Field trial using bone meal amendments to remediate mine waste derived soil contaminated with zinc, lead and cadmium

Bone meal amendments are being considered as a remediation method for metal-contaminated wastes. In various forms (biogenic, geogenic or synthetic), apatite, the principal mineral constituent of bone, has shown promise as an amendment to remediate metal-contaminated soils via the formation of insoluble phosphates of Pb and possibly other metals. The efficacy of commercially available bovine bone meal in this role was investigated in a field trial at Nenthead, Cumbria with a mine waste derived soil contaminated with Zn, Pb and Cd. Two 5 m² plots were set up; the first as a control and the second, a treatment plot where the soil was thoroughly mixed with bone meal to a depth of 50 cm at a soil to amendment ratio of 25:1 by weight. An array of soil solution samplers (Rhizon SMS^™) were installed in both plots and the soil pore water was collected and analysed for Ca, Cd, Zn and Pb regularly over a period of 2 a. Concurrently with the field trial, a laboratory trial with 800 mm high and 100 mm wide leaching columns was conducted using identical samplers and with soil from the field site.A substantial release of Zn, Pb, Cd and Ca was observed associated with the bone meal treatment. This release was transient in the case of the leaching columns, and showed seasonal variation in the case of the field trial. It is proposed that this effect resulted from metal complexation with organic acids released during breakdown of the bone meal organic fraction and was facilitated by the relatively high soil pH of 7.6–8.0. Even after this transient release effect had subsided or when incinerated bone meal was substituted in order to eliminate the organic fraction, no detectable decrease in dissolved metals was observed and no P was detected in solution, in contrast with an earlier small column laboratory study. It is concluded that due to the relative insolubility of apatite at above-neutral pH, the rate of supply of phosphate to soil solution was insufficient to result in significant precipitation of metal phosphates and that this may limit the effectiveness of the method to more acidic soils.     

Semipermeable membrane devices link site-specific contaminants to effects: Part 1 - Induction of CYP1A in rainbow trout from contaminants in Prince William Sound, Alaska

Extracts from semi-permeable membrane devices (SPMDs) deployed on beaches in Prince William Sound (PWS), Alaska, were used to evaluate if complex contaminant mixtures from different sources can be distinguished by the resulting cytochrome P450 1A (CYP1A) activity in exposed test animals. Deployment sites included canneries, salmon hatcheries, and beaches where lingering oil remains from discharges during the 1964 earthquake or the 1989 Exxon Valdez oil spill. Other sites were selected at random to evaluate region-wide contaminant inputs or were located in salmon streams to evaluate contaminants carried and released by migrating salmon carcasses following reproduction. Following standard deployments of approximately 28 d, an aliquot of the accumulated contaminants was intraperitoneally injected without cleanup into juvenile rainbow trout (Oncorhynchus mykiss). After 2 d and 7 d, the activity of CYP1A was measured by the ethoxyresorufin-o-deethylase (EROD) assay. Exposure to extracts from the oiled sites and one hatchery site with numerous creosote pilings elicited strong EROD responses, whereas fish exposed to salmon stream extracts elicited weak but significant responses during late summer compared to late spring. Responses from the other sites were not significant, indicating contaminants from these sources are unlikely to cause CYP1A induction in resident biota. Rather than simply assessing extant contaminants, this method evaluates the potency of the different sites for bringing about aryl hydrocarbon receptor responses in resident biota.     

Meteorological and runoff time-series characteristics in a small,high-Arctic glaciated basin,Svalbard

This paper examines characteristics of meteorological and runoff time-series collected from the Brøggerbreen glacier basin, Svalbard, during 1991 and 1992. Proglacial discharge and electrical conductivity were monitored at two gauging stations: one immediately downstream of the terminus of Austre Brøggerbreen and another c. 2·5 km downstream, in order to assess the contribution of the intervening proglacial sandur. Meteorological time-series (incident radiation, wind speed and direction, air temperature and precipitation) were monitored on the proglacial sandur. Changes in wind direction, incident radiation receipt and air temperature were used as a basis for separating the time-series into different periods. These periods allowed the relative significance of advective and incident (short-wave) radiative forcing of air temperatures to be determined at diurnal and synoptic time-scales. The analysis shows that incident radiation dominated over advection in the forcing of diurnal variations in air temperature during all the periods. At the synoptic scale, both processes were periodically dominant in forcing air temperature variability. An examination of synoptic charts supports the use of ground level measurements to describe the effect of energy advection upon the synoptic air temperature variability and indicates the role of large-scale circulation patterns in the delivery of energy for ablation under different conditions. Interrelationships between the hydrological and meteorological time-series are then used to characterize the response of the glacierized part of the catchment to meteorological forcing throughout the two ablation seasons. The analyses show that the recession of the snowpack across the proglacial and glacial portions of the basin has an important effect on the catchment contributing area contributing to runoff and the lag between energy inputs and meltwater discharge outputs. © 1998 John Wiley & Sons, Ltd.     

Suspended sediment yield and transfer processes in a small High-Arctic glacier basin,Svalbard

Observations of suspended sediment concentration and discharge at two sites on the proglacial river network draining from a predominantly cold-based, High-Arctic glacier (Austre Brøggerbreen) are described. Analysis of these observations illustrates: (i) the relatively low suspended sediment yield from this basin in comparison with many other glacier basins reported in the open literature; (ii) sustained and possibly increasing availability of suspended sediment to the fluvial system as the ablation season progresses; and (iii) the role of the proglacial sandur as both a sediment source and sink. Field observations coupled with the results of the data analysis are used to make inferences concerning the changing nature and relative importance of sediment sources within the basin. © 1998 John Wiley & Sons, Ltd.     

Exploring multi-model atmospheric GCM ensembles with ANOVA

Analysis of variance (ANOVA) is a powerful statistical technique for making inferences about experiments that are influenced by multiple factors. Whilst common in many other scientific fields, its use within the climate community has been limited to date. Here we review the basis for ANOVA and how, in particular, it can be applied to partition the variance in a multi-model ensemble of Atmospheric General Circulation Model simulations. We examine an ensemble of four AGCMs forced with observed twentieth century sea surface temperatures (SST). We show that the dominant contributions to the total variance of seasonal mean sea level pressure arise from between-model differences (the bias term) and internal noise (the noise term). However, which term is most important varies from region to region. Of particular interest is the interaction term, which describes differences between the models in their responses to common SST forcing. The interaction term is found to be largest over the Indian Ocean (in all seasons), and over the subtropical Northwest Pacific in boreal summer. The differences between the model responses in these regions suggest differences in their simulation of atmospheric teleconnections, with potentially important implications, e.g. for seasonal predictions of the South and East Asian Monsoons. Examination of these differences may lead to an understanding of the reasons why models respond differently to common forcing, and ultimately to improvements in the performance of climate models.     

On the biogeochemical response of a glacierized High Arctic watershed to climate change: revealing patterns,processes and heterogeneity among micro‐catchments

Our novel study examines landscape biogeochemical evolution following deglaciation and permafrost change in Svalbard by looking at the productivity of various micro‐catchments existing within one watershed. It also sheds light on how moraine, talus and soil environments contribute to solute export from the entire watershed into the downstream marine ecosystem. We find that solute dynamics in different micro‐catchments are sensitive to abiotic factors such as runoff volume, water temperature, geology, geomorphological controls upon hydrological flowpaths and landscape evolution following sea level and glacial changes. Biotic factors influence the anionic composition of runoff because of the importance of microbial SO₄^2? and NO₃^? production. The legacy of glaciation and its impact upon sea level changes is shown to influence local hydrochemistry, allowing Cl^? to be used as a tracer of thawing permafrost that has marine origins. However, we show that a ‘glacial signal’ dominates solute export from the watershed. Therefore, although climatically driven change in the proglacial area has an influence on local ecosystems, the biogeochemical response of the entire watershed is dominated by glacially derived products of rapid chemical weathering. Consequently, only the study of micro‐catchments existing within watersheds can uncover the landscape response to contemporary climate change. Copyright © 2014 John Wiley & Sons, Ltd.     

Semipermeable membrane devices link site-specific contaminants to effects: PART II - A comparison of lingering Exxon Valdez oil with other potential sources of CYP1A inducers in Prince William Sound, Alaska

We deployed semipermeable membrane devices (SPMDs) on beaches for 28 days at 53 sites in Prince William Sound (PWS), Alaska, to evaluate the induction potential from suspected sources of cytochrome P450 1A (CYP1A)-inducing contaminants. Sites were selected to assess known point sources, or were chosen randomly to evaluate the region-wide sources. After deployment, SPMD extracts were analyzed chemically for persistent organic pollutants (POPs) and polycyclic aromatic hydrocarbons (PAH). These results were compared with hepatic CYP1A enzyme activity of juvenile rainbow trout injected with the same extracts prior to clean-up for the chemical analyses. Increased CYP1A activity was strongly associated with PAH concentrations in extracts, especially chrysene homologues but was not associated with POPs. The only apparent sources of chrysene homologues were lingering oil from Exxon Valdez, asphalt and bunker fuels released from storage tanks during the 1964 Alaska earthquake, creosote leaching from numerous pilings at one site, and PAH-contaminated sediments at Cordova Harbor. Our results indicate that PWS is remarkably free of pollution from PAH when nearby sources are absent as well as from pesticides and PCBs generally.     

Relationships of soil,plant, and microbial characteristics in silt-clay and sand,tall-formSpartina alterniflora marshes

The relationships between soil texture, plant growth, and anaerobic microbial activity in two tall-formSpartina alterniflora marshes on Sapelo Island, Georgia, were compared. The soil of one marsh was composed of typical silt-clay-sized particles; the soil of the other marsh consisted of >90% sand-sized particles. The two soils supported similar biomasses ofS. alterniflora, however, plants were taller and more robust in the silt-clay-soil than in the sand soil. Total microbial adenosine triphosphate concentrations in the silt-clay and sand soils averaged 5.71 and 1.64 μg per cm³, respectively. Seawater slurries of both soils exhibited potential for microbial sulfate reduction, methanogenesis, and glucose fermentation; rates for the processes averaged 2.03 and 0.33 nmol S-cm³ per h; 1.20 and 0.87 μmol CH₄ per cm³ per h; and 0.04 and 0.12 per min (rate constant) for the sand and silt-clay soils, respectively.     

Chondrichthyans as an umbrella species-complex for conserving South African biodiversity

Conservation surrogates, such as umbrella and flagship species, could help focus South Africa’s limited resources for research and management and enhance the conservation gains from marine protected areas (MPAs). Sharks, rays and chimaeras (Chondrichthyes), which are charismatic and ecologically diverse, are potential umbrella candidates, but tests of the ecological suitability of putative marine umbrella species are lacking. Using baited remote underwater video in and around two MPAs in the Western Cape Province, we assessed the potential of chondrichthyans as an umbrella species-complex by quantifying the relationships and co-occurrence patterns between chondrichthyan abundance and diversity and those of other taxa (primarily teleosts and crustaceans). Sites with abundant chondrichthyans, with catsharks or large sharks (>1 m total length), all had significantly greater abundance and diversity of these other taxa, and associations with species of commercial and conservation interest (e.g. roman Chrysoblephus laticeps). Endemic scyliorhinids (notably dark catshark Haploblepharus pictus) and the broadnose sevengill shark Notorynchus cepedianus also had many strong positive co-occurrences (28% and 21% of interactions, respectively). The puffadder catshark H. edwardsii had the highest centrality of any species, denoting its high connectedness to other taxa. Overall, chondrichthyans, especially the dark and puffadder catsharks and the broadnose sevengill shark, show strong potential as an umbrella species-complex in South Africa.