Increased toxicity of benzo(a)pyrene-7,8-dihydrodiol in the presence of polychlorobiphenylols

Benzo(a)pyrene (BaP) and polychlorinated biphenyls (PCBs) often co-exist in contaminated environments. Polychlorobiphenylols (OH-PCBs), formed by CYP-dependent monooxygenation of PCBs, are potent inhibitors of the glucuronidation of hydroxylated BaP metabolites. We hypothesized that OH-PCBs could drive the biotransformation of (-)BaP-7,8-dihydrodiol (BaP-7, 8-D) away from detoxication and towards formation of the reactive metabolite. A mixture of five OH-PCBs with 4-6 Cl atoms was infused into isolated, perfused, biliary intact livers (n=3 fish) removed from 3-methylcholanthrene-induced channel catfish. Controls (n=3) were infused with vehicle. Subsequently, [3H]-BaP-7, 8-D was infused into each liver and bile was collected for 1 h. The livers were taken for analysis of metabolites and DNA adducts. Induction status was confirmed by EROD assay. Bile was analyzed for metabolites. It was found that preinfusion of the mixture of OH-PCBs reduced the extent of glucuronidation of BaP-7, 8-D and increased the formation of DNA adducts 5-fold over controls. GSH conjugates, tetrols and triols were increased in the OH-PCB-infused fish, providing further support for our hypothesis that if the glucuronidation were inhibited, CYP-dependent activation would increase. These studies suggest a mechanism for synergy of toxicity of PAH and PCBs.     

Effect of in vivo cadmium exposure on the respiratory burst of Marine Fish (Limanda limanda L.) phagocytes

Following the in vivo exposure of dab (Limanda limanda L.) to cadmium chloride, kidney phagocytes were collected and their respiratory burst measured in vitro using chemiluminescence. Fish were exposed to mean measured concentrations of 1.3, 2.7 and 5.5 mg Cd litre⁻¹ (as total cadmium ion) for a total of nine weeks, followed by a three week depuration period in clean sea water. Compared with control fish, the respiratory burst of kidney phagocytes from dab sampled after six weeks was significantly reduced in the 2.7 and 5.5 mg Cd litre⁻¹ treatments (Steel's test, p < 0.05). Significant reductions were observed in the respiratory burst of phagocytes from all cadmium exposed fish compared with control fish after nine weeks (Steel's test, p < 0.05). After a further three week depuration period in clean sea water, the respiratory burst of phagocytes from fish previously exposed to 1.3 and 2.7 mg Cd litre⁻¹ were still significantly less than in the control group (Steel's test, p < 0.05). Muscle tissue cadmium concentrations were also analysed, although there was no clear relationship between the muscle total cadmium levels and kidney phagocyte chemiluminescence. The results are discussed with respect to the possible mechanism(s) of cadmium immunotoxicity in dab and recommendations made for future work.     

Identification of transcriptional effects of ethynyl oestradiol in male plaice (Pleuronectes platessa) by suppression subtractive hybridisation and a nylon macroarray

Suppression subtractive hybridisation (SSH) was used to generate cDNA libraries representing genes differentially expressed in response to ethynyl oestradiol (EE2) exposure in liver from male plaice (Pleuronectes platessa) previously analysed for vitellogenin (VTG) induction. Characterisation of the cDNA clones identified many as VTG (2 genes) and zona radiata proteins (ZRP) (3 genes), but 40 encoded other proteins, with more than half cryptic. Further analysis identified 85 non-redundant clones suitable for array on nylon membrane. Radiolabelled cDNAs were prepared from hepatic mRNA from EE2 treated plaice (0 and 21 days) and hybridised with the arrayed clones. Analysis of the data showed that 11/17 novel, 21/22 VTG, 13/14 ZRP, 2/2 liver aspartic proteinase (LAP) and 8/10 other mRNAs were up-regulated by EE2 exposure.     

Neutrino-pair bremsstrahlung in collisions between neutrons and nuclei in neutron star matter

In neutron star matter over the density range 4.3×10¹¹<-ϱ≤4×10¹⁴ g cm⁻³ there are both free neutrons and neutron-rich nuclei. If there is a weak neutral current interaction between neutrinos and neutrons, as suggested by recent experiments, then when neutrons scatter off nuclei they may emit pairs as bremsstrahlung radiation. We calculate the associated emissivity for degenerate (but not superfluid) neutrons and uncorrelated (not crystallized) nuclei. We find that, under these conditions. this emissivity can under some conditions compare with that calculated by Festa and Ruderman for bremsstrahlung in electron-nucleus collisions.     

Petrology and geochemistry of the 2014–2015 Holuhraun eruption,central Iceland: compositional and mineralogical characteristics,temporal variability and magma storage

The 2014–2015 Holuhraun fissure eruption provided a rare opportunity to study in detail the magmatic processes and magma plumbing system dynamics during a 6-month-long, moderate- to large-volume basaltic fissure eruption. In this contribution, we present a comprehensive dataset, including major and trace elements of whole-rock and glassy tephra samples, mineral chemistry, and radiogenic and oxygen isotope analyses from an extensive set of samples (n?=?62) that were collected systematically in several field campaigns throughout the entire eruptive period. We also present the first detailed chemical and isotopic characterization of magmatic sulfides from Iceland. In conjunction with a unique set of geophysical data, our approach provides a detailed temporal and spatial resolution of magmatic processes before and during this eruption. The 2014–2015 Holuhraun magma is compositionally indistinguishable from recent basalts erupted from the Bárðarbunga volcanic system, consistent with seismic observations for magma ascent close to the Bárðarbunga central volcano, followed by dyke propagation to the Holuhraun eruption site. Whole-rock elemental and isotopic compositions are remarkably constant throughout the eruption. Moreover, the inferred depth of the magma reservoir tapped during the eruption is consistently 8?±?5 km, in agreement with geodetic observations and melt inclusion entrapment pressures, but inconsistent with vertically extensive multi-tiered magma storage prior to eruption. The near constancy in the chemical and isotopic composition of the lava is consistent with the efficient homogenization of mantle-derived compositional variability. In contrast, occurrence of different mineral populations, including sulfide globules, which display significant compositional variability, requires a more complex earlier magmatic history. This may include sampling of heterogeneous mantle melts that mixed, crystallized and finally homogenized at mid- to lower-crustal conditions.     

An Observational Study of Wind Profiles in the Baroclinic Convective Mixed Layer

A comprehensive planetary boundary-layer (PBL) and synoptic data set is used to isolate the mechanisms that determine the vertical shear of the horizontal wind in the convective mixed layer. To do this, we compare a fair-weather convective PBL with no vertical shear through the mixed layer (10 March 1992), with a day with substantial vertical shear in the north-south wind component (27 February). The approach involves evaluating the terms of the budget equations for the two components of the vertical shear of the horizontal wind; namely: the time-rate-of-change or time-tendency term, differential advection, the Coriolis terms (a thermal wind term and a shear term), and the second derivative of the vertical transport of horizontal momentum with respect to height (turbulent-transport term). The data, gathered during the 1992 STorm-scale Operational and Research Meteorology (STORM) Fronts Experiments Systems Test (FEST) field experiment, are from gust-probe aircraft horizontal legs and soundings, 915-MHz wind profilers, a 5-cm Doppler radar, radiosondes, and surface Portable Automated Mesonet (PAM) stations in a roughly 50 × 50 km boundary-layer array in north-eastern Kansas, nested in a mesoscale-to-synoptic array of radiosondes and surface data.We present evidence that the shear on 27 February is related to the rapid growth of the convective boundary layer. Computing the shear budget over a fixed depth (the final depth of the mixed layer), we find that the time-tendency term dominates, reflecting entrainment of high-shear air from above the boundary layer. We suggest that shear within the mixed layer occurs when the time-tendency term is sufficiently large that the shear-reduction terms – namely the turbulent-transport term and differential advection terms – cannot compensate. In contrast, the tendency term is small for the slowly-growing PBL of 10 March, resulting in a balance between the Coriolis terms and the turbulent-transport term. Thus, the thermal wind appears to influence mixed-layer shear only indirectly, through its role in determining the entrained shear.     

Water quality, nutrients and the water framework directive in an agricultural region: The lower Humber Rivers, northern England

The water quality of rivers in the eastern part of the Humber Basin, north-eastern England is described from a baseflow survey (11–13 August 2006) of a wide range of water quality determinants, and long-term nutrient records of the Environment Agency of England and Wales (EA). The baseflow survey shows that the rivers are oversaturated with respect to dissolved CO₂ and calcite. They are sodium, potassium, lithium, boron, chloride, sulphate and fluoride bearing from a combination of atmospheric, within-catchment, agricultural and sewage effluent sources. Nitrate concentrations are uniform for rivers draining permeable bedrock but decrease for clay drainage areas. Soluble reactive phosphorus (SRP) concentrations are variable across the catchments reflecting the importance and variability of point sources and within-river processing. The EA data show annual oscillations for both NO₃ and SRP concentrations. Average NO₃ concentrations vary between 3.3 and 18.8 mgN/l and concentrations are low during the summer months. Average SRP concentrations vary between 23 and 1959 μg/l. Highest SRP concentrations generally occur when there is effluent input from sewage treatment works and agricultural point sources (e.g. overflow from slurry tanks, farmyard washings). Despite many of the rivers being nutrient rich, they are generally of good biological quality when point source inputs are not important.     

Organochlorine contaminants in endangered Hawaiian monk seals from four subpopulations in the Northwestern Hawaiian Islands

We analyzed blubber and blood samples for organochlorines (OCs) from 158 Hawaiian monk seals (Monachus schauinslandi) at four of their six primary breeding colonies in the Northwestern Hawaiian Islands. Levels of OCs in blubber were lower in adult females compared to juveniles or adult males, evidently owing to the transfer of these lipophilic compounds to pups by pregnant and lactating females. Concentrations of SigmaPCBs and p,p'-DDE in blubber generally increased with age until seals were sexually mature and then continued to increase with age in males after puberty. Average levels of PCBs and PCB toxic equivalents (TEQs) in blubber were significantly higher in adult male and juvenile seals at Midway Atoll than the same age class of seals at the other colonies. Unlike concentrations of OCs in blubber, circulating levels of OCs in blood did not vary consistently among age classes of seals or among breeding colonies. Though the concentrations of OCs measured in Hawaiian monk seals were generally equal to or lower than those reported for other pinniped species in the North Pacific Ocean, they were high enough in a few seals to potentially affect their health.     

On board short-time high temperature heat treatment of ballast water: a field trial under operational conditions

A ballast water short-time high temperature heat treatment technique was applied on board a car-carrier during a voyage from Egypt to Belgium. Ballast water from three tanks was subjected for a few seconds to temperatures ranging from 55 degrees C to 80 degrees C. The water was heated using the vessel's heat exchanger steam and a second heat exchanger was used to pre-heat and cool down the water. The treatment was effective at causing mortality of bacteria, phytoplankton and zooplankton. The International Maritime Organization (IMO) standard was not agreed before this study was carried out, but comparing our results gives a broad indication that the IMO standard would have been met in some of the tests for the zooplankton, in all the tests for the phytoplankton; and probably on most occasions for the bacteria. Passing the water through the pump increased the kill rate but increasing the temperature above 55 degrees C did not improve the heat treatment's efficacy.