Induction of heat shock protein (hsp)60 in Isochrysis galbana exposed to sublethal preparations of dispersant and Prudhoe Bay crude oil

Adaptation to sublethal exposure to crude oil by phytoplankton is poorly understood. Use of chemical dispersants for oil spill remediation increases petroleum hydrocarbon concentrations in water, while exposing marine organisms to potentially toxic concentrations of dispersant. Heat shock proteins (hsps) have been found to serve as an adaptive and protective mechanism against environmental stresses. The objective of this project was to examine the induction of hsps in Isochrysis galbana, a golden-brown algae, following exposure to the water-accommodated fraction (WAF) of Prudhoe Bay crude oil (PBCO) and PBCO chemically dispersed with Corexit 9527® (dispersed oil: DO). Initial experiments using ³⁵S-labeled amino acids and 2-dimensional electrophoresis with subsequent western blotting identified and confirmed hsp60, a member of the chaperonin family of stress proteins, as being efficiently induced by heat shock in this species. One-dimensional SDS PAGE and western blotting, with hsp60 antibodies and chemiluminesence detection, were used to quantitate hsp60 following exposure to a range of environmental temperatures and concentrations of WAF and DO preparations. I. galbana cultured in 22 parts per thousand (‰) salinity showed a statistically significant increase (p<0.05) in hsp60 after exposure to 25, 30, and 35°C for 1 h compared to controls (20°C), while 34‰ cultures showed no significant increase in hsp60 with increasing temperature. Cultures (22 and 34‰) exposed to varying doses of WAF and DO exhibited statistically significant (p<0.05) increases in levels of hsp60 in all cases. A dose-related response was observed in all exposures (r²≥0.90) with the exception of 34‰ WAF. In independent exposures, a dose-related hsp60 response to naphthalene, a relatively abundant water-soluble aromatic hydrocarbon, was observed in both 22 and 34‰ cultures. Results of this study are consistent with previous studies in other species documenting increases in hsp60 levels with exposure to xenobiotics. Further studies are investigating the protective function of hsp60 against the toxic effects of exposure to WAF and DO preparations.     

Evaluation of the Variability of the Shoreline of the Tsimlyansk Reservoir and Lake Ilmen according to Space Sounding Data

Izvestiya, Atmospheric and Oceanic Physics - This article presents the results of a study of the dynamics of the shorelines of Lake Ilmen and the Tsimlyansk Reservoir to indicate the location of...     

Structure of the basins of the White Sea rift systems

For the first time, the structure of the sedimentary basins of the Late Proterozoic rift system in the White Sea is characterized based on a set of new marine geological geophysical data such as the results of the common depth point seismic method, gravity and magnetic data, and seismoacoustics. The main tectonic structures in the topography of the heterogeneous basement within the basin of the White Sea are distinguished and described. A structural tectonic scheme of the basement surface is presented. The thicknesses of the sediments are estimated and the stratigraphic confinement of the seismic units recognized is done.     

Structure and Distribution of Autumn Zooplankton in the Southeastern Baltic Sea in 2015

Oceanology - Specific features of the structure and distribution of autumn zooplankton in the southeastern Baltic Sea (SEB) in October 2015 have been found taking into account the hydrological and...     

Review of Methods to Retrieve Sea-Ice Parameters from Satellite Microwave Radiometer Data

Izvestiya, Atmospheric and Oceanic Physics - Sea-ice monitoring using long-term data from satellite passive microwave instruments allows one to make quantitative estimates of climatic trends. These...     

On Methods of Short-Term Earthquake Prediction Based on Monitoring the Hydrogeodeformation Field

Izvestiya, Atmospheric and Oceanic Physics - The permanent analysis of the hydrogeodeformation (HGD) field is one promising direction in the field of predicting strong earthquakes. As a result of...     

Applied Aspects of Different Frequency Bands of Seismic and Water Acoustic Investigations on the Shelf

Izvestiya, Atmospheric and Oceanic Physics - This article focuses on the study of the information value of offshore technologies based on the generation of acoustic signals in water in a wide...     

Hydrophysical state of the Large Aral Sea in the autumn of 2013: Thermal structure,currents, and internal waves

This paper is focused on the hydrophysical state of the western basin of the Large Aral Sea in its present conditions of ecological crisis. The investigation is based on the direct observations carried out during a field survey in the Aral Sea in October of 2013. The analysis of the distribution of the temperature values across the western basin is given. Time series of the water currents variability were recorded at four mooring stations. The pattern of the basin-scale circulation response to the wind forcing was described. Based on the in situ measurements of the time variability of the temperature fields, the principal characteristics of the internal waves were specified for the present-day Aral Sea for the first time.     

Transformation of a strongly nonlinear wave in a shallow-water basin

The transformation of a nonlinear wave in shallow water is investigated analytically and numerically within the framework of long-wave theory. It is shown that the nonlinearity parameter (the Mach number), which is defined as the ratio of the particle velocity in the wave to the propagation velocity, can be well above unity in a deep trough and that a jump appears initially in the trough. It is demonstrated that shockwave amplitudes at large times change in accordance with the prediction of weakly nonlinear theory. The shock front generates a reflected wave, which, in turn, transforms into a shock wave if the initial amplitude is large enough. The amplitude of the reflected wave is proportional to the cube of the initial amplitude (as predicted by weakly nonlinear theory) over a wide range of amplitudes except for the case of anomalously strong nonlinearity. When there is a sign-variable sufficiently intense initial perturbation, the basic wave transforms into a positive shock pulse (crest) and the reflected wave turns into a negative pulse (trough).