Detection of the end point temperature of thermal denatured protein in fish and chicken meat through SDS-PAGE electrophoresis

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied in the detection of the end point temperature (EPT) of thermal denatured protein in fish and meat in this study. It was also used in studying the thermal denatured temperature range of proteins in salmon and chicken meat. The results show that the temperature ranges of denatured proteins were from 65 ℃ to 75 ℃ , and these temperature ranges were influenced by the processing methods. Through SDS-PAGE, the features of repeated he...     

Comparisons of several evaporation/precipitation datasets for the Bohai Sea based on salinity simulation

Evaporation (E) rate and precipitation (P) rate are two significant meteorological elements required in the ocean baroclinic modeling as external forcings. However, there are some uncertainties in the currently used E/P rates datasets, especially in terms of the data quality. In this study, we collected E/P rates data from ERA-40, NCEP/NCAR Reanalysis, HOAPS for the Bohai Sea and nine routine stations around Laizhou Bay, and made comparisons among them. It was found that the differences in E/P rates between land and sea are remarkable, which was due to the difference in underlying surfaces. Therefore, the traditional way of using E/P rates acquired on land directly at sea is not correct. Since no final conclusion has been reached concerning the net water transport between the Bohai Sea and the Yellow Sea, it is unfeasible to judge the adequacy of the four kinds of data by using the water budget equation. However, the E/P rates at ERA-40 sea points were considered to be the optimal in terms of temporal/spatial coverage and resolution for the hindcast of salinity variation in the Bohai Sea. Besides, using the 3-D hydrodynamic model HAMSOM (HAMburg Shelf Ocean Model), we performed numerical experiments with different E/P datasets and found that the E/P rates at sea points from ERA-40 dataset are better than those from NCEP/NCAR Reanalysis dataset. If NCEP/NCAR Reanalysis E/P rates are to be used, they need to be adjusted and tested prior to simulation so that more close-to-reality salinity values can be reproduced.     

Cloning and phylogenetic analysis of a fatty acid elongase gene from Nannochloropsis oculata CS179

Nannochloropsis oculata CS179, a unicellular marine microalga, is rich in long-chain polyunsaturated fatty acids (LCPUFAs). Elongase and desaturase play a key role in the biosynthesis of PUFAs. A new elongase gene, which encodes 322 amino acids, was identified via RT-PCR and 5′ and 3′ RACE. The sequence of the elongase gene was blast-searched in the NCBI GenBank and showed a similarity to those of the cryptosporidium. But the NJ-tree revealed that the N. oculata CS179 elongase clustered with those of the microalgae Phaeodactylum tricornutum, Ostreococcus tauri and Thalassiosira pseudonana.     

Formation of saddle dolomites in Upper Cambrian carbonates, western Tarim Basin (northwest China): Implications for fault-related fluid flow

The saddle dolomites occur more intensely in cores closely to fault than that in cores far away from the fault in Upper Cambrian carbonate of western Tarim basin, suggesting that formation of the saddle dolomites is likely related to fault-controlled fluid flow. They partially fill in fractures and vugs of replacement dolomite. The saddle dolomites exhibit complex internal textures, commonly consisting of core and cortex. In comparison with the matrix dolomites, the saddle dolomites show lower Sr-content and ⁸⁷Sr/⁸⁶Sr ratios, higher Fe- and Mn-content, and more negative δ¹⁸O values. Combined with high Th (100–130 °C) of primary fluid inclusions, it is suggested that the saddle dolomites precipitated from hydrothermal fluid derived from the deep evaporite-bearing Middle Cambrian strata, and the magnesium source may be due to dissolution of host dolomite during hydrothermal fluid migration. Fault activity resulted in petrographic and geochemical difference of the core and cortex of the saddle dolomites. The cores precipitated from the formation water mixed by deep brines at the early stage of fault activity, and the cortexes precipitated from the deep fluid with higher temperatures through the Middle Cambrian later. In summary, the formation of the saddle dolomites implies a hydrothermal fluid event related to fault activity, which also resulted in high porosity in Upper Cambrian carbonate in western Tarim Basin.     

三维含拓扑地质剖面三角剖分可视化研究

三维剖面是地质对象表达和地质问题分析中最基本和最常用的数据。该文分析三维复杂地质折剖面可视化存在的问题,并提出展开剖分算法。该方法保证了各点之间的水平距离与剖面拓扑关系不变,能够对由"多边形-弧段-结点"矢量结构构成的三维折剖面进行有效剖分。在此基础上实现了三维剖面的可视化,尤其合理解决了纹理映射中的纹理变形与重复接缝等问题。     

Comparative analysis on microbial community associated with different gastrointestinal regions of wild northern snakehead Channa argus Cantor, 1842

Microbial communities in different gastrointestinal regions(stomach, foregut, midgut, and hindgut) of the northern snakehead C hanna argus(Cantor, 1842) were compared by polymerase chain reaction and partial 16 S r DNA sequencing. A total of 194, 140, 212, and 122 OTUs were detected in the stomach, foregut, midgut, and hindgut, respectively. Significant dif ferences were found in the Sobs, ACE, Shannon, and Simpson indices among samples(P 0.05). The gastrointestinal microbial community of C. argus consisted predominantly of Proteobacteria with either H alomonas, Shewanella, Plesiomonas, or Sphingomonas. Fusobacteria, Firmicutes, and Bacteroidetes also existed in the gastrointestinal tracts. However, significant diff erences were found in the compositions of microbial community among the four regions( P 0.05). Cyanobacteria and Spirochetes were significantly higher in the midgut and hindgut( P 0.05). Fusobacteria and Firmicutes were dominant in the hindgut and foregut, respectively(P 0.05). Proteobacteria was the lowest in the hindgut( P 0.05). At genus level, C etobacterium and Plesiomonas were significantly higher in the hindgut than in the other three samples( P 0.05). Clostridium and Prevotella were the highest in the midgut(P 0.05). Halomonas, Shewanella, and S phingomonas were the highest in the foregut( P 0.05). Paracoccus and Vibrio were the highest in the stomach. Several genera were only detected in certain regions, as follows: stomach, P aracoccus and Vibrio; foregut, Halomonas, Shewanella, and Sphingomonas; midgut, Clostridium and Prevotella; and hindgut, C etobacterium and Plesiomonas( P 0.05). At the species level, A cinetobacter rhizosphaerae was only detected in the stomach. P revotella copri and Clostridium perfring were not detected in the foregut and midgut, respectively, whereas Prevotella copri and Faecalibacterium pra were not detected in the hindgut. These findings provide valuable information on the microbial community in each gastrointestinal region of C. argus. Moreover, this study indicated that microbial community was not only related to rearing environment but also to the physico-chemical characteristics of each gastrointestinal region.     

Co-dwelling,Mix-dwelling and Dis-dwelling: The Diversity Among Three Human and Livestock Dwelling Forms in Rural China

Humans and livestock have always dwelling together, especially in rural areas. Based on a multi-method approach of ethnographic fieldwork, grounded theory and one-way multivariate analysis of variance, this paper explores human and livestock dwelling. The findings show that human and livestock dwelling form a special type of human dwelling place, with forms defined as co-, mix- and dis-dwelling. Then, from co-, mix- to dis-dwelling constructed a story line to the evolution of human and livestock dwelling forms, which further explains the entire dwelling situation of humans and livestock, including the dimensions of human dweller, livestock dweller, the rural environment and special events. Among co-, mix- and dis-dwelling significant differences existed between human and livestock individuals, the nature of rural environment, vernacularity and rural tourism development. Between co- and dis-dwelling, significant differences existed in seventeen aspects; between mix- and dis-dwelling, significant differences existed in eleven aspects; meanwhile, no significant differences existed between co- and mix-dwelling. Accordingly, this suggested that human and livestock dwelling forms can be divided into mix- and dis-dwelling. This study on humans and livestock dwelling relationship offer a new perspective on rural and animal geography. This paper is an exploratory foray into rural dwelling geography as part of the Anthrozoology study and broadens the scope of extant scholarship on human dwelling morphology, as well as analyses the spatial relationships between human and livestock dwelling.     

痕量铁和锰的化学发光检测

评述了国内外有关铁、锰的化学发光分析进展。从发光体系、操作方式、最佳条件、干扰离子、检测限等各个方面对化学发光法测定铁、锰及其它们与其它组分同时测定中的应用进行分类评述。     

天姆尖盆地基本特征及其铀成矿条件分析

通过综合区调,笔者认为赣杭构造带东段天姆尖盆地是一个大型炎山塌陷盆地,其铀成矿条件良好,是陆相火山岩型富大铀矿有利的找矿靶区之一。本文详细论述了天姆尖盆地的区域地质特征、盆地的基底与盖层特征,厘定了盆地的性质,并根据区域地球物理场特征推断盆地的深部地质结构,全面分析了该盆地的铀成矿条件,指出找矿前景。