Degradation of native wheat straw lignin by Streptomyces viridosporus T7A

Lignin is one of the major contributing factors toward the recalcitrance of lignocellulosic biomass. Understanding the process of lignin degradation in natural biological processes will provide useful information to develop novel biomass conversion technologies. Functional group changes in the lignin entities during the process may contribute to the cellulose degradation (utilization) by the microorganisms. In this study, compositional and advanced Fourier transform infrared, pyrolysis gas chromatography/mass spectrometry and ¹³C cross polarization/magic angle spinning nuclear magnetic resonance analysis were performed to explore the mechanism of biodegradation of wheat straw by Streptomyces viridosporus T7A. The results indicated that S. viridosporus T7A removed lignin and hemicelluloses as indicated by the increased carbohydrate/lignin ratio. Significant modification of carbonyl and methoxyl groups in the complex lignin structure was also evident. Most importantly, the quantitative results showed that lignin degradation was featured by deduction of guaiacyl unit. The results provide new insight for understanding lignin degradation by bacteria.     

MoisturEC: A New R Program for Moisture Content Estimation from Electrical Conductivity Data

Noninvasive geophysical estimation of soil moisture has potential to improve understanding of flow in the unsaturated zone for problems involving agricultural management, aquifer recharge, and optimization of landfill design and operations. In principle, several geophysical techniques (e.g., electrical resistivity, electromagnetic induction, and nuclear magnetic resonance) offer insight into soil moisture, but data‐analysis tools are needed to “translate” geophysical results into estimates of soil moisture, consistent with (1) the uncertainty of this translation and (2) direct measurements of moisture. Although geostatistical frameworks exist for this purpose, straightforward and user‐friendly tools are required to fully capitalize on the potential of geophysical information for soil‐moisture estimation. Here, we present MoisturEC, a simple R program with a graphical user interface to convert measurements or images of electrical conductivity (EC) to soil moisture. Input includes EC values, point moisture estimates, and definition of either Archie parameters (based on experimental or literature values) or empirical data of moisture vs. EC. The program produces two‐ and three‐dimensional images of moisture based on available EC and direct measurements of moisture, interpolating between measurement locations using a Tikhonov regularization approach.     

Diurnal Variation of Gravity Wave Activity at Midlatitudes in the Ionospheric F Region

We investigate the short-term fluctuations in the period range from 15 to 180 minutes in the electron density variations of the F region ionosphere. Electron density profiles obtained at the ionospheric stations of Pruhonice (49.9° N, 14.5° E) and Ebro (40.8° N, 0.5° E) at five minute time sampling have been used for this analysis. The diurnal changes of the activity of the acoustic gravity wave fluctuations (AGW) show a clear enhancement during and several hours after sunrise. The periods of such AGW's are about 60 to 75 minutes and these waves propagates vertically through the ionosphere from a source located at an altitude of 180-220 km. The most likely source for these events seems to be passage of the Solar terminator.     

Comparison of the LEW88516 and ALHA77005 martian meteorites: Similar but distinct

Abstract By mineral and bulk compositions, the Lewis Cliff (LEW) 88516 meteorite is quite similar to the ALHA77005 martian meteorite. These two meteorites are not paired because their mineral compositions are distinct, they were found 500 km apart in ice fields with different sources for meteorites, and their terrestrial residence ages are different. Minerals in LEW88516 include: olivine, pyroxenes (low- and high-Ca), and maskelynite (after plagioclase); and the minor minerals chromite, whitlockite, ilmenite, and pyrrhotite. Mineral grains in LEW88516 range up to a few mm. Texturally, the meteorite is complex, with regions of olivine and chromite poikilitically enclosed in pyroxene, regions of interstitial basaltic texture, and glass-rich (shock) veinlets. Olivine compositions range from Fo₆₄ to Fo₇₀, (avg. Fo₆₇), more ferroan and with more variation than in ALHA77005 (Fo₆₉ to Fo₇₃). Pyroxene compositions fall between En₇₇Wo₄ and En₆₅Wo₁₅ and in clusters near En₆₃Wo₉ and En₅₃Wo₃₃, on average more magnesian and with more variation than in ALHA77005. Shock features in LEW88516 range from weak deformation through complete melting. Bulk chemical analyses by modal recombination of electron microprobe analyses, instrumental neutron activation, and radiochemical neutron activation confirm that LEW88516 is more closely related to ALHA77005 than to other known martian meteorites. Key element abundance ratios are typical of martian meteorites, as is its non-chondritic rare earth pattern. Differences between the chemical compositions of LEW88516 and ALHA77005 are consistent with slight differences in the proportions of their constituent minerals and not from fundamental petrogenetic differences. Noble gas abundances in LEW88516, like those in ALHA77005, show modest excesses of ⁴⁰Ar and ¹²⁹Xe from trapped (shock-implanted) gas. As with other ALHA77005 and the shergottite martian meteorites (except EETA79001), noble gas isotope abundances in LEW88516 are consistent with exposure to cosmic rays for 2.5–3 Ma. The absence of substantial effects of shielding from cosmic rays suggest LEW88516 spent this time as an object no larger than a few cm in diameter.     

A Reflection on Mg, Cd, Ca, Li and Si Isotopic Measurements and Related Reference Materials

This contribution aims to report the reflections we had with the scientific community during two international workshops on reference materials for stable isotopes in Davos (2002) and Nice (2003). After evaluating the isotopic homogeneity of some existing reference materials, based on either certificates, literature data or specific inter-laboratory rounds, we confirm these as primary reference materials or propose new ones relative to which stable isotope compositions should be reported. We propose DSM-3 for Mg, NIST SRM 915a for Ca, L-SVEC for Li and NBS28 for Si. Cadmium does not yet have a well identified delta zero material, although three commercial mono-elemental Cd solutions have yielded the same isotopic composition relative to one another. In order to scale the linearity of any mass spectrometer, some secondary reference materials are also proposed: Cambridge-1 solution for Mg, the "Münster-Cd" and JEPPIM Cd solutions for Cd and the "Big Batch" silicate for Si. The team from Nancy propose to prepare a mixed spike solution for Li isotopes. Well-characterised natural samples such as ocean or continental waters, diatoms, sponges, rocks and minerals are needed to validate the entire analytical procedure, particularly to take into account the effect of sample mineralisation and of chemical manipulations for elemental separation prior to analysis.     

Thermal alteration of hydrated minerals during hypervelocity capture to silica aerogel at the flyby speed of Stardust

Abstract— Outside the Earth's atmosphere, silica aerogel is one of the best materials to capture finegrained extraterrestrial particles in impacts at hypervelocities. Because silica aerogel is a superior insulator, captured grains are inevitably influenced by frictional heat. Therefore, we performed laboratory simulations of hypervelocity capture by using light‐gas guns to impact into aerogels finegrained powders of serpentine, cronstedtite, and Murchison CM2 meteorite. The samples were shot at >6 km s^?1 similar to the flyby speed at comet P/Wild‐2 in the Stardust mission. We investigated mineralogical changes of each captured particle by using synchrotron radiation X‐ray diffraction (SR‐XRD), transmission electron microscope (TEM), and field emission scanning electron microscope (FE‐SEM). SR‐XRD of each grain showed that the majority of the bulk grains keep their original mineralogy. In particular, SR‐XRD and TEM investigations clearly exemplified the presence of tochilinite whose decomposition temperature is about 300 °C in the interior of the captured Murchison powder. However, TEM study of these grains also revealed that all the samples experienced melting and vesiculation on the surface. The cronstedtite and the Murchison meteorite powder show remarkable fracturing, disaggregation, melting, and vesiculation. Steep thermal gradients, about 2500 °C/μm were estimated near the surface of the grains (<2 μm thick) by TEM observation. Our data suggests that the interior of >4 μm across residual grains containing abundant materials that inhibit temperature rise would have not experienced >300 °C at the center.     

Late Quaternary paleoclimate of western Alaska inferred from fossil chironomids and its relation to vegetation histories

Fossil Chironomidae assemblages (with a few Chaoboridae and Ceratopogonidae) from Zagoskin and Burial Lakes in western Alaska provide quantitative reconstructions of mean July air temperatures for periods of the late-middle Wisconsin (～39,000–34,000 cal yr B.P.) to the present. Inferred temperatures are compared with previously analyzed pollen data from each site summarized here by indirect ordination. Paleotemperature trends reveal substantial differences in the timing of climatic warming following the late Wisconsin at each site, although chronological uncertainty exists. Zagoskin Lake shows early warming beginning at about 21,000 cal yr B.P., whereas warming at Burial Lake begins ～4000 years later. Summer climates during the last glacial maximum (LGM) were on average ～3.5 °C below the modern temperatures at each site. Major shifts in vegetation occurred from ～19,000 to 10,000 cal yr B.P. at Zagoskin Lake and from ～17,000 to 10,000 cal yr B.P. at Burial Lake. Vegetation shifts followed climatic warming, when temperatures neared modern values. Both sites provide evidence of an early postglacial thermal maximum at ～12,300 cal yr B.P. These chironomid records, combined with other insect-based climatic reconstructions from Beringia, indicate that during the LGM: (1) greater continentality likely influenced regions adjacent to the Bering Land Bridge and (2) summer climates were, at times, not dominated by severe cold.     

Investigation of a eutrophic tidal basin: part 1—factors affecting the distribution and biomass of macroalgae

The distribution and biomass of macroalgae, principally Enteromorpha spp., have been estimated in Langstone Harbour, a 19 km² tidal basin in southenrn England. Comprehensive mapping was carried out annually at the time of maximum biomass and monthly mapping of selected areas allowed seasonal changes to be studied. Aerial false-colour photography and ground-level mapping were used for assessing the area and density of cover of macroalgae, but have identified no continuous trends between 1973 and 1982. In nine years, some 48% of the intertidal mudflat has supported >75% macroalgal cover at some stage, but generally only one-third of this potential has been achieved annually. The average peak biomass was determined by a stratified sampling method and varied about a mean of 38.5 g dry weight per square metre and showed significant annual differences. The processes controlling macroalgal growth and distribution reviewed, but, in general, factors other than nutrient availability are effective in determining areas supporting high macroalgal cover in any particular year.     

The Askervein hill project: A finite control volume prediction of three-dimensional flows over the hill

A three-dimensional nonlinear numerical model, that has been extensively used previously to predict environmental water flows, was applied to predict the flow over an isolated hill, Askervein. Predictions are reported for winds approaching the hill from 210 ° and 180 ° clockwise from north, both under almost neutral atmospheric conditions.The model predictions were compared with data collected during a major field study in 1983. From the comparisons it was concluded that the model predicts the mean flow variables with good accuracy. Larger discrepancies were found for quantities related to the turbulence, pointing to deficiencies in the turbulence model, and perhaps in some of the measurements.     

PM2.5 co-benefits of climate change legislation part 2: California governor’s executive order S-3-05 applied to the transportation sector

California Governor’s Executive Order (CGEO) S-3-05 requires that California greenhouse gas (GHG) emissions be reduced to 80 % below 1990 levels by the year 2050. Meeting this target will require drastic changes in transportation technology, fuel, and behavior which will reduce criteria pollutant emissions as well as GHG emissions. The improvement to local air quality caused by the reduced criteria pollutant emissions must be calculated to fully evaluate the overall benefits and costs of CGEO S-3-05. In the present study, seven different transportation scenarios that move towards the goals of CGEO S-3-05 in the transportation sector were examined to determine how they would affect future airborne particulate matter (PM_2.5) concentrations in California: (1) hydrogen fuel cells, (2) electric vehicles, (3) high efficiency vehicles, (4) public mass transit, (5) biofuels, (6) biofuels + hybrid electric vehicles, and (7) hydrogen fuel cells + electric vehicles. The air quality implications of each scenario were evaluated using a chemical transport model applied during a wintertime stagnation episode representing future climate in California. Scenarios (6) and (7) reduced population-weighted PM_2.5 mass concentrations by ~9 % and PM_2.5 elemental carbon (EC) concentrations by ~30 % relative to base-case predictions.