Antioxidative capacity and enzyme activity in Haematococcus pluvialis cells exposed to superoxide free radicals

The antioxidative capacity of astaxanthin and enzyme activity of reactive oxygen eliminating enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) were studied in three cell types of Haematococcus pluvialis exposed to high concentrations of a superoxide anion radical (O₂⁻). The results show that defensive enzymes and astaxanthin-related mechanisms were both active in H. pluvialis during exposure to reactive oxygen species (ROS) such as O₂⁻. Astaxanthin reacted with ROS much faster than did the protective enzymes, and had the strongest antioxidative capacity to protect against lipid peroxidation. The defensive mechanisms varied significantly between the three cell types and were related to the level of astaxanthin that had accumulated in those cells. Astaxanthin-enriched red cells had the strongest antioxidative capacity, followed by brown cells, and astaxanthin-deficient green cells. Although there was no significant increase in expression of protective enzymes, the malondialdehyde (MDA) content in red cells was sustained at a low level because of the antioxidative effect of astaxanthin, which quenched O₂⁻ before the protective enzymes could act. In green cells, astaxanthin is very low or absent; therefore, scavenging of ROS is inevitably reliant on antioxidative enzymes. Accordingly, in green cells, these enzymes play the leading role in scavenging ROS, and the expression of these enzymes is rapidly increased to reduce excessive ROS. However, because ROS were constantly increased in this study, the enhance enzyme activity in the green cells was not able to repair the ROS damage, leading to elevated MDA content. Of the four defensive enzymes measured in astaxanthin-deficient green cells, SOD eliminates O₂⁻, POD eliminates H₂O₂, which is a by-product of SOD activity, and APX and CAT are then initiated to scavenge excessive ROS.     

Zoospore-derived monoecious gametophytes in Undaria pinnatifida (Phaeophyceae)

The annual life cycle of the brown seaweed Undaria pinnatifi da(Harvey)Suringer comprises a macroscopic diploid sporophyte stage and a microscopic haploid gametophyte stage.In 2011,an unusual zoospore-derived monoecious gametophyte isolate(designated as line 10-5-3)of U.pinnatifi da was observed.To understand this phenomenon,a comprehensive screening of eighty-two previously identifi ed male gametophyte cultures,isolated from three randomly selected cultivars(lines 10,7,and 5)was performed.Thirty-six of the isolates developed both antheridia and oogonia on the same fi lamentous fragment in a standard gametogenesis test(SGT: 18°C,60 μmol photons/(m 2·s)).Selfi ng of the monoecious gametophyte or crossing it with a normal male gametophyte both gave rise to morphologically normal sporophytic offspring.However,crossing resulted in a much higher fertilization rate(89.7%).The hybrid and selfed sporophytic offspring were grown to maturity in fl ow tanks at an ambient temperature of 10–18°C over a period of 69 days.Active zoospores were released from both types of mature sporophylls.The majority of these developed into male gametophytes,while 15%–20% developed into the observed monoecious structures on the same fi lament.Using PCR amplifi cation it was found that all the monoecious gametophyte isolates and the sporophytic offspring resulting from the selfi ng and crossing lacked the femalelinked microsatellite sequence(a part of the locus Up-AC-2A8,GenBank accession No.AY738602.1),indicating their male nature.U.pinnatifi da is an invasive species in some regions and the implications of the above fi ndings for this species in nature are briefl y discussed.     

中国古代常规日食记录的整理分析

收集并分析了公元前8世纪到公元15世纪的中国古代密集、简略而公式化的日食记录。基于这些记录整理成一个包括938条日食记录的计算机可读形式的“常规日食记录表”。该表给出每次记录的原日期、公历日期、当时首都、所属朝代以及一部分记录所载的进一步详情。对该表的构成作了介绍,并对这些历史记录进行了初步的分析和归纳。     

真鲷的营养需要及配合饲料的研究初报

本文综述了真鲷的营养需要，研究了不同植物蛋白源对真鲷生长的影响，并在此基础上设计了三种饲料配方，试验结果表明Ｉ号饲料配方的增长率最高。     

十堰及邻区地震构造环境浅析

研究了十堰及邻区区域构造背景和地球物理场分布特点，分析了区域性地震构造与有感地震和中强地震活动的关系。结果表明：区内中强地震活动与北西西向、北北西向断裂及北东-北北东向断裂密切有关；5级左右地震活动发生在大型沉降区内次级凸起与凹陷的交接带、现代构造活动的盆地或地槽、莫霍面斜坡扭曲部位；6级以上地震发生在现代地壳隆起边缘的沉降区一侧或大型凸起与凹陷区的过渡部位。     

海底沉积物的基本地声结构与地声模型

为准确建立海底地声模型,本文探讨地声模型的基本组成和基本结构。通过样品实验室测量,分析南海海底表层沉积物的密度、孔隙度与声速随着埋深变化的关系,得出海底实际存在的低声速表面–声速缓慢变化类型、低声速表面–声速增大类型、高声速表面–声速缓慢变化类型和高声速表面–声速增大类型4种典型地声结构;对比钻探测量,分析黄海海底沉积物的密度、孔隙度与声速随埋深变化关系,得出海底地声模型分层特征与地声结构组合特征。研究表明,地声模型可以归结为4种基本地声结构的组合,通过与底层海水声速、同层内声速剖面以及与上层海底沉积物下表面声速的比较,可以建立各种海底地声模型;基于实验室测量法建立的地声模型可以作为参考地声模型,但需要考虑实际海底温度和压力梯度以及海底沉积物的频散特性等,借助于声速比校正法和频散性理论模型进行计算及修正。     

Spatiotemporal analysis of COVID-19 risk in Guangdong Province based on population migration

Journal of Geographical Sciences - Population migration, especially population inflow from epidemic areas, is a key source of the risk related to the coronavirus disease 2019 (COVID-19) epidemic....     

对抗航空磁探的磁诱饵模拟指标分析及度量方法

针对航空磁探的威胁,潜艇可通过施放能够模拟其空中磁场特性的装置(磁诱饵)进行对抗,在对磁诱饵进行研究时,需要对其模拟需求进行分析。针对此问题,在对目前常用磁探仪工作原理及优缺点进行分析的基础上建立了基于标量磁探仪的航空磁探潜模型,以所建立的航空磁探潜模型为基础,分析了对抗航空磁探时磁源所需满足的模拟需求,并给出对抗效能评估度量方法及指标:磁场功率比、磁场方向可调性指标及有效面积指标,为潜艇空中磁场模拟方法研究的开展提供了基础。     

装载GPCTD传感器的海翼水下滑翔机观测数据实时质量控制

Profiles observed by Sea-Wing underwater gliders are widely applied in scientific research. However, the quality control(QC) of these data has received little attention. The mismatch between the temperature probe and conductivity cell response times generates erroneous salinities, especially across a strong thermocline. A sensor drift may occur owing to biofouling and biocide leakage into the conductivity cell when a glider has operated for several months. It is therefore critical to design a mature real-time QC procedure and develop a toolbox for the QC of Sea-Wing glider data. On the basis of temperature and salinity profiles observed by several Sea-Wing gliders each installed with a Sea-Bird Glider Payload CTD sensor, a real-time QC method including a thermal lag correction, Argo-equivalent real-time QC tests, and a simple post-processing procedure is proposed. The method can also be adopted for Petrel gliders.     

关于垂准仪校准过程中铅垂度的研究

对垂准仪校准过程中同轴度和一测回垂准测量标准偏差进行了分析,并提出了铅垂 p 角的概念.