条斑紫菜高纯度总DNA及其质粒状DNA的提取

提取条斑紫菜高纯度总DNA及其质粒状DNA的新方法。先用海螺酶处理紫菜叶状体制备细胞,然后用SDS-蛋白酶K裂解细胞提取总DNA,再用玻璃粉浆(glassmilk)对其纯化,经纯化后的总DNA能被EcoRI,Dral与HaeⅢ等限制酶完全酶切,并在酶切图谱上形成明显的DNA带型。当用异硫氰酸胍一十二烷基肌氨酸钠裂解紫菜细胞时,在总DNA提取物中直接发现有一条质粒状DNA带(2.3Kb),即建立了一种极简便的质粒状DNA提取方法。     

Geo-tectonic conditions of the formation of Proterozoic large and superlarge ore deposits along northwestern margin of North China Plate

Four large and superlarge copper-multimetal ore deposits hosted in the Mid-Proterozoic Zhartaishan Group along northwestern margin of North China Plate are stratabound hydrothermal sedimentary deposits, and these deposits formed in mid-late stage of the Mid-Proterozoic Langshan-Zhartaishan rifting system. Four necessary conditions are proposed for the formation of these large and superlarge ore deposits: (i) favorable geological background and tectonic environment; (ii) abundant sources of metallogenic material; (iii) favorable sedimentary lithofacies and paleogeographic conditions; (iv) sudden change events during metallogenic processes.     

Simultaneous simulation of the three components of seismic accelerograms at locations around seismological stations

Journal of Seismology - This paper proposes a new numerical procedure, based on an evolutionary optimization algorithm, for the simultaneous generation of the three components of the seismic ground...     

山西沁水盆地中-南部煤储层渗透率物理模拟与数值模拟

通过对山西沁水盆地中南部上主煤层宏观裂隙观测,力学参数测量和应力渗透率实验,分别建立了裂隙面密度、裂隙产状、裂隙宽度与煤储层渗透率之间的预测数学模型;利用FLAC—3D软件,模拟了该区上主煤层内现代地应力状态,结合煤层气试井渗透率资料,构建了应力与渗透率之间关系预测的数学模型,并对该区上主煤层渗透率进行了全面预测。通过吸附膨胀实验,揭示了各煤类煤基质的收缩特征,构建了有效应力、煤基质收缩与渗透率之间的耦合数学模型,并对煤层气开发过程中渗透率动态变化进行了数值模拟。     

锯缘青蟹纳精囊精子顶体酶活力研究

顶体酶是精子顶体部特有的胰蛋白酶,其在顶体反应、精子和透明带的结合及穿透中起重要作用.本文采用测定精子顶体氨基化酶活力方法来测定锯缘青蟹（Scylla serrata）精子的顶体酶活力.据测定,青蟹纳精囊精子项体酶活力分别为3.566 μIU/10^6和4.248 μIU/10^6.青蟹精子顶体酶活力和用同样方法测定的其它物种（如猪、人、马）相比较低.这反应了不同物种之间的精子顶体酶活力存在着较显著的差异,也反应青蟹纳精囊中精子处于一种抑制状态.这种精子活力抑制方式可能有利于精子在纳精囊中的长期存活.本实验结果也说明,临床应用的顶体酶测定方法也可以用于蟹类精液质量的评价.     

“进源找油”：论四川盆地非常规陆相大型页岩油气田

源岩油气规模快速发展,是中国能源结构转型的重要战略选项;陆相页岩油和气是中国源岩油气最具潜力的组成类型,是中国陆上未来"进源找油"最重要的突破对象。本文首次研判提出四川盆地侏罗系油和气发展,应尽快由常规构造-裂缝石油、致密灰岩与砂岩油向孔隙型页岩油气转变,重点阐述了大安寨段页岩油气4项新进展:①评价侏罗系4套富有机质页岩总生油量245×10~8t,石油总资源量172×10~8t,还有相当规模的天然气资源,既富油又富气,其中67%的油气资源集中在大安寨段;②大安寨段二亚段是侏罗系页岩油和气的"甜点段",黑色页岩厚度一般20～80m,TOC平均值大于2%,Ⅱ_1～Ⅱ_2型干酪根,R_o主体1.1%～1.4%,处于轻质油—凝析油气窗口,页岩储层孔隙度4%～6%,页理裂缝发育,脆性矿物含量一般在50%以上,压力系数1.2～1.8,滞留烃量多为100～200mg/g·TOC,油质较轻,地层埋深较浅,具备与北美页岩油气媲美的地质工程条件;③优选出页岩油气"甜点区"评价的3项核心参数(R_o、富有机质页岩厚度、埋藏深度),评价出大安寨段二亚段页岩油"甜点区"主要分布在盆地中北部地区,页岩厚度20～50m,面积约2×10~4km~2;④提出了加快页岩油和气以水平井体积压裂为核心的风险勘探和开发试验、加强全层系资源评价、加速准备先导试验技术方案等建议,力争推动侏罗系孔隙型页岩油和气突破发现。四川盆地侏罗系陆相页岩油和气新认识,可能为真正开启侏罗系源岩油气"进源找油"、规模开发新征程提供重要的理论依据。     

论东秦岭前寒武纪的构造演化特征

本文研究的范围主要涉及东秦岭的北部地区——北秦岭(即旧称“秦岭地轴”)。作者着重讨论了秦岭造山带的杂岩(秦岭杂岩、宽坪杂岩和陶湾杂岩)和太华杂岩的岩石组合、原岩建造、岩石化学、地球化学技其地质构造特征。提出秦岭杂岩是在中元古代时期因华南洋壳海域的岩石圈断块沿华北断块南缘向北俯冲引起断块区基底开裂,向南漂离而形成的块体。宽坪杂岩与陶湾杂岩是在秦岭杂岩块体向南漂离过程中形成的以洋壳为底的边缘海盆地的产物。经晋宁运动,边缘海闭合,形成早期的北秦岭造山带。     

Effects of biogenic amines on the testicular development in mud crabs Scylla serrata

1 IntroductionBiogenicamine, oneofim portantbioactivesub-stancesin crustaceans,participatesin extensivephysi-ologicalactivities, suchastegum entarycolorchange,m olting,m usclecontraction,glucidem etabolism,etc.(Fingerman etal.,1994).Furthermore,ithasbeen …     

Structures of ovary and ovarian follicle in flathead lobster, Thenus orientalis (Lund, 1793) (Crustacea: Decapoda: Scyllarida)

ImeIOWThe nathead lobster, Thenus orientalis, is widely distributed in the trOPical and subtIDPical indO - West Pacific region. T. orientalis is one of the most eCOnondcally significantspecies among scyllarids althoUgh its catch is nowhere large. In addition to its eCOnondc value,T. Orientalis is also of great scientific value because it represents a fdrily wfor reproductivebiology is ~ly studied. It is important to clarify the structures of ovary and Ovarian follicle inT. Orientalis.…