The diet of deepwater sharks and the benefits of using DNA identification of prey

Stomachs from the sharks Dalatias licha, Centrophorus squamosus, Centroscymnus owstoni, Centroselachus crepidater, Proscymnodon plunketi, and Galeorhinus galeus were sampled from three research trawl surveys on Chatham Rise, east of New Zealand. Between 14 and 50 stomachs were examined for each species, of which 8–62% were empty. Prey were visually identified in 80 stomachs, and by DNA barcoding in a further 28 stomachs. The use of DNA methods allowed the identification of chunks of flesh found in the stomachs of D. licha and P. plunketi, and nearly doubled the rate of data accumulation for D. licha, C. squamosus, and C. owstoni. Between 84 and 223 stomachs were estimated to be needed to measure 90% of the extrapolated total prey richness. The prey of D. licha, C. squamosus, and P. plunketi were predominantly benthic or demersal fishes and cephalopods. The prey of C. owstoni and C. crepidater were predominantly mesopelagic fishes and squids. G. galeus foraged throughout the water column. Scavenging of discards from commercial fishing vessels was likely in C. squamosus, P. plunketi, and G. galeus. The diet of all species except C. crepidater was dominated by the commercially important benthopelagic species hoki Macruronus novaezelandiae.     

The impact of natural and anthropogenic forcings on climate and hydrology since 1550

A climate simulation of an ocean/atmosphere general circulation model driven with natural forcings alone (constant “pre-industrial” land-cover and well-mixed greenhouse gases, changing orbital, solar and volcanic forcing) has been carried out from 1492 to 2000. Another simulation driven with natural and anthropogenic forcings (changes in greenhouse gases, ozone, the direct and first indirect effect of anthropogenic sulphate aerosol and land-cover) from 1750 to 2000 has also been carried out. These simulations suggest that since 1550, in the absence of anthropogenic forcings, climate would have warmed by about 0.1 K. Simulated response is not in equilibrium with the external forcings suggesting that both climate sensitivity and the rate at which the ocean takes up heat determine the magnitude of the response to forcings since 1550. In the simulation with natural forcings climate sensitivity is similar to other simulations of HadCM3 driven with CO₂ alone. Climate sensitivity increases when anthropogenic forcings are included. The natural forcing used in our experiment increases decadal–centennial time-scale and large spatial scale climate variability, relative to internal variability, as diagnosed from a control simulation. Mean conditions in the natural simulation are cooler than in our control simulation reflecting the reduction in forcing. However, over certain regions there is significant warming, relative to control, due to an increase in forest cover. Comparing the simulation driven by anthropogenic and natural forcings with the natural-only simulation suggests that anthropogenic forcings have had a significant impact on, particularly tropical, climate since the early nineteenth century. Thus the entire instrumental temperature record may be “contaminated” by anthropogenic influences. Both the hydrological cycle and cryosphere are also affected by anthropogenic forcings. Changes in tree-cover appear to be responsible for some of the local and hydrological changes as well as an increase in northern hemisphere spring snow cover.

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Enteroviruses in mussels and marine sediments and depuration of naturally accumulated viruses by green lipped mussels (Perna canaliculus)

Surveys were carried out over 16 months to assess the distribution of enteroviruses of human origin in sediments and mussels near two sewage outfalls on the North Taranaki Coast, New Zealand. Enteroviruses were present in high numbers in both sediments and shellfish near the New Plymouth sewage outfall with maximum virus levels of 32 000 pfu 100 g^‐1 of wet mussel tissue and 59 pfu 100 g^‐1 of wet sediment material. Viruses were recovered occasionally from sediments and mussels near the Waitara Borough outfall. Coxsackievirus B4 was the predominant virus type isolated but CB5 and Poliovirus types 1, 2, and 3 were also recovered. Attempts to depurate virus‐contaminated New Plymouth mussels, by keeping them in water for 8 days with daily water replacement, did not achieve a significant reduction in virus numbers.     

Quantifying the Spatial Variability of Surface Fluxes Using Data from the 2002 International H2O Project

Spatial variability in the exchange of energy and moisture is a key control on numerous atmospheric, hydrologic, and environmental processes. Using observations made on fair weather days during the 2002 International H2O Project, four methods for quantifying the spatial variability of surface fluxes are investigated. The first two methods utilize applied statistical techniques to describe the spatial variability of the surface fluxes, while the third method is a geostatistical technique rooted in variography. Typically, the methods yield similar results, with median values of horizontal variability consistent to within 5%. The geostatistical technique, however, provides much more information than the other statistical methods; it not only provides an estimate of the spatial variability, but also provides estimates of the total variability, the non-spatial variability due to measurement error, and the range of spatial correlation among the data points. The fourth method is based on the relationship between the components of the surface energy budget. This method describes the variability in the fluxes in terms of the slope of the best-fit line relating the time-averaged latent and sensible heat fluxes from different locations along the flight path. The meaning of the slopes can also be interpreted in terms of the spatial variability in the available energy. For four of the five days analyzed, the key control on the spatial variability in the turbulent heat fluxes was horizontal variability in the soil heat flux. In turn, the soil heat flux varied as a function of surface properties including surface temperature, soil moisture content, and leaf area index. On the remaining day, 25 May, the primary control was the variability in net radiation.     

The Hα/Hβ ratio in solar flares

We have measured the ratio of H to H central intensities in the peak kernels of 14 flares, using simultaneous filtergrams. The ratio is typically one with some scatter. By contrast, in stellar flares the ratio is about 0.8.     

Increased toxicity of benzo(a)pyrene-7,8-dihydrodiol in the presence of polychlorobiphenylols

Benzo(a)pyrene (BaP) and polychlorinated biphenyls (PCBs) often co-exist in contaminated environments. Polychlorobiphenylols (OH-PCBs), formed by CYP-dependent monooxygenation of PCBs, are potent inhibitors of the glucuronidation of hydroxylated BaP metabolites. We hypothesized that OH-PCBs could drive the biotransformation of (−)BaP-7,8-dihydrodiol (BaP-7, 8-D) away from detoxication and towards formation of the reactive metabolite. A mixture of five OH-PCBs with 4–6 Cl atoms was infused into isolated, perfused, biliary intact livers (n=3 fish) removed from 3-methylcholanthrene-induced channel catfish. Controls (n=3) were infused with vehicle. Subsequently, [³H]-BaP-7, 8-D was infused into each liver and bile was collected for 1 h. The livers were taken for analysis of metabolites and DNA adducts. Induction status was confirmed by EROD assay. Bile was analyzed for metabolites. It was found that preinfusion of the mixture of OH-PCBs reduced the extent of glucuronidation of BaP-7, 8-D and increased the formation of DNA adducts 5-fold over controls. GSH conjugates, tetrols and triols were increased in the OH-PCB-infused fish, providing further support for our hypothesis that if the glucuronidation were inhibited, CYP-dependent activation would increase. These studies suggest a mechanism for synergy of toxicity of PAH and PCBs.     

Glucuronidation in the polar bear (Ursus maritimus)

Polar bears bioaccumulate lipophilic pollutants, including polychlorinated biphenyls (PCBs), into their bodies from their exclusive diet of marine organisms. Hydroxylated PCB metabolites (OH-PCBs) have been found in plasma, presumably due to CYP-dependent biotransformation of PCBs in liver. Little is known about the phase 2 metabolism of hydroxylated xenobiotics in polar bears. The objective of this study was to examine UDP-glucuronosyltransferase (UGT) activity with OH-PCBs and a hydroxylated polycyclic aromatic hydrocarbon, 3-hydroxy-benzo(a)pyrene (3-OH-BaP), in polar bear liver. Samples of frozen polar bear liver were used to prepare microsomes. UGT activity with 3-OH-BaP in Brij-treated microsomes, measured by a fluorescence assay, was readily measurable with protein concentrations in assay tubes of up to 10 μg/ml, but dropped off very sharply at higher protein concentrations. The apparent K_m for 3-OH-BaP was 1.71 ± 0.04 μM, and V_max 1.26 ± 0.16 nmol/min/mg protein (mean ± SD, n=3). UGT activities with a model tetrachloro-OH-PCB (4^′-OH-CB72) and a model hexachloro-OH-PCB (4^′-OH-CB159) were assayed with [¹⁴-C]-UDPGA and separation of the [¹⁴-C]-glucuronide by ion-pair extraction and thin-layer chromatography. [¹⁴-C]-glucuronide conjugates were readily formed by polar bear liver microsomes in the absence of added substrate, apparently from contaminants present in liver. This phenomenon was not observed using hepatic microsomes from laboratory-held catfish. Glucuronidation efficiency was much higher with 4^′-OH-CB72 (K_m 7.3 μM; V_max 1.55 nmol/min/mg) than 4^′-OH-CB159 (K_m 16.1 μM; V_max 0.46 nmol/min/mg). The identities of the aglycones present in polar bear liver are not known, but could include OH-PCBs or hydroxylated metabolites of other persistent organic pollutants. This study demonstrates that UGT with high activity for 3-OH-BaP and other substrates is present in polar bear liver.     

Catalytic properties of cytochrome P-450 in hepatopancreas of the spiny lobster, Panulirus argus

These studies were designed to determine the metabolic capability of the microsomal cytochrome(s) P-450 in spiny lobster hepatopancreas, and to determine how chemicals which selectively modify mammalian monooxygenase activity catalyzed by different cytochrome P-450 isozymes affect the spiny lobster cytochrome P-450. We used a washing procedure to concentrate the hepatopancreas microsomal cytochrome P-450 and remove the inhibitors of monooxygenase activity which are normally present in microsomes. The resulting reparation (MI fraction) was used to determine monooxygenase activity towards benzo[a]pyrene, benz-phetamine, 7-ethoxyresorufin in the presence of either cumene hydroperoxide or NADPH and vertebrate liver cytochrome P-450 reductase. Benzphetamine was the best substrate for the lobster cytochrome P-450, whereas 7-ethoxyresorufin was metabolized very slowly. Studies with chemical modifiers showed that the responses of the lobster cytochrome(s) P-450 were not similar to those of any of the well-characterized cytochrome P-450 isozymes purified from mammalian liver.