Establishment and characterization of a testicular Sertoli cell line from olive flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>

The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.     

Impact factors identification of spatial heterogeneity of herbaceous plant diversity on five southern islands of Miaodao Archipelago in North China

Herbaceous plants are widely distributed on islands and where they exhibit spatial heterogeneity. Accurately identifying the impact factors that drive spatial heterogeneity can reveal typical island biodiversity patterns. Five southern islands in the Miaodao Archipelago, North China were studied herein. The spatial distribution of herbaceous plant diversity on these islands was analyzed, and the impact factors and their degree of impact on spatial heterogeneity were identified using CCA ordination and ANOVA. The results reveal 114 herbaceous plant species, belonging to 94 genera from 34 families in the 50 plots sampled. The total species numbers on different islands were significantly positively correlated with island area, and the average α diversity was correlated with human activities, while the β diversity among islands was more affected by island area than mutual distances. Spatial heterogeneity within islands indicated that the diversities were generally high in areas with higher altitude, slope, total nitrogen, total carbon, and canopy density, and lower moisture content, pH, total phosphorus, total potassium, and aspect. Among the environmental factors, pH, canopy density, total K, total P, moisture content, altitude, and slope had significant gross effects, but only canopy density exhibited a significant net effect. Terrain affected diversity by restricting plantation, plantation in turn influenced soil properties and the two together affected diversity. Therefore, plantation was ultimately the fundamental driving factor for spatial heterogeneity in herbaceous plant diversity on the five islands.     

华南埃迪卡拉(震旦)系顶部地层划分及与寒武系界线FAD分子的选择

"雪球事件"以来,早期生命呈现多层次、大规模的加速演化,到寒武纪前夕,生命大爆发达到高潮;在生命剧烈演替的同时,地球环境标志也发生着响应,碳同位素剧烈漂移,并且与各个生命演化节点相对应。但是在划分最重要的地质界线,埃迪卡拉(震旦)系——寒武系界线及界线层型和点位("金钉子",GSSP)确立的问题上迄今仍存在着较大的分歧,以遗迹化石Treptichnus pedum作为该GSSP的标志化石,因其跨系、跨阶产出,生物延限长,因而在全球的生物地层划分对比中(特别在西伯利亚和中国华南地区)一直质疑声不断,而且以遗迹化石作为标志化石本身也具有一定的缺陷。中国华南地区两类古老的宏体化石——矿化管状的Cloudina和条带状的Shaanxilithes在全球范围内可以很好的对比,并且这两种实体化石的出现都对应着碳同位素的明显正漂移。本文通过研究华南震旦系顶部化石带的地层分布和地理分布,综合分析了其时空分布特点和系统演化意义,深入探讨了Cloudina和Shaanxilithes作为寒武系底界标志化石所具备的潜力,对埃迪卡拉(震旦)纪—寒武纪地层界线划分和层型选择提出了更为理想的标准。     

新疆乌鲁木齐东部早二叠世枕状玄武岩火山岩系的发现及大地构造意义

新疆乌鲁木齐东部野生动物园附近的下二叠统下部发育了一套以枕状玄武岩为代表的火山岩系。该玄武岩在公路上发育有两层,上部一层较厚,可达7～8m,下部一层2～3m。岩枕近圆形、肾状、枕状、条带状或蠕虫状等,多呈顶突底凹。岩枕长轴平行排列,长轴基本顺层面分布;有的岩枕中含大量海百合茎、珊瑚及腕足等生物化石。在TAS图中大部分样品位于玄武粗安岩,而在K_2O-SiO_2图上主要落在低K拉斑玄武岩区间。MnOTiO_2-P_2O_5图解显示以岛弧为主,常量元素的特征总体上更接近岛弧玄武岩。在Hf/3-Th-Ta图解上也以岛弧为主;在Zr/4-2Nb-Y图解显示以火山弧为主,常量元素的特点总体上更接近岛弧玄武岩。微量元素原始地幔标准化蛛网图表明为同源岩浆产物,具高度相似的演化过程,多种微量元素判别图揭示岛弧-弧后盆地环境;稀土总量明显较低,在稀土元素球粒陨石标准化图解上,其稀土分布曲线一致性较好,呈轻稀土富集右倾型,Eu为负异常,揭示了乌东玄武岩岩浆有一定分异。稀土元素配分曲线与弧后盆地玄武岩(BABB)具有很好的一致性。U-Pb和谐年龄为283±8Ma,结合地层及古生物资料推测乌东玄武岩喷发的时间为早二叠世早期。乌东枕状玄武岩-灰岩之下有一套(磨拉石)底砾岩,初定为石炭-二叠系的界限。底砾岩之下为下石炭统的中厚层灰岩,具有明显的喀斯特化,为不整合接触,揭示在两者之间发生了造山作用。通过对新疆乌东早二叠世早期的枕状玄武岩的地质特征、地球化学特征、形成环境和时代的研究,表明乌东一带早二叠世主要为一个岛弧和弧后盆地环境,进一步揭示了北天山北缘石炭-二叠世碰撞闭合造山之后又发生了松弛扩张形成了弧后盆地,海水再度大规模入侵。最终的闭合碰撞造山的时间最早可能在晚二叠世。由于乌东枕状玄武岩在喷出地表后受到了生物化石和陆源碎屑的污染,再加上侵入过程中地壳的污染,使其具有非常复杂的地球化学特点和多解性。乌东玄武岩的研究对于了解博格达山、甚至天山中段在晚古生代的构造沉积演化及造山作用具有重要意义,同时对准噶尔盆地、吐哈盆地及三塘湖盆地油气资源的形成与分布具有重要意义。     

Tectono–Thermal Evolution, Hydrocarbon Filling and Accumulation Phases of the Hari Sag, in the Yingen–Ejinaqi Basin, Inner Mongolia, Northern China

This work restored the erosion thickness of the top surface of each Cretaceous formations penetrated by the typical well in the Hari sag, and simulated the subsidence burial history of this well with software BasinMod. It is firstly pointed out that the tectonic subsidence evolution of the Hari sag since the Cretaceous can be divided into four phases: initial subsidence phase, rapid subsidence phase,uplift and erosion phase, and stable slow subsidence phase. A detailed reconstruction of the tectonothermal evolution and hydrocarbon generation histories of typical well was undertaken using the EASY R_0% model, which is constrained by vitrinite reflectance(R_0) and homogenization temperatures of fluid inclusions. In the rapid subsidence phase, the peak period of hydrocarbon generation was reached at c.a.105.59 Ma with the increasing thermal evolution degree. A concomitant rapid increase in paleotemperatures occurred and reached a maximum geothermal gradient of about 43-45℃/km. The main hydrocarbon generation period ensued around 105.59-80.00 Ma and the greatest buried depth of the Hari sag was reached at c.a. 80.00 Ma, when the maximum paleo-temperature was over 180℃.Subsequently, the sag entered an uplift and erosion phase followed by a stable slow subsidence phase during which the temperature gradient, thermal evolution, and hydrocarbon generation decreased gradually. The hydrocarbon accumulation period was discussed based on homogenization temperatures of inclusions and it is believed that two periods of rapid hydrocarbon accumulation events occurred during the Cretaceous rapid subsidence phase. The first accumulation period observed in the Bayingebi Formation(K_1 b) occurred primarily around 105.59-103.50 Ma with temperatures of 125-150℃. The second accumulation period observed in the Suhongtu Formation(K_1 s) occurred primarily around84.00-80.00 Ma with temperatures of 120-130℃. The second is the major accumulation period, and the accumulation mainly occurred in the Late Cretaceous. The hydrocarbon accumulation process was comprehensively controlled by tectono-thermal evolution and hydrocarbon generation history. During the rapid subsidence phase, the paleo temperature and geothermal gradient increased rapidly and resulted in increasing thermal evolution extending into the peak period of hydrocarbon generation,which is the key reason for hydrocarbon filling and accumulation.     

内蒙古查干凹陷毛11井发现中元古代晚期变质岩:锆石U-Pb定年证据

正1研究目的(Objective)内蒙古查干凹陷位于狼山以西、恩格尔乌苏断裂带以北,从构造单元上属于阿拉善地块。阿拉善地块元古宙归属问题还存在争论,有的观点认为属于华北克拉通西北缘的一部分;另一种观点认为属塔里木或者扬子克拉通。本文通过对查干凹陷毛11钻井下变质岩锆石年代学研究,为阿拉善地块基底归属提供新的年代学证据,同时为阿拉善地区提供一个新的基础资料。     

川南地区海相页岩气中发现微量硫化氢

正1研究目的(Objective)自2011年在四川南部地区下古生界海相地层中获得页岩气工业性突破以来,在四川盆地进行的海相页岩气开发得到多方的重视和迅速发展,随之而来的是人们对页岩气勘探开发过程中对环境影响的关注。页岩气,它以吸附或游离状态为主要方式赋存于富有机质泥页岩及其夹层中,是一种非常规天然气。与常规天然气相比,页岩气气质优良,甲烷含量更高。页岩气化学成分主要为甲烷(CH4),一般含量在85%以上,最高达到99.8%,另外还含有少量的乙烷(C2H6)、丙烷(C3H8)和丁烷(C4H10)。一般认为我国页岩气中可以存在少量氮     

广西平果县发现大面积富硒土壤与富硒火龙果

正1研究目的(Objective)元素硒(Se)是世界卫生组织(WHO)确定的人体必需微量元素。研究表明硒具有抗氧化、抗衰老、抗辐射、抗病毒、保护视力以及提高人体免疫力功能,还能预防和抑制镉、砷、汞等有毒重金属元素对机体的伤害。硒属于分散元素,其在大陆地壳中含量很低,且分布极不均匀。中国处于地球低硒带,全国72%国土面积存在不同程度的缺硒现象。     

“老脚印”对自升式钻井平台插桩滑移的影响研究

渤海海域自升式钻井平台作业频率高,在同一区块多次插桩作业情况多,为自升式平台插桩作业带来严重的滑移风险。通过建立常用的两种类型桩靴的有限元分析模型,采用模拟计算和对比分析的研究方法,对在“老脚印”不同距离位置插桩时,桩基土体的塑性破坏特性、运移规律及桩靴所受侧向滑移力进行了模拟研究,得出了在不同土质条件下,桩基土和桩靴的受力特性及规律,分析了不同位置的“老脚印“对自升式平台插桩滑移风险的影响。