Setting a size-exclusion limit to remove toxic dinoflagellate cysts from ships' ballast water

Dinoflagellate cysts are well-recognized biological constituents of ships' ballast tanks. They are present in ballast water, sediments and residual water in drained tanks, and in biofilms formed on interior tank surfaces. Therefore, cysts have the potential to be released during ballast discharge. The International Maritime Organization's (IMO) Ballast Water Management Convention (promulgated February 2004) stipulates a performance standard (Annex, Regulation D2) requiring discharged ballast water contain <10 viable organisms between 10 and 50 microm per ml and <10 viable organisms 50 microm per m3. The proposed size limit has potential to exclude both the smallest toxic and the largest toxic and non-toxic dinoflagellate (and other microalgal) cysts from discharged ballast water. Despite the appropriateness of size cutoffs however, ballast water containing predominantly small cysts (<50 microm) could be deemed in compliance with the performance standard, even without treatment, while ballast water having the same concentration of larger cysts (>50 microm) could require a multiple-log reduction in abundance before its permissible discharge. Also of concern, it remains uncertain whether ballast-water treatment can remove sufficient organisms, including dinoflagellate cysts, to meet the performance standard.     

Gravity for Lithosphere Architecture Determination and Analysis: the Central Eastern Mediterranean case study

The Central-Eastern Mediterranean region is known to be a complex area due to the interaction of four tectonic plates, namely Arabia, Africa, Anatolia and Eurasia, and by the presence of an ancient oceanic crust in the Herodotus and Ionian Basins. The analysis of the available literature highlights that the distribution of the freely available geophysical data (i.e. seismic, gravity and magnetic observations) is quite disparate. In this framework, high-resolution global gravity field models, such as XGM2019e, mainly based (offshore) on satellite data, can be profitably used as a uniform dataset to study, in a coherent way, large regions. In the current work, we exploit the XGM2019e model, together with a set of a priori information, derived mainly from geophysical data retrieved from the literature, to study the structure of the crust in the Central-Eastern Mediterranean area. The study is organized in three different phases: in the first one, we enhanced the map of geological crustal provinces by means of an automatic Bayesian classification algorithm applied to the second radial derivatives of the gravitational potential. In the second phase, using as observation a grid of gravity anomalies, we applied a full three-dimensional inversion procedure (always based on a Bayesian paradigm) to estimate the mass density variations and the geometries of the main geological units in the whole study area. Finally, in the third phase, we performed a refined three-dimensional local inversion on the Cyprus area to improve the modelling of the shallowest layers. The main results of this study, carried out in the framework of the European Space Agency GIADA project, are freely available, upon request, at https://www.g-red.eu/geophysics/ .     

Impact of vegetation variability on potential predictability and skill of EC-Earth simulations

Climate models often use a simplified and static representation of vegetation characteristics to determine fluxes of energy, momentum and water vapour between surface and lower atmosphere. In order to analyse the impact of short term variability in vegetation phenology, we use remotely-sensed leaf area index and albedo products to examine the role of vegetation in the coupled land–atmosphere system. Perfect model experiments are carried out to determine the impact of realistic temporal variability of vegetation on potential predictability of evaporation and temperature, as well as model skill of EC-Earth simulations. The length of the simulation period is hereby limited by the availability of satellite products to 2000–2010. While a realistic representation of vegetation positively influences the simulation of evaporation and its potential predictability, a positive impact on 2?m temperature is of smaller magnitude, regionally confined and more pronounced in climatically extreme years.     

Improving the computation of the gravitational terrain effect close to ground stations in the GTE software

The precise computation of the vertical gravitational attraction of the topographic masses (terrain correction) is still being studied both for geodetic and geophysical applications. In fact, it is essential in high precision geoid estimation by means of the well-known remove-compute-restore technique, which is used to isolate the gravitational effects of anomalous masses in exploration geophysics. The terrain correction can be evaluated exploiting a Digital Terrain Model (DTM) in different ways, such as classical numerical integration, prisms, tesseroids, polyhedrons, and/or Fast Fourier Transform techniques. The increasing resolution of recently developed DTMs, the increasing number of observation points, and the increasing accuracy of gravity data represent, nowadays, major challenges for the terrain correction computation. Classical point mass approximation and prism based-algorithms are indeed too slow, while Fourier-based algorithms are usually too much approximate when compared to the required accuracy. In this work, we improve the Gravity Terrain Effects (GTE) algorithm, the innovative tool that exploits a combined prism-Fast Fourier Transform approach especially developed for airborne gravimetry, to compute the terrain correction on the surface of the DTM (i.e. corresponding to the ground stations and/or its vicinity). This required development of a proper adjustment of the algorithms implemented within the GTE software and also to define and implement a procedure to overcome the problems of the computation of the gravitational effects due to the actual slope of the terrain close to the stations. The latter problem is thoroughly discussed and solved by testing different solutions like concentric cylindrical rings, triangulated polyhedrons, or ultra-high resolution squared prisms. Finally, numerical tests to prove the temporal efficiency and the computational performances of the improved GTE software to compute terrain correction for ground stations are also presented.     

Ocean urea fertilization for carbon credits poses high ecological risks

The proposed plan for enrichment of the Sulu Sea, Philippines, a region of rich marine biodiversity, with thousands of tonnes of urea in order to stimulate algal blooms and sequester carbon is flawed for multiple reasons. Urea is preferentially used as a nitrogen source by some cyanobacteria and dinoflagellates, many of which are neutrally or positively buoyant. Biological pumps to the deep sea are classically leaky, and the inefficient burial of new biomass makes the estimation of a net loss of carbon from the atmosphere questionable at best. The potential for growth of toxic dinoflagellates is also high, as many grow well on urea and some even increase their toxicity when grown on urea. Many toxic dinoflagellates form cysts which can settle to the sediment and germinate in subsequent years, forming new blooms even without further fertilization. If large-scale blooms do occur, it is likely that they will contribute to hypoxia in the bottom waters upon decomposition. Lastly, urea production requires fossil fuel usage, further limiting the potential for net carbon sequestration. The environmental and economic impacts are potentially great and need to be rigorously assessed.     

A standardized method for sampling and extraction methods for quantifying microplastics in beach sand

Microplastics are ubiquitous in the environment, are frequently ingested by organisms, and may potentially cause harm. A range of studies have found significant levels of microplastics in beach sand. However, there is a considerable amount of methodological variability among these studies. Methodological variation currently limits comparisons as there is no standard procedure for sampling or extraction of microplastics. We identify key sampling and extraction procedures across the literature through a detailed review. We find that sampling depth, sampling location, number of repeat extractions, and settling times are the critical parameters of variation. Next, using a case-study we determine whether and to what extent these differences impact study outcomes. By investigating the common practices identified in the literature with the case-study, we provide a standard operating procedure for sampling and extracting microplastics from beach sand.     

Long-term perspective on the relationship between phytoplankton and nutrient concentrations in a southeastern Australian estuary

Sixteen years (1997–2013) of physicochemical, nutrient and phytoplankton biomass (Chlorophyll-a (Chl-a)) data and a decade (2003-2013) of phytoplankton composition and abundance data were analyzed to assess how the algal community in a temperate southeastern Australian estuary has responded to decreased chronic point source nitrogen loading following effluent treatment upgrade works in 2003. Nitrogen concentrations were significantly lower (P < 0.05) following enhanced effluent treatment and Chl-a levels decreased (P < 0.05) during the warmer months. Temperature and nutrient concentrations significantly influenced temporal changes of Chl-a (explaining 55% of variability), while salinity, temperature, pH and nutrient concentrations influenced phytoplankton abundance and composition (25% explained). Harmful Algal Bloom (HAB) dynamics differed between sites likely influenced by physical attributes of the estuary. This study demonstrates that enhanced effluent treatment can significantly decrease chronic point source nitrogen loading and that Chl-a concentrations can be lowered during the warmer months when the risk of blooms and HABs is greatest.     

Aptian (Lower Cretaceous) biostratigraphy and cephalopods from north central Tunisia

Thick Aptian deposits in north central Tunisia comprise hemipelagic lower Aptian, reflecting the sea-level rise of OAE 1a, and an upper Aptian shallow marine environment characterized by the establishment of a carbonate platform facies. Carbon stable isotope data permit recognition of the OAE 1a event in the Djebel Serdj section. Cephalopods are rare throughout these successions, but occurrences are sufficient to date the facies changes and the position of the OAE1a event. Ammonite genera include lower Aptian Deshayesites, Dufrenoyia, Pseudohaploceras, Toxoceratoides and ?Ancyloceras; and upper Aptian Zuercherella, Riedelites and Parahoplites. Correlation of carbon isotope data with those of other Tethyan sections is undertaken together with the integration of planktonic foraminiferal data.     

Freshwater chlorophycean algae in recent marine sediments of the Beaufort, Laptev and Kara Seas (Arctic Ocean) as indicators of river runoff

Freshwater chlorophycean algae are characteristic organic-walled microfossils in recent coastal and shelf sediments from the Beaufort, Laptev and Kara seas (Arctic Ocean). The persistent occurrence of the chlorophycean algae Pediastrum spp. and Botryococcus cf. braunii in marine palynomorph assemblages is related to the discharge of freshwater and suspended matter from the large Siberian and North American rivers into the Arctic shelf seas. The distribution patterns of these algae in the marine environments reflect the predominant deposition of riverine sediments and organic matter along the salinity gradient from the outer estuaries and prodeltas to the shelf break. Sedimentary processes overprint the primary distribution of these algae. Resuspension of sediments by waves and bottom currents may transport sediments in the bottom nepheloid layer along the submarine channels to the shelf break. Bottom sediments and microfossils may be incorporated into sea ice during freeze-up in autumn and winter leading to an export from the shelves into the deep sea. The presence of these freshwater algae in sea-ice and bottom sediments in the central Arctic Ocean confirm that transport in sea ice is an important process which leads to a redistribution of shallow water microfossils.