BAC end sequencing of Pacific white shrimp <Emphasis Type="Italic">Litopenaeus vannamei</Emphasis>: a glimpse into the genome of Penaeid shrimp

Little is known about the genome of Pacific white shrimp (Litopenaeus vannamei). To address this, we conducted BAC (bacterial artificial chromosome) end sequencing of L. vannamei. We selected and sequenced 7 812 BAC clones from the BAC library LvHE from the two ends of the inserts by Sanger sequencing. After trimming and quality filtering, 11 279 BAC end sequences (BESs) including 4 609 paired- ends BESs were obtained. The total length of the BESs was 4 340 753 bp, representing 0.18% of the L. vannamei haploid genome. The lengths of the BESs ranged from 100 bp to 660 bp with an average length of 385 bp. Analysis of the BESs indicated that the L. vannamei genome is AT-rich and that the primary repeats patterns were simple sequence repeats (SSRs) and low complexity sequences. Dinucleotide and hexanucleotide repeats were the most common SSR types in the BESs. The most abundant transposable element was gypsy, which may contribute to the generation of the large genome size of L. vannamei. We successfully annotated 4 519 BESs by BLAST searching, including genes involved in immunity and sex determination. Our results provide an important resource for functional gene studies, map construction and integration, and complete genome assembly for this species.     

基于水利益共享的跨境流域水资源多目标分配研究——以澜沧江-湄公河为例

以水利益共享代替分水的理念有利于充分发挥水资源效益和减少区域矛盾冲突,但由于缺乏具体可实施的分配模式一直停留在设想阶段。基于水利益共享理念,建立跨境流域水资源多目标分配指标体系,并结合澜沧江-湄公河流域跨境水资源利用现状及需求,提出澜沧江-湄公河流域跨境水资源多目标分配模型。为基于水利益共享的跨境水资源多目标分配提供了具有充分可操作性的指标体系和分配模型,有助于推进跨境流域水利益共享的实施,实现区域双边或多边在水资源利用上的共赢和发展目标。     

Application of saddlepoint approximation in reliability analysis of dynamic systems

The application of the saddlepoint approximation to reliability analysis of dynamic systems is investigated. The failure event in reliability problems is formulated as the exceedance of a single performance variable over a prescribed threshold level. The saddlepoint approximation technique provides a choice to estimate the cumulative distribution function (CDF) of the performance variable. The failure probability is obtained as the value of the complement CDF at a specif ied threshold. The method requires computing the saddlepoint from a simple algebraic equation that depends on the cumulant generating function (CGF) of the performance variable. A method for calculating the saddlepoint using random samples of the performance variable is presented. The applicable region of the saddlepoint approximation is discussed in detail. A 10-story shear building model with white noise excitation illustrates the accuracy and effi ciency of the proposed methodology.     

〈史记－历术甲子篇〉探讨

〈史记－历术甲子篇〉给出了一个７６年周期中各历年的年首，月数和冬至、但由于没有推大小月。闰月和２４节气的方法。仍得不到完整的历谱、本义分析了〈历术甲子篇〉的基本条件，由此出发推导出原文的全部数据．分析原文的数学逻辑，同时发现原文所置的闰年也同样符合这一方法．本文按同样原则设想了置大小月，置２４节气，置闰月的方法，这样方可得到完整的历谱．本文还对〈历术甲子篇〉的实用性和误差进行了讨论．指出简单易行的改进方法．并对中国古代历法的某些原则提出批评。     

生物生态防治虾病研究

本文探索虾病防治新途径,采用低值海藻江蓠和对虾混养的生态学方法,江蓠吸收水中二氧化碳进行光合作用,产生大量氧气;同时吸收有机物的分解物 NH_3—N,加速有机物分解。减少 H_2S 积累;从而消除水中紧迫因子威胁,大大减少池水自污及病害,起到抑菌、抑虫、净化水质作用,提高成活率,试验圹对虾成活率达95.1%,对照圹56.6%。试验结果表明,虾病防治效果显著,经济效益明显。     

The Magnetic Topological Structure and Energy of the 2B/X2 Flare in NOAA 8100

In this paper, we reconstruct the finite energy force-free magnetic field of the active region NOAA 8100 on 4 November 1997 above the photosphere. In particular, the 3-D magnetic field structures before and after a 2B/X2 flare at 05:58 UT in this region are analyzed. The magnetic field lines were extrapolated in close coincidence with the Yohkoh soft X-ray (SXR) loops accordingly. It is found that the active region is composed of an emerging flux loop, a complex loop system with differential magnetic field shear, and large-scale, or open field lines. Similar magnetic connectivity has been obtained for both instants but apparent changes of the twisting situations of the calculated magnetic field lines can be observed that properly align with the corresponding SXR coronal loops. We conclude that this flare was triggered by the interaction of an emerging flux loop and a large loop system with differential magnetic field shear, as well as large-scale, or open field lines. The onset of the flare was at the common footpoints of several interacting magnetic loops and confined near the footpoints of the emerging flux loop. The sheared configuration remained even after the energetic flare, as demonstrated by calculated values of the twist for the loop system, which means that the active region was relaxed to a lower energy state but not completely to the minimum energy state (two days later another X-class flare occurred in this region).     

怒江迪庆州陡坡耕地的改良和持续利用研究

滇西北的怒江、迪庆两州地处云南省高寒高山峡谷区，地形破碎而陡峻，耕地稀缺，陡坡种植业占有很高的比重。由于人口的增长，陡坡耕地仍将作为该地区的主要耕地长期存在。这种传统的耕种方式已对环境产生了深刻的负效应。本文根据其生存条件和利用现状就通过改良以取得持续利用的方向和途径作了初步探讨。     

中亚与中国西北地区陷落型前陆盆地的构造样式及成因分析

中亚与中国西北地区的多数中—新生代沉积盆地都表现为山前（指造山带或活动隆起带前缘）地带先行沉降,然后逐步向外扩展;早—中侏罗世沉降幅度最大,普遍形成未补偿的深湖—半深湖沉积,沉积范围遍及造山带,构成一准平原化的泛盆地沉积时期。盆地早期的构造样式均为半地堑式箕状凹陷,晚期均发生方式不同的的构造反转,形成全反转的逆冲推覆和半反转的正或逆反转构造。这些特征及区域构造背景共同说明,这一地区中—新生代盆地初始沉降不是起因于板块俯冲引发的挠曲作用,而是因造山带或活动隆起带前缘地带克拉通地块的重力陷落所引起,这样形成的盆地可称为陷落型前陆盆地。     

Lateral continuity of basement seismic reflections in 15 Ma ultrafast-spreading crust at ODP Site 1256

The Ocean Drilling Program (ODP) initiated drilling at Site 1256D in the Guatemala Basin, about 1,000 km off the East Pacific Rise to penetrate plutonic rocks, anticipated to be relatively shallow in this region, formed at an ultra-fast spreading rate. IODP Expedition E312 successfully drilled into gabbros at ~1,150 m in basement. Multi-channel seismic traces show weak laterally coherent sub-basement reflections at borehole depths. Synthetic reflectivity seismograms were computed using a Ricker wavelet and impedance profiles from borehole sonic logs. These seismograms show significant sub-basement amplitude peaks. A zero-offset vertical seismic profile, shot on E312, was processed to investigate the authenticity of these reflections and their relationship to borehole geology. A dual scheme of the median filtering and F–K dip filtering was used. Tests with synthetic seismograms indicate the approach is effective at reasonable SNR levels. Downgoing energy is clearly identified but negligible upgoing energy is visible over random noise. These results indicate that lava flows and igneous contacts in upper ocean crust have significant topography on lateral scales less than the Fresnel Zone (~300 m) due to igneous and tectonic processes.     

Environmental Geochemistry and Acid Mine Drainage Evaluation of an Abandoned Coal Waste Pile at the Alborz-Sharghi Coal Washing Plant,NE Iran

In this paper, an abandoned waste coal pile, which is resulted from Alborz-Sharghi coal washing plant, NE of Iran was mineralogically and geochemically characterized to evaluate pyrite oxidation, acid mine drainage (AMD) generation, and trace element mobility. After digging ten trenches and vertical sampling, a quantitative method including the atomic absorption test, and the quality-based methods including optical study were carried out for determination of pyrite fractions in the waste pile. The geochemical results revealed that the fraction of remaining pyrite increased with depth, indicating that pyrite oxidation is limited to the shallower depths of the pile which were confirmed by variations of sulfate, pH, EC, and carbonate with depth of the pile. To evaluate the trend of trace elements and mineralogical constituents of the waste particles, the samples were analyzed by using XRD, ICP-MS, and ICP-OES methods. The results showed the secondary and neutralizing minerals comprising gypsum have been formed below the oxidation zone. Besides, positive values of net neutralization potential indicated that AMD generation has not taken in the waste pile. In addition, variations of trace elements with depth reveal that Pb and Zn exhibited increasing trends from pile surface toward the bottom sampling trenches while another of them such as Cu and Ni had decreasing trends with increasing depth of the waste pile.