全文获取类型
收费全文 | 73474篇 |
免费 | 24387篇 |
国内免费 | 48090篇 |
专业分类
测绘学 | 5718篇 |
大气科学 | 27196篇 |
地球物理 | 21199篇 |
地质学 | 53452篇 |
海洋学 | 19327篇 |
天文学 | 2746篇 |
综合类 | 8765篇 |
自然地理 | 7548篇 |
出版年
2024年 | 262篇 |
2023年 | 820篇 |
2022年 | 2231篇 |
2021年 | 2799篇 |
2020年 | 3517篇 |
2019年 | 6974篇 |
2018年 | 7519篇 |
2017年 | 7066篇 |
2016年 | 7460篇 |
2015年 | 6367篇 |
2014年 | 6387篇 |
2013年 | 7029篇 |
2012年 | 6584篇 |
2011年 | 6497篇 |
2010年 | 6301篇 |
2009年 | 5703篇 |
2008年 | 4832篇 |
2007年 | 4780篇 |
2006年 | 4141篇 |
2005年 | 4014篇 |
2004年 | 3758篇 |
2003年 | 3477篇 |
2002年 | 3187篇 |
2001年 | 3075篇 |
2000年 | 2885篇 |
1999年 | 3490篇 |
1998年 | 3147篇 |
1997年 | 3144篇 |
1996年 | 2698篇 |
1995年 | 2470篇 |
1994年 | 2148篇 |
1993年 | 2004篇 |
1992年 | 1627篇 |
1991年 | 1281篇 |
1990年 | 1097篇 |
1989年 | 916篇 |
1988年 | 814篇 |
1987年 | 575篇 |
1986年 | 490篇 |
1985年 | 366篇 |
1984年 | 379篇 |
1983年 | 244篇 |
1982年 | 261篇 |
1981年 | 186篇 |
1980年 | 154篇 |
1979年 | 140篇 |
1978年 | 66篇 |
1977年 | 61篇 |
1971年 | 54篇 |
1958年 | 50篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
941.
942.
利用牙鲆鳃细胞系分离和培养淋巴囊肿病毒 总被引:1,自引:4,他引:1
本文利用牙鲆鳃细胞系进行了养殖牙鲆淋巴囊肿病毒的分离及培养 ,并通过电镜对培养细胞中淋巴囊肿病毒的形态及感染循环进行了初步研究。将病鱼的淋巴囊肿组织无菌滤液接种牙鲆细胞系 ,细胞出现了明显的细胞病变 ( Cytopathic effect,CPE)。电镜观察在培养细胞的胞质中有病毒的包涵体 ,胞质中散在 6角形、5角形或圆形的病毒粒子 ,大小为 10 0~ 140 nm之间。在感染细胞的线粒体中也存在大量的病毒颗粒。 相似文献
943.
利用RAPD和AFLP标记 ,以一回交家系 [(Miyagi×Hiroshima)×Miyagi]为作图群体 ,构建了太平洋牡蛎的遗传连锁图谱。用经过筛选的 3 3个RAPD引物和 1 1个AFLP引物组合 ,对父母本和 80个子代个体进行了遗传分析。共得到母本分离标记 1 93个 ,其中 1 44个符合1∶1孟德尔分离规律。父本分离标记 1 5 6个 ,其中 1 1 1个符合 1∶1孟德尔分离规律。雌性框架图包括 99个遗传标记 ,定位在 1 2个连锁群中 ,覆盖 985 2cM ,标记间平均间隔 1 1 3cM。另外有 3个三联体 ,7个连锁对 ,图谱共覆盖 1 1 6 5 7cM。雄性框架图包括 72个遗传标记 ,分布在 8个连锁群 ,覆盖 81 1cM ,标记间平均间隔 1 2 7cM。另外有 4个三联体 ,3个连锁对 ,图谱共覆盖93 1 8cM。 相似文献
944.
Diagnosis of iridovirus in large yellow croaker by PCR 总被引:1,自引:0,他引:1
A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection. 相似文献
945.
近几十年来,许多沿海国家都开展了大规模的海岸带调查工作,并对海岸带的泥沙运动和地貌演变的某些规律进行了深人研究。但是,对于有潮海滩不同地貌部位上的泥沙运动特征及其控制因素,以及不同地貌部位上,反映泥沙群体运动结果的堆积体运动规律等的现场调查,至今报道不多。实际上,有潮海滩并非单调一致,海滩不同地貌部位上的泥沙运动规律和地貌发育规律,都有极大的、甚至本质上的差异。研究这些差异性,对于我们深入了解海岸带泥沙运动和地貌演变的规律,是有所裨益的。
白沙口位于山东半岛南岸,五垒岛湾之西(见图1) ,湾口朝南,面对黄海。湾内堆积地貌发育。一条巨大的湾口沙咀,自常家庄南岬角向西延伸,逼近海阳所南部的角滩。沙咀的北面为泻湖,白沙滩河注入其中。所谓白沙口,实指泻湖的出口,也为一潮汐通道。由于陆源物质长期倾泻入海,出口处岸线呈扇形向海突出,形成白沙口潮汐三角洲。退潮后,三角洲上一片白沙,沙咀、沙洲等堆积地貌非常发育。它们在海浪、潮流,和河流径流等各种动力因素的交互作用下,活动性很强,其位置变化无常,给航运和水工建筑等带来许多困难。1971-1974年,中国科学院海洋研究所和山东省水文总站协作,在本区进行了系统调查,曾写了专门调查报告。本文试图根据其中的部分现场观测资料,对本区海岸带泥沙运动和地貌演变的一般特征、差异性,及其与动力因素之间的关系等问题进行初步探讨。 相似文献
946.
947.
Koji Suzuki Akira Hinuma Hiroaki Saito Hiroshi Kiyosawa Hongbin Liu Toshiro Saino Atsushi Tsuda 《Progress in Oceanography》2005,64(2-4):167
To verify the hypothesis that the growth of phytoplankton in the Western Subarctic Gyre (WSG), which is located in the northwest subarctic Pacific, is suppressed by low iron (Fe) availability, an in situ Fe fertilization experiment was carried out in the summer of 2001. Changes over time in the abundance and community structure of phytoplankton were examined inside and outside an Fe patch using phytoplankton pigment markers analyzed by high-performance liquid chromatography (HPLC) and flow cytometry (FCM). In addition, the abundance of heterotrophic bacteria was also investigated by FCM. The chlorophyll a concentration was initially ca. 0.9 μg l−1 in the surface mixed layer where diatoms and chlorophyll b-containing green algae (prasinophytes and chlorophytes) were predominant in the chlorophyll biomass. After the iron enrichment, the chlorophyll a concentration increased up to 9.1 μg l−1 in the upper 10 m inside the Fe patch on Day 13. At the same time, the concentration of fucoxanthin (a diatom marker) increased 45-fold in the Fe patch, and diatoms accounted for a maximum 69% of the chlorophyll biomass. This result was consistent with a microscopic observation showing that the diatom Chaetoceros debilis had bloomed inside the Fe patch. However, chlorophyllide a concentrations also increased in the Fe patch with time, and reached a maximum of 2.2 μg l−1 at 5 m depth on Day 13, suggesting that a marked abundance of senescent algal cells existed at the end of the experiment. The concentration of peridinin (a dinoflagellate marker) also reached a maximum 24-fold, and dinoflagellates had contributed significantly (>15%) to the chlorophyll biomass inside the Fe patch by the end of the experiment. Concentrations of 19′-hexanoyloxyfucoxanthin (a prymnesiophyte marker), 19′-butanoyloxyfucoxanthin (a pelagophyte marker), and alloxanthin (a cryptophyte marker) were only incremented a few-fold increment inside the Fe patch. On the contrary, chlorophyll b concentration reduced to almost half of the initial level in the upper 10 m water column inside the Fe patch at the end of the experiment. A decrease with time in the abundance of eukaryotic ultraphytoplankton (<ca. 5 μm in size), in which chlorophyll b-containing green algae were possibly included was also observed by FCM. Overall, our results indicate that Fe supply can dramatically alter the abundance and community structure of phytoplankton in the WSG. On the other hand, cell density of heterotrophic bacteria inside the Fe patch was maximum at only ca. 1.5-fold higher than that outside the Fe patch. This indicates that heterotrophic bacteria abundance was little respondent to the Fe enrichment. 相似文献
948.
Observations of fluid mud were made in the lower North Passage of the Yangtze Estuary in February 2000, on 10 -11 August 2000, on 30 - 31 August 2000 (after two strong typhoons), on 21 - 24 August 2000 (neap tide) and on 3 -6 September 2000 (mean tide) respectively. In situ data show that the fluid mud in this area consists of fine cohesive sediment (median size 7.23 μm). The formation and movement of fluid mud varied during the neap-spring and flood-ebb tidal cycle. Observations suggest that fluid mud phenomena in this area may be categorised in a three-fold manner as slack water, storm and saltwedge features. The thickness of the fluid mud layer of slack water during the neap tide ranged from 0.2 to 0.96 m, whereas during the mean tide, the thickness ranged from 0.17 to 0.73 m, and the thickness of the fluid mud layer was larger during slack water than at the flood peak. Shoals cover an area of 800 km^2 with a water depth smaller than 5 m. Erosion of these extensive intertidal mudflats due to storm action provides an abundant sediment source. This is particularly significant in this estuary when the tidal level is lower than 5 m. The lower North Passage is a typical zone of saltwater wedging, so the saltwedge fluid mud has the most extensive spatial range in the estuary. 相似文献
949.
利用分光光度计比色法测定了温度、pH值和Hg^2+等9种金属离子对体长24.2cm±0.7cm仿刺参(Apostichopus japonicus)消化道中蛋白酶活性的影响。结果表明,蛋白酶的最适温度为40℃,对热不稳定,最适pH为7.2。Hg^2+,Mn^2+,Ag^+,Pb^2+,Ba^2+,Ca^2+对蛋白酶的活性有抑制作用,抑制作用由强至弱依次为Hg^2+〉Ag^+〉Mn^2+〉Pb^2+〉Ca^2+〉Ba^2+,其中Hg^2+和Ag^+与对照差异极显著(P〈0.01),表现出极强的抑制作用;Cu^2+,Zn^2+,Mg^2+则对酶活有一定的促进作用,其促进作用大小依次为Mg^2+〉Zn^2+〉Cu^2+,Mg^2+与对照差异显著(P〈0.05)。 相似文献
950.
The Barra Nova inlet, in south Portugal, is known to migrate progressively southeastwards under wave action. The morphodynamics
of this system during a representative year suggests that this long-term evolution is dependent on a seasonal behavior of
the tidal inlet which can be described through a three-stage model of post-storm, transition and extended fair-weather conditions.
Processes involved in this evolution indicate that the historical migration of the Barra Nova is not dependent on the longshore
drift forcing constricting the channel on the updrift coast, but rather on the adjustment of the system to a major erosion
of the downdrift coast during short storm events. 相似文献