文蛤抗癌多肽对N-乙酰-β-D氨基葡萄糖苷酶活力的影响

从文蛤体中分离纯化得到了抗癌多肽,研究该多肽对N-乙酰-β-D-氨基葡萄糖苷酶(NAGase)活力的影响,结果显示文蛤抗癌多肽对该酶有明显的抑制作用.在本文中,我们将深入地研究其抑制作用机理和抑制效应类型,结果表明:随着文蛤多肽加入量的增大,该酶活力呈指数下降,测得导致酶活力下降50%的抑制剂浓度(IC50)为112.9 μg/cm3,该多肽对酶的抑制作用是一种可逆过程,抑制机理表现为混合型抑制类型,对游离酶(E)的抑制常数(KI)和酶-底物络合物的抑制常数(KIS)分别为77.03和697.44μg/cm3,说明文蛤抗癌多肽与游离酶的结合导致酶活力的丧失,明显的强于对酶-底物络合物的抑制效应,底物的存在对该酶被文蛤抗癌多肽抑制作用起了保护作用.     

冬季北黄海表层海水pCO_2分布及其影响因素探讨

根据2006年12月在北黄海走航连续观测所得pCO2 数据,结合水文、化学和生物等要素的同步观测资料,对该海域pCO2分布及其影响因素进行了探讨.结果表明:冬季北黄海表层海水pCO2测值在203～683 μatm之间,平均值为408 μatm.辽南沿岸流及其影响区域是大气CO2汇区;山东半岛以北沿岸,122°E以西受渤海环流输送,黄河悬浮颗粒物影响的高浑浊度区域是大气CO2的一个强源区(最高值达到683 μatm);而占据北黄海大部的黄海混合水以及北黄海整体上是大气CO2的弱源.冬季北黄海表层海水pCO2分布主要受控于海水温度、碳酸盐体系平衡和生物活动,即温度越高pCO2越高、DIC 越高pCO2越高、叶绿素含量越高pCO2越低.其中辽南沿岸流及其影响区域生物活动的影响最为显著.而山东半岛以北沿岸的高浑浊度区域水体性质具有特殊性,较高的 pCO2受控于高的DIC浓度以及陆岸的影响.     

一种针对规则冲突的规则集语义比较算法

应用DFD(Diverse Firewall Design)方法进行防火墙规则集设计时,需使用规则集语义比较算法,判断多个规则集是否语义一致。规则冲突是导致规则集语义不一致的主要原因。因此,需要研究针对规则冲突的规则集语义比较算法。而现有算法时空复杂度较高,性能低下。文中提出了1种基于关键数据包选取映射的规则集语义比较算法(KPCM)。KPCM算法针对规则冲突选取关键数据包,并通过比较这些数据包在不同规则集中的处理动作,判断这些规则集是否语义一致。理论分析和测试表明,KPCM算法时空性能均优于现有算法。     

Sensitivity of penman-monteith reference crop evapotranspiration in Tao’er River Basin of northeastern China

A non-dimensional relative sensitivity coefficient was employed to predict the responses of reference crop evapotranspiration （ET0） to perturbation of four climate variables in Tao＇er River Basin of the northeastern China. Mean monthly ET0 and yearly ET0 from 1961 to 2005 were estimated with the FAO-56 Penman-Monteith Equation. A 45-year historical dataset of average monthly maximum/minimum air temperature, mean air temperature, wind speed, sunshine hours and relative humidity from 15 meteorological stations was used in the analysis. Results show that： 1） Sensitivity coefficients of wind speed, air temperature and sunshine hours were positive except for those of air temperature of Arxan Meteorological Station, while those of relative humidity were all negative. Relative humidity was the most sensitive variable in general for the Tao＇er River Basin, followed by sunshine hours, wind speed and air temperature. 2） Similar to climate variable, monthly sensitivity coefficients exhibit large annual fluctuations. 3） Sensitivity coefficients for four climate variables all showed significant trends in seasonal/yearly series. Also, sensitivity coefficients of air temperature, sunshine hours and wind speed all showed significant trends in spring. 4） Among all sensitivity coefficients, the average yearly sensitivity coefficient of relative humidity was highest throughout the basin and showed largest spatial variability. Longitudinal distribution of sensitivity coefficients for air temperature, relative humidity and sunshine hours was also found, which was similar to the distribution of the three climate variables.     

城镇地籍测量实施方案探讨

以地籍测量为研究对象,阐述了现代地籍调查的特征,结合湖北省安陆市城镇地籍调查项目的实际工作和成功经验,对地籍调查工作进行了各个方位的理性分析,提出了现代地籍测量完善可行的工作实施方案,较好地解决了地籍调查工作中的一些实际矛盾。并对实施方案中如何提高工作效率进行了有益的探讨。     

遥感信息协同智能网格模型设计及实现

为了给普通用户提供一个自动化、智能化的遥感信息处理平台,以OGSA(Open Grid Services Architecture) 为基础,结合多智能体技术,提出了基于协同智能网格的遥感信息处理模型RSCIG(Remote Sensing Cooperative Intelligent Grid);设计了RSCIG的体系结构,详述了如何高效准确地协同现有的网格服务以完成所面临的任务;实现了一个RSCIG体系结构下的遥感信息处理原型系统.     

Total lipid and fatty acid composition of eight strains of marine diatoms

Fatty acid composition and total lipid content of 8 strains of marine diatoms (Nitzschia frustrula, Nitzschia closterium, Nitzschia incerta, Navicula pelliculosa, Phaeodactylum tricornutum, Synedra fragilaroides) were examined. The microalgae were grown under defined conditions and harvested at the late exponential phase. The major fatty acids in most strains were 14∶0 (1.0%–6.3%), 16∶0 (13.5–26.4%), 16∶1n−7 (21.1%–46.3%) and 20∶5n−3 (6.5%–19.5%). The polyunsaturated fatty acids 16∶2n−4, 16∶3n−4, 16∶4n−1 and 20∶4n−6 also comprised a significant proportion of the total fatty acids in some strains. The characteristic fatty acid composition of diatoms is readily distinguishable from those of other microalgal groups. Significant concentration of the polyunsaturated fatty acid 20∶5n−3 (eicosapentaenoic acid) was present in each strain, with the highest proportion in B222 (19.5%). Project supported by the Hi-Tech “863” Programs of the China Ministry of Science and Technology (863-819-02-01).     

Characteristics of Sulfide Removal by Hydrogenovibrio thermophilus Strain TT in Mariculture System

To biologically inhibit the production of highly toxic sulfide in the aquaculture area, one bacterium, designated as Hydrogenovibrio thermophilus strain TT, was isolated from sediment in Jiaozhou Bay, China. The strain was found to be microaerobic mixtrophic, employing both carbon dioxide and organic carbons, e.g. citrate, lactate, glucose, and peptone as carbon sources. When it oxidized the sulfide and thiosulfate, oxygen was the optimal electron acceptor, followed by nitrite and nitrate, which benefited to removal of sulfide under low-oxygen mariculture environment. In addition, the strain TT exhibited remarkable tolerance of sulfide and thiosulfate in seawater, and it was able to oxidize 18.52 mmol L~(-1) S~(2-) in 12 h or 45 mmol L~(-1) S_2O_3~(2-) in 24 h at initial pH 7.0-9.0, 30-40℃. Therefore, this strain showed a quite promising application for biological remediation of sulfide-contaminated mariculture system.     

Purification and Characterization of a Novel Hydrolase That Can Specifically Degrade the Polysaccharide Isolated from Green Seaweed Ulva prolifera

The extracellular polysaccharide hydrolase-producing strain EP-1 was isolated from seawater and identified as Paenibacillus pabuli. Furthermore, a homogeneous extracellular polysaccharide hydrolase from Paenibacillus pabuli EP-1 was purified by combining ion-exchange chromatography and size exclusion chromatography with a purification fold of 90.69 and recovery of 16.23%. Characterization of the purified polysaccharide hydrolase revealed a molecular mass of 38 k Da and optimum activity at 45℃ and pH 6.0. The polysaccharide hydrolase maintained its stability within a wide range of pH(3.0–12.0) and thermal stability when the temperature was below 50℃. The presence of Hg²⁺, Fe²⁺, Mn²⁺, Co²⁺ and SDS notably decreased hydrolase activity, and organic solvents such as formaldehyde, acetone, DMF and acetonitrile completely inhibited hydrolase activity. The purified hydrolase had no activity on agar, carrageenan, gellan gum, sodium alginate, or starch, but effectively hydrolyzed the polysaccharide from Ulva prolifera. The Km and Vmax values of this hydrolase were 43.84 mg m L^-1 and 4.33 mg m L^-1 min^-1, respectively. The sequence analysis with quantitative time-of-flight mass spectrometry indicated that the hydrolase was an endoglucanase.     

Rockfall seismic features analysis based on in situ tests:frequency,amplitude, and duration

In the seismic event classification,determining the seismic features of rockfall is significantly important for the automatic classification of seismic events because of the huge amount of raw data recorded by seismic stations in continuous monitoring. At the same time, the rockfall seismic features are still not completely understood.This study concentrates on the rockfall frequency content, amplitude(ground velocity), seismic waveform and duration analysis, of an artificial rockfall test at Torgiovannetto(a former quarry in Central Italy). A total of 90 blocks were released in the test, and their seismic signals and moving trajectories were recorded by four tri-axial seismic stations and four cameras, respectively. In the analysis processing,all the artificial rockfall signal traces were cut separately and the seismic features were extracted individually and automatically. In this study, the relationships between a) frequency content and impacted materials, b) frequency content and the distance between block releasing position and seismic station(source-receiver distance) were discussed. As a result, we found that the frequency content of rockfall focuses on 10-60 Hz and 80-90 Hz within a source-receiver distance of 200 m, and it is well correlated with impacted material and source-receiver distance. To evaluate the difference between earthquake and rockfall, 23 clear earthquake signals recorded in a seven month-long continuous seismic monitoring, carried out with the four seismic stations, were picked out, according to the Italian national earthquakes database(INGV). On these traces we performed the same analysis as in the artificial rockfall traces, and two parameters were defined to separate rockfall events from earthquake noise. The first one, the amplitude ratio, is related to the amplitude variation of rockfall between two stations and is greater than that of earthquakes, because of the higher attenuation occurring for rockfall events, which consists in high frequencies whereas for earthquakes it consists in low frequencies. The other parameter, the shape of waveform of signal trace, showed a significant difference between rockfall and earthquake and that could be a complementary feature to discriminate between both. This analysis of artificial rockfall is a first step helpful to understand the seismic characteristics of rockfall, and useful for rockfall seismic events classification in seismic monitoring of slope.