南海沉积物细菌胞外蛋白酶在家族水平上的多样性

海洋产蛋白酶细菌及其分泌的胞外蛋白酶在降解有机氮以推动海洋氮循环进行方面发挥着重要作用,但目前对这二者多样性的认识非常有限。本研究自南海沉积物中筛选得到90株产蛋白酶细菌,并通过N-端氨基酸序列,分析了其胞外蛋白酶在家族水平上的多样性。16S rRNA基因序列分析的结果表明,筛选的产蛋白酶细菌均属于Gammaproteobacteria纲,且大多数属于Alteromonadales目和Vibrionales目的不同属。对其中14株菌株的14个胞外蛋白酶的N-端氨基酸序列分析表明,所有这些蛋白酶属于金属蛋白酶的M4家族或丝氨酸蛋白酶的S8家族。本研究提供了海洋沉积物产蛋白酶细菌在类群及其胞外蛋白酶在类型上的新细节,这将有助于全面了解微生物酶促降解海洋沉积物有机氮的过程和机理。     

仙蓉养精种玉汤治疗多囊卵巢综合征肾阴亏虚证30例临床观察

目的：观察仙蓉养精种玉汤对多囊卵巢综合征（PCOS）肾阴亏虚证患者的临床疗效。方法：选取肾阴亏虚型PCOS患者60例,采用随机数字表法将其分为治疗组与对照组,每组各30例。治疗组予以仙蓉养精种玉汤治疗,对照组予以达英-35治疗,2组均治疗3个月经周期,观察2组患者卵泡发育、排卵情况、临床疗效、中医证候积分及血清性激素水平的变化。结果：总有效率治疗组为90.00%,对照组为73.33%,2组比较,差异有统计学意义（P<0.05）;2组患者中医证候积分,排卵情况,血清T、LH、LH/FSH水平等指标治疗前后组内比较及治疗后组间比较,差异均有统计学意义（P<0.05）;治疗组治疗后对月经周期、经量、卵泡发育的改善效果均优于对照组,差异有统计学意义（P<0.05）。结论：仙蓉养精种玉汤治疗肾阴亏虚型PCOS临床疗效显著,安全性高,同时在调节性激素水平、改善患者临床症状、促进卵泡发育及排卵方面具有促进作用。     

白缘(鱼央)5S rDNA的扩增分析及其在染色体上的FISH定位

本文分别对雌雄白缘(鱼央)的5S rDNA进行了PCR扩增和序列分析,并采用双色荧光原位杂交(FISH)技术,以白缘(鱼央)的5S rDNA序列和小麦的45S rDNA为探针,对其在白缘(鱼央)雌雄中期染色体上进行了FISH定位研究。结果表明,白缘(鱼央)5S rDNA序列无雌雄差异;5S rDNA的保守区序列为117 bp;5S rDNA和45S rDNA分别被定位于白缘(鱼央)的性染色体和第5号染色体上。同时从GenBank中获得了22种鱼的5S rDNA,运用DNAman软件构建了23种鱼的系统发育树,对白缘(鱼央)的进化地位进行了初步研究。本研究为阐明白缘(鱼央)在鱼类系统进化中的位置、重复序列在脊椎动物性染色体上的分布状况以及与性别决定与分化的关系,提供了资料积累和分析依据。     

The Global Distribution of Diapycnal Mixing and Mixing Coefficient Tensor in the Upper 2000m Ocean from Argo Observations

Diapycnal Mixing (DM) within the upper 2000m of the global ocean is calculated by a fine-scale parameterization using the multiyear-mean density gridded product that created by employing all the Argo float observations to date through the recently published equation of seawater TEOS-10. The geographic distribution of Argo-derived DM derived in this study is spatial-dependent and varies with latitude and depth. The magnitude and pattern of DM is favorably validated by comparisons with previous studies. Furthermore, the mixing coefficient tensor K is calculated and analyzed. Components of the tensor fitting for the geopotential coordinate models are also presented. It is found that the tensor components in horizontal direction, K_xx and K_yy, have similar magnitude and distribution pattern. In the vertical, K_zz is enhanced over regions with rough topography and strong wind (e.g., Westerly region), suggesting agreement with previous estimates. This work presents a scheme to estimate the DM and mixing coefficient tensor using Argo observations, and offers a useful Argo-based mixing product for the purpose of promoting the study and modeling of ocean circulation and other processes.     

不同降水天气系统自然降水特征及火箭人工增雨潜力分析

统计分析了1981~2000年20年中15种降水天气系统影响下河北地区自然降水特征,并对火箭人工增雨的潜力进行了初步分析。统计分析表明:西来槽类、高空低涡类、冷锋、切变线和副高后部等天气系统是影响河北地区的主要降水系统,其降雨量和降雨日数占到了90%以上;不同的天气系统在不同季节对降水的贡献有所不同,其中西来槽类的降雨量和降雨日数均居首位,开展人工增雨催化作业机会最多;夏季降水系统最强,云水资源最为丰富,人工增雨潜力很大,是开展火箭人工增雨催化作业的最佳季节,春秋两季增雨潜力明显比夏季小,冬季最小;倒槽、副高后部、台风低压、高空低涡类和气旋类等系统最强,日降雨量和单位面积降雨量明显比其它系统大,尤其对蓄水型火箭增雨作业十分有利。     

附着基粗糙度等因素与海藻密度的相关性研究

海藻附着基表面粗糙度与孢子附着密度有密切的相关性;已经由许多研究证实。但是海藻苗的密度是否仍然受到附着基粗糙度的影响;还缺乏明确的结论。本文以尼龙和亚克力两种材料作为海藻附着基;悬挂于自然海区中使海藻孢子附着并萌发生长;用以研究附着基表面粗糙度对4种海藻密度的影响。结果显示;多管藻、尾孢藻、点叶藻、硬毛藻这4种海藻;在不具沟槽的光滑面;密度显著低于任何有沟槽的粗糙面;但粗糙度不同的附着基之间比较;对海藻密度的影响并不显著。室内培育的海带苗;在尼龙上的密度与粗糙度成正相关;但是将海带苗移至海上培育后;不再与粗糙度有相关性;可能与其假根状固着器下海后的发育有关。本文还试验了海泥、灭活海泥和低栖硅藻3种沉积物与附着基粗糙度对海藻密度的影响;结果表明;沉积物和附着基粗糙度;以及它们的交互作用对海藻密度的影响;因附着基的材料和海藻种类而有差别。     

A statistical analysis of the power law and the logarithmic law using wind data from a 164 m tower

The wind speed distribution with height is analysed by using hourly data for a full year recorded at 6 levels of a 164 m tower at Nanjing. A statistical error analysis shows that power law and logarithmic law are best applicable when the wind is strong. For the height range from 16 to 164 m, the power law represents the actual speed distribution better than does the logarithmic law.     

堰塞坝坝体材料渗流特性研究现状及展望

目前,国内外对地震堰塞坝体稳定性的研究较多,但多数为个例研究。在影响坝体稳定性的众因素中,堰塞坝体的材料组成及其渗透特性对坝体破坏模式有重要的决定作用。本文在前人研究的基础上,从渗流的基本规律、数值模拟、室内试验3个方面总结了针对堰塞坝坝体材料渗流的机理和研究方法。重点分析总结了堰塞坝坝体材料渗透性的研究进展,指出几何条件和水力条件共同决定土体发生渗透变形和渗透稳定性。最后分析了目前针对坝体材料渗透研究存在的问题,提出未来可能的研究方向。     

Defining the range of ecological shelter zones in the shore zone of Three Gorges Reservoir,China

To maintaining a health ecological environment in Three Gorges Reservoir (TGR) area, ecological shelter zones (ESZ) need to be built in the TGR shore zone urgently. Based on the implication and function of ESZ, this study first analyzed the relationships amongst three subsystems of the TGR area, including the upstream production-living land, the downstream reservoir water body, and the ESZ. Then a simple and practical hydrological environment model for controlling the eutrophication of the TGR water body was constructed to determine ESZ range of TGR. Additionally, based on the pollutant degradation efficiency of ESZ and the assimilative capacity of reservoir water body, a raster reverse tracking method was proposed to determine the range of ESZ. Thirdly, take Yangdu town in Zhongxian county as a typical region, the ESZ range under three scenarios of different rainfall intensity, degradation efficiency and inflow water quality that corresponding to the three decisive factors of defining ESZ range were discussed. Finally, the statistical laws of the ESZ’s width at each boundary points were discussed to facilitate the ESZ construction projects, and selected standardized rate at 70, 90, and 99 % were labelled as “General”, “Good” and “Excellent” level to represent the performance of the width of the ESZ range. In conclusion, it suggest “Good” level width as basic width of ESZ, and additionally a special protection zone should be put on upstream environment for the extreme large width at the boundary points.