Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

建鲤基因组DNA的RAPD分析

用 1 5个随机引物对建鲤基因组DNA进行RAPD分析 ,结果显示 ,建鲤群体内的遗传相似系数为 0 91 81± 0 0 73 8,多态位点比例为 0 41 67,平均杂合度为 0 1 884。表明建鲤种质较纯 ,群体内的遗传变异程度较小。建鲤优良性状产生的分子学基础是等位基因的杂合程度较高     

农村信用社产权制度创新研究

现行农村信用社存在的出资人所有权虚置、收益权削弱、法人财产权丧失等产权制度缺陷 ,严重阻碍着它的进一步发展。形成这些缺陷的主要原因在于农村信用社产权改革的方向不明和对产权范畴的片面理解 ,因此 ,农村信用社产权制度创新应在清晰界定产权的基础上、向股份制、股份合作制、合作制等多种产权形式的方向发展。     

LHRH-A和hCG对南方鲶血清促性腺激素水平的影响

运用放射免疫分析法测定了促黄体释放激素类似物 (LHRH A)和人绒毛膜促性腺激素(hCG)对南方鲶血清促性腺激素 (GTH)水平的影响 ,结果表明 :LHRH A和hCG都能提高南方鲶一龄幼鱼血清GTH水平 ,hCG与LHRH A结合注射后血清GTH水平显著高于单独注射hCG或LHRH A后的血清GTH水平。LHRH A和hCG结合注射可诱导性成熟南方鲶排卵 ,同时排卵过程中伴随着血清GTH水平的急剧上升 ,在排卵后迅速下降 ;注射同样剂量的药物而未排卵的血清GTH水平虽有升高 ,但升高的幅度不及排卵鱼。这种血清GTH水平的急剧增高对诱导南方鲶卵母细胞最后成熟和排卵具有重要作用。     

EFFECTS OF FISH OIL,DHA OIL AND LECITIHIN IN MICROPARTICULATE DIETS ON STRESS TOLERANCE OF LARVAL GILTHEAD SEABREAM（SPARUS AURATA）

The effects of natural fish oil,DHA oil and soybean lecithin in microparticulate diets on stress tolerance of larval gilthead seabream(Sparus aurata)were investigated after 15 days feeding trials.The tolerance of larval gilthead seabream to various stress factors such as exposure to air(lack of dissolved oxygen),changes in water temperature(low)and salinity(high) were determined.This study showed that microparticulate diet with natural fish oil and soybean lecithin was the most effective for in-creasing the tolerance of larval gilthead seabream to various stresses,and that microparticulate diet with natural fish oil and palmitic acid(16:0)was more effective than microparticulate diet with DHA oil and soybean lecithin.     

青藏高原隆升的非线性动态有限元仿真研究

根据青藏高原的地质特征建立分析模型，采用3维动态有限元方法，在计算仿真板块速度场的基础上，计算在青藏高原的隆升过程中该地区地壳岩石的等效应力和位移随时间的变化，计算仿真得到的速度场与1998年GPS观测的速度场吻合良好；与过去一贯的假设相反，计算结果反映出地壳应力场不是静态的，而是此起彼伏，不断变化的，应力值最大且变化最剧烈的地区在克什米尔地区、鄂尔多斯地区和鲜水河－小江断裂带，与地震多发区域吻合。     

RECENT TECTONIC DEFORMATION ANOMALY AND EARTHQUAKES IN GANSU-NINGXIA-QINGHAI AREA

1　IntroductionAccordingtogeologicalstructure ,theGansu Ningxia Qinghaiareabelongstothenortheastmar ginofQinghai Tibetblock .Thisareahasbeenpaidmuchattentionby geo specialistsinChinaandabroadbecauseofitssignificanttectonicmovement,itsintensiveseismicity ,anditsimportanceinearth quakehazardmitigation .IntheDevelopmentPro gramonNationalKeyBasicResearchesundertheProject“MechanismandPredictionofContinentalStrongEarthquakes”,themechanismsofcontinen talstrongearthquakesarestudied ,usinghypo…     

SPATIAL—TEMPORAL VARIATION OF HEAVY METAL ELEMENTS CONTENT IN COVERING SPIL OF RECLAMATION AREA IN FUSHUN COAL MINE

Grid method is employed for sampling covering soil at the test field,which is reclamation area filled by coal mining wastes for cropland in the Fushun coal mine,Liaoning Province,the Northeast China.The soil samples are taken at different locations,including three kinds of covering soil,three different depths of soil layers and four different covering ages of covering soil.The spatial-temporal variation of heavy metal element content in reclamation soil is stud-ied.The results indicate that the content of heavy metal elements is decreasing year after year,the determinant reason why the content of heavy metal elements at 60cm depth layer is higher than that at 30cm depth layer and surface is fertiliz-er and manure application;the metal elements mainly come from external environment;there is no metal pollution coming from mother material (coal mining wastes)in plough layer of covering soil.     

Small hypervelocity particles captured in aerogel collectors: Location,extraction, handling and storage

Abstract— It has now been about a decade since the first demonstrations that hypervelocity particles could be captured, partially intact, in aerogel collectors. But the initial promise of a bonanza of partially‐intact extraterrestrial particles, collected in space, has yet to materialize. One of the difficulties that investigators have encountered is that the location, extraction, handling and analysis of very small (10 μm and less) grains, which constitute the vast majority of the captured particles, is challenging and burdensome. Furthermore, current extraction techniques tend to be destructive over large areas of the collectors. Here we describe our efforts to alleviate some of these difficulties. We have learned how to rapidly and efficiently locate captured particles in aerogel collectors, using an automated microscopic scanning system originally developed for experimental nuclear astrophysics. We have learned how to precisely excavate small access tunnels and trenches using an automated micromanipulator and glass microneedles as tools. These excavations are only destructive to the collector in a very small area—this feature may be particularly important for excavations in the precious Stardust collectors. Using actuatable silicon microtweezers, we have learned how to extract and store “naked” particles—essentially free of aerogel—as small as 3 μm in size. We have also developed a technique for extracting particles, along with their terminal tracks, still embedded in small cubical aerogel blocks. We have developed a novel method for storing very small particles in etched nuclear tracks. We have applied these techniques to the extraction and storage of grains captured in aerogel collectors (Particle Impact Experiment, Orbital Debris Collector Experiment, Comet‐99) in low Earth orbit.     

对青藏东北缘现今块体划分、运动及变形的初步研究

利用2维非连续变形分析方法（DDA），以位移代替围压作为边界约束力，研究青藏东北缘现今块体划分及其运动变形。根据该地区地质构造及地震活动，以GPS点测量位移作为模拟结果约束点，得出了较合理的块体划分模型和随时间演化的主应变分布图，并把应变高值区与近几年来发生的5级以上地震作对比，得出了研究区内地震危险性可能较大的区域。另外，对模拟的甘青块体与阿拉善块体的边缘带断裂左旋运动做了大概计算。