types for conservation management of coastal dunes.     

Crustal and upper mantle seismic structure of the Australian Plate, South Island, New Zealand

Seismic reflection and refraction data were collected west of New Zealand's South Island parallel to the Pacific–Australian Plate boundary. The obliquely convergent plate boundary is marked at the surface by the Alpine Fault, which juxtaposes continental crust of each plate. The data are used to study the crustal and uppermost mantle structure and provide a link between other seismic transects which cross the plate boundary. Arrival times of wide-angle reflected and refracted events from 13 recording stations are used to construct a 380-km long crustal velocity model. The model shows that, beneath a 2–4-km thick sedimentary veneer, the crust consists of two layers. The upper layer velocities increase from 5.4–5.9 km/s at the top of the layer to 6.3 km/s at the base of the layer. The base of the layer is mainly about 20 km deep but deepens to 25 km at its southern end. The lower layer velocities range from 6.3 to 7.1 km/s, and are commonly around 6.5 km/s at the top of the layer and 6.7 km/s at the base. Beneath the lower layer, the model has velocities of 8.2–8.5 km/s, typical of mantle material. The Mohorovicic discontinuity (Moho) therefore lies at the base of the second layer. It is at a depth of around 30 km but shallows over the south–central third of the profile to about 26 km, possibly associated with a southwest dipping detachment fault. The high, variable sub-Moho velocities of 8.2 km/s to 8.5 km/s are inferred to result from strong upper mantle anisotropy. Multichannel seismic reflection data cover about 220 km of the southern part of the modelled section. Beneath the well-layered Oligocene to recent sedimentary section, the crustal section is broadly divided into two zones, which correspond to the two layers of the velocity model. The upper layer (down to about 7–9 s two-way travel time) has few reflections. The lower layer (down to about 11 s two-way time) contains many strong, subparallel reflections. The base of this reflective zone is the Moho. Bi-vergent dipping reflective zones within this lower crustal layer are interpreted as interwedging structures common in areas of crustal shortening. These structures and the strong northeast dipping reflections beneath the Moho towards the north end of the (MCS) line are interpreted to be caused by Paleozoic north-dipping subduction and terrane collision at the margin of Gondwana. Deeper mantle reflections with variable dip are observed on the wide-angle gathers. Travel-time modelling of these events by ray-tracing through the established velocity model indicates depths of 50–110 km for these events. They show little coherence in dip and may be caused side-swipe from the adjacent crustal root under the Southern Alps or from the upper mantle density anomalies inferred from teleseismic data under the crustal root.     

Electrodialytic remediation of CCA-treated waste wood in pilot scale

When chromated copper arsenate (CCA)-treated wood is removed from service and turns into waste, the contents of Cu, Cr and As remain high due to the strong fixation of CCA in the wood. This high content of toxic compounds presents a disposal challenge. Incineration of CCA-treated waste wood is not allowed in Denmark; instead, the wood is to be land-filled until new methods for handling the wood are available. Since the amounts of CCA-treated wood being removed from service is expected to increase in the years to come, the need for finding alternative handling methods is very relevant. In this study, the usefulness of Electrodialytic Remediation was demonstrated for handling of CCA-treated waste wood in pilot scale. The electrodialytic remediation method, which uses a low-level direct current (DC) as the cleaning agent, combines electrokinetic movement of ions in the wood matrix with the principles of electrodialysis. It has previously been shown that it is possible to remove Cu, Cr and As from CCA-treated wood using electrodialytic remediation in laboratory scale, but until now, the method had not been studied in large scale. The pilot-scale plant used in this study was designed to contain up to 2 m³ wood chips. Six remediation experiments were carried out. In these experiments, the process was up-scaled stepwise by increasing the distance between the electrodes from initially 60 cm to finally 150 cm. The remediation time was varied between 11 and 21 days, and phosphoric acid and/or oxalic acid was used to facilitate the desorption of CCA from the wood. In the most successful of the experiments carried out, the concentration of CCA in the wood was reduced by up to 82% for Cr, 88% for Cu and at least 96% for As.     

Case study on the strategy and application of enhancement solutions to improve remediation of soils contaminated with Cu, Pb and Zn by means of electrodialysis

Numerous studies have been conducted with electrochemical removal of heavy metals from spiked kaolinite. Meanwhile, when moving from kaolinite to real soils, new factors must be taken into account—factors influencing, e.g., the buffering capacity of the soil against acidification and the adsorption/desorption processes of the heavy metals. The present study gives some examples where it is necessary to use an enhancement solution to aid desorption of Cu, Zn and Pb during electrodialytic treatment. Dependent on the composition of the pollution, different choices can be made. In the case of a Cu-polluted calcareous soil, ammonia may be used as enhancement solution, due to the formation of charged complexes between ammonia and Cu. Thus, Cu is mobile at high pH when ammonia is added and Cu can be removed without dissolving the calcareous parts. Zn is also mobilized by ammonia, but to a lesser extent than Cu. In the case of Cu, Zn and Pb at the same time, alkaline ammonium citrate may be a solution. It was shown that this enhancement solution could mobilize these three pollutants, but optimization of concentration and pH of the ammonium citrate is still needed. When choosing a remediation scheme for electrochemical treatment of an actual industrially polluted soil, this scheme must be chosen on basis of characterization of soil and pollution combination.