Development of a coral cDNA array to examine gene expression profiles in Montastraea faveolata exposed to environmental stress

The development of a cDNA array of coral genes and its application to investigate changes in coral gene expression associated with stressful conditions is described. The array includes both well-characterized and previously unidentified coral genes from Acropora cervicornis and Montastraea faveolata. Corals were exposed to either natural or anthropogenic stressors to elicit the expression of stress genes for isolation and incorporation onto the array. A total of 32 genes involved in protein synthesis, apoptosis, cell signaling, metabolism, cellular defense and inflammation were included on the array. Labeled cDNA from coral (Montastraea faveolata) exposed to elevated seawater temperature, salinity and ultraviolet light was tested against the microarray to determine patterns of gene expression associated with each stressor. Carbonic anhydrase, thioredoxin, a urokinase plasminogen activator receptor (uPAR) and three ribosomal genes demonstrated differential expression across all replicates on the array and between replicate colonies. Specific gene expression patterns produced in response to different stressors demonstrate the potential for gene expression profiling in characterizing the coral stress response.     

基于空间句法-GIS的城市轨道交通供需特征研究——以杭州市为例

在轨道交通网建设初期,交通换乘设施与产业的布局直接关系到各站点的运营与使用效率,选择合理的评价方式,在空间上正确评判其供需水平,能为进一步的规划设计提供科学依据。本文以杭州轨道交通一号线为例,通过空间句法模型,为已投入运营的31个站点的空间整合度进行定量化分析研究：在轨道交通站点可达性评价的基础上,应用GIS—AHP方法,整合现状资料,得出轨道交通需求空间分布特征：结合现状调研资料,阐述空间整合度与交通需求相互作用的机制,进一步讨论杭州轨道交通一号线换乘设施布置的策略,对其未来发展提出建议。     

Nutrient inputs from submarine groundwater discharge on the Santiago reef flat, Bolinao, Northwestern Philippines

Submarine groundwater discharge (SGD) on the reef flat of Bolinao, Pangasinan (Philippines) was mapped using electrical resistivity, 222Rn, and nutrient concentration measurements. Nitrate levels as high as 126 μM, or 1-2 orders of magnitude higher than ambient concentrations, were measured in some areas of the reef flat. Nutrient fluxes were higher during the wet season (May-October) than the dry season (November-April). Dissolved inorganic nitrogen (DIN=NO3+NO2+NH4) and soluble reactive phosphorus (SRP) fluxes during the wet season were 4.4 and 0.2 mmoles m(-2) d(-1), respectively. With the increase population size and anthropogenic activities in Bolinao, an enhancement of SGD-derived nitrogen levels is likely. This could lead to eutrophic conditions in the otherwise oligotrophic waters surrounding the Santiago reef flat.     

Biota: sediment partitioning of aluminium smelter related PAHs and pulp mill related diterpenes by intertidal clams at Kitimat, British Columbia

The question of polycyclic aromatic hydrocarbon (PAH) bioavailability and its relationship to specific PAH sources with different PAH binding characteristics is an important one, because bioavailability drives PAH accumulation in biota and ultimately the biochemical responses to the PAH contaminants. The industrial harbour at Kitimat (British Columbia, Canada) provides an ideal location to study the bioavailability and bioaccumulation of sediment hydrocarbons to low trophic level biota. Samples of soft shell clams (Mya arenaria) and intertidal sediment collected from multiple sites over six years at various distances from an aluminium smelter and a pulp and paper mill were analysed for 106 PAHs, plant diterpenes and other aromatic fraction hydrocarbons. Interpretation using PAH source ratios and multivariate data analysis reveals six principal hydrocarbon sources: PAHs in coke, pitch and emissions from anode combustion from the aluminium smelter, vascular plant terpenes and aromatised terpenes from the pulp and paper mill, petroleum PAHs from shipping and other anthropogenic activities and PAHs from natural plant detritus. Harbour sediments predominantly contain either pitch or pyrogenic PAHs from the smelter, while clams predominantly contain plant derived PAHs and diterpenes from the adjacent pulp mill. PAHs from the smelter have low bioavailability to clams (Biota-Sediment Accumulation Factors; BSAFs <1 for pitch and coke; <10 for anode combustion, decreasing to ∼0.1 for the mass 300 and 302 PAHs), possibly due to binding to pitch or soot carbon matrices. Decreases in PAH isomer ratios between sediments and clams likely reflect a combination of variation in uptake kinetics of petroleum PAHs and compound specific metabolism, with the importance of petroleum PAHs decreasing with increasing molecular weight. Plant derived compounds exhibit little natural bioaccumulation at reference sites, but unsaturated and aromatised diterpenes released from resins by industrial pulping processes are readily accumulated by the clams (BSAFs >500). Thus while most of the smelter associated PAHs in sediments may not be bioavailable to benthic organisms, the plant terpenes (including retene, totarol, ferruginol, manool, dehydroabietane and other plant terpenes that form the chemical defence mechanism of conifers) released by pulp mills are bioavailable and possess demonstrated toxic properties. The large scale release of plant terpenes by some of the many pulp mills located in British Columbia and elsewhere represents a largely undocumented risk to aquatic biota.     

Tangleflags North油田的水平井蒸汽驱先导试验

ＴａｎｇｌｅｆｌａｇｓＮｏｒｔｈ Ｌｌｏｙｄｍｉｎｓｔｅｒ砂岩的蒸汽驱规划区，从１９８２年开始进行一次采油。尽管初期日产水平很高，但因水锥的迅速增长使采出的原油不及原油于质储量的１％，这显然是很不经济的。该区域下有大片的水层，原油粘度是１３０００ｍＰａ．ｓ。本文给出了初期可行性研究和１９８７年晚些时候开始的蒸汽驱先导试验结果，并论述了试验过程，包括井的设计思想。     

盐沼潮滩生物动力地貌演变研究进展

盐沼潮滩作为滨海湿地的一个重要类型,为河口海岸地区提供了多种关键的生态服务功能,具有重要的社会、经济与生态价值,其地貌演变规律的认知是海岸带保护修复和科学管理的基础。回顾国内外近一个世纪以来针对潮滩动力地貌过程的研究工作,发现研究热点已逐渐从传统的研究潮滩水-沙-地貌相互作用过渡到探索植物、动物、微生物等生物作用与动力、地貌之间的互馈机制,涉及海岸动力学、地貌学、沉积学、环境生态学等多个学科,是典型的交叉学科前沿研究领域。归纳了盐沼植被与潮滩多种因子的相互作用,总结了常用的盐沼潮滩地貌演变模拟手段及取得的机理认知,并提出今后在生物作用、陆海耦合动力地貌模型研发等方面的科学问题和发展方向。     

准噶尔盆地南缘小渠子背斜T/P 同构造不整合几何学、运动学特征及地质意义

为探讨准噶尔盆地南缘二叠纪-三叠纪盆地构造性质及构造演化过程,笔者对盆地南缘小渠子背斜保存较完整的T/P 不整合进行了几何学、运动学和沉积韵律旋回特征的分析。T/P 不整合具有同构造不整合的特点,表现为不整合之下削蚀、之上超覆,是由于盆地南缘经历晚二叠世-早三叠世区域性挤压作用造成的。通过对小渠子地区深层地质结构的分析,认为晚二叠世-早三叠世的构造演化过程与早石炭世伸展断陷的反转密切相关。     

安徽池州马头铜钼矿主成矿元素分布特征及富集机理

马头铜钼矿床是江南过渡带池州段达到详查级别的矿床。根据该矿床的矿化分布特征、6个钻孔的成矿元素分析结果,结合镜下鉴定分析认为,马头铜钼矿床岩浆热源中心位于15线以南的西山岩体深部,成矿流体运移方向呈南南西→北北东、西山岩体深部→浅部、岩体中心→远离岩体的迁移规律。Cu、Mo、W等金属元素的迁移、沉淀、富集不是某一种因素单独造成的,而是各种物理化学和地质因素共同作用的结果,是不同期次成矿流体产物的叠加。研究区成矿流体至少有2期,一期为富含以钼为主、伴生铜的热液,另一期为以富铜为主的热液。