Community characteristics of wild fruit forests along elevation gradients and the relationships between the wild fruit forests and environments in the Keguqin Mountain region of Iii

In this paper,the quantitative relationship between the wild fruit communities and direct environmental factors is discussed on the basis of detailed data on landscape scale habitats obtained through field vegetation investigation.The results from TWINSPAN and DCCA showed that:1) In the distribution sections of the wild fruit forest in the Keguqin Mountain region,the basic patterns characteristic of the different habitats are due to topographic factors,nutrients and moisture conditions;2) The elevation affected the most basic differentiation of plant communities in the study area,indicating that the elevation condition was the most important factor restricting the distribution of the wild fruit communities in the study area;3) The close relationship between the moisture content in the upper soil layer and the elevation reflected the influence of moisture conditions on both wild fruit and herb-layer communities;4) Nutrient differences not only indicated that the habitat conditions were different in themselves but also showed that the present nutrient conditions of the habitats were seriously affected by human activities.In summary,under complicated mountainous topographic conditions,the habitat conditions for the communities differed very significantly,and the combination of elevation,soil moisture content,total nitrogen,slope aspect,and pH value influenced and controlled the formation of community distribution patterns in the study area.     

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Screening and characterization of the alkaline protease isolated from PLI-1, a strain of Brevibacillus sp. collected from Indonesia’s hot springs

A total of 69 strains of thermophilic bacteria were isolated from water, soil and sediment samples from three Indonesia’s hot spring areas (Pantai cermin, Kalianda and Banyu wedang) by using Minimal Synthetic Medium (MSM). The extreme thermophile Brevibacillus sp. PLI-1 was found to produce extracellular thermophilic alkaline protease with optimal activity at 70℃ and pH 8.0-9.0. The molecular weight of the protease was estimated to be around 56 kD by SDS-PAGE. The maximum activity of the protease was 26.54 U mL-1. The protease activity did not decrease after 30 min and still retained more than 70% of relative activity after 60 min at 70℃ and pH 8.0. The ion Mg2+ was found to promote protease activity at both low and high concentrations, whereas Cu2+ and Zn2+ could almost completely inhibit the activity. Divalent cation chelator EDTA inhibited the enzyme activity by 55.06% ± 0.27%, while the inhibition caused by PMSF, Leupeptin, Pepstain A and Benzamidine were 66.78% ± 3.25%, 52.37% ± 0.25%, 62.47% ± 2.96% and 50.99% ± 0.24%, respectively. Based on these observations, the enzyme activity was conspicuously sensitive to the serine and cysteine protease inhibitors. All these results indicated that the protease isolated from the strain PLI-1 was a thermophilic protease and had a high-temperature stability and a pH stability.     

An AFLP Genetic Linkage Map of Pacific Abalone （Haliotis discus hannai）

A genetic linkage map of Pacific abalone （Haliotis discus hannai） was constructed using AFLP markers based on a two-way pseudo-testcross strategy in a full-sib family. With 33 primer combinations, a total of 455 markers （225 from the female parent and 230 from the male parent） segregated in a 1 ： 1 ratio, corresponding to DNA polymorphism： heterozygous in one parent and null in the other. The female framework map consisted of 174 markers distributed in 18 linkage groups, equivalent to the H. discus hannai haploid chromosome number, and spanning a total length of 2031.4 cM, with an average interval of 13.0 cM between adjacent markers. The male framework map consisted of 195 markers mapped on 19 linkage groups, spanning a total length of 2273.4cM, with an average spacing of 12.9cM between adjacent markers. The estimated coverage for the framework linkage maps was 81.2% for the female and 82.1% for the male, on the basis of two estimates of genome length. Fifty-two markers （11.4%） remained unlinked. The level of segregation distortion observed in this cross was 20.4%. These linkage maps will serve as a starting point for linkage studies in the Pacific abalone with potential application for marker-assisted selection in breeding programs.     

EVIDENCE FOR ABRUPT CLIMATIC CHANGES ONNORTHWESTERN MARGIN OF EAST ASIAN MONSOON REGION DURING LAST DEGLACIATION

Based on investigations of the Zhongwei Nanshan aeolian section situated in the southeastern margin of Tengger Dcsert, carbon-14 and TL (thermoluminescence) dating results and paleoclimatic proxies such as magnetic susceptibility and grain size, we inferred that the northwestern margin of East Asian monsoon region experienced abrupt climatic changes during the last deglaciation. Six oscillation events were identified: Oldest Dryas, Belling, Older Dryas, AllerФd, lntra-AllerФd Cold Period (1ACP) and Younger Dryas (YD). The summer monsoon was weaker during Oldest Dryas and Younger Dryas when the winter monsoon was stronger. However, during the B/A (BФlling/AllerФd) period, the summer monsoon strengthened, reflected by magnetic susceptibility, when the winter monsoon also became strong, which is different from the paleoclimatic pattern established in the East Asian monsoon region. Furthermore,the summer monsoon was nearly in phase with the climate changes inferred from the oxygen isotopic records of Greenland ice cores. It could be speculated that the variations of the sea ice cover in the high latitudes of the North Hemisphere affected the high pressure of Asian continent and the changes of the winter monsoon inland. On the other hand,the sea ice cover variations might have indirectly caused the occurrence of ENSO events that has tightly been related to the summer monsoon in northwest margin of East Asian monsoon region.     

Energy flow in Branchiura sowerbyi (Oligochaeta: tubificidae) in a shallow macrophyte-dominated lake, Biandantang Lake

The energy flow ofBranchiura sowerbyi was studied for the first time in China in a shallow macrophytic lake, Biandantang Lake, Hubei Province. The energy flow was calculated from the measurement of flesh production (12.5241kJ/m²a), egestion (517.7302kJ/m²a), metabolism (38.3273 kJ/m²a), and excretion (4.3798kJ/m²a). The net growth efficiency of the species is about 22.7%, which accords well with the generally reported value for oligochaetes. In addition, the relationship between starvation respiration (R, mgO₂/ind·d), wet weight (Ww, mg) and temperature (T, °C) were also measured, with the regression function beingR=0.008Ww^0.736 e^0.050T. Project supported by NSFC (30270278, 3960019), the foundation of the government of Hubei Province (No. 2000J109), and the foundation of Ecological Station, CAS in the Institute of Hydrobiology.     

THERMAL EFFECTS OF BUILDING＇S EXTERNAL SURFACES IN CITY——Characteristics of Heat Flux into and out of External Wall Surfaces

This study examined the thermal effects of building‘s external wall surfaces, using observational data of spatial-temporal distribution of surface temperature, air temperature, and heat flux into and out of external surface. Results indicate that external wall surface temperature and nearby air temperature vary with the change of orientation, height and season. In general, the external wall surface temperature is lower near the ground, and is higher near the roof, than nearby air temperature. But north wall surface temperature is mostly lower than nearby air temperature at the same height; south wall surface temperature during the daytime in December, and west wall surface temperature all day in August, is respectively higher than nearby air temperature. The heat fluxes into and out of external wall surfaces show the differences that exist in the various orientations, heights and seasons. In December,south wall surface at the lower sites emits heat and north wall surface at the higher sites absorbs heat. In April, all external wall surfaces, emit heat near the ground and absorb heat near the roof. In August, west wall surface all day emits heat, and other wall surfaces just show the commensurate behavior with that in April.     

Methane-rich fluid inclusions and their hosting volcanic reservoir rocks of the Songliao Basin, NE China

Methane-rich fluids were recognized to be hosted in the reservoir volcanic rocks as primary inclusions.Samples were collected from core-drillings of volcanic gas reservoirs with reversed δ12C of alkane in the Xujiaweizi depression of the Songliao Basin. The volcanic rocks are rhyolite dominant being enriched in the more incompatible elements like Cs, Rb, Ba, Th, U and Th with relative high LREE, depleted HREE and negative anomalies of Ti and Nb,suggesting a melt involving both in mantle source and crustal assimilation. Primary fluids hosted in the volcanic rocks should have the same provenance with the magma. The authors concluded that the enclosed CH4 in the volcanics are mantle/magma-derived alkane and the reversed δ13C of alkane in the corresponding gas reservoirs is partly resulted from mixture between biogenic and abiogenic gases.     

多级次地下水流系统的最小能耗率原理初探

20世纪60年代初期,Tóth基于定水头上边界条件推导出解析解,得出多级次地下水流系统,是水文地质学里程碑式的突破,成功地解决了一系列理论和实际问题。但Tóth解析解存在的缺陷也长期沿袭：单纯重视数学模拟而忽视物理机制;将地形控制地下水位看成是普适性规律;忽视给定水头上边界数学模拟的失真。这些缺陷,尤其是忽视物理机制探究,不仅妨碍Tóth理论自身发展,而且导致地下水流系统理论尚未被国际水文地质界普遍接受。参照河流动力学中应用的最小能耗率原理,类比提出地下水流最小能耗率的表达式。基于已有的通量上边界地下水流模式数值模拟结果,进一步探究物理机制,归纳得出地下水流系统遵循最小能耗率原理的结论。