一株分离自太平洋卡罗琳深海海山水域NX-5T菌株的多相分类鉴定

黄杆菌种类多、分布广,但从深海海山环境中发现黄杆菌新物种的报道还没有。2017年我们从西太平洋卡罗琳深海海山水域分离到一株细菌XN-5^T,经16S rRNA基因序列测定表明,该菌与黄杆菌属(Flavobacterium)内系统发育关系最近模式菌株F.degerlachei LMG 21915^T、F.frigoris LMG 21922^T之间的相似性分别为97.5%和97.8%。本研究采用形态学观察、生理生化特征、遗传特征、脂肪酸和极性脂测定等方法,对该菌株进行了详细的多相分类鉴定。结果表明,XN-5^T菌株在16S rRNA基因系统发育、生长温度、淀粉、酪蛋白水解、硝酸盐还原,产α-半乳糖苷酶、α-葡萄糖苷酶、β-葡萄糖苷酶,同化L-阿拉伯糖、D-甘露醇、N-乙酰-氨基葡萄糖、葡萄糖酸钾,发酵蔗糖、阿拉伯糖,以及脂肪酸、极性脂组成等方面,均与相近模式菌株有所差异。综合多相分类鉴定数据,我们判定XN-5^T菌株为Flavobacterium属内的一个新种,命名为Flavobacterium pacifica sp. nov。     

南极菲尔德斯半岛潮间带沉积物细菌的多样性及分离菌产酶测定

摘要:南极菲尔德斯半岛具有多种不同特征的生态地理微环境,如长城科考站、企鹅岛、生物湾与黄金湾流域、半岛南部、碧玉滩等。这些区域因水文地质、动植物分布、人类活动程度等不同而具有明显的生态地理差异。本研究从这些微环境海岸潮间带采集了7份代表性沉积物样品,采用16S rRNA基因高通量测序方法,对其细菌类群的多样性以及环境理化因子的影响进行了比较分析;同时采用常规可培养鉴定方法,对样品的分离菌株产酶状况进行了初步测定。结果表明：整体上看,所有样品的细菌类群主要分布在45门、104纲、442属当中,表现出比较高的多样性。其中,优势菌门Proteobacteria主要分布在半岛南部、碧玉滩和生物湾潮间带沉积物物中,优势菌门Bacteroidetes主要分布在企鹅岛两侧和黄金湾潮间带沉积物,长城站潮间带沉积物的优势门为Firmicutes。相似生态微环境的潮间带沉积物具有不同的优势菌门和纲;而不同生态微环境潮间带沉积物却具相同的优势菌门和纲。有机氮（TON）、NH4+-N、NO3--N、PO43--P、NO2--N含量与企鹅岛两侧、生物湾、黄金湾潮间带沉积物的菌群多样性具有相关性,其中TON对生物湾样品的影响最大,而有机碳（TOC）对所有样品的影响较小。分离菌株的产酶实验表明：在企鹅岛、生物湾、黄金湾等动物频繁出没的潮间带样品中,蕴藏着一批产淀粉酶、酪蛋白酶、脂肪酶、七叶苷酶、过氧化氢酶能力较强菌株,这为今后进一步的应用开发提供了宝贵的低温产酶菌株来源。 关键词: 菲尔德斯半岛; 潮间带沉积物; 细菌类群多样性; 产酶菌株     

闽江口外海域MIS 5和MIS 3的地震地层学特征和古环境

通过闽江口外海域进行的高分辨率浅地震剖面(拖筏)调查,揭示了MIS 6末期以来最大厚度约90 m的沉积地层。研究了MIS 5和MIS 3地震层序的反射波结构特征,基于地震相分析识别沉积地貌类型;结合深海氧同位素海平面变化曲线,阐述了两时期研究区的沉积环境、特殊地貌的分布和体系域阶段;最后简要探讨了海侵视强度(视高度)及其影响因素。在MIS 5,研究区主要为滨浅海环境,在MIS 5.3和MIS 5.1局部发育了赛江水下三角洲、潮流沙脊和滨海沙坝等沉积地貌;在初期和低海面亚期为陆相环境,发育了闽江和赛江河道,分别经南部和东北部向东入海(台湾海峡)。在MIS 3,研究区普遍为海水覆盖,以滨浅海环境为主,局部发育闽江水下三角洲叶瓣和潮流沙脊。MIS 5的海平面远高于MIS 3,但是典型地震剖面的研究表明,前者的海侵视强度(视高度)低于后者,推断这一现象主要与构造沉降有关,沉积物压缩也有一定的影响,这也可以解释南部海区MIS 5.2的古闽江河道要远低于当时海平面的现象。     

宣痹通络膏对急性痛风性关节炎大鼠MyD88表达及踝关节滑膜组织形态的影响

目的：观察宣痹通络膏对急性痛风性关节炎（AGA）模型大鼠髓样分化因子88（MyD88）表达的影响。方法：将60只雄性Wistar大鼠随机分为6组：宣痹通络膏低、中、高剂量组（简称为低、中、高剂量组）,秋水仙碱组,模型组,空白组,每组各10只。于大鼠踝关节局部注射尿酸钠晶体混悬液制备AGA模型,造模后当天开始进行药物干预,连续3 d。观察各组大鼠不同时间点的关节肿胀度,血清及滑膜组织中MyD88蛋白表达水平,踝关节滑膜组织病理形态学变化。结果：关节肿胀度比较,24 h时,模型组较空白组上升;48h时,高剂量组、秋水仙碱组较模型组降低;72 h时,各给药组均较模型组降低,差异均有统计学意义（P<0.05）。与空白组比较,模型组大鼠血清、滑膜组织MyD88含量显著升高（P<0.01）;与模型组比较,各给药组血清MyD88含量以及高、中剂量组和秋水仙碱组滑膜组织MyD88蛋白含量均降低,差异均有统计学意义（P<0.05或P<0.01）。与模型组相比,各药物组的踝关节滑膜组织病理形态得到改善。结论：宣痹通络膏可能通过下调MyD88蛋白表达,从而改善AGA模型大鼠的踝关节滑膜损伤。     

Simulating urban land-use change based on the multi-hierarchal planning–environment interaction process

Urban land-use change is the result of coupling interaction between planning and environment systems. The aim of our study was to construct an effective model to show how the urban land-use changes under the planning–environment interaction system with multi-hierarchy and major function oriented zoning. Combining the Cellular automata (CA) model with logistic regression model, the proposed multi-hierarchal vector CA model (MH-VCA3) was constructed by mining multi-hierarchal land-use transition rules under the planning–environment interaction system. Taking Jiangyin City (China) as an example, we compared the simulated result of the proposed model to those of the well-accepted Logistic CA and traditional multi-level CA models to demonstrate the effectiveness of the consideration of top-down decomposition constraint and bottom-up updating. Furthermore, by simulating the land-use changes under different population regionalization scenarios, we found that in order to form the spatial pattern of “agglomeration in the north and ecology in the south,” the planned population growth at the global hierarchal level should be allocated to the district units according to the law of Central district > Chengxi district > Chengdong district > Chengnan district > Chengdongnan district. The proposed model is expected to provide scientific support for the formulation of urban planning schemes in the future.     

气候变化感知对城市居民冰雪旅游环境责任行为的影响机制——以北京市为例

冰雪旅游是推动冰雪经济与旅游产业高质量融合发展的重要途径,现已成为文旅产业中最具潜力的领域之一。然而在全球气候变化的影响下,冰雪旅游发展面临严峻挑战。文章以北京市为例,运用结构方程模型对冰雪旅游情境中城市居民的气候变化感知、感知价值、旅游意向和环境责任行为等变量间的影响关系及其形成机制进行分析。研究发现：(1)气候变化感知分为气候变化表征和气候变化风险两个维度,且两者显著正向影响冰雪旅游感知价值。同时,气候变化风险显著正向影响旅游者环境责任行为。(2)冰雪旅游感知价值显著正向影响冰雪旅游意向和环境责任行为。(3)冰雪旅游意向显著正向影响环境责任行为。(4)旅游经历在气候变化感知对冰雪旅游意向和环境责任行为的影响关系中具有显著的正向调节作用。本研究对厘清冰雪旅游情境下气候变化感知对游客环境责任行为的复杂影响机理具有重要的理论价值,同时也能够在一定程度上为相关部门把握“后冬奥”黄金机遇,推动冰雪旅游高质量发展提供决策参考。     

Influence of sequential tropical cyclones on phytoplankton blooms in the northwestern South China Sea

Upper ocean responses to the passage of sequential tropical cyclones over the northwestern South China Sea(SCS) in 2011 were investigated using satellite remote sensing data,Argo reanalysis data,and an array of mooring data.We found that the sea surface low temperature region lasted for more than 38 days and two phytoplankton blooms occurred after the passage of sequential tropical cyclones.The upper ocean cooling reached 2-5℃ with a right-side bias was observed along the typhoon track to about 200 km.The maintenance of low temperature region and the two phytoplankton blooms were mainly driven by upwelling and near-inertial turbulence mixing induced by the sequential tropical cyclones.The first phytoplankton bloom appeared on the 7~(th) day after the passage of the three tropical cyclones,and the chlorophyll-a(chl-a) concentration increased by 226%,which may be mainly driven by typhoons induced upwelling.The second phytoplankton bloom occurred on the 30~(th) day,the chl-a concentration increased by290%.Further analysis suggested that only the typhoons with similar characteristics as Nesat and Nalgae can induce strong near-inertial oscillation(NIO).Strong turbulent mixing associated with the near-inertial baroclinic shear instability lasted for 26 days.The measured mean eddy diffusivity in the upper ocean was above 10~(-4) m~2/s after typhoon Nesat.Enhancement of the turbulent mixing in the upper ocean helped to transport nutrient-rich cold waters from the deep layer to the euphotic layer,and is a maj or mechanism for the long-term maintenance of low temperature region as well as the second phytoplankton bloom.     

Three-dimensional mesoscale eddy identification and tracking algorithm based on pressure anomalies

Journal of Oceanology and Limnology - The Kuroshio Extension (KE) is one of the most eddy-energetic regions in the global ocean. However, most mesoscale eddy studies in the region are focused on...     

小秦岭地区金钼多金属矿成矿特征与找矿预测

小秦岭地区是中国重要的金钼矿产基地。以构造-岩浆-成矿系统为指导,综合研究认为区内金钼多金属矿床可分为印支期—燕山期与重熔型花岗岩浆侵入活动有关的金(钼)矿床成矿系列和与深源浅成型花岗岩、碱性岩有关的钼(金钨)矿床成矿系列。金(钼)矿床类型主要包括石英脉型、构造蚀变岩型、糜棱岩型、爆破角砾岩型和复合型等,以前两者为主;钼(金钨)矿床类型主要包括斑岩型、斑岩-矽卡岩型、斑岩-爆破角砾岩型、石英-碳酸岩脉型和构造蚀变岩型等,也以前两者为主。该区三叠世以来共发生过3次岩浆侵入活动,每次都伴有不同程度的钼金矿化作用,并分别对应于不同的钼金矿化高峰期。钼的成矿作用分为印支期(210~220 Ma)和燕山期早白垩世早期(140~145Ma)、早白垩世中晚期(110~138Ma);金的成矿作用分为印支期(约220Ma)和燕山期早白垩世中晚期(约130Ma);钼、金成矿在印支期和燕山期早白垩世中晚期叠合,而在燕山期早白垩世早期分离。整体上,区内岩体与断裂构造控矿特征明显,燕山期金钼成矿作用强烈发育。该区娃娃沟—峪耳沟、峪耳沟—曹家沟、太子坪—葫芦沟—荒地沟与文公岭北坡等地区为金钼多金属矿的有利找矿靶区,并应特别重视中酸性小岩体的含矿性评价。     

Multi-Time Scale Analysis of Runoff at the Yangtze Estuary Based on the Morlet Wavelet Transform Method

Runoff series of the Yangtze River presents an intricate variation tendency under the reinforced influence of human activities.The Morlet Wavelet Transform method has been applied to analyze the annual runoff data from 1950 to 2011 at the Yangtze River Estuary.It can clearly reveal the multi-time scales structure,break point,change and distribution of periodic variation in the different time scales of the runoff series.The main conclusions are that：1） Repeated periodic oscillations accompanied by an extremely large fluctuation are presented in the runoff series with an obvious difference between wet and dry years,and the major periods of the time series are about 3,8,16 and 23 years respectively.Among them,the presented maximum periodic oscillation is 23 years scale.2） In the 23-year time scale,the wet periods are 1950-1958,1969-1980 and 1992-2003,and the dry periods are 1959-1968,1981-1991 and 2004-2011.3） It can be predicted from the view of long time scales that the low annual runoff will likely occur in the near future.