平端深海偏顶蛤(Gigantidas platifrons)实验室蓄养过程鳃部含菌上皮细胞超微结构动态变化研究

平端深海偏顶蛤(Gigantidasplatifrons)是南海台西南冷泉区的典型优势物种,鳃丝上皮细胞内共生大量甲烷氧化菌,通过甲烷有氧氧化合成有机物为共生体系提供物质能量,是平端深海偏顶蛤赖以生存的重要能量来源器官,溶酶体在共生体系的营养互作和稳态维持中可能发挥重要作用,本研究使用电镜技术观测了常压培养过程中(0d,30d,90d)平端深海偏顶蛤共生体系中共生菌和溶酶体的动态变化,通过鳃上皮含菌细胞超显微结构的变化研究常压培养对深海共生体系的影响,并探讨溶酶体在宿主—共生菌营养传递和共生菌群稳态维持中的关键作用。研究发现,在原位状态样品中(0d)共生菌和溶酶体呈极化分布,细胞结构完整清晰;蓄养30d后,含菌细胞出现明显破碎,共生菌数量大幅降低,溶酶体数量、范围和消化程度大幅增加,对细胞顶端的甲烷氧化菌进行分解;而90d后,共生菌在溶酶体的作用下消失殆尽,鳃部有明显细胞脱落后留下的坑洞,细胞呈现空泡状,无法明确区分各种细胞组分。上述结果展示了长期常压蓄养过程中鳃上皮含菌细胞中溶酶体与甲烷氧化菌的动态变化,推测当共生菌丢失后溶酶体也同步降低活跃度,平端深海偏顶蛤在共生互作中通过溶酶体主导消化和调控共生菌。     

獐子岛养殖区夏季底层水温快速变化的机理分析

Rapid changes in the near-bottom water temperature are important environmental factors that can significantly affect the growth and development of species in the bottom culture. The object of this research is to investigate the mechanism causing these rapid changes within a bottom culture area near the Zhangzi Island. The hydrographic transects observations in the North Yellow Sea(NYS) suggest that our mooring station is very close to the tidal mixing front. The horizontal advection of the tidal front has induced the observed tidal change of bottom temperature at the mooring station. Analysis of the mooring near-bottom temperature and current measurements show that the angle between the tidal current horizontal advection and the swing of the tidal front is crucial in determining the variation trend of temperature. When the angle equals 90°, the horizontal tidal current advects along the isotherms so the temperature remains the same. When the angle is between 0° and 90°, the seawater moves from deep water to the warmer coastal zone and the temperature decreases. In contrast, the horizontal tidal advection moves the coastal warm water to the mooring station and the water temperature increases when the angle is between 90° and 180°. The amplitude of the temperature change is proportional to the magnitude of the horizontal temperature gradient and the tidal excursion in the direction of the temperature gradient. This study may facilitate the choice of culture area in order to have a good aquaculture production.     

Feeding intensity and molecular prey identification of the mantis shrimp,Oratosquilla oratoria (De Haan, 1844), in the Tianjin coastal zone of Bohai Bay

Bohai Bay, a representative semi-enclosed bay of China, has been severely impacted in recent decades by rapid human development. Although the mantis shrimp Oratosquilla oratoria plays an important role in the Bohai Bay ecosystem and fishery, its feeding ecology and the impact of habitat changes on its feeding habits are poorly known. In this study, we sought to identify its feeding strategy through the separation of stomach contents, and to describe its trophic ecology during maturation in the Tianjin coastal zone of Bohai Bay by the DNA barcoding method using a fragment (about 690 bp) of the mitochondrial cytochrome oxidase I (CO I) gene. A total of 594 specimens were collected from March to July 2018, and 347 (58.42%) stomachs were found to have food remains. More than half of the O. oratoria individuals had poor feeding activity, and the degree of feeding of females was higher than that of males, but there was no significant difference in the visual fullness index and the fullness weight index (FWI) between sexes for each month. In addition, the feeding activity of O. oratoria was consistent across the months, suggesting in artificial breeding programs it is not worried too much about the accessional diet supplied to the female bloodstocks except maintaining their normal food. A total of 167 retrieved prey items yielded 187 readable sequences; 86.63% of these sequences could be assigned to the species level. Consequently, 25 different prey taxa covering all readable sequences were identified, showing O. oratoria is a generalist predator. In terms of percent composition by frequency of occurrences (%N), crustaceans and fishes accounted for the same and highest percentage (35.83%) in the mantis shrimp diet, followed by mollusks (24.59%) and the remaining marine organisms (3.73%) including Polychaeta (2.67%), Sagittoidea (0.53%), and Chromadorea (0.53%). The result shows the benthic fish Oxudercidae (family) appears to be an especially important food supply for O. oratoria during maturation. The degree of cannibalism decreased with an increase in diet species and the frequencies of cannibalism in this study was noticeably higher than that in previous studies, suggesting that cannibalism may be a significant feeding strategy in the mantis shrimp O. oratoria in the Tianjin coastal zone of Bohai Bay, and because of the frequently cannibalistic behavior, the high stocking density of broodstocks should be avoided.     

长江口咸潮入侵变化特征及成因分析

本文基于海洋站潮位观测数据、海平面变化影响调查信息以及长江口水文站径流量数据等,重点分析了2009?2018年长江口咸潮入侵的变化特征及其影响因素,分析结果表明:(1)长江口咸潮入侵季节变化特征明显。咸潮一般从每年的9?10月开始入侵,翌年4?5月结束。3月咸潮入侵次数最多,达12次。2009?2018年,长江口咸潮入侵次数和咸潮持续时间均呈下降趋势,2009年长江口咸潮入侵次数最多,达13次,时间均发生在10月至翌年的4月;咸潮持续时间年际变化较大,2011年咸潮入侵持续时间最长,累计为55 d。2015?2018年,咸潮入侵次数和入侵持续时间均明显减少,2018年没有监测到咸潮入侵过程。(2) 1?4月,长江口处于季节性低海平面期,且同期径流量少,但是受东亚季风影响,持续的增水过程使得增减水?径流量综合影响指数明显偏高,其中1月、2月、3月的影响指数分别为1.5、1.9和1.6,该时段长江口的咸潮入侵过程主要受增减水的影响。5?7月,长江口径流量明显增加,海平面?径流量综合影响指数均小于0,径流的作用强于海水上溯。8月,长江口径流量开始下降,虽然季节海平面较高,但是长江口呈现明显的减水过程,海平面?径流量和增减水?径流量的综合影响指数分别为0.1和?1.6,基本不会发生咸潮入侵。9月,长江口处于季节高海平面期,并且以增水为主,海平面?径流量和增减水?径流量的综合影响指数较大,分别为1.2和1.0,易发生咸潮入侵。10月、11月长江口海平面?径流量的综合影响指数分别为1.5和0.8,径流影响弱于海水上溯,易发生咸潮入侵。(3) 2009?2018年发生的48次咸潮入侵过程有2/3恰逢天文大潮。在某些年份长江口沿海基础海平面偏高,若持续增水恰逢天文大潮,则加剧咸潮入侵的影响程度。     

Influence Analysis and Vibration Restraint Solutions Research on the Underwater Acoustic Monitoring System

China Ocean Engineering - In order to reduce the hydrodynamic and structural influences on the detection accuracy especially in the very-low-frequency range, some vibration restraint methods are...     

基于四叉树的多波束水深数据快速检索方法

针对水下地形测量仿真器中的多波束测深结果仿真环节中,对海量已知水深点云数据进行处理时存在的检索效率低、内存占用大的问题,设计了一种基于四叉树的数据处理方案。以四叉树数据结构并采用序列化方式存储索引文件,提高点云数据的检索效率;通过内存映射的方法读取海量点云数据,减少内存占用。数据实验表明:相对于常见的遍历检索,在检索点云数不超过总点云数的约四分之三时,四叉树水深点云检索效率提高了1倍以上;在检索点云数越少,总点数云越多时,四叉树水深点云检索的效率最多可提升30倍以上。基于四叉树的数据处理算法可有效地提高点云数据的检索效率,适合于多波束测深结果仿真过程中的海量点云数据的处理。     

中国东部海域冬季风场与海浪场的关联特征分析

利用WAVEWATCHⅢ海浪模式模拟的1993-2011年中国东部海域19 a冬季逐日海浪场资料以及同期CCMP逐日风场资料,采用奇异值分解(SVD)的方法分析了冬季中国东部海域海浪场与提前0~5 d的东亚大陆地面风场的关联特征。结果发现:海浪场与提前1 d的地面风场的关联更有意义;SVD第一模态和第二模态分别反映了贝加尔湖以东南下的反气旋式偏北扰动大风(或气旋式偏南扰动大风)和中国东部平原入海的气旋式扰动风场(或反气旋式扰动风场)对中国东部海域海浪的扰动影响。此外,SVD分析还揭示了冬季影响中国东部海域海浪的大风关键区和移动路径;随着时间的推移,大风关键区从贝加尔湖以东逐步由蒙古南下影响中国东北和华北地区,最后到达中国东部海域。     

雅浦海沟海参(Synallactidae)肠道微生物多样性及系统进化分析

为研究超深渊海参肠道微生物多样性,了解其所在沉积环境的物质循环特征,利用MiSeq高通量测序技术,对雅浦海沟海参肠道微生物16S rDNA的V3-V4和V4-V5可变区基因序列进行了扩增,并与近岸养殖刺参(Apostichopus japonicus)肠道微生物的多样性和组成进行比较。实验结果显示,与养殖刺参比雅浦海沟海参肠道细菌的多样性(辛普森指数和Chao1指数)和丰富度(ACE指数和香农指数)均较低;雅浦海沟海参肠道细菌主要包括γ-变形菌(Gammaproteobacteria,34.1%)、α-变形菌(Alphaproteobacteria,16.0%)和拟杆菌(Bacteroidetes,24.0%),古菌主要由奇古菌门(Thaumarchaeota,98.8%)组成;雅浦海沟海参肠道中,化能自养的氨氧化古菌亚硝化侏儒菌属(Nitrosopumilus)相对丰度达95.6%,甲基营养的硝酸盐还原菌(Methylotenera)相对丰度为10.6%;而养殖海参肠道中硫酸盐还原菌(28.1%)和硫氧化菌硫卵形菌属(Sulfurovum,13.7%)的相对丰度较高。16S rDNA基因序列的系统进化分析结果表明,亚硝化侏儒菌与超深渊沉积环境中奇古菌门亲缘关系最近,且遵循氨氧化古菌从陆地到深海进化的理论;雅浦海沟海参和养殖海参的肠道微生物组成差异可能反映了超深渊和近海沉积环境中氮循环、硫循环特征。本研究可以为揭示超深渊物质循环和能量流动及生命演化提供参考。     

安徽巢湖凤凰山晚古生代大冰期沉积特征与碳同位素变化

地史中海相碳酸盐岩记录的无机碳同位素的波动经常与全球气候事件相互关联,但碳酸盐岩的碳同位素不仅与全球碳循环相关,也受控于区域水循环和成岩作用.晚古生代大冰期在石炭纪末—早二叠世达到最高峰,南方冈瓦纳大陆高纬度地区冰川的进退引起低纬度地区旋回性的海平面变化,并发育显著的沉积间断,在华南表现为喀斯特岩溶角砾岩等.本文选取安...     

LANDFORM MORPHOLOGIC RELATIONS BETWEEN INTER-GULLIED AND GULLIED LAND AREAS--A CASE STUDY OF THE WANGJIAGOU WATERSHED IN THE WEST OF SHANXI PROVINCE

Previous studies on erosional relations between inter-gullied and gullied lands have mainly concentrated on exploration of water and sediment relations, with few quantitative studies of evolution relations between inter-gullied land and gullied landform erosion in small catchments. This paper, using information from a 1:5000 digital orthophotomap (DOM), digital elevation model(DEM), and topographic map of the same period and of the same scale, quantitatively analyzes the impact and interactions of inter-gullied and gullied land geomorphologic characteristics on the dissected extent of a catchment using orthogonal polynomial regression analysis. Results indicate that gullies play a leading role in the catchment morphologic evolution and extent of cracked ground surface. When inter-gullied land areas are the same, a 0. 1 km2 gullied land area has the maximum effect on the erosive evolution intensity of the catchment cracked degree. The smaller the catchment area is, the greater the extent of cracked ground surface and gully intensity would be. The geomorphologic evolution mechanism of gullied catchments can be explained as a function of geomorphologic indicators of inter-gullied and gullied land characteristics.