Lower Eocene red-clay sedimentation on the George Bligh/Rockall Channel

Core 7710, recovered on the George Bligh/Rockall Channel, is composed of fine grained ocher-colored sediments rich in ferriferous smectite (montmorillonite, beidellite). These sediments were deposited during early Eocene in a shallow marine environment. Their mineralogic, chemical and isotopic constituents indicate subaerial weathering of lower Eocene intermediate igneous rocks (ferrodiorite, marscoiite) eroded from the still-emergent George Bligh and Rockall Banks. Lack of any post Eocene Sediments may be due to major morphologic and hydrologic changes forming sedimentation gaps.     

Freezing as a method of sample preservation for the analysis of dissolved inorganic nutrients in seawater

It is often desirable or necessary to store collected seawater samples prior to analysis for dissolved inorganic nutrients. It is therefore important to establish preservation and storage techniques that will ensure sample integrity and will not alter the precision or accuracy of analysis. We have performed a series of experiments on the storage of nutrient samples collected at the oligotrophic North Pacific benchmark Station ALOHA, using both standard autoanalyses and low-level techniques. Our results reveal that for oligotrophic oceanic waters, the immediate freezing of an unfiltered water sample in a clean polyethylene bottle is a suitable preservation method. This procedure is simple, it avoids potentially contaminating sample manipulations and chemical additions, and it adequately preserves the concentrations of nitrate + nitrite, soluble reactive phosphate, and soluble reactive silicate within a single water sample.     

Distribution and Diversity of Ibla cumingi Darwin [Crustacea, Cirripedia) from the Gulf of Elat (Red Sea)

Abstract. The distribution of Ibla cumingi DARWIN on different types of hard substrata along 30 km of coast in the Gulf of Elat was studied. I. cumingi was found among and underneath Tetraclita squamosa rufotincta , in oyster beds, underneath slabs of beachrock, and in fissures in igneous rocks. In the Tetraclita belt, I. cumingi is most frequent in empty shells of dead Tetraclita while in the oyster bed it is common inside the meshwork made by the oysters. The distribution is regarded as clustered distribution. The density and size of the clusters of I. cumingi is determined by the available sheltered space within the midlittoral zone. The form of the habitat determines the shape of the animals. Those found in the oyster bed and fissures are usually longer than those found in the Tetraclita belt due to the depth of the fissures or to the bigger spaces within the oyster bed. I. cumingi is orientated so that the capitulum with the cirri is projecting towards the open water, and the water current. It is concluded that I. cumingi can withstand the physical stress encountered in a tropical intertidal zone, but that the distribution pattern is caused by predation.     

Comparison of cytochrome P450 in three butterflyfish species: Ecological implications of elevated CYP2B and CYP3A in Chaetodon capistratus

The relationship between cytochrome P450 and feeding on terpenoid-rich gorgonian corals was investigated in a species of tropical butterflyfish and compared with two other sympatric congeners that do not feed on gorgonians. Fish were collected from non-polluted waters in Belize and the levels of two cytochrome P450 isozymes (CYP2B and CYP3A) were immunoquantitated in addition to quantification of total P450. Chaetodon capistratus regularly feeds on gorgonian corals and has higher levels of total hepatic microsomal cytochrome P450 than C. ocellatus or C. striatus. The content of hepatic P450 (0.588–0.794 nmol mg⁻¹) in C. capistratus is among the highest ever reported in teleosts from non-polluted waters and is significantly greater than detected in C. ocellatus or C. striatus. Chaetodon capistratus also had a larger hepatic index (g liver per g fish) and more microsomal protein (mg protein per g liver), factors that translate into 3.3- to 8-fold more total P450 per g fish. Sexual differences in total P450 were observed between male and female C. capistratus, but not among the other species. The contents of proteins detected by immunoassay with polyclonal anti-scup P450B (CYP2B) and anti-human P4503A (CYP3A) were 2- to 10-fold and 2- to 20-fold greater, respectively, in C. capistratus than in the congeneric species. CYP2 and CYP3 gene families in mammals are thought to have evolved partially in response to dietary allelochemicals. These results suggest that these P450 isozymes may also be important in marine teleosts that feed on terpenoid-rich prey.     

The effect of effluent high in chromium on marine sediment aerobic heterotrophic bacteria

A survey of the aerobic heterotrophic bacteria present in sediments at Sawyers Bay, New Zealand, receiving tannery effluent high in Cr, and a control site, indicates that the populations present are different and show seasonal variation. The bacterial population present at the polluted site appears more able to tolerate CrIII at concentrations less than 0·2 μmol ml^?1.     

Wave loading on axisymmetric bodies using axisymmetric hybrid integral equation method

The wave diffraction problem on axisymmetric structures are solved by treating the fluid field as two separate domains. The velocity potential in the inner domain is represented by a 1/r type Green's function whilst that of the outer domain is represented by an eigenfunction expansion. The simple form of the Green's function in the inner domain reduces significantly the computational effort whilst the eigenfunction expansion in the outer domain is able to satisfy the radiation boundary condition completely. The method requires to have elements cover the entire containing boundary. Results for a number of typical structural geometries are presented and discussions are made on the effect of various parameters.     

Rapid visualization of plankton abundance and taxonomic composition using the Video Plankton Recorder

Traditional methods for determining spatial distributions of planktonic taxa involve net, pump, and bottle collections followed by the tedious and time-consuming task of plankton sample analysis. Thus, plankton ecologists often require months or even years to process samples from a single study. In this paper, we present a method that allows rapid visualization of the distribution of planktonic taxa while at sea. Rapid characterization of plankton distributions is essential in the dynamic physical environment, where biological and physical patterns can change quickly. Such a “sample-and-observe” capability is necessary for mapping ephemeral features (such as patches, eddies, jets, plumes) and determining appropriate locations to conduct more localized sampling, including in situ observational studies. We describe the techniques used in imaging the plankton, analyzing the video, and visualizing the data. We present an example of at-sea data analysis conducted aboard R.V. Columbus Iselin on Georges Bank in May 1994 and visualizations of the 3-dimensional distribution of selected planktonic taxa in a 2 × 2 km × 90 m volume of seawater. A video of the image processing and visualization is included on the CD-ROM accompanying this volume and is an essential part of this paper.     

The Japanese medaka (Oryzias latipes) model: applicability for investigating the immunosuppressive effects of the aquatic pollutant benzo[a]pyrene (BaP)

Despite the fact that BaP is a carcinogen, mammalian immunosuppressant, and ubiquitous aquatic pollutant, knowledge regarding the effects of BaP on the immune system of fish is still lacking. To begin to fill this gap, studies were conducted in medaka to examine the effects and mechanisms by which BaP exposure might alter host immunocompetence. Fish, exposed by IP injection of BaP (2-600 microg/g BW), were examined after 48 h for effects upon immune function and CYP1A expression/activity. Benzo[a]pyrene, at a concentration below that which increased levels of CYPIA expression/activity (2 microg BaP/g BW) suppressed lymphocyte proliferation. Concentrations of BaP at 20 and 200 microg/g BW. suppressed antibody-forming cell (AFC) numbers, superoxide production, and host resistance against bacteria. In contrast, exposure to the low affinity aryl hydrocarbon receptor (AhR) agonist, benzo[e]pyrene (BeP), neither induced CYP1A expression nor altered immune function. Given the lack of immunosuppressive effects produced by BeP, and the fact that exposure to the AhR antagonist (and CYP1A inhibitor) alpha-naphthoflavone (ANF) ameliorated the suppressive effects of BaP upon AFC numbers, the AhR pathway (including CYP1A-mediated production of reactive BaP metabolites) appears important in mediating BaP-induced immunotoxicity in fish, as in mammals. In the past, the medaka has proven a successful model for assessing carcinogenic agents. These studies have demonstrated its utility for also determining the immunosuppressive effects of an important aquatic contaminant.