Diagnosis of iridovirus in large yellow croaker by PCR

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10^-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.     

刀额新对虾染色体核型及细胞核DNA含量

以刀额新对虾胚胎、无节幼体、卵巢、精巢等为材料 ,空气干燥法制备染色体 ,并初步进行核型分析。结果表明 ,刀额新对虾的染色体数目为 2n=78,n =39,核型为 2n=78=40M 1 0SM 1 4ST 1 4T。以刀额新对虾血淋巴、卵巢、肌肉、鳃为材料 ,以鸡血细胞为DNA标准 (2 50pg/ 2c) ,使用PartecCCAⅡ型流式细胞仪测定了刀额新对虾细胞的基因组DNA含量 ,其值为鸡血细胞的 1 75倍 ,绝对含量为 4 375pg/ 2c。     

A new type of Shipboard Meteorological Satellite Receiving-processing System

INTRODUCTIONTheSMSRPSisstalledontheshipnavigatingovertheseaandreceivessatellitecloudmapsatanytimetoprovidereliablereal-timedataofmeteorologyandocean.Itisanimportantequipmentforsafeguardofshipnavigation.Chinahasthousandsofoceanicships.Butalmostallships,withonlyveryfewexception,arenotstalledbySMSRPS.Thecausesareasfollows:1.Thetechniqueisverycomplicated.2.Thedevelopmentcostisveryhigh.3.Ifweintroduceforeignequipment,thecostistooexpensive.4.Foreignequipmentanditssoftwarearecompletelyclo…     

赤道暖池区漂流浮标运动轨迹分析

TOGA—COARE强化观测期间,对赤道暖池区海流作了多种方法、多层次的观测;根据美国释放的漂流浮标不同时刻位置的资料,分别对赤道及其南、北海域的表层漂流状况作了计算分析,指出:从1°N向北存在单一的北向流;从1°N～1°S这个近赤道区域内为东向流;1°N～2°S区域为过渡区,以东向流为主,个别浮标出现涡旋状运动。2°S以南为一反时针运动的大涡旋。     

海洋岛海域水温异常波动与养殖栉孔扇贝死亡的关系

本文对海洋岛海域水温异常波动,与该海域养殖栉孔扇贝大批死亡的关系,进行了研究。首先,总结了几年来海洋岛海域养殖栉孔扇贝死亡的特征。其次,结合水温观测数据分析了水温异常波动与栉孔扇贝大批死亡的关系。得出的结论是:大幅度、跳跃型水温异常波动是导致扇贝死亡主要原因。最后,通过生物试验证实了上述观点。     

长江口拦门沙地区的泥沙运动规律

依据１９８８年７月和１２月水文观测资料，通过流，盐，沙的综合分析，认识到长江口拦门沙地区的泥沙运动有与河口其他地区不同的特点，泥沙输移在水平面上和垂直面上存在着多种循环，潮流和盐水截留大量泥沙积聚的拦门沙地区，河口在盐淡水交锋地带，除了涨，落急时段外，发现在转流时期泥沙也可能产生再悬浮，从而形成一个潮周期中出现３次或４次再悬浮的特殊规律。     

厦门岛市区机动车流的航空遥感监测

本文介绍了厦门岛市区机动车流遥感监测的实施目的和方法，通过航空影像有关题信息解译与实例、专题图件编制以及统计资料的对比分析，认为：目前厦门岛市区交通主要存在着机动车增长过速、道路狭窄和会计室场少且分布不合理等三个方面的问题，文中提出了相应的建议。体现了航空遥感对于中、大比例尺城市动态监测快速有效的特点。     

东海大规模赤潮危害影响的实验研究——对黑褐新糠虾和卤虫的毒性效应

The effects of Prorocentrum donghaiense,Karenia mikimotoi,Alexandrium catenella,the causative species of Harmful Algal Blooms in the East China Sea,on the survival and growth of Neomysis awatschensis and Artemia salina were investigated.And the toxicity of eleven strains of Alexandrium----A.lusitanicum(AL-1)、A.minutum(AM-1)、A.spp.(A-K)、A.tamarense(ATHK,ATCI02,ATCI03,AT5-1,AT5-3,AT-6)、A.affine(AS-1,AC-1) to the two above organisms were compared. The results showed that P.donghaiense and K.mikimotoi had no adverse effect on the survival and growth of N.awatschensis and A.salina at 96h.However,the survival rate of N.awatschensis and A.salina declined linearly with the increasing density of A.catenella,and the 96h LC₅₀ for N.awatschensis and A.salina was 4500 and 460 cells/mL,respectively, Five strains of Alexandrium----A.spp,A.lusitanicum (AL-1),A.tamarense(AT5-3,AT-6) and A.affine (AS-1)at the density of 4000cells/mL and 96h had no adverse impacts on N.awatschensis. But other six strains of Alexandrium a.Tamarense(AT5-1,ATHK,ATCI02,ATCI03),A.affine(AC-1) and A.minutum(AM-1)decreased the survival fate of N.awatschensis significantly. Two strains of Alexandrium----A.lusitanicum(AL-1) and A.tamarense(AT-6),at the density of 500cells/mL. had no adverse effects on A.salina in 96h;while the other Alexandrium strains all had adverse effeets on the survival of a.salina, A.salina could increase its length with the increasing density of P.donghaiense,K.mikimotoi and A.luxitanicum. The results indicate that P.donghaiense and K.mikimotoi have nio significant effect on the survival of crustaeean zoop lankton in a short period. But several Alexandrium specised/strains could influence the survival of crustacean zooplankton likely by non-PSP substances. A.salina is shown to be more sensitive than N.awatschensis as test organisms to harmful species.     

卵形鲳鲹早期卵子发生显微及超微结构的研究

本文研究了卵形鲳鲹早期卵子发生的显微与超微结构特点。研究结果表明在1～2龄鱼卵巢中卵原细胞进入首次成熟分裂前期的结构特点，3～4龄鱼才开始进入小生长期，文中讨论了此鱼性腺成熟的年龄以及如何加速性腺发育，为人工繁殖提供科学依据。