松江鲈(Trachidermus fasciatus)泛素结合酶E2-D2基因的分子克隆及组织表达分析

泛素结合酶(UbcE2)是蛋白泛素化过程中所必需的酶,在泛素转移和底物的特异性识别方面发挥着重要的作用。本实验利用RACE技术克隆获得了全长为993bp的松江鲈泛素结合酶E2-D2基因cDNA序列(命名为TfUbcE2-D2),其开放阅读框为444bp,编码147个氨基酸。通过SMART预测得知,TfUbcE2-D2含有一个UBCc结构域。同源比对结果表明该基因与其他物种的同源性为92.31%。实时荧光定量PCR显示,TfUbcE2-D2广泛表达于松江鲈各组织,在肾脏中的相对表达量最高,其次为鳃。鳗弧菌(Vibrio anguillarum)刺激后,TfUbcE2-D2在血液、脾脏、肝脏和鳃中表达均上调,其中,脾脏和鳃中的表达量上调约40倍。由以上实验结果推测,TfUbcE2-D2可能参与松江鲈的先天免疫防御。     

复合蛋白源替代鱼粉对大菱鲆生长、体组成和表观消化率的影响

本实验旨在研究谷朊粉、宠物级鸡肉粉、脱脂肉骨粉、豆粕和玉米蛋白粉复合替代0%(对照组,CON)、35%(FM35)、50%(FM50)、65%(FM65)、80%(FM80)鱼粉对大菱鲆(Scophthalmus maximus)生长、体组成和表观消化率的影响。设计5组等氮等能(粗蛋白52%,总能19kJ/g)的饲料,养殖鱼初重(8.63±0.01)g,养殖周期9周。结果显示,替代组体末重、增重率和特定生长率显著低于CON(P0.05),而摄食率和成活率各处理组之间没有显著变化(P0.05)。替代组饲料效率和蛋白质效率随着替代水平的升高而降低,FM65和FM80饲料效率和FM80蛋白质效率显著低于CON(P0.05)。复合蛋白替代鱼粉对鱼体水分、粗蛋白和粗脂肪没有显著影响(P0.05),但替代组鱼体灰分显著高于CON(P0.05)。除FM35干物质表观消化率与CON没有显著差异(P0.05),其他替代组的干物质和粗蛋白表观消化率均显著低于CON(P0.05)。研究表明,该比例复合蛋白源替代鱼粉水平应不超过35%。     

Nonradial eruption of a kinking filament observed from STEREO

On 5 April 2008, a filament at the periphery of an active region was observed by the Extreme Ultraviolet Imager telescope aboard the STEREO-A spacecraft, which showed up as a prominence eruption in the field-of-view from STEREO-B. The filament at STEREO-A 304 Å was first lengthened toward a region with weak overlying magnetic field so evolved as a large-scale one consisting of bright and dark threads twisting with each other, and then the portion below the weak field underwent an eruption. Meanwhile, the corresponding STEREO-B 304 Å prominence threads exhibited a kinking structure and tilting motion, with its center deflecting from the radial direction. By using three-dimension (3D) reconstruction technology, we obtain the 3D topology for the kinked prominence when its apex arrived at 1.4 radii, from which a clockwise rotation of about 90° is found in the course of the eruption. By comparing the 3D structure with the magnetic-field configuration computed by using the Potential-Field Source-Surface (PFSS) model, it is suggested that the filament erupted against the rather weaker than stronger overlying magnetic field, which make it appear to tilt toward one side.     

基于飞机观测资料的石家庄秋季对流层CH4垂直分布特征

为研究石家庄秋季对流层内CH₄垂直分布特征,2018年9月使用“空中国王350”飞机搭载Picarro温室气体在线观测仪和气象要素观测设备,对石家庄上空（600—5500 m）CH₄浓度进行探测。探测期间共飞行7架次,取得7条CH₄浓度廓线数据。结果表明: 石家庄上空近地面层（1000 m以下）CH₄平均浓度与同时间段区域背景站上甸子站CH₄浓度呈显著正相关（r=0.81,P < 0.03）。探测到的CH₄浓度最小值为1898×10^-9摩尔分数,最大值为2219×10^-9摩尔分数,平均浓度为1981×10^-9摩尔分数。观测得到的7条廓线均有较好的一致性,2000 m以上,浓度均随高度呈先增大后减小的趋势。利用后向轨迹模式（HYSPLIT）对探测时段内石家庄上空不同高度层主要气流传输路径进行统计分析表明,600 m高度输送路径较短,CH₄浓度受本地排放影响较大; 3000 m受输送作用影响较大,偏西和西南路径可能将较高CH₄浓度气团输送至石家庄上空; 5000 m气团传输对CH₄浓度影响较小,浓度相对较稳定,对传输路径的变化不敏感。     

攀钢新基地欠固结土层旋挖桩负摩阻力试验

攀钢新基地工程区80%为"昔格达"土回填区,回填土将对桩产生负摩阻力。现场负摩阻力试验表明:回填土上部在堆载情况下会有较大的固结沉降,从而使桩基产生负摩阻力。     

基于遥感数据的青藏高原热环境调查

本文以地球系统科学和地球力学为理论基础, 以遥感(RS)、地理信息系统(GIS)、全球定位系统(GPS)为技术支撑, 利用亚东－格尔木－锡铁山走廊域的地学剖面实测温度数据和青藏高原MODIS遥感影像数据, 分析研究了青藏高原亚东－格尔木－锡铁山走廊域的热环境特征。通过研究表明, 研究区域内热环境总体分布规律为中东部最高, 东南部次之, 西北部最低。     

REE, Mn, Fe, Mg and C, O Isotopic Geochemistry of Calcites from Furong Tin Deposit, South China: Evidence for the Genesis of the Hydrothermal Ore-forming Fluids

The Furong tin deposit in the central Nanling region, South China, consists of three main types of mineralization ores, i.e. skarn-, altered granite- and greisen-type ores, hosted in Carboniferous and Permian strata and Mesozoic granitic intrusions. Calcite is the dominant gangue mineral intergrown with ore bodies in the orefield. We have carried out REE, Mn, Fe, and Mg geochemical and C, and O isotopic studies on calcites to constrain the source and evolution of the ore-forming fluids. The calcites from the Furong deposit exhibit middle negative Eu anomaly (Eu/Eu*= 0.311–0.921), except for one which has an Eu/Eu* of 1.10, with the total REE content of 5.49–133 ppm. The results show that the calcites are characterized by two types of REE distribution patterns: a LREE-enriched pattern and a flat REE pattern. The LREE-enriched pattern of calcites accompanying greisen-type ore and skarn-type ore are similar to those of Qitianling granite. The REE, Mn, Fe, and Mg abundances of calcites exhibit a decreasing tendency from granite rock mass to wall rock, i.e. these abundances of calcites associated with altered granite-type and greisen-type ores are higher than those associated with skarn-type ores. The calcites from primary ores in the Furong deposit show large variation in carbon and oxygen isotopic compositions. The δ¹³C and δ¹⁸O of calcites are −0.4 to −12.7‰ and 2.8 to 16.4‰, respectively, and mainly fall within the range between mantle or magmatic carbon and marine carbonate. The calcites from greisen and altered granite ores in the Furong deposit display a negative correlation in the diagram of δ¹³C versus δ¹⁸O, probably owing to the CO₂-degassing of the ore-forming fluids. From the intrusion to wall-rock, the calcites display an increasing tendency with respect to δ¹³C values. This implies that the carbon isotopic compositions of the ore-bearing fluids have progressively changed from domination by magmatic carbon to sedimentary carbonate carbon. In combination with other geological and geochemical data, we suggest that the ore-forming fluids represent magmatic origin. We believe that the fluids exsolved from fractionation of the granitic magma, accompanying magmatism of the Qitianling granite complex, were involved in the mineralization of the Furong tin polymetallic deposit.     

四川盆地东北部飞仙关组优质储层特征

对四川盆地东北部下三叠统飞仙关组地质、测井和地震数据的分析表明,该区优质储层多由残余鲕粒白云岩构成,储集空间以裂缝-孔隙型为主,属于中等孔隙度高渗透率性质的储层。优质储层测井曲线多具有低伽马、低速度、低密度和高中子孔隙度的"三低一高"特征,地震反射响应特征为中—强反射振幅并和地层顶底面反射形成典型的"亮点",可利用约束稀疏脉冲反演表征储层的纵横向分布。认为地质与地震相结合是预测飞仙关组优质储层的有效方法。     

A fast inversion method for ocean parameters based on dispersion curves with a single hydrophone

The dispersion characteristics of shallow water can be described by the dispersion curves, which contain substantial ocean parameter information. A fast ocean parameter inversion method based on dispersion curves with a single hydrophone is presented in this paper. The method is achieved through Bayesian theory. Several sets of dispersion curves extracted from measured data are used as the input function. The inversion is performed by matching a replica calculated with a dispersion formula. The bottom characteristics can be described by the bottom reflection phase shift parameter P. The propagation range and the depth can be inverted quickly when the seabed parameters are represented by on parameter P. The inversion results improve the inversion efficiency of the seabed parameters. Consequently, the inversion efficiency and accuracy are improved while the number of inversion parameters is decreased and the computational speed of replica is increased. The inversion results have lower error than the reference values, and the dispersion curves calculated with inversion parameters are also in good agreement with extracted curves from measured data; thus, the effectiveness of the inversion method is demonstrated.