Rapid, oxygen-dependent microbial Mn(II) oxidation kinetics at sub-micromolar oxygen concentrations in the Black Sea suboxic zone

Microbial Mn(II) oxidation kinetics in response to oxygen concentration were assessed in suboxic zone water at six sites throughout the Black Sea. Mn(II) oxidation rates increased asymptotically with increasing oxygen concentration, consistent with Michaelis-Menten enzyme kinetics. The environmental half-saturation constant, K_E, of Mn(II) removal (oxidation) varied from 0.30 to 10.5 μM dissolved oxygen while the maximal environmental rate, V_E−max, ranged from 4 to 50 nM h⁻¹. These parameters varied spatially and temporally, consistent with a diverse population of enzymes catalyzing Mn oxide production in the Black Sea. Coastally-influenced sites produced lower K_E and higher V_E−max constants relative to the Western and Eastern Gyre sites. In the Bosporus Region, the Mn(II) residence time calculated using our K_E and V_E−max values with 0.1 μM oxygen was 4 days, 25-fold less than previous estimates. Our results (i) indicate that rapid Mn(II) oxidation to solid phase Mn oxides in the Black Sea’s suboxic zone is stimulated by oxygen concentrations well below the 3-5 μM concentration reliably detected by current oceanographic methods, (ii) suggest the existence of multiple, diverse Mn(II)-oxidizing enzymes, (iii) are consistent with shorter residence times than previously calculated for Mn(II) in the suboxic zone and (iv) cast further doubt on the existence of proposed reactions coupling solid phase Mn oxide production to electron acceptors other than oxygen.     

Development of large-area land cover and forest change indicators using multi-sensor Landsat imagery: Application to the Humber River Basin,Canada

Monitoring ecological indicators is important for assessing impacts of human activities on ecosystems. A means of identifying and applying appropriate indicators is a prerequisite for: environmental assessment; better assessment and understanding of ecosystem health; elucidation of biogeochemical trends; and more accurate predictions of future responses to global change, particularly those due to anthropogenic disturbance. The challenge is to derive meaningful indicators of change that capture the complexities of ecosystems yet can be monitored consistently over large areas and across time. In this study, methods for monitoring indicators of land cover (LC) and forest change were developed using multi-sensor Landsat imagery. Mapping and updating procedures were applied to the Humber River Basin (HRB) in Newfoundland and Labrador, one of four test sites in Canada selected for testing the development of national-scale methods. Procedures involved unsupervised clustering and labeling of baseline imagery, followed by image-to-image spectral clustering to derive binary change masks within which new LC types were classified for non-baseline imagery. Updated maps were compatible with the baseline map and reflected change in LC for three time periods: 1976–1990, 1990–2001, and 2001–2007. From the LC products, several change indicators were quantified including: forest depletion, forest regeneration, forest change, net forest change, and annual rates of change. The procedures were validated using field plots to assess the accuracy of the 2007 LC product (74.2% for 10 LC classes) and change classes observed from 2001 to 2007 (87.8% for four change classes: depletion, regeneration, non-treed class no change, and treed class no change). Methods were considered to be highly efficient and operationally feasible over large areas spanning multiple Landsat scenes. Specific results for the test site provided trend information supporting land and resource management in the HRB region.     

Bio-volatilization of polonium: Results from laboratory analyses

Polonium, like other elements in the Group VI Oxygen series (S, Se, Te), has the potential to form volatile alkyl derivatives. This may be evident in its pervasive radioactive excesses in the atmosphere and coastal waters, and its deficiency in surface open ocean waters. We present evidence for the formation of volatile polonium species. The first evidence comes from duplicating experiments that proved the existence of volatile tellurium, its Group VI congener. Cultures of bread mold at room temperature spiked with polonium tracers showed a significant and reproducible loss of about 0.5% per day of volatile polonium species. In another set of experiments, between 30–50% loss of²¹⁰Po was observed from Floridan groundwater when nitrogen was bubbled through it over durations of 5–30 minutes. Polonium volatility is highly relevant for biogeochemical studies because it may provide a natural radiochemical tracer for recycling of similar volatile sulfur and other Group VI metal species between the geosphere, atmosphere, and hydrosphere.     

Distribution of diffuse flow megafauna in two sites on the Eastern Lau Spreading Center, Tonga

Hydrothermal vent environments are characterized by large gradients of toxic chemicals and high temperatures, which play a significant role in defining species’ distributions. We used high-resolution imagery and spatially explicit in-situ physico-chemical measurements analyzed within a Geographic Information System (GIS) in order to characterize the spatial relations among different groups of megafauna, temperature, and chemistry within two discrete vent communities (40 and 50 m²) on the Eastern Lau Spreading Center (ELSC). Chemical (sulfide and O₂ concentrations) and temperature data were obtained from approximately 75 different locations within each community using in-situ instruments. All data were integrated into a GIS, which served as a visualization tool and enabled the data to be analyzed in a spatial context. Our results confirm the importance of abiotic variables in defining the distributions of some fauna and elucidate several biological associations that are consistent between the two communities. The provannid snail, Alviniconcha spp., appears to actively avoid temperatures above 32–46 °C and/or sulfide concentrations exceeding approximately 260 μM. Slightly higher average sulfide concentrations and temperatures were measured among aggregations of Ifremeria nautilei compared to aggregations of the mussel Bathymodiolus brevior; however, the presence of mixed aggregations of the two species indicates an overlap in requirements. The brachyuran crab, Austinograea spp., was consistently observed directly on symbiont-containing species, particularly Alviniconcha spp. The solitary snail, Eosipho desbruyeresi, was rarely observed on biological substrata, but was often (60% of its population at the most active site) within 5 cm of symbiont-containing fauna, indicating a tolerance and preference for proximity to areas of high productivity. Densities and coverage of species differed substantially between the two communities despite high species overlap. Symbiont-containing species covered much larger areas at the more hydrothermally active site, ABE1, while shrimp and anemones occurred in relatively higher densities within the less-active site, TM1. This is the first study to thoroughly characterize realized distributions of megafauna at vent sites along the ELSC.     

Laser‐induced melting experiments: Simulation of short‐term high‐temperature impact processes

This study introduces an experimental approach using direct laser irradiation to simulate the virtually instantaneous melting of target rocks during meteorite impacts. We aim at investigating the melting and mixing processes of projectile (iron meteorite; steel) and target material (sandstone) under idealized conditions. The laser experiments (LE) were able to produce features very similar to those of impactites from meteorite craters and cratering experiments, i.e., formation of lechatelierite, partial to complete melting of sandstone, and injection of projectile droplets into target melts. The target and projectile melts have experienced significant chemical modifications during interaction of these coexisting melts. Emulsion textures, observed within projectile‐contaminated target melts, indicate phase separation of silicate melts with different chemical compositions during quenching. Reaction times of 0.6 to 1.4 s could be derived for element partitioning and phase‐separation processes by measuring time‐depended temperature profiles with a bolometric detector. Our LE allow (i) separate melting at high temperatures to constrain primary melt heterogeneities before mixing of projectile and target, (ii) quantification of element partitioning processes between coexisting projectile and target melts, (iii) determination of cooling rates, and (iv) estimation of reaction times. Moreover, we used a thermodynamic approach to calculate the entropy gain during laser melting. The entropy changes for laser‐melting of sandstone and iron meteorite correspond to shock pressures and particle velocities produced during the impact of an iron projectile striking a quartz target at a minimum impact velocity of ~6 km s^?1, inducing peak shock pressures of ~100 GPa in the target.     

Subsurface deformation of experimental hypervelocity impacts in quartzite and marble targets

Two impact cratering experiments on nonporous rock targets were carried out to determine the influence of target composition on the structural mechanisms of subsurface deformation. Projectiles of 2.5 mm diameter were accelerated to ~5 km s⁻¹ and impacted onto blocks of marble or quartzite. Subsurface deformation was mapped and analyzed on the microscale using thin sections of the bisected craters. Additionally, both experiments were modeled and the calculated strain zones underneath the craters were compared to experimental deformation features. Microanalysis shows that the formation of radial, tensile, and intragranular cracks is a common response of both nonporous materials to impact cratering. In the quartzite target, the subsurface damage is additionally characterized by highly localized deformation along shear bands with intense grain comminution, surrounded by damage zones. In contrast, the marble target shows closely spaced calcite twinning and cleavage activation. Crater diameter and depth as well as the damage lens underneath the crater are unexpectedly smaller in the marble target compared to the quartzite target, which is in contradiction to the marble's much weaker compressive and tensile strengths. However, numerical models result in craters that are similar in size as well as in strain accumulation at the end of transient crater formation, indicating that current models should still be viewed cautiously when compared to experimental details.     

Heavy metal distribution in Newark Bay sediments

Bottom sediments have been analysed from Newark Bay, New Jersey, for zinc, lead, cadmium, and mercury. The bottom sediment analyses show that the bay sediments are enriched over background levels up to 65 times for zinc, 128 times for lead, 180 times for cadmium, and 155 times for mercury. Analyses of the metal distribution and of available tidal hydraulic data indicate the metal concentrations are due to a combination of source and tidal hydraulics. Dye tracer experiments gathering Lagrangiantype data appear to yield the best result in the investigation of metal trasport phenomena.     

Determing the effects of El Niño-Southern Oscillation events on coastal water quality

The importance of the El Niño-Southern Oscillation (ENSO) on regional-scale climate variability is well recognized although the associated effects on local weather patterns are poorly understood. Little work has addressed the ancillary impacts of climate variability at the community level, which require analysis at a local scale. In coastal communities water quality and public health effects are of particular interest. Here we describe the historical influence of ENSO events on coastal water quality in Tampa Bay, Florida (USA) as a test case. Using approximate randomized statistics, we show significant ENSO influences on water quality particularly during winter months, with significantly greater fecal pollution levels during strong El Niño winters and significantly lower levels during strong La Niña winters as compared to neutral conditions. Similar significant patterns were also noted for El Niño and La Niña fall periods. The success of the analysis demonstrates the feasibility of assessing local effects associated with large-scale climate variability. It also highlights the possibility of using ENSO forecasts to predict periods of poor coastal water quality in urban region which local agencies may use to make appropriate prepations.