Molecular cloning of the heat shock protein 20 gene from Paphia textile and its expression in response to heat shock

P. textile is an important aquaculture species in China and is mainly distributed in Fujian, Guangdong, and Guangxi Provinces. In this study, an HSP20 c DNA designated Pt HSP20 was cloned from P. textile. The full-length c DNA of Pt HSP20 is 1 090 bp long and contains a 5′ untranslated region(UTR) of 93 bp, a 3′ UTR of 475 bp, and an open reading frame(ORF) of 522 bp. The Pt HSP20 c DNA encodes 173 amino acid residues and has a molecular mass of 20.22 k Da and an isoelectric point of 6.2. Its predicted amino acid sequence shows that Pt HSP20 contains a typical α-crystallin domain(residues 77–171) and three polyadenylation signal-sequences at the C-terminus. According to an amino acid sequence alignment, Pt HSP20 shows moderate homology to other mollusk s HSPs. Pt HSP20 m RNA was present in all of the test tissues including the heart, digestive gland, adductor muscle, gonad, gill, and mantle, with the highest concentration found in the gonad. Under the stress of high temperature, the expression of Pt HSP20 m RNA was down-regulated in all of the tissues except the adductor muscle and gonad.     

末次冰盛期以来日本海浮游植物生产力和群落结构变化

生物标志物法已经被广泛应用于重建海洋浮游植物生产力和群落结构变化。本文利用这种方法重建了日本海末次冰盛期以来浮游植物生产力和群落结构变化。结果显示,生物标志物含量在末次冰期最盛期21～19 ka和14～4 ka时有高值,而在19～14 ka、4～0 ka有低值。末次冰期最盛期时生物标志物的高含量是由于分层导致的底层水缺氧,生物标志物得到较好保存的结果,而此时日本海盐度很低,使颗石藻相对含量很低;19～14 ka陆源输入减少,日本海生产力水平较低,不断增加的盐度使浮游植物群落结构由硅藻、甲藻占优转变为颗石藻占优;14～4 ka对马暖流流量的加大提高了日本海的初级生产力,同时高营养盐利于硅藻、甲藻的生长,使颗石藻的相对比例减小;4 ka以后随冬季风的增强和黑潮减弱,对马暖流减弱,供应的营养盐减少,日本海生产力水平降低,使颗石藻的相对比例增大,硅藻、甲藻的相对比例减小。     

Investigations into the thermal non-equilibrium of W UMa-type contact binaries

Traditionally, some physical details(e.g., magnetic braking, energy transfer, angular momentum loss, etc.) have to be taken into consideration during investigations into the evolution of contact binaries. However, the real evolutionary processes which usually contain several of these physical mechanisms are very complicated as a result of strong interaction between components. To avoid dealing with these factors, a linear relationship is applied to the temperatures of components. It is found that the higher the mass ratio(M_2/M_1) of a contact system, the weaker the deviation from thermal equilibrium.On this basis, a variation trend of fill-out factor(f) changing with mass ratio can be inferred, which is consistent with observations. Moreover, if we stick to this point of view, it should be natural that the number of semi-detached binaries in the predicted broken-contact phase of relaxation oscillations is less than the number in the contact phase.     

安国药材产业发展的经济文化基础*

在区域产业经济发展过程中,地缘优势、经济规律、文化传统都是不容忽视的重要因素。文章以安国市的药材产业为研究实例,通过解释中心集聚的“空间拉力”所孕育的对外交流的潜力,揭示“交易成本机制”在市场交易中的刺激作用,以及分析安国药市网络管理的特定形式,对药材产业区域发展的地理、历史、经济和文化因子作了综合性研究。     

Using triple oxygen isotopes and oxygen-argon ratio to quantify ecosystem production in the mixed layer of northern South China Sea slope region

Quantifying the gross and net production is an essential component of carbon cycling and marine ecosystem studies.Triple oxygen isotope measurements and the O_2/Ar ratio are powerful indices in quantifying the gross primary production and net community production of the mixed layer zone,respectively.Although there is a substantial advantage in refining the gas exchange term and water column vertical mixing calibration,application of mixed layer depth history to the gas exchange term and its contribution to reducing indices error are unclear.Therefore,two cruises were conducted in the slope regions of the northern South China Sea in October 2014(autumn) and June 2015(spring).Discrete water samples at Station L07 in the upper 150 m depth were collected for the determination of δ~(17)0,δ~(18)O,and the O_2/Ar ratio of dissolved gases.Gross oxygen production(GOP) was estimated using the triple oxygen isotopes of the dissolved O_2,and net oxygen production(NOP) was calculated using O_2/Ar ratio and O_2 concentration.The vertical mixing effect in NOP was calibrated via a N_2O based approach.GOP for autumn and spring was(169±23) mmol/(m~2·d)(by O_2) and(189±26) mmol/(m~2·d)(by O_2),respectively.While NOP was 1.5 mmol/(m~2·d)(by O_2) in autumn and 8.2 mmol/(m~2·d)(by O_2) in spring.Application of mixed layer depth history in the gas flux parametrization reduced up to 9.5% error in the GOP and NOP estimations.A comparison with an independent O_2 budget calculation in the diel observation indicated a26% overestimation in the current GOP,likely due to the vertical mixing effect.Both GOP and NOP in June were higher than those in October.Potential explanations for this include the occurrence of an eddy process in June,which may have exerted a submesoscale upwelling at the sampling station,and also the markedly higher terrestrial impact in June.     

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The chemical changes of DOM from black waters to coastal marine waters by HPLC combined with ultrahigh resolution mass spectrometry

How dissolved organic matter (DOM) undergoes chemical changes during its transit from river to ocean remains a challenge due to its complex structure. In this study, DOM along a river transect from black waters to marine waters is characterized using an offline combination of reversed-phase high performance liquid chromatography (RP-HPLC) coupled to electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS), as well as tandem ESI-FTICR-MS. In addition, a water extract from degraded wood that mainly consists of lignins is used for comparison to the DOM from this transect. The HPLC chromatograms of all DOM samples and the wood extract show two major well-separated components; one is hydrophilic and the other is hydrophobic, based on their elution order from the C₁₈ column. From the FTICR-MS analysis of the HPLC fractions, the hydrophilic components mainly contain low molecular weight compounds (less than 400 Da), while the hydrophobic fractions contain the vast majority of compounds of the bulk C₁₈ extracted DOM. The wood extract and the DOM samples from the transect of black waters to coastal marine waters show strikingly similar HPLC chromatograms, and the FTICR-MS analysis further indicates that a large fraction of molecular formulas from these samples are the same, existing as lignin-like compounds. Tandem mass spectrometry experiments show that several representative molecules from the lignin-like compounds have similar functional group losses and fragmentation patterns, consistent with modified lignin structural entities in the wood extract and these DOM samples. Taken together, these data suggest that lignin-derived compounds may survive the transit from the river to the coastal ocean and can accumulate there because of their refractory nature.     

Petrology,mineralogy, and oxygen isotope compositions of aluminum‐rich chondrules from CV3 chondrites

Bulk major element composition, petrography, mineralogy, and oxygen isotope compositions of twenty Al‐rich chondrules (ARCs) from five CV3 chondrites (Northwest Africa [NWA] 989, NWA 2086, NWA 2140, NWA 2697, NWA 3118) and the Ningqiang carbonaceous chondrite were studied and compared with those of ferromagnesian chondrules and refractory inclusions. Most ARCs are marginally Al‐richer than ferromagnesian chondrules with bulk Al₂O₃ of 10–15 wt%. ARCs are texturally similar to ferromagnesian chondrules, composed primarily of olivine, pyroxene, plagioclase, spinel, Al‐rich glass, and metallic phases. Minerals in ARCs have intermediate compositions. Low‐Ca pyroxene (Fs_0.6–8.8Wo_0.7–9.3) has much higher Al₂O₃ and TiO₂ contents (up to 12.5 and 2.3 wt%, respectively) than that in ferromagnesian chondrules. High‐Ca pyroxene (Fs_0.3–2.0Wo_33–54) contains less Al₂O₃ and TiO₂ than that in Ca,Al‐rich inclusions (CAIs). Plagioclase (An_77–99Ab_1–23) is much more sodic than that in CAIs. Spinel is enriched in moderately volatile element Cr (up to 6.7 wt%) compared to that in CAIs. Al‐rich enstatite coexists with anorthite and spinel in a glass‐free chondrule, implying that the formation of Al‐enstatite was not due to kinetic reasons but is likely due to the high Al₂O₃/CaO ratio (7.4) of the bulk chondrule. Three ARCs contain relict CAIs. Oxygen isotope compositions of ARCs are also intermediate between those of ferromagnesian chondrules and CAIs. They vary from ?39.4‰ to 13.9‰ in δ¹⁸O and yield a best fit line (slope = 0.88) close to the carbonaceous chondrite anhydrous mineral (CCAM) line. Chondrules with 5–10 wt% bulk Al₂O₃ have a slightly more narrow range in δ¹⁸O (?32.5 to 5.9‰) along the CCAM line. Except for the ARCs with relict phases, however, most ARCs have oxygen isotope compositions (>?20‰ in δ¹⁸O) similar to those of typical ferromagnesian chondrules. ARCs are genetically related to both ferromagnesian chondrules and CAIs, but the relationship between ARCs and ferromagnesian chondrules is closer. Most ARCs were formed during flash heating and rapid cooling processes like normal chondrules, only from chemically evolved precursors. ARCs extremely enriched in Al and those with relict phases could have had a hybrid origin (Krot et al. 2002) which incorporated refractory inclusions as part of the precursors in addition to ferromagnesian materials. The occurrence of melilite in ARCs indicates that melilite‐rich CAIs might be present in the precursor materials of ARCs. The absence of melilite in most ARCs is possibly due to high‐temperature interactions between a chondrule melt and the solar nebula.     

快速射电暴的数据统计

快速射电暴于2007年首次在脉冲星巡天的历史数据中被发现,是一种在射电波段观测到的具有色散量的单个强脉冲,其特征为持续时间仅为若干毫秒,峰值流量密度可达到央斯基量级。类似于射电脉冲星的单个脉冲,但其色散显著超过同一视线方向上银河系内星际介质的预期最大值。对截止2018年6月帕克斯望远镜、绿岸望远镜、阿雷西博射电望远镜、UTMOST望远镜和ASKAP望远镜已探测到的52例快速射电暴进行了观测量统计分析,根据模型扣除银河系星际介质导致的色散量后,快速射电暴平均色散量为584.5 pc·cm^-3,暗示着快速射电暴来自河外。通过最佳拟合估算红移幂律分布谱dN/dF_obs=4.14±1.30×F■sky^-1·day^-1。根据分析结果预计,FAST望远镜使用19波束接收机后,其多科学目标同时巡天在经过一年时间内能发现大约10个快速射电暴,将有效地扩大样本数量,为快速射电暴研究提供重要信息。